SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Yuxiao Hu,Xueying Chen,Zhijian Tan
Ammonium sulfate and t -butanol are widely used in conventional three-phase partitioning (TPP); however, their use is not eco-friendly. In this study, a novel TPP was developed using pH-responsive deep eutectic solvents (DESs) and sugars as substitutes for t -butanol and ammonium sulfate, respectively. This TPP was further used for the separation and purification of radish ( Raphanus sativus L.) peroxidase (POD). DES-3 (dodecanoic acid: decanoic acid = 1:1) and sucrose were selected as optimal phase-forming components. Single-factor experiments were performed to investigate the factors influencing the TPP process, and three major factors (sucrose concentration, extraction time, and extraction temperature) were optimized by response surface methodology (RSM). Under the optimized conditions, the POD recovery and purification fold reached 101.66% and 11.56, respectively. Cyclic experiments confirmed the retrievability of DESs. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the novel TPP had better purification efficiency than the traditional TPP. The method proposed in this study is more efficient than conventional TPP, and it does not require the use of organic solvents and high concentration salt solutions. Therefore, this method is promising in the application of TPP.
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Chemosensors
·
2023
·Vol. 11
·期 7
·398
·
IF 4.2
Pengjuan Ni,Siyuan Liu,Yizhong Lu
The threat of glyphosate to food safety has attracted widespread attention. Consequently, it is highly urgent to develop a sensitive and accurate method for glyphosate detection. Herein, a turn-on fluorescent method for glyphosate detection using polydopamine-polyethyleneimine (PDA-PEI) copolymer as a fluorescent probe andp-nitrophenylphosphate (PNPP)/alkaline phosphatase (ALP) as a fluorescence quenching system is developed. The PDA-PEI copolymer was prepared by a one-pot method under mild condition, and its fluorescence kept almost unchanged after storing in a refrigerator for one month. ALP catalyzed the hydrolysis of PNPP top-nitrophenol (PNP) that caused the fluorescence quenching of PDA-PEI copolymer via the inner filter effect. However, glyphosate inhibited ALP activity, thereby preventing the formation of PNP and restoring the fluorescence signal. Under the optimized conditions, the fluorescence of PDA-PEI copolymer depended on glyphosate concentrations ranging from 0.2 to 10 μg/mL with a detection limit of 0.06 μg/mL. Moreover, this assay was applied to detect glyphosate in real samples using the standard addition method. The recoveries were in the range from 88.8% to 107.0% with RSD less than 7.78%. This study provides a novel insight for glyphosate detection and expands the applications of fluorescent copolymer.Keywords:fluorescence;alkaline phosphatase;glyphosate;inner filter effect
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BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Hossain M. Zabed,Suely Akter,Mudasir A. Dar,Jamila A. Tuly,Mukesh Kumar Aswathi,Junhua Yun,Jia Li,Xianghui Qi
This study aimed to produce enhanced fermentable sugars from a novel stover system through the bioprocessing of its soluble sugars and insoluble carbohydrates. The pretreatment conditions were optimized for this high sugar-containing stover (HSS) to control inhibitor formation and obtain enhanced fermentable sugar concentrations. The optimum temperature, acid loading, and reaction time for the pretreatment were 155 °C, 0.5%, and 30 min, respectively, providing up to 97.15% sugar yield and 76.51 g/L total sugars at 10% solid-load. Sugar concentration further increased to 126.9 g/L at 20% solid-load, generating 3.89 g/L acetate, 0.92 g/L 5-hydroxymethyl furfural, 0.82 g/L furfural, and 3.75 g/L total phenolics as inhibitors. To determine the effects of soluble sugars in HSS on fermentable sugar yield and inhibitor formation, sugar-removed HSS was further studied under the optimum conditions. Although prior removal of sugars exhibited a reduction in inhibitor generation, it also decreased total fermentable sugar production to 115.45 g/L.
