Bioactive Carbohydrates and Dietary Fibre
·
2020
·
IF 0
Jinxia Meng,Zhouya Bai,Wenqi Huang,Ying Liu,Peilin Wang,Shaoping Nie,Xiaojun Huang
Type 2 diabetes (T2D), as the most common type of diabetes, has become the public problem in the world for increasing prevalence. In this study, the effects of polysaccharide from white kidney bean (WKBP) on streptozotocin-induced diabetic rats were investigated. Administration of WKBP decreased the levels of blood glucose , glycated serum protein and increased the level of insulin of diabetic rats. Treatment of WKBP reduced the concentration of total cholesterol and raised the ratio of high and low-density lipoprotein cholesterol related to serum lipids . WKBP also can lessen damage of pancreas, kidney and liver, and contribute to their function restoration. The results found that WKBP exhibited high potential on helping with hyperglycemia , hyperlipidemia and alleviating the damage of pancreas, kidney and liver of T2D.
研究领域:
生物化学
·
药理学
·
内分泌学
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DOI
Journal of Functional Foods
·
2024
·
IF 3.8
Caixia Li,Yaping Liu,Mingzhi Yang,Haoyue Huang,Lulu Tang,Yufan Miao,Wenjie Li,Xing Li
It has been reported that fucoidan plays a beneficial role in type 2 diabetic mellitus (T2DM). However, it remains obscure if fucoidan has the protective effects on diabetes induced skeletal muscle atrophy. Here, we investigated the effect and mechanism of fucoidan on diabetic muscle atrophy. The results revealed that fucoidan ameliorated the general symptoms of diabetes, alleviated insulin resistance, as well as improved glucose metabolism disorders in vivo and vitro . Moreover, fucoidan also improved the atrophy of skeletal muscle by resisting the reduction of muscle fiber diameter and balance the protein anabolism and catabolism. It is worth noting that these beneficial effects of fucoidan on skeletal muscle atrophy and insulin resistance were abolished by inhibiting PI3K/Akt pathway. Taken together, fucoidan ameliorates diabetic skeletal muscle atrophy at least partially by the PI3K/Akt pathway.
研究领域:
药理学
·
内分泌学
·
肌肉生物学
✓ 已验证
DOI
高影响因子
Energy Science & Engineering
·
2025
·
IF 3.4
Zhangyang Li,Chunling Wang,Daoping He,Fangming Jin
Photocatalytic biomass valorization toward multicarbon chemicals holds promise for sustainable energy solutions. Nevertheless, this approach suffers from dual challenges: inherent instability of polyhydroxy intermediates and inefficient formic acid production under ambient conditions. Here, we demonstrated that a TiO 2-x photocatalyst with oxygen vacancies can effectively catalyze the transformation of glucose into arabinose, erythrose, and formic acid using O 2 as an oxidant in pure water under natural sunlight irradiation. The C 1 −OH group of glucose adsorbed on the oxygen vacancies of TiO 2-x undergoes photo-induced hole oxidation, leading to the formation of gluconolactone-TiO 2-x as a new motif. Subsequent cleavage of bonds by ·OOH radicals generated from oxygen reduction produces arabinose, erythrose, and formic acid. The oxygen vacancy strategy achieves a remarkable 53.9% yield for glucose oxidation, which exhibits 10.8-fold enhancement compared to that of conventional TiO 2 without oxygen vacancies, thereby opening new avenues for developing efficient photocatalytic systems for sustainable biomass valorization.
