FOOD CHEMISTRY
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2024
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IF 8.5
Qing Zhang,Yujin Yang,Yannan Chen,Shuoshuo Ban,Shaobin Gu,Fang Li,Mengmeng Xue,Jianrui Sun,Xin Li,Shanshan Tie
The purpose of this study was to prepare a pH-responsive microgel for co-delivering Weizmannia coagulans 99 (BC99) and procyanidins (PCs) to enhance the survival rate and tolerance of probiotics in complex micro-environment. The effects of different concentrations of PCs on the properties of microgels were optimized, and found that the spherical microgels had higher encapsulation efficiency (90.27 ± 2.51%) and smaller size when the concentration of PCs was 20 μg/mL. The interaction among PCs, pectin and protein could effectively improve the survival rate of BC99 under different pH, bile salt, digestive enzyme and temperature conditions, maintain their stability in acidic gastric fluid, and realize the release of probiotics in neutral intestinal fluid. Moreover, the microgel was able to protect BC99 against H 2 O 2 and antibiotics. This work provides a pH-responsive co-loaded microgels for BC99 and PCs, and has the potential in the loading and delivery of other probiotics and polyphenols.
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BIOMACROMOLECULES
·
2024
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IF 5.5
Shuqi Chen,Wenxia Gao,Pengjin Ge,Shuhua Chang,Ting Wang,Quan Zhao,Bin He
Ulcerative colitis (UC), a chronic inflammatory bowel disease, poses a heightened colorectal cancer risk due to persistent mucosal inflammation and barrier dysfunction. In this article, a negatively charged thermosensitive hydrogel loaded with pectin microspheres was used as the enema for UC treatment. Succinic acid was immobilized on poly(ε-caprolactone-co-glycolide)-poly(ethylene glycol)-poly(ε-caprolactone-co-glycolide) (PCLGA-PEG-PCLGA) triblock copolymers to preferentially coat on cationic-inflamed sites via electrostatic interaction for reconstructing the mucosal barrier. Anti-inflammation drug 5-aminosalicylic acid (5-ASA) and curcumin-loaded pectin microspheres (Pec@Cur) were dispersed in the hydrogel for the inflammatory treatment of UC. The thermally sensitive hydrogels were rectally injected into UC model mice. The hydrogel effectively adhered to ulcers and prolonged colon retention, enabling sustained drug release and remarkably relieving the symptoms of colitis. The negatively charged hydrogel exhibited excellent significance in the UC treatment.
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ACS Applied Nano Materials
·
2024
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IF 5.3
Zhiyuan Meng,Qinchao Wu,Xinyi Wu,Chunmei Yang,Wangjin Xu,Tingting Lin,You Liang,Xiaojun Chen
The enhancement of pesticide utilization efficiency for improved biological activity, while promoting crop growth to enhance disease resistance, holds significant importance in agricultural production. In this study, we developed an enzyme-responsive azoxystrobin (AZOX) delivery system using iron-based mesoporous materials as porous carriers and pectin (Pec) as an encapsulation agent to manage rice sheath blight. The resultant AZOX-AFS-Pec nanoparticles (NPs) exhibit high loading capacity and could intelligently release AZOX in acidic conditions in the presence of pectinase. Notably, AZOX-AFS-Pec NPs demonstrated improved wetting and adhesion properties on rice blades, thereby augmenting AZOX utilization. Furthermore, AZOX-AFS-Pec NPs enhanced fungicidal activity against rice sheath blight, with pot experiments revealing comparable control efficacy on rice sheath blight to AZOX suspension concentration (SC). Specifically, AZOX-AFS-Pec NPs exhibited superior prevention and prolonged protection against rice sheath blight compared to AZOX SC. Additionally, continuous release of Fe2+ and Fe3+ by AZOX-AFS-Pec NPs increased chlorophyll content and promoted rice growth. Moreover, these NPs increased salicylic acid (SA) levels in rice plants, fortifying disease resistance. Importantly, AZOX-AFS-Pec NPs demonstrated over a 2-fold reduction in acute toxicity to earthworms compared to AZOX SC. Therefore, stimuli-responsive AZOX-AFS-Pec NPs show great promise for enhancing pesticide effectiveness against pathogens, improving crop traits, and fortifying disease resistance.