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CELLULOSE
·
2023
·Vol. 30
·期 12
·7575-7589
·
IF 5.7
Wang Tao,Zhang Cailiang,Feng Hui,Zou Qingjun,Huang Wenrui,Wu Jiacong,Han Juan,Wang Yun
In this study, a green and effective stepwise catalytic system for cellulose conversion to 5-hydroxymethylfurfural (HMF) is reported. In a pretreatment process, very fast (15 min) dissolution of cellulose was achieved at a low temperature of 70 °C, utilizing ZnBr 2 ·4H 2 O as the best dissolution solvent, which to improve the reactivity of cellulase for raw cellulose and increase efficiency of cellulose degradation. Enzyme-inorganic hybrid nanoflowers (Cellulase@β-glucosidase/Zn 3 (PO 4 ) 2 -NF and Glucose isomerase@Zn 3 (PO 4 ) 2 -NF) with different morphological structures were prepared to act as biocatalyst in the sequential conversion of pretreated cellulose to glucose and glucose to fructose. HCl was applied catalyst for dehydration of fructose to 5-HMF in a water/tert-butanol biphasic system. Under optimal reaction conditions, the yield of 5-HMF for the overall reaction from cellulose was about 21.6%. A feasibility route for green conversion of cellulose to 5-HMF is reported. Graphical abstract
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GREEN CHEMISTRY
·
2023
·Vol. 25
·期 16
·6297-6305
·
IF 9.8
Zhaohui Guo,Christian Marcus Pedersen,Honghong Chang,Yingxiong Wang,Yan Qiao
As a renewable source, fructose is of great significance in biomass conversion and food industries. Currently, most of the chemo-catalysts studied, with glucose as a substrate, are limited by the thermodynamic equilibrium of the reaction, resulting in the maximum yield of fructose of approximately 30–40%. The similar chemical structures and physical properties of monosaccharides bring another challenge for the separation of fructose from the reaction mixture. In this study, the combination of CaCl2 and Et3N was found to alter the thermodynamic equilibrium and promote fructose separation. The reaction parameters were optimized and up to 78% fructose yield could be obtained. The pathways of the isomerization reaction were found to involve both intramolecular hydride shift and an enediol intermediate mechanism, and the former was major. It could be speculated reasonably that Ca2+ played a key role in glucose conversion and interacted with glucose at its C1 and C2 positions, and Et3N interacted with Cl− while providing an alkaline environment. The formation of the α-fructofuranose’ complex, from Et3N, CaCl2 and α-fructofuranose, could shift the equilibrium of glucose isomerization towards fructose formation. According to the diffusion-ordered spectroscopy (DOSY) NMR results, the strong complexation slowed down the diffusion rate of the α-fructofuranose’ complex, allowing it to gradually precipitate, and hence drove the reaction equilibrium towards the desired fructose. The precipitate was filtered from the reaction mixture and processed further to obtain high-purity fructose in 45% isolated yield. Moreover, the filtrate containing solvent and reagents obtained in the separation process could be recycled.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Li Zheng,Xiaoqin Feng,Chaorui Zhang
The electrochemical oxidation behavior and oxidation mechanism of Basic Brown G (BBG) were studied at a carbon paste electrode (CPE) for the first time. In pH 11.0 phosphate buffer, two irreversible oxidation peaks of BBG at 0.39 V and 0.78 V (versus SCE) were observed, which were both two-electron and two-proton process with diffusion character. The two peaks were assigned to the oxidation of 1, and 1’-phenylamine groups and the further oxidation of the oxidation products in the BBG molecule. The diffusion coefficient of BBG was assessed to be 8.7×10 -5 cm 2 s -1 . A square wave voltammetric method was proposed for the determination of BBG. The peak current of the oxidation peak of BBG was linear with its concentration in the ranges from 0.004 to 0.2 μM and 0.2 to 2.0 μM, with a detection limit of 1.2 nM (3S/N). The proposed method was employed to the quantification of BBG in water samples.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2023
·
IF 3.8
Feiyan Wang,Linhong Liao,Xiaohui Liu,Jie Zhang,Fengshou Wu
