Bioresources and Bioprocessing
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2024
·Vol. 11
·期 1
·1-10
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IF 4.3
Wang Cong,Chang Dongdong,Zhang Qi,Yu Zhisheng
Lignocellulose pretreated using pyrolysis can yield clean energy (such as bioethanol) via microbial fermentation, which can significantly contribute to waste recycling, environmental protection, and energy security. However, the acids, aldehydes, and phenols present in bio-oil with inhibitory effects on microorganisms compromise the downstream utilization and conversion of lignocellulosic pyrolysates. In this study, we constructed a microbial electrolysis cell system for bio-oil detoxification and efficient ethanol production using evolved Escherichia coli to overcome the bioethanol production and utilization challenges highlighted in previous studies. In electrically treated bio-oil media, the E. coli-H strain exhibited significantly higher levoglucosan consumption and ethanol production capacities compared with the control. In undetoxified bio-oil media containing 1.0% (w/v) levoglucosan, E. coli-H produced 0.54 g ethanol/g levoglucosan, reaching 94% of the theoretical yield. Our findings will contribute to developing a practical method for bioethanol production from lignocellulosic substrates, and provide a scientific basis and technical demonstration for its industrialized application.
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Food Chemistry-X
·
2024
·
IF 6.5
Xiaodong Zhai,Yuhong Xue,Wenjun Song,Yue Sun,Tingting Shen,Xinai Zhang,Yanxiao Li,Fuyuan Ding,Di Zhang,Chenguang Zhou,Muhammad Arslan,Haroon E. Tahir,Zhihua Li,Jiyong Shi,Xiaowei Huang,Xiaobo Zou
Ratiometric fluorescent films with high amine sensitivity and stability were developed to monitor the freshness of beef and pork. Fluorescein isothiocyanate (FITC) and red carbon quantum dots (R-CQD) were used as the amine-responsive indicator and internal reference, respectively. The electrospun films prepared by immobilizing FITC and R-CQD complex (F-R) into polyvinylidene fluoride (PVDF) under 35 %, 55 % and 75 % of relative humidity (RH) were named F-R@PVDF-1, F-R@PVDF-2 and F-R@PVDF-3, respectively. In comparison, the F-R@PVDF-2 film exhibited the highest sensitivity to trimethylamine (TMA), demonstrating a limit of detection ( LOD ) value of 1.59 μM, and meanwhile high stability during storage with Δ E value of 1.99 after 14 days of storage at 4 °C. The F-R@PVDF-2 film also showed a significant fluorescent red-to-brown color change during meat freshness monitoring at 4 °C. Conclusively, this study reported a new ratiometric fluorescent film that can be used to track the freshness of meats in food packaging.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Tingting Wang,Fanghui Ma,Feng Huang,Aomei Zhang,Minghui Yang,Xiaoqing Li,Xiang Chen
Ratiometric sensors with background correction are expected to get around the interference from complicated systems. However, there is currently a scarcity of viable carriers for quickly and easily assembling fluorophores with varying fluorescence emission . In this work, we developed a novel aptamer-based ratiometric fluorescence sensor for analysis of adenosine triphosphate (ATP) based on the properties of aptamer that enable conformational transformations upon binding to the target. The carbon dots (CDs) and thioflavin T (ThT) are employed as the reference and detection signal for the ratio-dependent sensor, which are both integrated with the aptamer. The green fluorescence of ThT was initially turn-on after binding with aptamer and then quenched in the presence of ATP, whereas the red fluorescence of CDs remained practically steady. This approach exhibits remarkable selectivity and can detect ATP in the range of 0.5–60 μM with a detection limit of 0.167 μM. Moreover, the method was successfully applied to the determination of ATP in human serum as well as in A549 cells, demonstrating the potential for medical diagnostic applications.
