Journal of Food Measurement and Characterization
·
2022
·Vol. 16
·期 5
·3712-3721
·
IF 3.006
Wang Tao,Wang Mengnan,Gao Yuan,Hu Xuelian,Wang Haiyang,Fang Guozhen,Wang Shuo
Development of materials with green and efficient for restraining the growth of the harmful microorganisms is vital for food safety. Herein, we constructed nanoliposomes with cinnamaldehyde (CIN), an active component of natural cinnamon essential oil, for the long-term inhibition of microbes in food spoilage. In this strategy, cinnamaldehyde nanoliposomes (CIN-NLs) were prepared by the method of thin film dispersion, and then decorated with ε-polylysine (ε-PL) to obtain ε-polylysine/cinnamaldehyde nanoliposomes (ε-PL/CIN-NLs). Results identified that CIN-NLs and ε-PL/CIN-NLs were in spherical and nanoscale, and the nanoliposomes had larger particle size distribution (122.5 ± 3.51–680.4 ± 7.40 nm) and higher encapsulation efficiency (19.4 ± 0.3–30.4 ± 0.2%) after ε-PL (2 mg/mL) decoration. When the CIN concentration was 4 mg/mL, the highest encapsulation efficiency was acquired as 30.4 ± 0.2% by ε-PL/CIN-NLs. CIN-NLs and ε-PL/CIN-NLs showed great activity on inhibiting the growth of Aspergillus flavus and Penicillium citreo-viride , and the inhibition zone diameter of ε-PL/CIN-NLs (26 ± 1.1 mm and 45 ± 1.7 mm, respectively) was significantly larger than that of CIN-NLs (20 ± 1.5 mm and 35 ± 2.1 mm, respectively) in final growth of two fungi. The studies indicated that that ε-PL decoration effectively improved the long-term antifungal activity characteristics of CIN nanoliposomes. Findings from current study can provide a new method to prevent food spoilage and direct future development of green antifungal packing for use in food preservation. Graphical abstract The schematic representation of ε-PL/CIN-NLs and the colony growth images of Aspergillus niger and Penicillium citreo-viride.
研究领域:
食品科学
·
微生物学
·
材料科学
✓ 已验证
DOI
高影响因子
ARCHIV DER PHARMAZIE
·
2022
·Vol. 356
·期 2
·2200191
·
IF 4.613
Pengqin Chen,Zhengwei Xu,Xiemin Wang,Jie He,Jun Yang,Jun Wang,Nipon Chattipakorn,Di Wu,Qidong Tang,Guang Liang,Ting Chen
The blockade of the overexpression of pro-inflammatory cytokines by anti-inflammatory natural products has been proven therapeutically beneficial in the treatment of acute lung injury (ALI). Given the fact that cinnamic acid has been proven to have significant anti-inflammatory activity, we selected it as a promising lead compound to develop more effective analogs in treating ALI. Learning from the symmetric structure of curcumin, 32 new symmetric cinnamic derivatives were designed, synthesized, and evaluated for their anti-inflammatory activity. Among them, 6h not only displayed a remarkable inhibitory activity in vitro (85.9% and 65.7% for IL-6 and TNF-α, respectively) without cytotoxicity but also possessed chemical structure stability. Furthermore, an in vivo study in mice revealed that the administration of 6h significantly attenuated lipopolysaccharide-induced ALI, providing new lead structures for the development of anti-inflammatory drugs for the treatment of ALI.
研究领域:
药理学
·
炎症研究
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
PLoS One
·
2016
·Vol. 11
·期 4
·e0154410
·
IF 3.057
Yanan Wu,Yanmin Zhou,Changhui Lu,Hussain Ahmad,Hao Zhang,Jintian He,Lili Zhang,Tian Wang
The study was conducted to evaluate the effects of dietary butyrate loaded clinoptilolite (CLI-B) on growth performance, pancreatic digestive enzymes, intestinal development and histomorphology, as well as antioxidant capacity of serum and intestinal mucosal in chickens. Two hundred forty 1-day-old commercial Arbor Acres broilers were randomly assigned to 4 groups: CON group (fed basal diets), SB group (fed basal diet with 0.05% sodium butyrate), CLI group (fed basal diet with 1% clinoptilolite), and CLI-B group (fed basal diet with 1% CLI-B). The results showed that supplementation of CLI-B significantly decreased (P < 0.05) feed conservation ratio at both 21 and 42 days of age, improved the pancreatic digestive enzymes activities (P < 0.05), increased the villus length and villus/crypt ratio (P < 0.05), and decreased the crypt depth of intestine (P < 0.05) as compared to the other experimental groups. Furthermore, the CLI-B environment improved the antioxidant capacity by increasing the antioxidant enzyme activities (P < 0.05) in intestine mucosal, and decreasing the NO content and i NOS activity (P < 0.05) in serum. In addition, CLI-B supplementation had improved the development of intestine and antioxidant capacity of broilers than supplementation with either clinoptilolite or butyrate sodium alone. In conclusion, 1% CLI-B supplementation improved the health status, intestine development and antioxidant capacity in broiler chickens, thus appearing as an important feed additive for the poultry industry.