Glucose is regarded as a clinical biomarker, and the abnormal level of glucose in blood would cause endocrine metabolic diseases. Thus, it is of great significance to develop a simple, accurate, and sensitive method for glucose detection. In this work, we synthesized an iron porphyrin-based porous organic framework (Fe-POF) through a facile alkylation reaction between 5,10,15,20-tetra(4-aminophenyl) porphyrin (TAPP) and cyanuric chloride. Fe-POF exhibited excellent peroxidase-like activity and could be used to detect hydrogen peroxide and the molecules associated with hydrogen peroxide such as glucose. The structure of Fe-POF was confirmed through a series of methods, such as FT-IR, SEM, EDS, TEM, and XPS. Fe-POF exhibited excellent peroxidase-like activity, which could catalyze the oxidization of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue product (ox-TMB) in the presence of hydrogen peroxide (H 2 O 2 ). Based on this phenomenon, a simple colorimetric assay was constructed for the detection of H 2 O 2 and glucose with high selectivity and sensitivity. The detection limit of H 2 O 2 and glucose was calculated to be 1.8 and 1.39 μM, respectively. Finally, Fe-POF was applied as a colorimetric sensor for rapid detection of glucose in diluted serum samples.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 15
·7631-7642
·
IF 4.1
Dongshuang Liang,Haosong Wen,Yaxuan Zhou,Taohong Wang,Guangqun Jia,Zongyan Cui,Adan Li
Background Honey aroma is one of its most important properties and it depends on the qualitative and quantitative composition of the volatile compounds. The volatile profile of honey could reveal its botanical origin to avoid a false characterization. Thus, it is of great significance to honey authentication. This study developed and validated a headspace solid-phase microextraction and gas chromatography–mass spectrometry (HS-SPME-GC–MS) method for simultaneous qualitative and quantitative analyses of 34 volatile components in honey. The developed method was applied to 86 honey samples from six different botanical origins, including linden honey, rape honey, jujube honey, vitex honey, lavender honey and acacia honey. Results The volatile fingerprints and quantitative results were simultaneously obtained by using the full scan and selected ion monitoring (SCAN+SIM) MS scanning mode. The limits of quantification (LOQs) and limits of detection (LODs) of 34 volatile compounds were in the ranges of 1–10 ng/g and 0.3–3 ng/g, respectively. And the spiked recoveries ranged between 70.6% and 126.2%, with the relative standard deviations (RSDs) not higher than 45.4%. A total of 98 volatile compounds were found with relative contents determined, and the 34 volatile compounds were determined with absolute concentrations. Based on the volatile fingerprints and the contents of volatile compounds, honey samples from six botanical origins were well classified by principal component analysis and orthogonal partial least-squares discrimination analysis. Conclusions The HS-SPME-GC–MS method was successfully applied to achieve the volatile fingerprints of six types of honey and to quantitatively analyze 34 volatile compounds with satisfying sensitivity and accuracy. Chemometrics analysis showed significant correlations between honey types and volatiles. These results reveal the characteristics of volatile compounds in six types of unifloral honey and provide some supports for honey authentication. © 2023 Society of Chemical Industry.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2023
·
IF 4
Qiao Tong,Jianjun Xi,Yu Cao,Ruoyu He,Yanmei Zhao,Yidan Shao,Xuwang Pan,Jinsong Huang,Yongping Chen,Rangxiao Zhuang
Chronic fructose consumption is becoming one leading risk factor for NAFLD due to its hazard on cellular lipid metabolism, which plays an essential role in the pathogenesis of NAFLD. Morroniside, an iridoid glycoside from Cornus officinalis, has been suggested to have potent in regulating cellular glucolipid metabolism. However, the effect and mechanism of morroniside on improving fructose-induced hepatic steatosis and retarding NAFLD progression remain ambiguous. In this study, we illustrate the efficacy of morroniside in alleviating fructose-triggered hepatic steatosis in mice. Mechanically, morroniside suppressed de novo lipogenesis and promoted β-oxidation by inhibiting the activation of PGC1β. Additionally, morroniside was found to work in attenuating hepatic inflammation in response to long-term fructose intake. Taken together, the current study reveals that morroniside is a promising food and medicinal therapy for NAFLD treatment and is effective in delaying the progression of NAFLD to NASH, especially in subtypes caused by excessive carbohydrate intake.