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FOOD CHEMISTRY
·
2018
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IF 4.946
Xiao-Yan Dai,Li-Min Kong,Xiao-Ling Wang,Qing Zhu,Kai Chen,Tao Zhou
Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N , N ′-dicyclohexylcarbodiimide/ N -hydroxysuccinimide as the activating agent. The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses. Immobilization conditions were optimized by Box–Behnken design and the response surface method. The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%. The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0. The optimal temperature of immobilized pectinase was increased to 60 °C, which was 10 °C higher than that of free form. Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase. Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles. Due to these good properties, such immobilized pectinase may find application in food industry.
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RSC Advances
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2018
·Vol. 8
·期 18
·9762-9769
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IF 2.936
Chen Li,Yun-shan Wei,Peng Wen,Kun Feng,Min-hua Zong,Hong Wu
A novel electrospun colon-specific delivery system for salmon calcitonin (SCT) was developed to improve its stability and bioavailability. Firstly, the pectin-coated SCT liposomes were prepared by film dispersion method and then a liposomes/sodium alginate/polyvinyl alcohol fiber mat was fabricated by electrospining. Scanning electron microscopy analysis indicated that the obtained nanofibers were uniform and smooth with an average diameter of about 350 nm. The release of SCT in different simulated digestive fluids was studied and corresponding release kinetics models were built. It was found that the fiber mat containing pectin-coated SCT liposomes had better stability and colon-specific properties compared with that containing uncoated SCT liposomes and the release of SCT in the colon followed the case II transport mechanism. In addition, there is no significant change in the bioactivity of released SCT measured by ELISA. This study shows that the electrospun colon-specific fiber mat is a potential delivery system for bioactive peptides.
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Analytical Methods
·
2025
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IF 2.6
Chen Lu,Jiayue Chen,Shengsheng Zhang,Yaru Tian,Xinyu Ying,Dan Zhang,Zhidan Luo,Xinxin Si,Ming Li
Two single nucleotide polymorphisms (SNPs) in the human SLCO1B1 gene, c.388A>G (rs2306283) and c.521T>C (rs4149056), are independent determinants of the efficacy and side effects of statin drugs. Multinational clinical guidelines recommend testing for SLCO1B1 genotypes before the initial use of statins. Current SLCO1B1 SNP identification methods, primarily based on quantitative fluorescence PCR, rely on expensive equipment, are time-consuming, and require cold-chain storage for reagents, making them unsuitable for use in resource-limited healthcare settings. In this study, we developed a CRISPR/Cas12b-based amplification-free genotyping technique for SLCO1B1 SNPs. Within 30 minutes of the isothermal reaction, genotyping of the c.388A>G and c.521T>C SNPs in the SLCO1B1 gene can be observed by the naked eyes under blue light. Additionally, maltodextrin was identified as an effective vitrification stabilizer for the CRISPR/Cas12b premix. A low-cost vitrification process was optimized to prepare a glass like solid reagent via room-temperature vacuum drying. The vitrified CRISPR/Cas12b reagent retained approximately 88% of its activity after 30 days of storage at 37 °C, eliminating the need for cold-chain storage and allowing for long-term preservation at room temperature. This vitrified CRISPR/Cas12b based rapid SNP detection technique is especially suitable for genotyping drug metabolism genes in primary healthcare settings, providing effective guidance for precision medicine in clinical practice.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Huan Guo,Wenyi Ran,Xuchu Jin,Yina Huang,Feiwu Long,Yue Xiao,Ren-You Gan,Yanping Wu,Hong Gao