研究领域:
动物营养学
·
家禽科学
·
兽医学
✓ 已验证
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2022
·Vol. 23
·期 3
·1-12
·
IF 4.026
Fu Xinzhen,Xu Shiji,Li Zhi,Chen Kun,Fan Huaying,Wang Yu,Xie Zeping,Kou Lijuan,Zhang Shumin
Cannabidiol (CBD) has poor water solubility and is subjected to extensive first-pass metabolism. These absorption obstacles are responsible for low and variable oral bioavailability of CBD. This study endeavored to improve CBD bioavailability by intramuscular (IM) injection of CBD nanocrystals (CBD-NC). The nanocrystals were prepared by antisolvent precipitation method and were characterized in terms of the particle size, polydispersity index (PDI), zeta potential, morphology, and crystalline status. CBD-NC displayed a particle size of 141.7±1.5 nm, a PDI of 0.18±0.01, and a zeta potential of −25.73 mV. CBD-NC freeze-dried powder using bovine serum albumin (BSA) as cryoprotectant had good redispersibility, and the average particle size was 139.1±1.4 nm after reconstitution. Moreover, these freeze-dried powders were characterized for drug loading and pH and were evaluated for in vitro dissolution and in vivo studies in a rat model. The in vivo results showed that AUC 0–24 h and C max of CBD by IM injection of CBD nanocrystals increased significantly compared with that of oral (P.O) administration of CBD nanocrystals and CBD oil solution. This underlines the nano-sized CBD could be suggested as a practical and simple nanosystem for IM delivery with improved bioavailability. More importantly, these results pave the way for future development of CBD-NC retentive dosage forms.
Graphical abstract
研究领域:
药剂学
·
药物递送系统
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Wen Liao,Can Xu,Xiang Wu,Qiuyan Liao,Ying Xiong,Zhen Li,Hongding Tang
Three new photobleachable cinnamoyl dyes for radical visible photoinitiators (CAs) were prepared by aldol condensation reaction of 2, 4-bis(diethylamino) benzaldehyde and esters (including ethyl acetate, diethyl malonate and ditertbutyl malonate), in comparison with CAs, the ditertbutyl 2-(4-(diphenylamino)benzylidene) malonate (TA) was also prepared. Their structures were confirmed by 1 H NMR, 13 C NMR, HR-MS. The mechanisms of the radicals generated from CAs and TA were proposed through electron paramagnetic resonance (EPR) analysis and steady state photolysis experiments. The investigated CAs and TA can effectively initiate the photopolymerization of 1, 6-hexanediol diacrylate (HDDA) under LED@405 nm exposure. Among these photoinitiators, CA-2 showed the best visible light photoinitiating efficiency and photobleachable characteristic. Furthermore, the residual initiators in cured HDDA polymers were proved to be as low as 0.243% and the obtained colorless polymer was up to 2.8 mm thickness when CA-2 was employed as the photoinitiator. These efficient photobleachable visible initiators show great potentials in deep curing materials.
研究领域:
材料科学
·
化学工程
·
高分子化学
✓ 已验证
DOI
高影响因子
ANALYTICAL LETTERS
·
2014
·
IF 1.019
Zhiguo Ma,Zhenya Yang,Danyi Lu,Long Dai,Baojian Wu,Shun Yao
Cistanche deserticola (Orobanchaceae) has been widely used in China for food and medicinal purposes. Chemical differentiation between the raw and steamed C. deserticola was performed by high-performance liquid chromatography coupled with diode array detection and mass spectrometry. Eight chemicals (echinacoside, cistanoside A, acteoside, cistanoside C, 2′-acetylacteoside, isoacteoside, isocistanoside C, and tubuloside B) were obtained from raw and steamed C. deserticola, and the kinetics in the steaming process were investigated in detail. When the steaming time increased, the concentrations of echinacoside, cistanoside A, acteoside, cistanoside C, and 2′-acetylacteoside decreased, while the levels of isoacteoside, isocistanoside C, and tubuloside B increased. Furthermore, two compounds, 5-hydroxymaltol and 5-hydroxymethylfurfural, were found only in the steamed form. In addition, hierarchical clustering analysis and principal component analysis were performed to evaluate the classify the chemical concentrations among steamed C. deserticola. The results showed that steaming had a significant influence on the chemical constitution. The study provide chemical analysis of raw and steamed C. deserticola, including possible transformation pathways, and should allow better understanding of the steaming process of this herb.