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INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Yunfei Song,Chengzhi Huang,Yuanfang Li
Vacancy engineering as an effective strategy has been widely employed to regulate the enzyme–mimic activity of nanomaterials by adjusting the surface, electronic structure, and creating more active sites. Herein, we purposed a facile and simple method to acquire transition metal manganese oxide rich in oxygen vacancies (OVs-Mn2O3-400) by pyrolyzing the precursor of the Mn(II)-based metal–organic gel directly. The as-prepared OVs-Mn2O3-400 exhibited superior oxidase-like activity as oxygen vacancies participated in the generation of O2•–. Besides, steady state kinetic constant (Km) and catalytic kinetic constant (Ea) suggested that OVs-Mn2O3-400 had a stronger affinity toward 3,3′,5,5′-tetramethylbenzidine and possessed prominent catalytic performance. By taking 2-phospho-l-ascorbic acid as the substrate, which can be converted into reducing substance ascorbic acid in the presence of alkaline phosphatase (ALP), OVs-Mn2O3-400 can be applied as an efficient nanozyme for ALP colorimetric analysis without the help of destructive H2O2. The colorimetric sensor established by OVs-Mn2O3-400 for ALP detection showed a good linearity from 0.1 to 12 U/L and a lower limit of detection of 0.054 U/L. Our work paves the way for designing enhanced enzyme-like activity nanozymes, which is of significance in biosensing.
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Food Bioscience
·
2023
·
IF 5.2
Shijin Xiong,Dandan Qiu,Xiaoyan Xu,Tonghao Du,Zhen Peng,Muyan Xiao,Tao Huang,Jinqing Huang,Hongbing Ren,Mingyong Xie,Tao Xiong
Laotan Suancai is a well-known Chinese traditional fermented vegetable with a unique flavor. However, there are significant differences in the quality of Laotan Suancai pickled by jars (LTSC-J) and pools (LTSC-P) on an industrial scale. To reveal the effect of fermentation craft on Laotan Suancai quality, the microbial community, flavor metabolites, and physicochemical properties of the two types of Laotan Suancai, as well as their interrelationship were investigated. LTSC-P had higher salinity, glucose content, and histidine content, while LTSC-J had higher TA, lactic acid content, and isobutyric acid content. Nine differential aroma-active compounds were found between the two types of Laotan Suancai: anethole , eucalyptol, diallyl sulfide, estragole, diallyl disulfide , eugenol, linalool, nonanal , and ethyl butyrate. LTSC-P showed a higher relative abundance of Weissella , Acinetobacter , Pseudomonas , Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium , Inhella , and Leuconostoc compared to LTSC-J. Co-occurrence analysis indicated the distinct differences in the co-occurrence patterns of the microbial network across the two types of Laotan Suancai. RDA analysis indicated that glucose, salinity, TA, maltose , and nitrite were the main drivers for the differences in bacterial communities between the two types of Laotan Suancai. Spearman correlation analysis revealed strong correlations between 18 bacteria and 61 flavor compounds. Finally, bacterial metabolic function prediction indicated that sulfide and aromatic ester may be biosynthesized by sulfur metabolism , cysteine and methionine metabolism, pyruvate metabolism, and butanoate metabolism. Overall, these findings provide important guidance for improving the manufacturing and quality of Laotan Suancai.
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LANGMUIR
·
2023
·
IF 3.9
Gong Zhang,Qing Li,Jieai Li,Menghan Pan,Yuefei Wang,Rongxin Su,Wei Qi,Wei Zhang
The development of stable multifunctional enzyme mimics with tandem catalytic effects provides a great opportunity to construct economical and convenient bioassays. Inspired by biomineralization, in this work self-assembled N-(9-fluorenylmethoxycarbonyl)-protected tripeptide (Fmoc-FWK-NH2) liquid crystals were used as templates to in situ mineralize Au nanoparticles (AuNPs), and then a dual-functional enzyme-mimicking membrane reactor based on AuNPs and peptide-based hybrids was constructed. AuNPs with a uniform particle size and good dispersion were in situ reduced on the surface of the peptide liquid crystal due to the reduction of the indole group on the tryptophan residue, which exhibited excellent peroxidase-like and glucose oxidase-like activities simultaneously. Meanwhile, the oriented nanofibers aggregated into a three-dimensional network, which was further immobilized on the mixed cellulose membrane to form a membrane reactor. A biosensor was made to realize fast, low-cost, and automatic detection for glucose. This work represents a promising platform for the design and construction of novel multifunctional materials based on the biomineralization strategy.
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