AQUATIC TOXICOLOGY
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2024
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IF 4.1
Shuquan Ding,Shunli Che,Mengting Huang,Yuan Ma,Longteng Shen,Jianbin Feng,Xilei Li
Nanopolystyrene (NP) pollution in aquatic environments has become an increasing concern. Phoxim (PHO), one of the major organophosphorus pesticides, has also been detected in aquatic environments, posing serious health risks to crustaceans. This study aimed to assess the detrimental effects of NP and/or PHO exposure at environmentally relevant concentrations on the intestinal histopathology, intestinal microbiota, and metabolome of adult crabs ( Eriocheir sinensis ) for 21 days. Our study revealed significant histopathological abnormalities in the intestines. In all the exposure groups, there was a discovery of vacuolar degeneration occurring in epithelial cells. Additionally, the peritrophic membrane exhibited thinning after NP or PHO single exposure, while thickening was observed after co-exposure. Exposure to NP and/or PHO disrupted the intestinal microbiota homeostasis, as evidenced by the proliferation of pathogenic bacteria and suppression of beneficial bacteria. Notably, PHO exposure resulted in increased abundance of pathogenic bacteria ( Spiroplasma and Arcobacter ) and decreased abundance of beneficial bacteria ( Bacteroides ). Analysis of the metabolome revealed that exposure to NP and/or PHO led to alterations in the metabolic profile as well as several critical pathways. Among these, the upregulation of arachidonic acid metabolism, ABC transporters, and biosynthesis of amino acids was observed in both NP single exposure and co-exposure, while PHO single exposure downregulated these pathways. Additionally, NP and/or PHO exposure downregulated neuroactive ligand-receptor interaction. Spearman correlation analysis revealed that the significant reduction of some differentially expressed metabolites (DEMs) was potentially regulated by the low-abundance bacterial genera following exposure to NP and/or PHO. And these DEMs have a role in anti-inflammatory or antioxidant properties. Collectively, our results offer novel perspectives on the intestinal toxicity of crustaceans by NP and/or PHO at environmentally relevant concentrations.
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Science China-Chemistry
·
2025
·
IF 9.7
Bai Rui,Li Junpeng,Chen Wei,He Yi,Zhou Xudong,Tian Xiaochun,Ren Chongyuan,Chen Liqi,Xiao Yong,Zhao Feng
The integration of microorganisms and photosensitizers presents a promising approach to chemical production utilizing solar energy. However, the current system construction process remains complex. Herein, we introduce a straightforward and efficient solar-to-chemical conversion system that combines the dissolved photosensitizer Eosin Y with the non-photosynthetic bacterium Moorella thermoacetica . Under light radiation, acetate production increased to 5.1 µM h −1 µM −1 catalyst, exceeding the previously reported maximum by 5.9-fold, with a quantum efficiency of 17.6%. The soluble photosensitizer EY can penetrate the cell and directly engage in intracellular energy metabolism, significantly enhancing intracellular ATP and NADPH/NADP + levels. Within this biohybrid system, sacrificial agent triethanolamine played a dual role: (1) providing continuous photoelectron generation by Eosin Y, enhancing intracellular reducing power, and facilitating carbon fixation via the Wood-Ljungdahl pathway; and (2) its oxidation product, formaldehyde, served as a critical intermediate and a direct precursor for methylenete-trahydrofolate in the Wood-Ljungdahl pathway, consequently simplifying reaction steps and markedly boosting acetate yield. This study provides a simple microorganism-photosensitizer biohybrid system to produce acetate and light on the multifaceted roles of sacrificial agents, paving the development of efficient solar energy conversion with nonphotosynthetic bacteria.
