Water
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2024
·Vol. 16
·期 18
·2665
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IF 3
Huifang Sun,Yanjun Liu,Yifan Zhou,Zuliang Chen,Jianfeng Li
This study investigates the synthesis of iron-based nanoparticles (Fe NPs) usingpomegranateleaf extracts and their application in removing indole, a persistent organic pollutant commonly found in wastewater. The physicochemical properties of the synthesized Fe NPs and the active biomolecules in thepomegranateleaf extracts were comprehensively characterized. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) analyses revealed that the Fe NPs exhibited quasi-spherical shapes, with sizes ranging from 75 to 105 nm. Energy-Dispersive X-ray Spectroscopy (EDS) confirmed a homogeneous distribution of elements, including C, O, Fe, and S, on the nanoparticle surfaces, with weight percentages of 43.59%, 42.95%, 12.58%, and 0.88%, respectively. Fourier-transform infrared spectroscopy (FTIR) identified key functional groups like −OH, −COOH, and −C=O, which are essential for the capping and stabilization of the nanoparticles. Biomolecules such as ellagic acid (C14H6O8) and gallic acid (C7H6O5) functioned as reducing agents, improving nanoparticle dispersion and preventing aggregation. The synthesized Fe NPs quickly achieved 45.5% removal of indole within just 20 min and maintained a stable removal efficiency of approximately 51.4% after 90 min. This performance was attributed to the synergetic interaction between the biomolecules and the nanoparticles, with the monolayer adsorption of indole molecules on the Fe NP surfaces likely setting an upper limit on the maximum achievable removal efficiency. It appears from this study thatpomegranateleaf extracts can be effectively utilized to synthesize Fe NPs as a novel and eco-friendly approach, demonstrating promising potential for the rapid removal of indole from aqueous solutions.
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MOLECULAR MEDICINE
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2025
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IF 6.4
Yufei Deng,Xiaoying Hou,Qian Fang,Haiping Wang,Xiaoxuan Li,Zhiyong Hu,Zhaolu Liu,Limei Fan,Yunyi Liu,Zhengqi Fu,Xiji Shu,Binlian Sun,Lijun Huang,Yuchen Liu
Background FOLFOX is the recommended chemotherapy regimen for colorectal cancer (CRC), but its response rate remains low. Our previous studies have established a close relationship between gut microbiota and the anti-CRC effect of FOLFOX, though the underlying mechanisms remain unclear. Diet has been confirmed as a key factor influencing gut microbiota, and high-salt diets, representative of western dietary habits, has been shown to affect gut microbiota, immune function, and the risk of developing CRC. However, the impact of high-salt diets on the anti-CRC efficacy of FOLFOX remains unstudied. Therefore, we aimed to investigate the effect and mechanism of high-salt diets on the anti-CRC effect of FOLFOX. Methods We performed 16 S rRNA sequencing and T500 targeted metabolomics analysis on fecal samples from CRC patients and healthy adults. A CRC orthotopic xenograft mouse model was used to study the effect of a high-salt diet on FOLFOX’s anti-CRC efficacy. 16 S rRNA sequencing and non-targeted metabolomics were conducted on mouse fecal samples. Flow cytometry was used to assess immune cell infiltration in tumor and paracancerous tissues. A mouse macrophage conditioned medium system, with tryptophan metabolites, was employed to annotate the functional metabolites, followed by in vivo verification using the orthotopic xenograft mouse model. Results The structure and metabolic profiles of gut microbiota are significantly different between 9 healthy adults and 6 CRC patients. A high-salt diet significantly reduced the efficacy of FOLFOX in mice, with notable changes in gut microbiota and related metabolites. Correlation analysis revealed a significant relationship between gut microbiota, tryptophan metabolites and FOLFOX efficacy. Flow cytometry indicated that a high-salt diet altered macrophage infiltration (CD45 + F4/80 + ) in both the tumor and paracancerous tissues. In vitro experiments confirmed that the tryptophan metabolite SK reduced FOLFOX efficacy, while IPA enhanced it through macrophage-conditioned medium. In vivo, we verified that under a high-salt diet, SK inhibited the efficacy of FOLFOX, while IPA promoted it. Conclusion A high-salt diet reduces the anti-CRC efficacy of FOLFOX through gut bacterial tryptophan metabolism mediated macrophage immunomodulation. Supplementary Information The online version contains supplementary material available at 10.1186/s10020-025-01122-8.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
