ACS Applied Materials & Interfaces
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2025
·
IF 8.2
Mohamed Bahri,Guoguang Rong,Xixi Song,Peiwu Qin,Mohamad Sawan
Signal amplification has become a paramount strategy in biosensing, offering substantial improvements in sensitivity and detection limits for analytical platforms. However, conventional methods often rely on separate amplification stages and intricate chemical functionalization, leading to inefficiencies and greater complexity in biosensing workflows. In this article, we introduce a novel dual-purpose application of AuNPs@CuMOF for both surface functionalization and signal amplification, enabling electrochemical biosensing of neuron-specific enolase (NSE), a key biological marker for neuronal disorders and small cell lung cancer. The proposed biosensing strategy implies immobilizing the biotinylated NSE antibody onto the AuNPs@CuMOF-functionalized surface of a screen-printed carbon electrode (SPCE), alongside a thiolated NSE aptamer conjugated to AuNPs@CuMOF for signal amplification. Following this approach, the proposed immunosensor showcased a detection limit (LOD) down to 0.02 pg/mL within the span of 0.1 pg/mL to 200 ng/mL, outperforming or matching previously reported NSE biosensors. Besides, the suggested NSE biosensor reliably identified NSE in spiked human serum samples, showing both strong selectivity and sensitivity, with performance metrics on par with those of standard ELISA tests, with the relative error ranging from 12.03% to 5.63%. By leveraging the combined advantages of CuMOF’s large surface area, versatility, and stability, the proposed method simplifies the biosensing workflow while enhancing sensitivity through intrinsic amplification, resulting in a robust and efficient platform for the detection of biomarkers. We highlight in this work the transformative potential of multifunctional nanomaterials in advancing electrochemical biosensing technologies, wihch represents a significant milestone in tumor diagnosis in clinical settings.
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BIOSENSORS & BIOELECTRONICS
·
2025
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IF 10.5
Zhenjie Zhang,Wei Wei,Anne Ailina Li,Chenhao Wang,Chengchen Tang,Qing Wang,Lianqun Zhou,Hui Kong
Here, we report a wearable device integrating a sweat collection unit and a molecularly imprinted polymer (MIP) - based sensor for the non-invasive detection of adrenaline (AD). In this paper, a new MIP method is proposed, which uses AD molecule as template, and electropolymerizes aniline and 3-aminophenylboronic acid (3-APBA) to form molecular imprinting. Surface modifications, incorporating carbon nanotubes (CNT) and MIP optimization, enhanced the device's specific surface area by 166.45% (± 2.82%), yielding a wide linear detection range of AD (10 -6 μmol L -1 to 10 3 μmol L -1 ). In this study, a three-dimensional response model was constructed by analyzing the current values corresponding to sweat pH levels (4.0-8.0) and AD concentrations, enabling a linear response and accurate prediction of AD levels in sweat. The integrated flexible electronic platform supports real-time data transmission via Bluetooth or NFC to a mobile application, facilitating efficient detection. Comprehensive integration and validation, including artificial sweat testing and correlation with ELISA results, underscore the sensor’s potential as a robust and versatile solution for wearable health monitoring.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-9
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IF 5.3
Yao Zimin,Zheng Guoliang,Zheng Yonggui,Wen Xiaokun,Hong Xia,Yan Jiayi,Liu Wen,Wang Jiwei,Wang Kexin,Cao Shuo
Biomolecular detection based on a single fluorescence signal response is often difficult to completely eliminate the influence from interfering factors on the assay results. In this work, a ratiometric fluorescence probe was designed based on a dual emission carbon dot (CD). It produces dual-color emission (400 nm blue emission band and 560 nm green emission band) under 360 nm excitation. The two emission bands are believed to be originated from the distinct luminescent mechanisms: the intrinsic emission of CDs and intramolecular charge transfer (ICT) process, respectively. The energy transfer between the dual emission leads to the differentiated responses towards sialic acid (SA). As the concentration of SA increases, the two emission bands of CDs dispersion exhibit opposite variation trends. The blue emission intensity rose slightly and green emission intensity descended. The ratios of emission band intensity at 400 nm and 560 nm are served as quantitative assay of SA. The limit of detection reached 1.15 μM. The ratiometric fluorescence probe also shows a favorable selectivity for SA detection. The self-calibration and mutual verification functions of the probes enable consistent emission ratios across different models of commercial spectrometers. It minimizes the interference from the environment and instrumentation, achieving accurate and highly versatile SA detection. The utility of the probe for SA detection was verified by analyzing human serum, showing the tremendous potential of dual emission CDs for accurate SA detection. Graphical