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Asian Journal of Pharmaceutical Sciences
·
2025
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IF 11.9
Zhiyuan Tang,Yuening Sun,Quanhua Yi,Qian Ding,Yang Ding,Jianfei Huang
The effective intracellular accumulation of doxorubicin (DOX) is crucial for improving antitumor efficacy, which is severely impeded by limited drug penetration, uncontrollable drug release, and drug resistance. In this study, a thermal-deformative polymer embedding ultrasmall ceria (CeO 2 ) was rationally designed for deep tumor drug shuttling and hypoxia reversal to improve chemotherapy. Structurally, the CeO 2 nanozyme was covalently grafted with a polymer of p(NIPAM-co-AM) that could sharply shrink for DOX loading, which was consolidated with polydopamine (PDA) film encapsulation. Thereafter, a tumor penetration guide of apolipoprotein A-I (apoA-I) conjugated iRGD peptide (apoA-I-iRGD) was further decorated onto the PDA shell via Michael addition for preparing CeO 2 P/DOX@iAPDA. With the aid of apoA-I-iRGD, CeO 2 P/DOX@iAPDA penetrated both the tumor spheroids (∼78 μm) and the tumors of the mouse model deeply. After internalization by tumor cells and triggering by low pH in lysosomes, rapid DOX release was achieved by peeling off the PDA shell and thermosensitive deformation of p(NIPAM-co-AM). CeO 2 P/DOX@iAPDA provided 66.4% tumor suppression in 4T1-derived tumor spheroids and 63.2% in 4T1-tumor-bearing mice, respectively. Preliminary mechanistic research involving western blotting and immunohistochemistry revealed that CeO 2 P/DOX@iAPDA reversed resistance through the through HIF-1α-P-gp/lipid axis. Collectively, this study intelligently integrated CeO 2 nanozymes, temperature-sensitive polymers, and imitated biochemical modifications to improve chemotherapy for breast cancer.
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Acta Biomaterialia
·
2018
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IF 6.383
Yaqin Liu,Tao Li,Hongshi Ma,Dong Zhai,Cuijun Deng,Jinwu Wang,Shangjun Zhuo,Jiang Chang,Chengtie Wu
For treatment of bone tumor and regeneration of bone defects, the biomaterials should possess the ability to kill tumor cells and regenerate bone defect simultaneously. To date, there are a few biomaterials possessing such dual functions, the disadvantages, however, such as long-term toxicity and degradation, restrict their application. Although bioactive elements have been incorporated into biomaterials to improve their osteogenic activity, there is no report about elements-induced functional scaffolds for photothermal tumor therapy. Herein, the elements (Cu, Fe, Mn, Co)-doped bioactive glass-ceramic (BGC) scaffolds with photothermal effect and osteogenic differentiation ability were prepared via 3D-printing method. Moreover, the photothermal anti-tumor effect and osteogenic activity of these scaffolds were systematically investigated. The prepared elements-doped scaffolds possessed excellent photothermal performance, which displayed a trend, 5Cu-BGC > 5Fe-BGC > 5Mn-BGC > 5Co-BGC, in this study. The final temperature of elements-doped scaffolds can be well controlled by altering the doping element categories, contents and laser power density. Additionally, the hyperthermia induced by 5Cu-BGC, 5Fe-BGC and 5Mn-BGC effectively killed tumor cells in vitro and inhibited tumor growth in vivo . More importantly, 5Fe-BGC and 5Mn-BGC scaffolds could promote rabbit bone mesenchymal stem cells (rBMSCs) adhesion, and the ionic products released from elements-doped scaffolds significantly stimulated the osteogenic differentiation of bone-forming cells. These results suggested that 5Fe-BGC and 5Mn-BGC scaffolds possessed promising potential for photothermal treatment of bone tumor and at the same time for stimulating bone regeneration , representing a smart strategy for the treatment of bone tumors by combining dual functional bioactive ions with tissue engineering scaffolds. Statement of Significance The major innovation of this study is that we fabricated the elements (Cu, Fe, Mn, Co)-doped bioactive scaffolds via 3D printing technique and found that they possess distinct photothermal performance and osteogenic differentiation ability. To the best of our knowledge, there is no report about elements-doped scaffolds for photothermal therapy of bone tumor. This is an important research advance by combining the photothermal effect and osteogenic differentiation activity of bioactive elements in the scaffold system for potential bone tumor therapy and bone reconstruction . We optimized the elements-doped scaffolds and found the photothermal effect of elements-doped scaffolds (5Cu-BGC, 5Fe-BGC, 5Mn-BGC) could effectively kill tumor cells in vivo . The photothermal performance of elements-doped scaffolds follows a trend: 5Cu-BGC > 5Fe-BGC > 5Mn-BGC > 5Co-BGC > BGC. Compared to traditional nano-sized photothermal agents, bioactive elements-induced functional scaffolds have better biosecurity and bioactivity. Furthermore, 5Fe-BGC and 5Mn-BGC scaffolds displayed excellent bone-forming activity by stimulating the osteogenic differentiation of bone-forming cells. The major significance of the study is that the elements-doped bioactive glass-ceramics (5Fe-BGC, 5Mn-BGC) have great potential to be used as bifunctional scaffolds for photothermal tumor therapy and bone regeneration, representing a smart strategy for the treatment of bone tumors by combining dual functional bioactive ions with tissue engineering scaffolds.
