JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Xiao Wang,Ya Zhao,Xin Qi,Tiantian Zhao,Xiupin Wang,Fei Ma,Liangxiao Zhang,Qi Zhang,Peiwu Li
Aflatoxins seriously threaten human health and food safety, and early warning benefits the reasonable use of control measures to reduce aflatoxin contamination. In this study, a novel method for quantifying aflatoxins and their precursors in the aflatoxin biosynthesis pathway was developed by combining ultra-high performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) with quantitative analysis of multi-components by a single marker (QAMS). The stability of the relative correction factor (RCF) of QAMS was then systematically evaluated. The validation results showed that the relative deviation (RD) between QAMS and the external standard method (ESM) was less than 11.7%, indicating that the established QAMS method could replace ESM without the use of reference standards. This method was successfully employed to compare the time-course changes of metabolites in the aflatoxin biosynthesis pathway of Aspergillus flavus and Aspergillus parasitica. As a result, the precursors of (1'S,5′R)−5′-Hydroxyaverantin (HAVN) and Versicolorin B (VerB) could be used as potential markers for the early warning of aflatoxin contamination. This study provided a quantitative method of aflatoxins and their precursors in the biosynthesis pathway, and may serve as a reference for the extension of quantitative studies on other metabolic pathways.
研究领域:
食品安全
·
分子生物学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Yang Peizhou,Xiao Wei,Lu Shuhua,Jiang Shuying,Jiang Suwei,Chen Jianchao,Wu Wenjing,Zheng Zhi,Jiang Shaotong
Aflatoxin B1 (AFB1) contaminates rice during harvest or storage and causes a considerable risk to human and animal health. In this study, Trametes versicolor AFB1–degrading enzyme (TV–AFB1D) gene recombinantly expressed in engineered E. coli BL21 (DE3) and Saccharomyces cerevisiae. The TV–AFB1D enzymatic characteristics and AFB1 degradation efficiency in contaminated rice were investigated. Results showed that the size of recombinant TV-AFB1D expressing in E. coli BL21 (DE3) and S. cerevisiae was appropriately 77 KDa. The kinetic equation of TV-AFB1D was y = 0.01671x + 1.80756 (R2 = 0.994, Km = 9.24 mM, and Vmax = 553.23 mM/min). The Kcat and Kcat/Km values of TV-AFB1D were 0.07392 (s−1) and 8 M−1 s−1, respectively. The AFB1 concentration of contaminated rice decreased from 100 μg/ml to 32.6 μg/ml after treatment at 32°C for 5 h under the catabolism of TV-AFB1D. S. cerevisiae engineered strains carrying aldehyde oxidase 1 (AOX1) and Cauliflower mosaic virus 35 S (CaMV 35 S) promoters caused the residual AFB1 contents, respectively, decreased to 3.4 and 2.9 μg/g from the initial AFB1 content of 7.4 μg/g after 24 h of fermentation using AFB1-contaminated rice as substrate. The AFB1 degradation rates of S. cerevisiae engineered strains carrying AOX1 and CaMV promoters were 54 and 61%, respectively. Engineered S. cerevisiae strains integrated with TV-AFB1D expression cassettes were developed to simultaneously degrade AFB1 and produce ethanol using AFB1-contaminated rice as substrate. Thus, TV-AFB1D has significant application potential in the AFB1 decomposition from contaminated agricultural products.