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JOURNAL OF MOLECULAR STRUCTURE
·
2025
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IF 4.7
Xiaoyu Shen,Yejian Mo,Jian Ren,Kexin Liu,Chunlin Deng
Hyperuricemia (HUA) is a metabolic disease caused by elevated uric acid levels, with xanthine oxidase (XOD) as the key enzyme in uric acid production. Current treatments primarily use XOD inhibitors (e.g., allopurinol and febuxostat), but their lack of antioxidant activity limits their ability to counteract oxidative stress in HUA. Gallic acid (GA), a polyphenol with antioxidant properties, weakly inhibits XOD but is insufficient alone. Transition metal ions like Cu²⁺ are potent reversible XOD inhibitors, yet excessive concentrations cause toxicity. The coordination reaction effectively reduces metal ion toxicity while partially preserving the inherent antioxidant properties of polyphenols. Using gallic acid (GA) as a ligand with Cu²⁺, we synthesized Cu-GA complex, which forms a stable coordination polymer that minimally releases free Cu²⁺ in water. Cu-GA demonstrated superior XOD inhibition compared to allopurinol (AP), GA, and Cu²⁺ alone, functioning as a reversible inhibitor to reduce uric acid levels. Additionally, it maintained significant antioxidant activity while showing substantially lower cytotoxicity in LO2 and AML12 cells than free Cu²⁺. In conclusion, for Cu-GA, the coordination reaction retained the inhibitory effect of Cu 2+ on XOD, while conferring some antioxidant effects, and also reduced the biotoxicity of Cu 2+ .
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LANGMUIR
·
2026
·
IF 3.9
Rong Qiang,Fangjie Ren,Yulong Shao,Lei Rong,Rui Hao,De Zhang,Na Xiao,Zheng Guo,Qing Miao,Yaning Zong
Demand for lightweight and efficient electromagnetic wave (EW) absorbers continues to increase with technological advances in highly integrated electronics and military applications. Nitrogen doping is known to improve dipole polarization, contributing to broadband microwave absorption. In this work, nitrogen-doped carbon nanosheets with unique accordion-like structures were successfully synthesized via direct carbothermal reduction of uric acid. These architectures exhibit augmented microwave absorption resulting from synergistic loss effects including interfacial and dipole polarization. Furthermore, nitrogen doping results in the formation of lattice defects and electronic transport in three-dimensional conductive networks; these features collectively contribute to optimizing impedance matching and increasing the microwave absorption characteristics of materials. The high-temperature pyrolysis directly influences the graphitization degree, thereby significantly improving the dielectric loss. Experimental results demonstrate that the N-ALC-800 materials, at a filler content of 20 wt %, exhibit outstanding microwave absorption performance with an effective absorption bandwidth of 6.0 GHz. Furthermore, computer simulation technology (CST) indicates that the radar cross-section (RCS) value can be effectively reduced to 34.7 dB·m2. This study offers new insights and practical guidance for designing lightweight and high-efficiency carbon-based microwave absorbers.
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MATERIALS LETTERS
·
2026
·
IF 2.6
Ziqi Xu,Yuanbang Zhao,Ruoyu Wang,Haitao Ma,Yunpeng Wang,Hanying Wang
Copper-based materials are attractive for non-enzymatic glucose sensing due to their high activity and low cost. However, conventional powder-based electrodes often suffer from high interfacial resistance and limited active sites. Herein, we develop a facile strategy combining electrodeposition and anodization to directly fabricate self-supporting porous Cu₂O electrodes on copper foil. This binder-free electrode exhibits outstanding electrocatalytic performance, achieving a sensitivity of 946.19 μA·mM −1 ·cm −2 and a detection limit of 1.01 μM. Notably, it shows an exceptionally wide three-stage linear range (0.02–5.71 mM), covering physiological glucose levels in tears, saliva, interstitial fluid, and blood. The sensor also demonstrates excellent anti-interference ability and reproducibility, offering a promising approach for practical glucose detection.