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ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Chenxu Zhu,Chuang Li,Yibo Du,Shi Chen,Xingliang Liu,Lixin Liu,Yongming Chen
Neutrophil extracellular traps (NETs) induce tumor metastasis by riding tumor cells through interaction via the transmembrane protein CCDC25 on the surface of cancer cells. Recently, we applied positively charged polyamino acids to neutralize negatively charged NET-DNA and inhibit tumor metastasis as approved on several metastasis models. To elucidate the exact polymer structures on performance, herein, we precisely synthesized a series of cationic oligopeptides with defined numbers of arginine (R) and glycine (G). The oligopeptides R5, R7, R9, and R5G4 showed much better binding affinity and lower cytotoxicity than R3 and R3G6. From the pull-down assay, the oligoarginines, R5, R7, and R9, inhibited CCDC25 interaction with NET-DNA because of stronger competitive interaction than myeloperoxidase (MPO) and citrullinated histone H3 (H3Cit) in NETs. The subsequent inhibition of cell migration and tumor metastasis in triple-negative breast cancer (TNBC) mouse models demonstrated that R5, R7, and R9 efficiently prevented tumor cell metastasis to the liver and lung. This was corroborated by the distribution of oligoarginines in the liver and lung, which reduced NETs accumulation. Thus, in this study, we screened out oligoarginines with a precise number of units to inhibit TNBC metastasis to distant organs, which may benefit in the decreasing mortality rate because of tumor metastasis. With their low cytotoxicity, oligoarginines represent a major advancement for the clinical treatment of metastatic diseases.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
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IF 8.1
Qian Liu,Changdi Wang,Hao Wang,Lu Li,Xingrong Zhang,Ruiyang Zhao,Jishu Han,Lei Wang
Hydrogen energy produced through photocatalytic water splitting is widely recognized as a viable solution to the depletion of non-renewable energy resources and ecological contamination concerns. This work designed and synthesized the MOF-derived hollow octahedral Co x P/MOF-801 p-n heterojunction photocatalyst . According to the result of hydrogen production test, the hydrogen production amount of Co x P/MOF-801-2 could reach 25.31 mmol g −1 under light irradiation for 5 h. The hollow octahedral structure and p-n heterojunction of the photocatalyst helped to enhance the light trapping ability and promote carrier transfer, resulting in improved performance of photocatalytic hydrogen production. To sum up, the design of hollow octahedral Co x P/MOF-801 photocatalyst effectively improved the photocatalytic hydrogen production performance.
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Frontiers in Cellular and Infection Microbiology
·
2025
·
IF 4.8
Feng-Hua Xu,Xiao Sun,Jun Zhu,Ling-Yang Kong,Yuan Chang,Ning Li,Wen-Xiang Hui,Cong-Peng Zhang,Yi-Ming Cheng,Wen-Xin Han,Zhi-Min Tian,Yan-Ning Qiao,Dong-feng Chen,Lei Liu,Da-Yun Feng,Jing Han
Background Recent research has made significant progress in elucidating gastrointestinal complications following acute cerebral infarction (ACI), which includes disorders in intestinal motility and dysbiosis of the gut microbiota. Nevertheless, the role of the gut (which is acknowledged as being the largest immune organ) in the immunoreactive effects of polydopamine nanoparticles (PDA) on acute ischemic stroke remains inadequately understood. In addition to its function in nutrient absorption, the gut acts as a protective barrier against microbes. Systemic immune responses, which are triggered by the disruption of gut barrier integrity, are considered as one of the mechanisms underlying acute ischemic stroke, with the gut-brain axis (GBA) playing a pivotal role in this process. Methods In this study, we used a PDA intervention in an ACI model to investigate ACI-like behavior, intestinal barrier function, central and peripheral inflammation, and hippocampal neuron excitability, thus aiming to elucidate the mechanisms through which PDA improves ACI via the GBA. Results Our findings indicated that as ACI mice experienced dysbiosis of the gut microbiota and intestinal barrier damage, the levels of proinflammatory factors in the serum and brain significantly increased. Additionally, the activation of astrocytes in the hippocampal region and neuronal apoptosis were observed in ACI mice. Importantly, our study is the first to provide evidence demonstrating that PDA effectively suppresses the neuroimmune interactions of the gut-brain axis and significantly improves intestinal epithelial barrier integrity. Conclusion We hope that our discoveries will serve as a foundation for further explorations of the therapeutic mechanisms of PDA in ACI, particularly in elucidating the protective roles of gut microbiota and intestinal barrier function, as well as in the development of more targeted clinical interventions for ACI.