研究领域:
食品科学
·
微生物学
·
生物化学
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DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2024
·Vol. 235
·期 4
·1-12
·
IF 3.8
Liu Yonghong,Yang Xiaoxiao,Wang Ning,Wang Quanhong
While high-efficiency photocatalysts have been extensively researched, there is still a scarcity of reports focusing on enhancing the practical application conditions of low-cost engineering photocatalysts. In this study, a 13.8-L continuous streamer catalytic reactor was developed utilizing low-cost self-made TiO 2 /ACF photocatalyst. Employing the Box-Behnke response surface method, the study assessed the reactor’s efficiency in treating organic wastewater using methylene blue (MB) as the model pollutant. The TiO 2 /ACF photocatalysis was characterized by XRD, EDS, and SEM, and the results showed that the nano-TiO 2 was uniformly loaded with the surface of activated carbon fibers in a lamellar form. Results indicated that with a single cycle time of 134 min and a catalyst amount of 95 cm × 95 cm, the average decolorization rate for 3 mg/L MB wastewater was 90.63%, following quasi-second-order kinetics. The MB wastewater’s decolorization efficiency swiftly reached 89.8% within the initial 120 min, maintaining around 90% during continuous 600-min treatment. The optimal catalyst condition was identified, enhancing photodegradation efficiency of MB organic molecules. The developed photocatalytic equipment exhibited high efficiency and stability in degrading low-concentration organic molecules.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
Antibiotics-Basel
·
2025
·Vol. 14
·期 5
·499
·
IF 4.6
Liyang Wang,Junying He,Hanzhong Feng,Qian Li,Meirong Song,Haoran Gou,Yongxing He,Kui Zhu
Background:Fungal infections pose an increasingly predominant threat to human and animal health. Modified compounds derived from chemo-diverse natural products offer enhanced therapeutic efficacies and promising approaches to combat life-threatening fungal pathogens.Methods:We performed biosynthetic gene clusters analysis of 2,4-diacetylchloroglucoside (DAPG) in 4292 shotgun metagenomes samples from the healthy and diseased skin. Then, we assessed the antifungal activity of DAPG and the derivative 2,4-diproylphloroglucinol (DPPG) against pathogenic fungi by minimum inhibitory concentrations. The inhibitory effects of DPPG were measured using hyphal growth assay and spore germination assay. Concurrently, the mechanism of DPPG onAspergillus fumigatuswas investigated in membrane permeability and fluidity. The therapeutic efficacy was evaluated in aGalleria mellonellainfection model.Results:We observed a significantly higher abundance of bacteria harboring DAPG biosynthetic clusters on healthy skin compared to diseased skin. Further, we designed and synthesized a series of phloroglucinol derivatives based on DAPG and obtained an antifungal candidate DPPG. DPPG not only exhibited robust antifungal activity againstAspergillusspp. andCandidaspp. but also impaired hyphal growth and spore germination ofA. fumigatusin vitro. A mechanism study showed that DPPG reduced membrane fluidity and increased the leakage of cellular contents, resulting in membrane perturbation and fungal death. Lastly, the therapeutic efficacy of DPPG was confirmed in aG. mellonellainfection model.Conclusions:Our study demonstrates that DPPG is a potent scaffold to combat invasive fungal infections.
研究领域:
微生物学
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药理学
·
感染病学
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Jing Xu,Kangle Wan,Jianjie Wu,Yunchao Wu,Wei Yan,Lichuan Zhang,Yuanping Chen,Yingcong Wei
During the piezoelectric photocatalytic process, the surface curvature of the piezoelectric photocatalyst undergoes dynamic changes under the influence of external mechanical forces. However, the correlation between the surface curvature and catalytic performance remains largely unexplored. Here, we demonstrate that decreasing the radius of curvature of MoSe2 significantly enhances its piezoelectric-photocatalytic activity for C–N coupling reactions, enabling imine synthesis from benzylamine. Comprehensive characterization and DFT calculations together show that the piezoelectric effect in the curved MoSe2 structure inhibits photogenerated charge carriers from recombination, improving carrier utilization efficiency. Additionally, the surface curvature effect induced by ultrasonic driving reduces the band gap. It promotes the effective transfer of electrons from Mo atoms in MoSe2 to −NH2 in benzylamine, thereby facilitating the activation of the benzylamine molecule. As a result, the synergistic effects of the piezoelectric and curvature characteristics significantly enhance the photocatalytic performance of MoSe2 toward the C–N coupling of benzylamine. This study not only provides new insights into interfacial reaction mechanisms in piezoelectric photocatalysis but also offers a novel perspective for designing high-performance catalytic systems.
研究领域:
材料科学
·
催化化学
·
理论计算
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Lei Yao,Chao Hong,Yangxu Qi,Lie Wu