研究领域:
中药学
·
分析化学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Arun Saini,Chandravati Yadav,Sushanta K. Sethi,Bai-Liang Xue,Yuanyuan Xia,Ke Li,Gaurav Manik,Xinping Li
This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures. In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC). To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs. At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture. The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures. Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3–5 mg/mL), and Live/Dead BacLight viability tests. The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy. The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial. Furthermore, these architectures demonstrated excellent antibacterial performance under real “in use” contamination prone conditions. Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
研究领域:
材料科学
·
生物医学工程
·
天然产物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Zhiming Yan,Biqing Hu,Qianlian Li,Sunxian Zhang,Jie Pang,Chunhua Wu
In this work, covalent organic frameworks SNW-1 incorporated polyacrylonitrile (SNW-1@PAN) nanofiber was synthesized and used as pipette tip solid phase extraction (PT-SPE) adsorbent for preconcentration of five sulfonamides (SAs) in meat samples. The SNW-1@PAN nanofiber was fabricated via two steps, including the hydrothermal synthesis of SNW-1 and co-electrospinning of the SNW-1/PAN mixture. The three dimensional networks of electrospun nanofiber provide both low backpressure and rapid mass transfer in PT-SPE application, thus preventing the problems of high backpressure and leakage caused by directly packing SNW-1 into PT-SPE cartridge. The SNW-1@PAN nanofiber packed PT-SPE cartridge was used to extract SAs in meat samples followed by high performance liquid chromatography (HPLC). The effects of different parameters influencing the extraction and desorption efficiency were intensively investigated and optimized. Under the optimum conditions, the linear ranges of SAs were in the range of 5–125 ng mL −1 with correlation coefficients above 0.998, and the limits of detection of the five SAs ranged from 1.7 to 2.7 ng mL −1 . The interday and intraday precision (RSD) for five replicate extractions of the five SAs (50 ng mL −1 for each) was in the range of 5.4–9.2%. The applicability of the proposed method was examined by analyzing the SAs in the pork and chicken samples. The recoveries of the five SAs varied in the range of 86–111% with good precision, showing the good capability of the proposed method for the determination of SAs in the complicated real samples.
研究领域:
分析化学
·
材料科学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Yinghua Gao,Zhuoying Wang,Xinmeng Jin,Xiaoli Wang,Yining Tao,Shandeng Huang,Yun Wang,Yingqi Hua,Xuran Guo,Jing Xu,Zhengdong Cai
Osteosarcoma (OS) is a “cold” tumor enriched in noninflammatory M2 phenotype tumor-associated macrophages (TAMs), which limits the efficacy of immunotherapy. The acidic tumor microenvironment (TME), generated by factors such as excess hydrogen (H + ) ions and high lactate levels, activates immunosuppressive cells, further promoting a suppressive tumor immune microenvironment (TIME). Therefore, a multitarget synergistic combination strategy that neutralizes the acidic TME and reprograms TAMs can be beneficial for OS therapy. Here, a calcium carbonate (CaCO 3 )/polydopamine (PDA)-based nanosystem (A-NPs@(SHK+Ce6)) is developed. CaCO 3 nanoparticles are used to neutralize H + ions and alleviate the suppressive TIME, and the loaded SHK not only synergizes with photodynamic therapy (PDT) but also inhibits lactate production, further reversing the acidic TME and repolarizing TAMs to consequently lead to enhanced PDT-induced tumor suppression and comprehensive beneficial effects on antitumor immune responses. Importantly, A-NPs@(SHK+Ce6), in combination with programmed cell death protein 1 (PD-1) checkpoint blockade, shows a remarkable ability to eliminate distant tumors and promote long-term immune memory function to protect against rechallenged tumors. This work presents a novel multiple-component combination strategy that coregulates the acidic TME and TAM polarization to reprogram the TIME.