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TALANTA
·
2026
·
IF 6.7
Chao Lu,Xue Fan,Fengna Xi,Yucheng Zhou
Sensitive and parallel individual detection of multiple tumor biomarkers is critical for early diagnosis and therapeutic monitoring of pancreatic cancer. Herein, a ternary electrochemiluminescence (ECL) system was developed by employing nanochannel-confined cerium dioxide (CeO 2 ) nanozyme as co-reactant enhancers for the luminol-dissolved oxygen (DO) system, enabling parallel individual immunoassays of carcinoembryonic antigen (CEA) and carbohydrate antigen 199 (CA199). Vertically-ordered mesoporous silica film (VMSF) was rapidly grown on an indium tin oxide (ITO) electrode, forming nanochannel array with thickness of 97 nm and diameters of 2.7 nm. CeO 2 nanozyme was synthesized in situ within the VMSF nanochannels via electrochemical deposition. Transmission electron microscopy (TEM), high-angle annular dark-field scanning TEM (HAADF-STEM), and energy-dispersive X-ray spectroscopy (EDX) elemental mapping confirmed CeO 2 nanozyme was confined in nanochannels with high dispersion. CeO 2 nanozyme exhibited peroxidase-like catalytic activity and enhanced the ECL intensity of the luminol-DO system by 13.9-fold under near-neutral conditions through catalysis of both oxygen reduction and luminol oxidation. After derivatization of the VMSF outer surface with epoxy groups, antibodies specific to CEA or CA199 were covalently immobilized to construct two immunorecognition interfaces. Target binding formed antigen-antibody complexes, increasing interfacial resistance and hindering diffusion of the ECL emitters, enabling a signal-off immunoassay mode. The resulting immunosensors displayed wide detection linear ranges from 0.1 pg mL −1 to 100 ng mL −1 for CEA and 0.1 × 10 −3 mU mL −1 to 1.0 × 10 4 mU mL −1 for CA199, with limit of detection (LOD) of 1.3 fg mL −1 and 0.5 × 10 −4 mU mL −1 , respectively. Both immunosensors exhibited good selectivity, reproducibility, and storage stability.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
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IF 5.4
Zhuanzhuan Shi,Qianqian Fu,Jiatao Gu,Ruoyu Wei,Xiaoshuai Wu,Xiaokun Fu,Feng Wang,Ruizhi Zhu,Chunxian Guo,Chang Ming Li
Nannochloropsis Oceanica ( N. Oceanica ), widely distributed across global marine environments, is rich in proteins and amino acids, serving as an excellent source of nitrogen self-doped biomass carbon. Further modification and optimization of this microalga for constructing high-performance sensing platforms hold significant research and practical implications. This study strategically employs iron doping during low-temperature carbonization to modulate the structural and electronic properties of N. Oceanica -derived carbon. Results demonstrate that varying iron concentrations greatly accelerates graphitization, tailors the pore architecture, and significantly increases the specific surface area, thereby creating abundant catalytic active sites. The optimized iron-doped carbon material serves as a highly effective electrocatalyst when integrating into silk fabric-based screen-printed electrodes. These biosensors achieve exceptional dopamine (DA) detection performance, featuring a remarkably low detection limit (3 nM) and a broad linear range (0.01–2000 µM). Crucially, mechanistic analysis reveals that iron facilitates electrocatalytic activity and significantly enhance charge-transfer efficiency, thereby significantly boosting catalytic ability. This work establishes a novel, low-temperature iron-doping strategy for transforming biomass carbon into a cost-effective, eco-friendly, and high-performance platform for flexible electrochemical DA biosensors, paving a way for advanced bioanalytical applications.
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ANALYST
·
2026
·
IF 3.3
Xueying Zhu,Shuo Tian,Shuyun Chen,Yunsen Wang,Dianping Tang
As a key alkaloid, the concentration of xanthine in the body often reflects various physiological or pathological states of the human body. However, conventional methods for xanthine detection often suffer from time-consuming procedures and a narrow linear range. The present study screened 30 nanozymes with varying Fe/S molar ratios using a hydrogen peroxide-3,3',5,5'-tetramethylbenzidine (H2O2-TMB) colorimetric system and identified FeS-loaded nitrogen-doped carbon nanozyme (Fe300S5-NC) as a highly efficient catalyst. Inspired by the biological metabolic pathway of xanthine, an XOD/Fe300S5-NC/TMB colorimetric system was constructed for the detection of xanthine. The constructed colorimetric sensing platform demonstrated facile operation and rapid response, with a linear xanthine detection range of 10–1000 μM and a detection limit of 0.97 μM. This work provides a viable strategy for designing artificial enzymes with high peroxidase-like activity, indicating potential for biosensing applications.