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CHEMOSPHERE
·
2024
·
IF 8.1
Qiuhui Qian,Qian Pu,Xin Li,XingCheng Liu,Anyu Ni,Xiaowen Han,Zejun Wang,Xuedong Wang,Jin Yan,Huili Wang
Triclosan (TCS), a prevalent contaminant in aquatic ecosystems, has been identified as a potential threat to both aquatic biota and human health. Despite its widespread presence, research into the immunotoxic effects of TCS on aquatic organisms is limited, and the underlying mechanisms driving these effects remain largely unexplored. Herein, we investigated the developmental and immune toxicities of environmentally relevant concentrations of TCS in zebrafish, characterized by morphological anomalies, histopathological impairments, and fluctuations in cytological differentiation and biomarkers following both acute (from 6 to 72/120 hpf) and chronic exposure periods (from 30 to 100 dpf). Specifically, acute exposure to TCS resulted in a significant increase in innate immune cells, contrasted by a marked decrease in T cells. Furthermore, we observed that TCS exposure elicited oxidative stress and a reduction in global m 6 A levels, alongside abnormal expressions within the m 6 A modification enzyme system in zebrafish larvae. Molecular docking studies suggested that mettl3 might be a target molecule for TCS interaction. Intriguingly, the knock-down of mettl3 mirrored the effects of TCS exposure, adversely impacting the growth and development of zebrafish, as well as the differentiation of innate immune cells. These results provide insights into the molecular basis of TCS-induced immunotoxicity through m 6 A-RNA epigenetic modification and aid in assessing its ecological risks, informing strategies for disease prevention linked to environmental contaminants.
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Journal of Zhejiang University-SCIENCE B
·
2017
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IF 1.676
Ju-qing Huang,Rui-ting Qi,Mei-rong Pang,Cong Liu,Guang-yu Li,Ying Zhang
Bamboo shavings, the outer or intermediate layer of bamboo stems, are the bulk of by-products produced in bamboo processing. In this study we investigated the isolation, chemical characterization, and immunostimulatory activity in vitro of the hemicelluloses from bamboo shavings. Shavings were first pretreated by steam explosion. The optimal pretreatment was found to be steam explosion at 2.2 MPa for 1 min. Following this pretreatment, the yield of hemicelluloses reached (2.05±0.22)% (based on the dry dewaxed raw materials), which was 5.7-fold higher than that of untreated samples. Bamboo-shavings hemicellulose (BSH) was then prepared by hot water extraction and ethanol precipitation from the steam-exploded shavings. Purification of BSH by anion-exchange chromatography of diethylaminoethanol (DEAE)-sepharose Fast Flow resulted in a neutral fraction (BSH-1, purity of 95.3%, yield of 1.06%) and an acidic fraction (BSH-2, purity of 92.5%, yield of 0.79%). The weight-average molecular weights ( M w ) of BSH-1 and BSH-2 were 12 800 and 11 300 g/mol, respectively. Chemical and structural analyses by Fourier transform infrared spectroscopy (FT-IR), 1D ( 1 H and 13 C) and 2D (heteronuclear single quantum correlation (HSQC)) nuclear magnetic resonance (NMR) spectra revealed that BSH-1 was O -acetylated-arabinoxylan and BSH-2 was O -acetylated-(4- O -methylglucurono)-arabinoxylan. BSH-1 had a higher content of acetyl groups than BSH-2. For the immunomodulatory activity in vitro, BSH and BSH-2 significantly stimulated mouse splenocyte proliferation while BSH-1 had no effect; BSH, BSH-1, and BSH-2 markedly enhanced the phagocytosis activity and nitric oxide production of the murine macrophage RAW264.7 in a dose-dependent manner. Our results suggest that the water-extractable hemicelluloses from steam-exploded bamboo shavings are naturally acetylated and have immunostimulatory activity.