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IF 8.2
Yanjuan Gao,Zhihong Liu,Shaobo Wang,Aijuan Zhou,Xvfeng Lv,Xiuping Yue
Indole and pyridine, which are highly produced refractory compounds in the industrial wastewater, exhibit poor degradation capabilities in natural environments. In this study, we developed an anaerobic digestion system coupled with weak electric mediation (ED), and investigated the promoting effect of weak electricity on indole and pyridine biodegradation. The degradation characteristics were systematically explored, and the results showed that the degradation rate and mineralization of indole and pyridine were significantly enhanced, the production of CH 4 was increased 1.4-fold, and the optimal voltages were 1.0 V and 0.8 V in the ED, respectively. Moreover, simultaneous removal of carbon and nitrogen was achieved. Gas chromatography–mass spectrometry analysis verified the transformation products, and possible pathways were proposed. Several byproducts of indole and pyridine were identified, with oxindole and glutaric dialdehyde being the main metabolites, respectively. Additionally, density functional theory (DFT) analysis was performed to investigated the radical indices and stabilities of the molecules to further confirm the degradation pathway. Microbial structure analysis demonstrated that the electrically mediated enhanced metabolism and activity of functional microbes, led to the promotion of indole and pyridine mineralization. Moreover, such species as degrading bacteria ( Alicycliphilus , Shinella ) and electroactive bacteria ( Achromobacter ), anaerobic ammonia-oxidizing bacteria ( SM1A02 ), and denitrifying bacteria ( Thiobacillus ) coexisted. This study demonstrates that weak electric mediation is a promising methodology for enhancing the removal of indole and pyridine from wastewater under anaerobic conditions.
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ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Chun-Yan Liu,Yun Chen,Jun Hu
The identification of the electrogenerated reactive intermediates is essential for an in-depth understanding of the electroredox processes. Although various short-lived intermediates are well characterized by coupling electrochemistry with mass spectrometry (EC/MS), many electrogenerated transient species (τ < 1 μs) are still rarely captured by the currently available EC/MS approaches. Here, we present a low-delay coupling device, which was constructed by decorating a microelectrode into the front tip of a microsized ion emitter. For the first time, the in situ detection of a previously hidden intermediate, i.e., the transient nitrenium ion of carbazole (τ = 333 ns), was achieved. The electrochemical generation of indole nitrenium ion, whose half-life is estimated to be shorter compared to the carbazole nitrenium ion due to less resonance stabilization, was also confirmed by direct observation. This clog-free microelectrode/ion emitter is cheap and easy to fabricate and offers a general and powerful approach to monitoring the fast reactions of electrogenerated reactive intermediates. We believe that our integrated EC/MS approach holds substantial potential for broad applicability, particularly in probing the intricate and ultrafast electroredox processes occurring at the electrode–solution interface.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
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IF 6.2
Yuqi Zhu,Xuna Tao,Tongtong Yan,Shuyuan Cao,Ping Jiang,Zhan Zhang,Lei Li,Qian Wu