研究领域:
光催化
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生物质转化
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材料科学
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环境化学
·
可持续能源
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yucheng Yan,Safdar Ali Amur,Hong Liu,Ruoyao Shen,Huaiqing Sun,Yunlin Pei,Chaowan Guo,Hao Liang
With the extensive application of baicalein in the treatment of cardiovascular and cerebrovascular diseases, its clinical and market demand has gradually expanded. But the natural yield of baicalein is very low, and it is mainly prepared by the deglycosylation of baicalin. However, the insolubility of baicalin in water significantly limits the deglycosylation of it under biocatalysis. To make biocatalysis of baicalin more efficient and environmental, a strategy was designed to enhance its water solubility through the solubilization mechanism of endogenous biological macromolecules, and the effect on the activity of glucuronidase was further explored. The results showed that wrapping with Scutellaria baicalensis polysaccharide (SBP) significantly improved the solubility of baicalin in water (the water solubility of baicalin increased by 23 times, BI/SBP = 1/12, w/w ). It was not only contributed to the efficient production of baicalein by one-pot method, but also effectively improved the deglycosylation rate of baicalin (increase by 47.04 % in aqueous solution). With the help of the solubilization of endogenous polysaccharide on baicalin in aqueous solution, a green, low-cost and efficient method (one-pot method) was designed to simultaneously extract and enzymatic hydrolyze baicalin to prepare baicalein. Under the same conditions, the yield of one-pot method is 87.17 %, which was much higher than that of the conventional method (29.38 %). In addition, one-pot method with the aid of endogenous polysaccharide could simply and conveniently prepare aglycone of other insoluble natural flavonoids, which has a wide range of industrial application value.
研究领域:
药理学
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生物技术
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化学工程
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产品关联
DOI
PubMed
高影响因子
ChemistrySelect
·
2024
·Vol. 9
·期 11
·e202400114
·
IF 1.9
Jin Liang,Xiaoxin Wang,Dongsheng Yu,XiTao Luo,Xiangfeng Liang,Zhongliang Su,Qinghui Shou
Graphical Self-supported osmotic membranes were prepared by blending PIM-1-mPEG, which was obtained by modifying PIM-1 with polyethylene glycol monomethyl ether (mPEG), and hydrophilic Tröger's base polymer (DMBP-TB). The membranes utilized microporous molecular sieves and the differences in solute-membrane interactions to achieve high pentose/hexose selectivity in aqueous solutions. Monosaccharides are vital building blocks in bioengineering applications; however, their extraction from intricate mixtures is challenging and uses substantial amounts of energy. Polymers of intrinsic microporosity (PIMs) offer an innovative avenue for separating monosaccharides. We modified PIM-1membranes to improve the glucose/xylose separation by incorporating polyethylene glycol monomethyl ether (mPEG). The optimal mPEG (molecular weight: 1000 Da; mass fraction: 2.5 %; solvent: methanol) delivered a xylose separation coefficient of 2.62. With the hybrid membrane of PIM-1-mPEG (50 w.t.%) and hydrophilic Tröger's base polymerer (DMBP-TB, 50 w.t.%), the separation factor for xylose/glucose in an aqueous solution was 2.51 for single-stage running and 11.32 after five-stage running. There are large fractions of micropores for PIM-1-mPEG, and there is difference on solute-membrane interactions for pentose/hexose, which are regarded to be the main driving force for the high pentose/hexose selectivity in methanol. The blending of PIM-1-mPEG and DMBP-TB, integrates the microporosity and hydrophilicity, finally endues the high pentose/hexose selectivity in aqueous solution. These microporous membranes are promising materials for efficiently separating monosaccharides and jnl> small organic molecules while minimizing energy consumption. We established a solid foundation for further exploring microporous membranes for various applications, notably in bioengineering.