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Small
·
2024
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IF 13
Zining Hao,Shu Guo,Wenkang Tu,Qiang Wang,Jing Wang,Xuwu Zhang,Yuchu He,Dawei Gao
Cellular senescence, a vulnerable state of growth arrest, has been regarded as a potential strategy to weaken the resistance of tumor cells, leading to dramatic improvements in treatment efficacy. However, a selective and efficient strategy for inducing local tumor cellular senescence has not yet been reported. Herein, piezoelectric catalysis is utilized to reduce intracellular NAD + to NADH for local tumor cell senescence for the first time. In detail, a biocompatible nanomedicine (BTO/Rh-D@M) is constructed by wrapping the piezoelectric BaTiO 3 /(Cp * RhCl 2 ) 2 (BTO/Rh) and doxorubicin (DOX) in the homologous cytomembrane with tumor target. After tumors are stimulated by ultrasound, negative and positive charges are generated on the BTO/Rh by piezoelectric catalysis, which reduce the intracellular NAD + to NADH for cellular senescence and oxidize H 2 O to reactive oxygen species (ROS) for mitochondrial damage. Thus, the therapeutic efficacy of tumor immunogenic cell death-induced chemo-immunotherapy is boosted by combining cellular senescence, DOX, and ROS. The results indicate that 23.9% of the piezoelectric catalysis-treated tumor cells senesced, and solid tumors in mice disappeared completely after therapy. Collectively, this study highlights a novel strategy to realize cellular senescence utilizing piezoelectric catalysis and the significance of inducing tumor cellular senescence to improve therapeutic efficacy.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
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IF 6.2
Shunli Che,Mengting Huang,Huiying Ma,Zhicheng Wan,Jianbin Feng,Shuquan Ding,Xilei Li
Nanopolystyrene (NP) and cadmium (Cd) are ubiquitous contaminants in aquatic systems. The present study aimed to investigate the toxic effects of exposure to ambient concentrations of NP and/or Cd on the intestinal tract of the Chinese mitten crab ( Eriocheir sinensis ). Exposure to NP and/or Cd induced oxidative stress, as evidenced by a significant increase in lipid peroxide content (LPO), total antioxidant capacity (T-AOC), and peroxidase activity (POD), and significant decreases in superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities in E. sinensis . In addition, exposure to NP and/or Cd imbalanced the homeostasis of the intestinal microbiota, as demonstrated by the significantly increased abundance of Spiroplasma . Transcriptomic and metabolomic analyses were performed to investigate the mechanisms underlying intestinal toxicity. Our results showed that ferroptosis, ABC transporters, phosphotransferase system, apoptosis, and leukocyte transendothelial migration were disturbed after exposure to NP and/or Cd. In particular, Cd exposure affected mucin type O-glycan biosynthesis, purine metabolism, and neuroactive ligand-receptor interaction. Intriguingly, co-exposure to NP and Cd might mitigate intestinal toxicity by decreasing oxidative stress and affecting these pathways. Taken together, our study clearly demonstrates that exposure to NP and/or Cd at environmentally relevant concentrations causes intestinal toxicity in E. sinensis .