Objective This study aimed to investigate the mechanism that Lactobacillus murinu s ( L. murinu s) alleviated lung inflammation induced by polycyclic aromatic hydrocarbons (PAHs) exposure based on metabolomics. Methods Female mice were administrated with PAHs mix, L. murinus and indoleacrylic acid (IA) or indolealdehyde (IAId). Microbial diversity in feces was detected by 16 S rRNA gene sequencing. Non-targeted metabolomics analysis in urine samples and targeted analysis of tryptophan metabolites in serum by UPLC-Orbitrap-MS and short-chain fatty acids (SCFA) in feces by GC-MS were performed, respectively. Flow cytometry was used to determine T helper immune cell differentiation in gut and lung tissues. The levels of IgE, IL-4 and IL-17A in the bronchoalveolar lavage fluid (BALF) or serum were detected by ELISA. The expressions of aryl hydrocarbon receptor ( Ahr ), cyto chrome P45 0 1A1 ( Cyp1a1 ) and forkheadbox protein 3 ( Foxp3 ) genes and the histone deacetylation activity were detected by qPCR and by ELISA in lung tissues, respectively. Results PAHs exposure induced lung inflammation and microbial composition shifts and tryptophan metabolism disturbance in mice. L. murinus alleviated PAHs-induced lung inflammation and inhibited T helper cell 17 (Th17) cell differentiation and promoted regulatory T cells (Treg) cell differentiation. L. murinus increased the levels of IA and IAId in the serum and regulated Th17/Treg imbalance by activating AhR. Additionally, L. murinus restored PAHs-induced decrease of butyric acid and valeric acid which can reduce the histone deacetylase (HDAC) level in the lung tissues, enhancing the expression of the Foxp3 gene and promoting Treg cell differentiation. Conclusion our study illustrated that L. murinus alleviated PAHs-induced lung inflammation and regulated Th17/Treg cell differentiation by regulating host tryptophan metabolism and SCFA levels. The study provided new insights into the reciprocal influence between gut microbiota, host metabolism and the immune system, suggesting that L. murinus might have the potential as a novel therapeutic strategy for lung diseases caused by environmental pollution in the future.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
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IF 6.3
Yaoyang Xia,Hao Xiang,Fu Wan,Ruiqi Liu,Kaida Hu,Yujie Zheng,Quan Zhou,Weigen Chen
Aqueous zinc ion batteries (AZIBs) with MnO 2 as the positive electrode have attracted extensive attention due to their high theoretical energy density. However, the dissolution of the positive electrode and the poor conductivity during the cycling process affect its development prospects. In this paper, δ-MnO 2 with larger interlayer spacing is adopted, and pre-intercalation of Ni 2+ is used to further expand its interlayer spacing to enhance the diffusion kinetics of Zn 2+ in the cathode. Density functional theory (DFT) calculations show that the charge distribution around Mn and O atoms will be changed to improve the conductivity of the MnO 2 cathode by the Ni 2+ intercalation. However, the intercalation of metal cations will lead to the production of more Mn 3+ . which will lead to further manganese dissolution. Therefore, we prepared a polyindole (PIn) coating layer with high conductivity and can effectively prevent cathode erosion by in-situ polymerization. The formation of a heterogeneous interface between PIn and MnO 2 surface not only provides more active electrochemical sites, but also enhances the conductivity of the cathode and reduces the occurrence of manganese dissolution, which greatly improves the cycle performance and specific capacity of the cathode. Ni-MnO 2 @PIn delivers a discharge capacity of 385 mAh g −1 at a current density of 0.1 A g −1 , and maintains a capacity of 278 mAh g −1 after 200 cycles at a current density of 0.3 A g −1 , with a capacity retention of 95 %.
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Frontiers in Cellular and Infection Microbiology
·
2025
·
IF 4.8
Xueyao Fang,Yanhui Chen,Longhua Hu,Shumin Gu,Junqi Zhu,Yaping Hang,Xingwei Cao,Yanping Xiao,Hong Luo,Chuwen Zhao,Lianhua Xiao,Qiaoshi Zhong