研究领域:
生物工程
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材料科学
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分离技术
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产品关联
DOI
高影响因子
Microbial Biotechnology
·
2024
·
IF 4.8
Mengmeng Wu,Zhuangzhuang Shi,Yue Ming,Yufei Zhao,Ge Gao,Guoqiang Li,Ting Ma
Corn straw is an abundant and renewable alternative for microbial biopolymer production. In this paper, an engineered Sphingomonas sanxanigenens NXG -P 916 capable of co-utilising glucose and xylose from corn straw total hydrolysate to produce xanthan gum was constructed. This strain was obtained by introducing the xanthan gum synthetic operon gum as a module into the genome of the constructed chassis strain NXdPE that could mass produce activated precursors of polysaccharide, and in which the transcriptional levels of gum genes were optimised by screening for a more appropriate promoter, P 916 . As a result, strain NXG -P 916 produced 9.48 ± 0.34 g of xanthan gum per kg of fermentation broth (g/kg) when glucose was used as a carbon source, which was 2.1 times improved over the original engineering strain NXdPE:: gum . Furthermore, in batch fermentation, 12.72 ± 0.75 g/kg xanthan gum was produced from the corn straw total hydrolysate containing both glucose and xylose, and the producing xanthan gum showed an ultrahigh molecular weight (UHMW) of 6.04 × 10 7 Da, which was increased by 15.8 times. Therefore, the great potential of producing UHMW xanthan gum by Sphingomonas sanxanigenens was proved, and the chassis NXdPE has the prospect of becoming an attractive platform organism producing polysaccharides derived from biomass hydrolysates.
研究领域:
生物技术
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微生物学
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生物工程
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可再生资源
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 10
·1573
·
IF 4.7
Shufen Wu,Changyan Dong,Meihui Zhang,Yi Cheng,Xiaobo Cao,Benxu Yang,Chao Li,Xin Peng
Red yeast rice dietary supplements have been proven to ameliorate hyperglycemia, but the mechanism was unclear. In this work, ankaflavin (AK) and monascin (MS), as typical pigments derived from red yeast rice, were found to exert noteworthy inhibitory ability against α-glucosidase, with an IC50of 126.5 ± 2.5 and 302.6 ± 2.5 μM, respectively, compared with acarbose (IC50= 341.3 ± 13.6 μM). They also exhibited mixed-type inhibition of α-glucosidase in vitro and caused fluorescence quenching through the static-quenching process. Molecular-docking studies indicated that AK and MS bind to amino acid residues outside the catalytic center, which induces structural changes in the enzyme, thus influencing its catalytic activity. The anti-glycation ability ofMonascus-fermented products was evaluated, and they exhibited a high inhibition rate of 87.1% in fluorescent advanced glycation end-product formation at a concentration of 0.2 mg mL−1, while aminoguanidine showed a rate of 75.7% at the same concentration. These results will be significant in broadening the application scope ofMonascuspigments, especially AK and MS, in treating type 2 diabetes.
研究领域:
药理学
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营养学
·
糖尿病研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Wanxian Wang,Yanfang Liu,Dian Liu,Han Zhou,Yan Li,Wenjie Yuan,Suowen Xu,Jixia Wang,Xinmiao Liang,Jianping Weng
Pueraria lobata (Willd.) Ohwi, known as kudzu and used as a “longevity powder” in China, is an edible plant which is rich in flavonoids and believed to be useful for regulating blood sugar and treating diabetes, although the modes of action are unknown. Here, a total of 53 flavonoids including 6 novel compounds were isolated from kudzu using multidimensional preparative liquid chromatography. The flavonoid components were found to lower blood sugar levels, promote urine sugar levels in mice, and reduce the urine volume. Molecular docking and in vitro assays suggested that the antidiabetic effect of kudzu was attributed to at least three targets: sodium-dependent glucose transporter 2 (SGLT2), protein tyrosine phosphatase-1B (PTP1B), and alpha-glucosidase (AG). This study suggests a possible mechanism for the antidiabetic effect that may involve the synergistic action of multiple active compounds from kudzu.
研究领域:
药理学
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生物化学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2013
·Vol. 8
·期 8
·e70841
·
IF 3.73
Qian Shang,Junfeng Xiang,Hong Zhang,Qian Li,Yalin Tang
One of the most important carbohydrate-splitting enzymes is themaltase-glucoamylase which catalyzes the hydrolysis of alpha-glucosidic linkages. Maltase-glucoamylase inhibitors during the last few years have aroused medical interests in the treatment of diabetes. They contribute to a better understanding of the mechanism of maltase-glucoamylase. At present there are many different classes of maltase-glucoamylase inhibitors. This paper focuses on alkaloidal inhibitors of maltase-glucoamylase and structure-activity relationship (SAR) studies between them in order to discover some drugs with better efficiency and lower toxicity for treating diabetes.