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WATER RESEARCH
·
2025
·
IF 12.4
Lingyou Meng,Zhuo Li,Binhong Qu,Peng Li,Jianhui Sun,Yang Qu,Ziqing Zhang,Liqiang Jing
The ultrasensitive, visual and intelligent identification of persistent chlorophenols (CPs) in complex drinking and environmental water matrices is highly desirable. In this study, we have established an interesting colorimetric dye eosin Y (EY)-molecular imprinted Bi 4 O 5 Br 2 nanosheet photosensitization sensing system for CPs detection, in which the key is the rapid EY photosensitization decolorization to be efficiently inhibited in the presence of CPs. It displays an ultrasensitive detection of CPs, especially for the typical 2,4,6-trichlorophenol (TCP) across from 10 ng·L −1 to 1 mg·L −1 , achieving a limit of detection of 7 ng·L −1 with remarkable selectivity. By means of in-situ Fourier transform infrared spectroscopy, time-resolved laser flash photolysis spectroscopy, and theoretical calculations, the outstanding performance is attributed to (i) the efficient transfer of triplet excited state electrons from EY to the ultrathin Bi 4 O 5 Br 2 (∼4 nm) nanosheets through energy band alignment and (ii) enhanced TCP selective adsorption resulting from a planar adsorption configuration induced by multiple Bi–Cl interactions, along with specific molecular imprinting recognition sites. Additionally, a real-time intelligent sensing platform was further engineered by integrating automatic sampling, an optical fiber source and a smartphone with color recognition software, enabling convenient and visual detection of TCP in challenging water environments. The sensing system exhibited excellent performance in detecting TCP across various actual water matrices, achieving acceptable recovery rates ranging from 93.33% to 113.33% with the relative standard deviations between 0.80% and 11.31%. It also demonstrated significant advantages over the ultra-performance liquid chromatography-tandem mass spectrometry, particularly regarding speed, simplicity and sensitivity, thus indicating substantial potential for practical applications in water quality monitoring.
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ChemistrySelect
·
2025
·Vol. 10
·期 28
·e00259
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IF 2
Bo Yang,Hai Xing,Yang Xia,Xin Wang,Ningning Liu
Graphical ZIF-8 was synthesized through an in-situ growth method within a PVDF membrane matrix. The PVDF/ZIF-8 composite membrane was subsequently prepared via the phase inversion method using immersion precipitation. The ZIF-8 particles were uniformly distributed in the PVDF/ZIF-8 composite membrane. The widespread use of organic dyes in industrial processes has led to increasing environmental pollution due to the discharge of dye-contaminated wastewater, necessitating more efficient dye removal methods. In this study, a poly(vinylidene fluoride) (PVDF)/ZIF-8 composite membrane was fabricated via a phase inversion method using immersion precipitation, with ZIF-8 synthesized via in-situ growth within the membrane matrix. The composite membranes were thoroughly characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and thermogravimetric analysis (TGA). The ZIF-8 particles were uniformly distributed in the PVDF/ZIF-8 composite membrane. The adsorption performance of the PVDF/ZIF-8 composite membrane was evaluated for methyl green (MG) removal. At 313 K with an adsorbent dose of 10 mg, the membrane exhibited an adsorption capacity of 172.30 mg g⁻¹ and a removal ratio of 87.21%, demonstrating that the incorporation of ZIF-8 significantly enhanced adsorption performance. Adsorption isotherm studies revealed that the process followed the Freundlich model, while kinetic analysis indicated a pseudo-second-order mechanism. Additionally, thermodynamic investigations suggested that the adsorption was endothermic and spontaneous. The major driving force for adsorption was identified as electrostatic interactions between the dye and the membrane. These findings highlight the potential of PVDF/ZIF-8 composite membranes for effective dye removal from wastewater.
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ACS Sustainable Chemistry & Engineering
·
2025
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IF 7.3
Chang-Wei Zhang,Jian-Zhong Ye,Xing-Ying Xue,Wen-Jun Li,Hong-Xia Chen,Cheng-Zhang Wang
Baphicacanthus cusia (B. cusia) was traditionally used as raw material to prepare indigo naturalis; however, many factories have been shut down by local governments on account of their serious pollution contributions. In this study, a green and sustainable production of B. cusia leaf extracts is proposed. Namely, new indigo extract (NIE) and phenolic acid extract (PAE) were produced in an integrated fashion by enzyme transformation coupled membrane separation and freeze-drying technologies. The small-scale process was optimized, and its feasibility was verified by a pilot experiment. Moreover, the physicochemical properties, dyeing effect, and wound-healing ability of the two extracts were systematically evaluated. The NIE could be obtained within 1 day and exhibited more than 20 times the indigo content and 1.8 times the pure indigo yield than indigo naturalis. Moreover, the NIE showed superior dye-uptake rate and washing and rubbing fastness on pure cotton poplin compared with natural indigo from India. The PAE exhibited excellent broad spectrum antibacterial activities, especially S. aureus (IC50 = 32 μg/mL). Significantly, the PAE had no toxicity and presented over 98% of the wound-healing rate on mice by increasing their collagen fiber content. All of above work is of great significance for the extension and sustained healthy development of a B. cusia industry chain.
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