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ANALYTICAL SCIENCES
·
2026
·
IF 2
Bai Yu,Wang Jine,Han Shuang,Zhang Zhichao,Zhao Yuewu
Metal–organic frameworks (MOFs) have shown major promise for electrochemical dopamine (DA) sensing owing to their high surface area, tunable pores, and abundant active sites. In this work, iron-tetra(4-carboxyphenyl)porphyrin-coordinated assembly metal–organic framework (Fe-TCPP MOF material) capable of effectively catalyzing the oxidation of DA was synthesized. Subsequently, it was combined with reduced graphene oxide (rGO) with excellent electrical conductivity and perfluorosulfonic acid polymer (Nafion) with cation selectivity to construct an electrochemical sensor that could synergistically enhance the sensitivity of DA detection. The resulting Fe-TCPP/rGO/Nafion electrode detected DA from 0.1 to 3200 μM with a detection limit of 0.021 μM, outperforming most reported sensors. It also maintained high stability, selectivity, and interference resistance over repeated cycles, and achieved recoveries of 84.84–110.76% in real samples. Mechanistically, the porous Fe-TCPP MOF increased accessibility of catalytic sites; rGO improved charge transport and π–π adsorption; and Nafion provided cation-exchange selectivity and electrostatic preconcentration. These complementary roles yielded synergistic gains in catalytic oxidation, preconcentration, selectivity, and anti-interference. The integrated strategy offered a practical route to high-performance MOF-based DA sensors and a useful reference for developing efficient analytical materials. Graphical abstract
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 4
·233
·
IF 5.4
Gao Xiang,Hou Yujiao,Lin Fang,Wen Limei,Guo Yali,Kong Weijun,Hu Junping,Yang Jianhua
An electrochemical (EC) molecularly imprinted polymer (MIP) sensor for therapeutic drug monitoring of olanzapine (OLZ) has been developed. A “rime-like” gold-platinum nanostructured film (Au-Pt NF) was fabricated on a screen-printed carbon electrode (SPCE) via an one-step hydrogen bubble dynamic template (HBDT) method, followed by electropolymerization of a polyaniline-based MIP. The Au-Pt NF/SPCE served as an ideal three-dimensional conductive substrate with a large active area and enhanced electron-transfer capability, endowing the specific MIP sensor with high sensitivity. Using [Fe(CN) 6 ] 3−/4− as a redox probe, the sensor quantified OLZ by the decrease in differential pulse voltammetry current caused by target binding. It exhibited two linear ranges (0.005–0.4 µM and 0.4–10 µM) with a detection limit of 0.94 nM, and demonstrated high selectivity, reproducibility, and stability. The sensor achieved recoveries of 96.7–102.7% for OLZ in tablets, urine, and whole blood. This work provides a reliable, all-EC sensing platform with strong potential for rapid OLZ monitoring in clinical settings.
Section
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Xiaoting Zhao,Jiayi Lu,Wenzhong Niu,Qidong Wang,Yanxia Qi,Lu Shi,Yuchun Qiao,Jizhen Shang,Limin Lu,Shuai Li,Hua Wang
Flower-like copper nanocomposites (Cu NFs) were synthesized through the coordination-driven assembly of Cu 2+ with protoporphyrin IX (Pp IX) for electrochemically sensing histidine (His) in blood. More interestingly, the Cu NFs-modified electrode could exhibit characteristic electrochemical peaks of CuCl at a lower and more favorable potential, which is practically advantageous. Especially, the CuCl signals would undergo a selective enhancement in the presence of His because of the specific coordination between copper ions and the imidazole moiety in His. This mechanism forms the basis of a proposed electrochemical strategy for highly selective His detection, exhibiting a linear range of 0.05 μM–100 μM. The limit of detection (LOD) for this method was calculated to be about 0.014 μM. Furthermore, the newly developed electrochemical strategy was applied to detect His in blood samples, showing analytical performances comparatively superior to those of the documented methods. This low-potential electrochemical detection strategy with enhanced signal output shows significant potential for use in the disease diagnostics and food safety analysis fields.
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