研究领域:
药理学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Chuanqi Sun,Miaozi Lou,Zonglin Li,Feiyan Cheng,Zhimin Li
Nucleoside disaccharides are essential glycosides that naturally occur in specific living organisms. This study developed an enhanced UDP-glucose regeneration system to facilitate the in vitro multienzyme synthesis of nucleoside disaccharides by integrating it with nucleoside-specific glycosyltransferases. The system utilizes maltodextrin and polyphosphate as cost-effective substrates for UDP-glucose supply, catalyzed by α-glucan phosphorylase (αGP) and UDP-glucose pyrophosphorylase (UGP). To address the low activity of known polyphosphate kinases (PPKs) in the UDP phosphorylation reaction, a sequence-driven screening identified RhPPK with high activity against UDP (>1000 U/mg). Computational design further led to the creation of a double mutant with a 2566-fold increase in thermostability at 50 °C. The enhanced UDP-glucose regeneration system increased the production rate of nucleoside disaccharide synthesis by 25-fold. In addition, our UDP-glucose regeneration system is expected to be applied to other glycosyl transfer reactions.
研究领域:
生物化学
·
合成生物学
✓ 已验证
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Yubo Zhou,Shuo Tang,Ying Lv,Daihui Zhang,Xiaode Huang,Yanan Chen,Chenhuan Lai,Qiang Yong
To explore the impacts of galactose side-chain on the prebiotic activity of xyloglucan oligosaccharides (XGOS), XGOS and de-galactosylated XGOS (DG-XGOS) were prepared from tamarind using an enzymatic method. The differences in structural features of XGOS and DG-XGOS were systematically analyzed. Their in vitro fermentation characteristics of human fecal microbiota were explored. These results indicated that both XGOS and DG-XGOS promoted short-chain fatty acids (SCFAs) production, decreased pH, and changed the microbiota composition of the fermentation broth. Comparatively, DG-XGOS was more effective than XGOS in producing SCFAs, inhibiting the phylum Proteobacteria prevalence, and promoting the phyla Bacteroidetes and Actinobacteria prevalence. In summary, the xyloglucan degradation products exert potential prebiotic activity. Removing the galactose side-chains further enhances oligosaccharide utilization by fecal microbiota, offering a valuable approach to improve the biological efficacy of oligosaccharides.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Doudou Ma,Chuanhui Zhang,Yilong Wei,Li Ma,Xiaoliang Zhao,Weijie Zhang
Type 2 diabetes (T2D) is a chronic metabolic disorder characterised by hyperglycaemia, insulin resistance, and relative insulin deficiency. Insulin resistance results in patients requiring increased insulin injections and its limited bioavailability. The present subject matter aims to use Pueraria lobata polysaccharides, which can alleviate insulin resistance and have hypoglycaemic potency, as a medicinal excipient to prepare a hydrogel with excellent slow-release properties, which can be used to load insulin for injection or as a diabetic wound dressing material. Pueraria lobata polysaccharides (PLR) were extracted by hydroalcohol precipitation, and oxidised Pueraria lobata polysaccharides (O-PLR) were successfully prepared by sodium periodate oxidation and verified by structural characterisation. Subsequently, double crosslinked hydrogels were prepared using oxidised Pueraria lobata polysaccharides, chitosan and cellulose acetoacetate, and several of their properties were investigated in detail. The experimental results showed that the O-PLR double-crosslinked hydrogels possessed good rheological properties and injectability, as well as excellent self-healing and antimicrobial activities; moreover, the O-PLR hydrogels exhibited significant hypoglycemic activity, which is expected to be an important material for the treatment of Type 2 diabetes mellitus. Overall, this study provides a reliable theoretical and experimental basis for the development of hydrogels with hypoglycaemic, pro-healing and antibacterial functions.
研究领域:
药学
·
生物材料学
·
糖尿病研究
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）