In this study, we developed a novel magnetic composite of walnut shell biochar (WSB) and modified it with ZIF-7 for the purpose of removing Co 2+ ions. We conducted a comprehensive evaluation of its performance in Co 2+ removal, considering various factors such as contact time, temperature, adsorbent dose, pH, and initial Co 2+ concentration. Utilizing response surface methodology-central composite design (RSM-CCD), we examined the interactions between these variables. The specific surface area and magnetic saturation value of the WSB/MnFe 2 O 4 /ZIF-7 composite were determined as 170.560 m 2 .g −1 and 23.469 emu.g −1 , respectively. ANOVA analysis indicated that our model effectively captures the interactions between variables, with a high R 2 value of 0.9982, explaining 99.82 % of the experimental data. Optimal conditions for maximum Co 2+ adsorption efficiency (99.05 %) were identified as follows: a contact time of 44.82 min, pH of 6.04, adsorbent dose of 0.947 mg.L -1 , temperature of 25.55 °C, and Co 2+ concentration of 11.13 mg.g −1 . Kinetic studies revealed that the Pseudo 2nd order kinetic model provided the best fit to the experimental data. The negative entropy parameter indicated a decrease in disorderliness during the adsorption process, while the exothermic nature of adsorption was confirmed by enthalpy values of −92.751 and −40.511 kJ.mol −1 for WSB/MnFe 2 O 4 /ZIF-7 and WSB, respectively. Furthermore, the Langmuir isotherm model demonstrated excellent fitting to the isotherm results, suggesting the dominance of homogeneous surfaces in adsorption. The maximum adsorption capacities for Co 2+ using WSB/MnFe 2 O 4 /ZIF-7 and WSB were determined to be 29.26 and 22.39 mg.g −1 , respectively. The WSB/MnFe 2 O 4 /ZIF-7 magnetic composite demonstrates promising potential for efficiently removing Co 2+ from both natural water sources and industrial wastewater, highlighting its versatility and effectiveness in environmental remediation applications.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 3
·1-18
·
IF 2.4
Kong Lingnan,Abulizi Abudula,Cong Jingjing,Meng Weihan,Chang Yaqing,Sun Zhihui
The cytochrome P4503A ( CYP3A ) subfamily has been well characterized in vertebrates, where it plays a pivotal role in xenobiotic detoxification and the regulation of steroid hormone levels. However, their molecular features and functions in invertebrates remain poorly understood. The sea cucumber ( Apostichopus japonicus ) is not only a commercially significant mariculture species but also an excellent model for studying organ development and evolutionary biology. In this study, we identified seven CYP3A genes in A. japonicus using gonadal transcriptomic data. These genes exhibited ubiquitous expression across adult tissues, with significantly higher levels in ovarian tissues compared to testes tissues. Protein sequence analysis revealed that the catalytic residues Ala 306 -Thr 310 -Cys 437 , which are critical for aromatase activity, are highly conserved. This suggests that CYP3A proteins may mediate aromatization-like reactions similar to CYP19A in vertebrates. Additionally, dietary administration of the aromatase inhibitor letrozole led to histological changes in the testes and a reduction in the diameter of large oocytes in the ovaries. These findings provide valuable insights into the molecular mechanisms underlying gonadal development in sea cucumbers and lay the groundwork for future research on the artificial induction of sex reversal in echinoderms.
研究领域:
分子生物学
·
海洋生物学
·
进化生物学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Yingli Guo,Xingping Zhang,Hualin Yang,Yu Zhou
Background Ochratoxin A (OTA) is a widespread mycotoxin with nephrotoxic, neurotoxic and immunotoxic properties. It exhibits elevated thermal stability and a prolonged half-life and is resistant to removal. Long-term exposure to OTA increases the risk of carcinogenesis and influences human health. The detection methods of single signal mode had poor anti-interference ability and were prone to false positive results, while the detection method of multi-signal mode has higher adaptability to conditions and can offset environmental interference through self-correction. Therefore, proposing multi-signal and high-sensitivity strategies to detect OTA levels in various foods is all-important. Results A triple-readout immunoassay based on copper ions trigger was developed for detecting Ochratoxin A. The ascorbic acid 2-phosphate (AAP) was hydrolyzed to ascorbic acid (AA) by alkaline phosphatase (ALP). The unreacted AAP rapidly coordinated with Cu 2+ to produce green Cu 2+ -AAP complexes which emitted blue/green (B/G) signals. Meanwhile, Cu 2+ oxidized AA to produce dehydroascorbic acid (DHAA) which reacted with o-phenylenediamine (OPD) to form the blue-fluorescence quinoxaline derivative (DFQ). Simultaneously, Cu 2+ oxidized the unreacted OPD to form yellow 2,3-diaminophenazine (DAP) which produced yellow signals (Y). The B/G and Y was read by a smartphone. The limits of detection (LOD) of B/G, fluorescence and Y value readout models were 0.13 ng/mL, 0.16 ng/mL and 0.15 ng/mL. Their linear ranges were 6.25–100 ng/mL, 6.25–200 ng/mL and 3.13–12.5 ng/mL. Besides, all three readout models showed excellent specificity for OTA, and the recovery rates were satisfactory from 94.79 % to 112.68 %. Significance A triple-readout immunoassay based on copper ions trigger for the detection of ochratoxin A was constructed. The materials for detection are accessible and economical. Triple signal modes not only complement and verify each other to improve the reliability of the results but also can be selected according to the detection scenes to increase the freedom of detection. It can be used to detect other targets by altering the coated antigen and its corresponding antibody.