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Journal of Functional Foods
·
2024
·
IF 3.8
Qian Lei,Yaoyu Zhang,Tongtong Yang,Lei Chen,Xinxin Pei,Yujie Zhang,Tengfei Ma,Yuzhu Xie,Yi Wang,Hongxia Li,Cheng Cao,Buchang Zhang,Changzhi Xu
Listeria monocytogenes infection often leads to listeriosis in immunocompromised individuals, and screening for potential nutrients that improve immunity may be beneficial. Here, we found that pre-incubation with heptadecanoic acid (C17:0), an odd-chain saturated fatty acid, significantly inhibited L.monocytogenes replication in differentiated THP-1 and RAW264.7, as evidenced by comparison of listeriolysin expression and colony count. Mechanistically, the administration of C17:0 triggered the activation of the IFN and NF-κB signaling pathways. Furthermore, C17:0 upregulated ACOD1 expression and the generation of itaconate, an anti-bacterial metabolite in macrophages. Based on these results, our comparative analysis showed that sheep tail fat (STF), ruminant lipid rich in C17:0, had similar anti- L.monocytogenes effect. Collectively, our data highlighted the function of C17:0 in boosting the immune response, shedding light on the application of C17:0 and STF as potential candidates for strengthening anti- L.monocytogenes response.
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CHEMOSPHERE
·
2024
·
IF 8.1
Shizong Wang,Jianlong Wang
Electron transfer played key role in peroxymonosulfate (PMS) activation for heterogeneous Fenton-like catalysts (HFCs). However, the relationship between electron exchange capacity (EEC) and catalytic activity of HFCs has not been elucidated. Herein, thirteen HFCs reported in our previous studies were selected to measure their EEC via electrochemical methods and to investigate the correlation between EEC and catalytic activity for PMS. The results show that nitrogen-doped graphene oxide had much higher EEC (5.299 mM(e) g −1 ), followed by reduced graphene oxide (3.23 mM(e) g −1 ), nitrogen-doped biochar-700 (2.032 mM(e) g −1 ), graphene oxdie (1.789 mM(e) g −1 ), nitrogen-doped biochar-300 (1.15 mM(e) g −1 ), g-C 3 N 4 (0.752 mM(e) g −1 ) and biochar (0.351 mM(e) g −1 ). For carbon materials, their catalytic activity was not determined by electron donor capacity (EDC), electron acceptor capacity (EAC) and EEC (EDC + EAC), but was linear correlation with |EDC-EAC| that can characterize the extent of HFCs reacting with PMS. The higher the |EDC-EAC| is, the higher the catalytic activity of HFCs is. For carbonaceous materials, their catalytic activity was not proportional to EAC, but had good linear correlation with EDC and |EDC-EAC|. The discrepancy between carbon materials and carbonaceous materials could be due to the different activation mechanisms. Further analysis found that there was no correlation between EEC and the reactive species derived from PMS, indicating that the produced reactive species was not only controlled by EEC. This study firstly elucidated the correlation between EEC and catalytic activity of HFCs, and |EDC-EAC| could be used as an index for evaluating the catalytic activity of HFCs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jianming Wu,Shuai Chen,Qinjie Xu,Qingkai Pang,Peng Li,Yulin Li
In this paper, a novel programable sewage-cleaning technology for the regeneration of antibacterial nanocomposites via the removal of wastewater pollutants is presented. Montmorillonite (MMT) was encapsulated in poly(vinyl alcohol) (PVA)-enhanced chitosan (CTS) hydrogels to form MMT-loaded nanocomposite biofilms (PCM). The PCM nanocomposite biofilms exhibited increased breaking strength and elongation at break, by factors of approximately 1.38 and 1.40, respectively, compared with those of the pure PVA/CTS biofilms. The maximum adsorption capacity of the PCM nanocomposite biofilms toward tetracycline and Ag(I) is 275.0 and 567.0 mg/g, respectively. The adsorbed nanocomposite biofilms exhibited excellent antibacterial properties against Staphylococcus aureus and