研究领域:
肿瘤学
·
免疫学
·
纳米医学
✓ 已验证
DOI
高影响因子
Science Advances
·
2021
·Vol. 7
·期 34
·
IF 14.143
Wei Tang,Yangqiao Lin,Chao Zhang,Yuwen Liang,Jinrong Wang,Wei Wang,Chen Ji,Maoying Zhou,Huayong Yang,Jun Zou
Soft robotics revolutionized human-robot interactions, yet there exist persistent challenges for developing high-performance soft actuators that are powerful, rapid, controllable, safe, and portable. Here, we introduce a class of self-contained soft electrofluidic actuators (SEFAs), which can directly convert electrical energy into the mechanical energy of the actuators through electrically responsive fluids that drive the outside elastomer deformation. The use of special dielectric liquid enhances fluid flow capabilities, improving the actuation performance of the SEFAs. SEFAs are easily manufactured by using widely available materials and common fabrication techniques, and display excellent comprehensive performances in portability, controllability, rapid response, versatility, safety, and actuation. An artificial muscle stretching a joint and a soft bionic ray swimming in a tank demonstrate their effective performance. Hence, SEFAs offer a platform for developing soft actuators with potential applications in wearable assistant devices and soft robots.
研究领域:
机器人学
·
材料科学
·
电气工程
·
生物力学
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2014
·
IF 2.819
Suxia Shen,Tiehua Zhang,Yuan Yuan,Songyi Lin,Jingyue Xu,Haiqing Ye
Gram-negative Escherichia coli (ATCC 8735) and Gram-positive Staphylococcus aureus (ATCC 3101) were selected as model bacteria to determine the antimicrobial mechanism of cinnamaldehyde . Several techniques were utilized to investigate the effects of cinnamaldehyde on food-borne bacterial membranes. The ultraviolet (UV) absorption and electrical conductivity of the culture supernatant were used to determine membrane integrity. β-Galactosidase activity was determined to detect inner membrane permeability. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were performed to observe bacterial morphology. Samples from both strains exposed to cinnamaldehyde showed higher UV absorptions, conductivity values, and β-Galactosidase activities compared with the control group and displayed a rapid rise trend. Thereafter, the values stabilized at a relatively steady state. SEM shows that treated E. coli and S. aureus cell samples exhibited rough cell membranes with particulate matter, and some of the S. aureus cells split due to deep wrinkle formation and distortion, unlike the control group. TEM shows that the bacteria treated with cinnamaldehyde exhibited numerous abnormalities, including cytoplasmic membrane separation from the cell wall, cell wall and cell membrane lysis, cytoplasmic content leakage, cytoplasmic content polarization, cell distortion, and cytoplasmic content condensation. These results indicate that bacterial cell morphology, membrane integrity, and permeability are damaged when the E. coli and S. aureus cells are exposed to the minimum inhibitory concentrations of cinnamaldehyde (0.31 mg/mL). In addition, the higher the cinnamaldehyde concentration, the more serious the bacterial membrane damage is.
研究领域:
微生物学
·
食品科学
·
分子生物学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Shengming Zhao,Yu Liu,Xiaorui Yuan,Yanyan Zhao,Zhuangli Kang,Mingming Zhu,Hanjun Ma
The effects of different intensities of low-frequency alternating magnetic fields (LF-AMF) modification on gel characteristics, rheology, water distribution, secondary structure and microstructure were investigated. The results showed that application of LF-AMF exhibited a significant improvement of the water-holding capacity, gel strength, color, textural properties and acceptability of low-salt pork batter gels ( P < 0.05). When the LF-AMF intensity reached 0.6 mT, the low-salt pork batters had the highest WHC, L* , gel strength, hardness, springiness, gumminess and chewiness and acceptability ( P < 0.05). During the temperature (sample heated from 20 to 80 °C) and frequency (0–10 Hz) sweep, the storage modulus G′ first increased and then decreased with increasing of LF-AMF intensity. Low-field nuclear magnetic resonance (LF-NMR) showed that LF-AMF at an intensity of 0.6 mT shortened the T 2 relaxation time of pork batter gel and increased the proportion of immobilized water. Fourier transform infrared spectroscopy (FTIR) showed that LF-AMF treatment significantly induced an increase of β-sheet structure and reduction of α-helix content in pork batter gels ( P < 0.05). Scanning electron microscopy (SEM) results suggested that LF-AMF contributed to the formation of pork batter gels with a more uniform and orderly network structure.
研究领域:
食品科学
·
生物物理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）