研究领域:
食品安全检测
·
分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jiaying Fang,Dantong Li,Jie Li,Qianqian Wang,Juntao Wang,Yixuanfei Gao,Yihang He,Chenhuan Wang
Aflatoxins are the most toxic and high-profile mycotoxins with high occurrence in grain and nuts. To effectively adsorb and sensitively detect aflatoxins in peanuts, two novel covalent organic frameworks (TF-COF, TH-COF) were fabricated by Schiff-base reaction of N,N,N′,N′-tetra ( p -aminophenyl)- p -phenylenediamine with 2,3,5,6-tetra-fluoroterephthaldehyde and terephthalendehyde, respectively. The TF-COF shows higher adsorption capacity (35–44 mg g −1 ) for aflatoxins than TH-COF (26–37 mg g −1 ) because abundant fluorine functional groups in TF-COF offer more adsorption sites. A sensitive and practical method for determining aflatoxins (G 2 , G 1 , B 2 and B 1 ) in peanuts was developed by combining TF-COF-based extraction technology with high performance liquid chromatography-fluorescence detector. The method provides outstanding performance with linear range of 0.025 ng g −1 -20.0 ng g −1 , detection limits of 0.008 ng g −1 -0.15 ng g −1 , recovery of 86.3 %–116 %, and relative standard deviation below 7.8 %. This study opens a new avenue to develop functionalized COF with distinguished performance for adsorbing hazards in food and/or environmental samples.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2019
·
IF 2.606
Liqing Chen,Atef Mohammed Qasem Ahmed,Yibin Deng,Dingyun Cao,Huanhuan Du,Jinghao Cui,Beom-Jin Lee,Qingri Cao
Low encapsulation efficiency (EE) and high initial burst release are key issues for the development of microspheres loading highly water-soluble peptide drugs. In this study, novel triptorelin acetate-loaded microspheres with high EE and low initial burst release were prepared by a liquid/oil/oil (L/O/O) phase separation method using poly(lactic- co -glycolic acid) (PLGA) as the sustained-release carrier. The formulation of the microspheres and the preparation process were optimized in terms of particle size, surface morphology, EE, and in vitro drug release. Importantly, PLGA concentration, solvent and non-solvent ratio, solidification solvent volume, and solidification speed were found to significantly affect the properties of the obtained microspheres. The optimal microspheres present 50–100 μm spherical-shaped particles with remarkably high drug loading and EE. The in vitro drug release of these microspheres exhibit a delayed-release manner, starting to release drug at the third week and displaying approximately 20% drug release at day 28. Fourier transform infrared spectroscopy and differential scanning calorimetry results show that triptorelin acetate slightly interacted with PLGA, indicating that the drug was successfully encapsulated into PLGA microspheres. The drug content in the microspheres had no significant change after the 12-month storage at 4 °C, suggesting their excellent long-term stability. In conclusion, novel triptorelin acetate-loaded PLGA microspheres have been achieved via the L/O/O phase separation method to effectively improve the EE and reduce the initial burst release.
研究领域:
药剂学
·
生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF MICROENCAPSULATION
·
2019
·
IF 2.04
Zhaoying Wu,Mengqing Zhao,Wei Zhang,Zhao Yang,Shuxin Xu,Qing Shang
The purpose of this study was to investigate the influences of drying methods on the risperidone (RIS) release profiles of RIS-loaded PLGA microspheres. These microspheres were fabricated with an O/W emulsion solvent evaporation method. The wet microspheres were dried with freeze drying and vacuum drying methods. The microspheres were mono-dispersed spheres with an average diameter of 100 μm. Studies found that drying methods had great influence on the porosity, morphology, and release profiles of RIS-loaded PLGA microspheres. Specifically, the freeze-dried microspheres had higher porosity (78.46 ± 1.64%) than those vacuum-dried ones (52.45 ± 2.68%), and they showed higher RIS release rates (p < 0.05). In the accelerated release tests (45 °C), these microspheres dried under the pressures of 700 mmHg and 200 mmHg gave faster release rates than those ones dried under the pressure of 450 mmHg. Importantly, the accelerated release test (45 °C) had a high correlation with the real-time test (37 °C) (R2 > 0.99). These studies exhibited a significance in the precise preparation of RIS-loaded PLGA microspheres.
研究领域:
药学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2018
·Vol. 6
·期 24
·6416-6422
·
IF 5.976
Taoping Wang,Mingtong Li,Hui Zhang,Yunyu Sun,Bin Dong
Smart actuators have recently been widely studied due to their potential applications in the fields of wearable devices, artificial muscles and microrobots. However, it is still challenging to develop smart actuators with a fast response time, large deformation, multi-stimuli response ability and programmable shape-changing capability. Here, we report a novel smart actuator, which consists of polypyrrole and agar. Due to the water absorbing ability, the photothermal effect and the protonation behavior originating from polypyrrole and agar, the resulting actuator is responsive to a variety of stimuli, including humidity, light, temperature, HCl and NH3 gas. In addition, the actuator exhibits fast responsiveness, controllable actuation and large bending. Interestingly, two external stimuli can be combined to activate the actuator, leading to continuous shape changes in a programmable fashion. Multi-stimuli responsive actuators have opened up great opportunities in a variety of applications. As a proof-of-concept, we have demonstrated their application as a walking device with cargo transportation and delivery capabilities.
研究领域:
材料科学
·
仿生学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）