Accidents and surgical procedures inevitably lead to wounds, presenting clinical challenges such as inflammation and microbial infections that impede the wound-healing process. This study aimed to address these challenges by developing a series of novel wound dressings known as electrospun biomimetic nanofiber membranes. These membranes were prepared using electrostatic spinning technique, incorporating hydroxypropyl-β-cyclodextrin/dihydromyricetin inclusion complexes. The prepared electrospun biomimetic nanofiber membranes exhibited randomly arranged fiber morphology with average fiber diameters ranging from 200 to 400 nm, resembling the collagen fibers in the native skin. These membranes demonstrated excellent biocompatibility, hemocompatibility, surface hydrophilicity, and wettability, while also releasing dihydromyricetin in a sustained manner. In vitro testing revealed that these membranes, loaded with hydroxypropyl-β-cyclodextrin/dihydromyricetin inclusion complexes, displayed higher antioxidant potential and inhibitory effects against Staphylococcus aureus and Escherichia coli . Furthermore, these membranes significantly reduced the M1 phenotypic transition in RAW264.7 cells, even when stimulated by lipopolysaccharides, effectively restoring M2 polarization, thereby shortening the inflammatory period. Additionally, the in vivo wound healing effects of these membranes were validated. In conclusion, this study introduces a promising nanofiber membrane with diverse biological properties that holds promise for addressing various crucial aspects of the wound-healing process.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Xiquan Li,Xinhua Zhang,Jiale Lv,Xiuling Zhang,Yingying Li,Xiaofeng Han,Wentao Zhang
In current study, curcumin-loaded bioactive nanocomplexes (Cur NCs) (2 %, 5 %, 8 %, and 11 %) were used to prepare corn starch (CS)-based composite films (CS-Cur NCs). Fourier-transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy revealed that Cur NCs were uniformly dispersed in the polymer matrix via physical interaction. Moreover, the mechanical, gas barrier, hydrophobicity, optical, and thermal properties and the antioxidant activity of composite films were potentially improved with the addition of Cur NCs. Subsequently, CS-based film with 11 % Cur NCs exhibited high antioxidant activity (the scavenging rates of DPPH and ABTS are 50.07 % ± 0.82 % and 65.26 % ± 1.60 %, respectively) and was used for packaging blueberries. Compared with the control, the CS-Cur NCs packaging treatment effectively improved the appearance and nutrition of blueberries, and maintained the high activity of several antioxidant enzymes. Furthermore, CS-Cur NCs packaging treatment significantly improved the ascorbic acid (AsA) and glutathione (GSH) levels, thus regulating the AsA-GSH cycle system and suppressing the accumulation of reactive oxygen species (ROS). In summary, the CS-Cur NCs packaging could effectively conserve the postharvest quality of blueberries by improving antioxidant enzyme activity and suppressing excessive accumulation of ROS, which contributes to the development of bioactive packaging and provides novel insights into the preservation of blueberries. This work demonstrates that the development of active packaging is promising to absorb the oxidative radicals from food, and protect the food from inherent and external factors, thus enhancing the quality, security, and shelf-life of the food during storage.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Hujun Xie,Xin Luo,Ying Gao,Min Huang,Gerui Ren,Rongmi Zhou,Yulu Sun,Haoxin Ye,Qunfang Lei,Wenjun Fang,Yong-Quan Xu
Probiotics and epigallocatechin gallate (EGCG) are sensitive to external conditions. In order to improve the survival rate of Lactobacillus plantarum (LP) and the activity of EGCG during storage, freeze-drying and gastrointestinal digestion, the LP and EGCG are co-encapsulated for the first time by extrusion using alginate-whey protein isolate (ALG-WPI) and alginate-pectin-whey protein isolate (ALG-PEC-WPI) biocomposites as wall materials, which can protect the encapsulated LP and EGCG during the simulated gastric digestion and co-release LP and EGCG in the intestine. Compared with ALG-PEC-WPI-LP beads, the release rate of LP in ALG-PEC-WPI-LP-EGCG beads is significantly increased to 92.8%. Both co-encapsulated beads are formed through hydrogen bonding interaction, and EGCG exists in amorphous form. Two co-encapsulated beads ALG-WPI-LP-EGCG and ALG-PEC-WPI-LP-EGCG exhibit better survival rate for LP during freeze-drying and in vitro digestion. Experiments conducted in high-fat diet-fed rats indicate that serum total cholesterols and low-density lipoprotein cholesterols in co-encapsulated group are significantly lower than those of un-encapsulated group. The co-encapsulated LP and EGCG alleviate the gut dysbiosis and decrease the hepatic steatosis in high-fat diet-fed rats. Thus the ALG-PEC-WPI composite is a good carrier for protection and delivery of LP and EGCG.