Escherichia coli . Meanwhile, the nanocomposite also showed an effective adsorption capacity toward other toxic components, where the highest adsorption capacity is 2748.0 mg/g (for methyl blue). The simulation results indicated that the adsorption behaviors of the malachite green, neutral red, methyl blue, tetracycline, Cu(II), Zn(II), and Ag(I) by the PCM nanocomposite biofilms followed pseudo-second-order kinetic and Freundlich isotherm models. Furthermore, the PCM nanocomposite biofilms are stable in PBS solution but degradable in lysozyme-containing PBS solution, suggesting their potential application in the wastewater treatment as well as antibacterial fields.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Tianyi Hu,Qun Liu,Zeng Xu,Zhengjiang Zhu,Wuyin Weng,Yucang Zhang
Starch/cellulose-based materials represent one of the most promising classes of biodegradable biomass substrates. However, their susceptibility to microbial contamination and limited function limit their applications in modern intelligent food packaging. In this study, cinnamaldehyde (CA) was employed as an antimicrobial agent, while the food-safe synthetic dyes neutral red (NR) and bromothymol blue (BTB) served as pH-responsive indicators. Using a one-pot strategy, starch granules were simultaneously gelatinized and encapsulated CA, while co-blending with bacterial cellulose nanofibers (CNFs) to yield a uniform film-forming solution. Upon incorporation of NR and BTB into the film solution, the resulting CA/HAS-CNFs-(NR&BTB) film exhibited excellent mechanical performance (tensile strength: 10.20 MPa; elongation at break: 14.76 %) owing to hydrogen bond reorganization between CA/HAS inclusion complexes and CNFs. FTIR and CLSM analyses confirmed the successful encapsulation of CA and uniform dispersion within the matrix. The film demonstrated distinct and reversible color transitions in response to pH variation and effectively tracked shrimp freshness, with a visible change from orange to yellow-green over time during storage. The R, G and B values of the film can be directly obtained through the mobile phone app, and then the GCS value can be calculated to obtain the freshness status of the food. Biodegradation studies under simulated microplastic-contaminated environments revealed that the composite film had favorable environmental compatibility. This work provides a sustainable and intelligent packaging solution with significant potential for real-time food freshness monitoring.
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ENVIRONMENTAL HEALTH PERSPECTIVES
·
2025
·Vol. 133
·期 5
·057007
·
IF 9.8
Jie Song,Qian Pu,Chen Chen,Xingcheng Liu,Xinlei Zhang,Zejun Wang,Jin Yan,Xuedong Wang,Huili Wang,Qiuhui Qian
Background: Micro/nanoplastics and silver nanoparticles (AgNPs) are emerging environmental contaminants widely detected in aquatic environments. However, previous research has primarily focused on the interactions between micro/nanoplastics and organic substances or heavy metals, whereas the interactions and combined toxic effects of micro/nanoplastics with AgNPs remain unclear. Objective: Our study aimed to investigate the effects and mechanisms of coexposure to AgNPs and polystyrene micro/nanospheres (PS M/NPs) on the nervous system, comparing the toxicity of AgNPs alone and in combination with PS M/NPs in larval zebrafish. Methods: We investigated the dynamics of AgNPs’ ( 5 nm ) adsorption onto PS M/NPs ( 5 μ m / 100 nm ) using inductively coupled plasma–mass spectrometry. Zebrafish larvae were coexposed to PS M/NPs ( 200 μ g / L ) and AgNPs ( 10 μ g / L ) from 6 h post fertilization (hpf) to 72 hpf to ∼ 120 hpf to evaluate neuroinflammatory effects from multiple perspectives, including developmental abnormalities, oxidative stress, neurobehavioral differences, vascular development, immune responses, differences in gene expression, and differences upon neuroinflammation inhibitor addition. Results: Adsorption experiments showed PS M/NPs could stably adsorb