Indole, a crucial bacterial signaling molecule, plays a fundamental role in regulating various physiological processes within bacteria, including growth, acid tolerance, biofilm development, motility, and other cellular functions. Its regulatory influence extends beyond indole-producing bacteria, significantly impacting the physiological activities in non-indole-producing species. In this study, we demonstrate that indole enhances the pathogenicity and viability of Klebsiella pneumoniae using the Galleria mellonella infection model and serum killing assay. Concurrently, indole has varying effects on biofilm formation in K. pneumoniae, with some strains showing enhanced biofilm formation ability. To elucidate the underlying molecular mechanisms, transcriptome analysis revealed that indole exposure in K. pneumoniae led to the upregulation of genes associated with pili formation and iron acquisition systems, while simultaneously inducing oxidative stress responses. Additionally, our analysis uncovered extensive metabolic remodeling. Specifically, we observed significant upregulation of genes involved in simple carbohydrate utilization pathways, including those responsible for galactose, mannose, and maltose metabolism, as well as enhanced expression of genes associated with pyrimidine biosynthesis. These findings collectively indicate that indole enhances the intestinal colonization and pathogenicity of K. pneumoniae primarily by modulation of fimbriae expression and metabolic pathway regulation.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2024
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IF 6.1
Siyue Liu,Jinge Xie,Wenqi Luan,Chen Liu,Xiwen Chen,Defu Chen
The current trend in agricultural development is the establishment of sustainable agricultural systems. This involves utilizing and implementing eco-friendly biofertilizers and biocontrol agents as alternatives to conventional fertilizers and pesticides. A plant growth-promoting fungal strain, that could alter root system architecture and promote the growth of Arabidopsis seedlings in a non-contact manner by releasing volatile organic compounds (VOCs) was isolated in this study. 26S rDNA sequencing revealed that the strain was a yeast-like fungus, Papiliotrema flavescens . Analysis of plant growth-promoting traits revealed that the fungus could produce indole-3-acetic acid and ammonia and fix nitrogen. Transcriptome analysis in combination with inhibitor experiments revealed that P . flavescens VOCs triggered metabolic alterations, promoted auxin accumulation and distribution in the roots, and coordinated ethylene signaling, thus inhibiting primary root elongation and inducing lateral root formation in Arabidopsis . Additionally, transcriptome analysis and fungal infection experiments confirmed that pretreatment with P . flavescens stimulated the defense response of Arabidopsis to boost its resistance to the pathogenic fungus Botrytis cinerea . Solid-phase microextraction, which was followed by gas chromatography-mass spectrometry analysis, identified three VOCs (acetoin, naphthalene and indole) with significant plant growth-promoting attributes. Their roles were confirmed using further pharmacological experiments and upregulated expression of auxin- and ethylene-related genes. Our study serves as an essential reference for utilizing P . flavescens as a potential biological fertilizer and biocontrol agent.
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OPTICAL MATERIALS
·
2025
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IF 4.2
Lirong Chen,Mengyang Lv,Yanyu Wan,Yu Sun,Zhenchao Gu,Shuaishuai Zhang
g-C 3 N 4 /TiO 2 photocatalysts were in-situ synthesized via a straightforward high-temperature calcination method and used to activate persulfate (PS) to degrade indole under different light irradiation. The prepared g-C 3 N 4 /TiO 2 photocatalysts were characterized by SEM, TEM, XRD, BET, XPS, FTIR, and photocurrent response analysis. The CNT 1:5 (mass ratio of g-C 3 N 4 to TiO 2 of 1:5) and CNT 24:1 (mass ratio of g-C 3 N 4 to TiO 2 of 24:1) showed the best performance in degradation of indole solution under UV and visible light, respectively. Free radical quenching experiments and band structure analysis determined that the UV/CNT 1:5 and PS-Vis/CNT 24:1 systems followed an S-scheme and type-II transfer mechanism, respectively. A detailed analysis using GC-MS revealed the indole's intermediate products and proposed the indole's degradation pathway. Furthermore, using natural water matrices, the removal of other typical organic compounds, including quinoline, pyridine, and phenol, was investigated in the UV/CNT 1:5 and PS-Vis/CNT 24:1 systems. The results of this study promote the application of g-C 3 N 4 /TiO 2 composites as organic degradation catalysts, reflecting the potential of composite materials in the removal of toxic organic compounds in coal chemical wastewater.