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Journal of Materials Research and Technology-JMR&T
·
2024
·
IF 6.2
Hao Zhang,Yufei Tang,Xuan Zhou,Qian Liang,Yani Sun,Bo Zhang,Kang Zhao,Zixiang Wu
The development of biodegradable bone scaffolds that align with the growth cycle of new bone has become a prominent focus in the field of bone defect repair. The sintered porous hydroxyapatite scaffolds exhibit a high degree of crystallization and demonstrate a slow degradation rate following implantation. The apatite phase with low crystallinity can be obtained following the hydration reaction of the non-sintered calcium phosphate bone cement (CPC) scaffolds. However, this leads to the formation of a weak alkaline environment during degradation, ultimately resulting in unsustainable degradation. The present study focuses on the fabrication of porous pectin/CPC composite scaffolds through two approaches during the preparation process of non-sintered porous CPC scaffolds: uniform composite and surface modification. The degradation performance of porous composite scaffolds exhibited an increase with the rise in pectin content. At a pectin content of 70 mg/mL, the scaffolds demonstrated a degradation rate of 13.79% within a span of 30 days. The mechanical properties of the porous scaffolds were enhanced with an increase in pectin concentration through surface coating. When the pectin concentration was 5 wt%, the scaffold exhibited a compressive strength of 8.72 MPa, an elastic modulus of 0.82 GPa, and experienced a degradation rate of 12.57% after a period of 30 days. The local acidic environment not only facilitates the dissolution of bone-like apatite but also enhances cell proliferation and adhesion. Pectin/apatite porous scaffolds exhibit excellent biocompatibility, offering a novel approach for the development of biodegradable bone replacement scaffolds.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Changqing Miao,Qiqi Song,Rui Fu,Xin Yang,Jie Gu,Yaxiong Wang,Ruze Liang,Jili Wang,Huazheng Sai
How to solve the contradiction between the efficiency and adsorption rate of porous materials in adsorbing pollutants has always been one of the focus issues. In this study, the small landscape cypress trees structure like biomimetic of a hierarchical and dual morphology 3D porous HA-based aerogel was designed and synthesized to use humic acid (HA), pectin (PE) and chitosan (CTS) as raw materials, which it was formed by the disorderly overlapping of lamella composed of fiber networks in 3D space. Due to its special microstructure, it can be used like separation membrane, which allowing for rapid adsorption of pollutants in the water while the water flow passes through quick. In general, this work provides a new concept for owning fast adsorption rate and efficient adsorption of porous materials of preparation to use green method.
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BIOMATERIALS
·
2024
·
IF 12.8
Dan Wang,Wenqi Ma,Yuanyuan Zhang,Yufeng Wang,Lei Sun,Jue Jiang,Lianying Jiao,Runqing Li,Yujie Zhang,Mingzhen Zhang,Qi Zhou
Thyroid cancer is increasing globally, with anaplastic thyroid carcinoma (ATC) being the most aggressive type and having a poor prognosis. Current clinical treatments for thyroid cancer present numerous challenges, including invasiveness and the necessity of lifelong medication. Furthermore, a significant portion of patients with ATC experience cancer recurrence and metastasis. To overcome this dilemma, we developed a pH-responsive biomimetic nanocarrier (CLP@HP-A) through the incorporation of Chlorin e6 (Ce6) and Lenvatinib (Len) within hollow polydopamine nanoparticles (HP) that were further modified with platinum nanoparticles (Pt), enabling synergistic chemotherapy and sonodynamic therapy. The CLP@HP-A nanocarriers exhibited specific binding with galectin-3 receptors, facilitating their internalization through receptor-mediated endocytosis for targeted drug delivery. Upon exposure to ultrasound (US) irradiation, Ce6 rapidly generated reactive oxygen species (ROS) to induce significant oxidative stress and trigger apoptosis in tumor cells. Additionally, Pt not only alleviated tumor hypoxia by catalyzing the conversion of H 2 O 2 to oxygen (O 2 ) but also augmented intracellular ROS levels through the production of hydroxyl radicals (•OH), thereby enhancing the efficacy of sonodynamic therapy. Moreover, Len demonstrated a potent cytotoxic effect on thyroid cancer cells through the induction of apoptosis. Transcriptomics analysis findings additionally corroborated that CLP@HP-A effectively triggered cancer cell apoptosis, thereby serving as a crucial mechanism for its cytotoxic effects. In conclusion, the integration of sonodynamic/chemo combination therapy with targeted drug delivery systems offers a novel approach to the management of malignant tumors.
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