AgNPs, with higher adsorption in smaller particles. Zebrafish larvae exposed to combined PS M/NPs and AgNPs demonstrated neurodevelopmental abnormalities, including developmental malformations, lower levels of locomotor activity, delayed response, and abnormal neuronal development. In addition, exposed zebrafish also exhibited disrupted neurodevelopmental markers, including vascular and apoptotic indicators, and oxidative stress and neuroimmune responses. Quantitative real-time polymerase chain reaction analysis showed differences in gene expression within neurotoxic pathways in PS M/NPs and AgNPs-exposed zebrafish, focusing on key genes in immunity, apoptosis, vascular, and neural development. Furthermore, these neurotoxic effects induced by combined exposure were alleviated following the introduction of the neuroinflammation inhibitor curcumin. Discussion: Our findings demonstrate that polystyrene nanospheres (PSNPs) intensified AgNPs-induced neurotoxicity in larval zebrafish, whereas polystyrene microspheres (PSMPs) had a lesser effect, indicating distinct gene regulation roles when combined with AgNPs. These findings enhance the assessment of environmental risks in settings with coexisting nanomaterials and microplastics, offering important insights for evaluating combined exposure risks. https://doi.org/10.1289/EHP14873
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DYES AND PIGMENTS
·
2025
·
IF 4.2
Zhaokang Zheng,Zhen Liu,Yujie Ma,Aiwu Wang,Cangtao Zhou,Muhammad Humayun,Mohamed Bououdina
Oxygen vacancies (OVs) govern pollutant adsorption, but quantifying their impact remains challenging. Herein, oxygen-vacancy-rich amorphous MoO 3-x nanostructures (A-MoO 3-x ) were fabricated through a simple one-step hydrothermal route. Compared with previously reported MoO 3 -based adsorbents, A-MoO 3-x exhibits higher adsorption capacity. The Liu model yields a maximum methylene-blue (MB) uptake of 1549.8 mg/g, kinetics obeys a pseudo-second-order model, and thermodynamic analysis confirms a spontaneous, exothermic process. Moreover, A-MoO 3-x selectively removes cationic dyes within a competitive six-dye system (four cationic and two anionic dyes). XPS, EPR and FTIR reveal that cationic dyes are captured via a dual mechanism of electrostatic attraction and electron transfer. When OVs density is deliberately reduced to 76 % of its original value, the adsorption capacity decreases by 66 %, directly evidencing OVs as the dominant active sites. This work elucidates the decisive role of OVs in modulating the adsorption performance of metal oxides.
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Frontiers in Immunology
·
2025
·
IF 5.9
DongXue Ma,YuXin Zhang,Jia Zhang,Jun Shi,ShanHu Gao,Fei Long,Xin Wang,XingYu Pu,Jiayao Sun,Shuang Liang,Richard D. Cannon,Silas Villas-Boas,Ting-Li Han
Introduction: Escherichia coli Nissle 1917 (EcN) is one of the most extensively studied nonpathogenic Gram-negative probiotic strains worldwide. Recent research has highlighted the ability of EcN outer membrane vesicles (OMVs) to enhance the phagocytosis and proliferation of RAW264.7 macrophages. However, the impact of EcN-OMVs on M1/M2 polarization and metabolic modulation remains unknown.Methods: In this study, we evaluated the metabolic effects of EcN-OMVs on RAW264.7 macrophage polarization using metabolomic, transcriptomic, and fluxomic approaches.Reuslts: We found that the RAW264.7 macrophages phagocytosed EcN-OMVs, triggering upregulation of the HIF-1, mTORC1, and NF-κB signaling pathways. This metabolic reprogramming enhanced glycolysis, suppressed the TCA cycle, elevated intracellular reactive oxygen species (ROS), TNF-α, IL-6, IL-1β, ATP, and nitric oxide (NO) production, and promoted macrophage proliferation, migration, invasion, and M1-type polarization.Discussion: In summary, this research establishes a theoretical foundation for utilizing probiotic OMVs in immunomodulatory therapeutic applications.
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