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CHEMBIOCHEM
·
2025
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IF 2.8
Shiqi Luo,Wei Song,Guipeng Hu,Jian Wen,Xiaomin Li,Cong Gao,Jia Liu,Wanqing Wei,Liming Liu
Graphical Here, we have constructed a dual-enzyme ( Ec TnaA, Ma FMO) cascade pathway for the biosynthesis of indigo from L-tryptophan and enhanced the enzymatic activity of Ma FMO through mechanism-guided rational design. Subsequently, by optimizing promoters and increasing copy numbers to regulate the expression levels of Ec TnaA and Ma FMO D197E , under optimal conditions, E. coli IND-13 achieved the highest yield to date using glucose as the substrate. Indigo is widely used in dyes, medicines and semiconductors materials due to its excellent dyeing efficiency, antibacterial, antiviral, anticancer, anti-corrosion, and thermostability properties. Here, a biosynthetic pathway for indigo was designed, integrating two enzymes ( Ec TnaA, Ma FMO) into a higher L-tryptophan-producing the strain Escherichia coli TRP. However, the lower catalytic activity of Ma FMO was a bottleneck for increasing indigo titers. To overcome this limitation, the enzyme activity of Ma FMO was enhanced through mechanism-guided rational design. The optimal mutant obtained in this study, Ma FMO D197E , whose k cat / K m was 1.34 times that of the wild type, and its specific activity was 2.36 times that of the wild type. In addition, the expression levels of Ec TnaA and Ma FMO D197E were regulated by optimizing the promoters and increasing the copy number to generate the strain E. coli IND-13. Finally, in the optimal fermentation conditions (220 rpm, 0.05 % Tween-80), the strain E. coli IND-13 achieved the indigo titer of 568.52 mg/L in a 5-L bioreactor, with the yield and productivity of 2.62 mg/g and 12.96 mg/L/h (the highest to date), respectively. The results presented here can lay a foundation for further construction of cell factories for indigo and its derivatives with industrial application potential.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2024
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IF 20.2
Qingqing Li,Zhiwei Sun,Yilin Wei,Zixu Ma,Liucheng Wang,Renfeng Nie
Reversible hydrogen storage via Liquid organic hydrogen carriers (LOHCs) is one of the key technologies for efficient utilization of hydrogen energy. How to construct efficient dehydrogenation/hydrogenation catalysts that enable low-temperature catalysis still remains challenging. Herein, we present the design and construction of single-atom Co-N-C(x) catalysts allowing significantly improved acceptorless dehydrogenation of 1,2,3,4-tetrahydroquinoline (THQ). By varying the ligand/Co ratio, the optimal Co-N-C(1.5) can achieve 97.5% dehydrogenated product and 0.82 h −1 TOF value at ambient temperature, overperforming many reported non-noble metal even noble metal catalysts. This catalyst exhibits good recyclability and substrate versatility, enabling ambient temperature dehydrogenation of various N-heterocycles and efficient reverse hydrogenation of aromatic N-heterocycles, thus achieving the loop of reversible hydrogen storage. The active catalyst centers consist of specific Co-N x moieties and nitrogen species within the carbon support. Controlled experiments reveal that the different performance of Co-N-C(x) is determined by density of the Co-N x , and the rate-determining step of THQ dehydrogenation is correlated with the bond cleavages in surface-bound THQ. This work offers a promising pathway for energy-efficient and cost-effective hydrogen energy utilization under practical conditions.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2012
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IF 3.07
Peng Liao,Songhu Yuan,Wenjing Xie,Wenbiao Zhang,Man Tong,Kun Wang
The adsorption kinetics and thermodynamics of nitrogen-heterocyclic compounds (NHCs), pyridine, indole and quinoline, in aqueous solutions on bamboo charcoal (BC), as well as the regeneration of spent BC by microwave radiation, are investigated. BC is produced by incomplete combustion of moso bamboo at high temperature and nitrogen atmosphere. Adsorption kinetics is analyzed using pseudo-first-order and pseudo-second-order as well as Weber–Morris model. The results show that NHC adsorption on BC is predominantly regulated by surface diffusion in initial 1 h followed by intraparticle diffusion in later stage. BC exhibits a strong adsorption affinity to NHCs, and the adsorption isotherms are well described by Freundlich model. Thermodynamic analysis indicates that the adsorption is spontaneous and endothermic. Adsorption site energy analysis illustrates a distribution of adsorption energy, which indicates the heterogeneous sites on BC for NHC adsorption. Furthermore, spent BC with NHC adsorption can be effectively regenerated by MW radiation. The adsorption capacity becomes even higher than that of virgin BC after five times of adsorption–regeneration cycles. This study proves BC is a promising adsorbent for NHC removal in wastewater.
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