JOURNAL OF SEPARATION SCIENCE
·
2013
·
IF 2.591
Jing Chen,Juan Zhang,Wenpeng Zhang,Zilin Chen
Sarcosine, a potential biomarker of prostate cancer, has drawn great attention in recent years. However, controversial research keeps arising about its role as a biomarker that might come from the two isomers (α-alanine and β-alanine) of sarcosine due to their same molecular weight and similar properties, which could interfere with the accurate detection of sarcosine. In this study, a simple and sensitive method was developed for the detection of sarcosine and the two isomers by LC with ion-trap MS through a novel derivatization reagent N , N ′-dicyclohexylcarbodiimide. N , N ′-Dicyclohexylcarbodiimide is usually considered as a condensation reagent, however, it was directly used as a derivatization reagent through a rearrangement side reaction in this study. The proposed method not only improved the chromatographic retention behavior of sarcosine and its two isomers, which was a benefit to their separation, but also dramatically enhanced the detection sensitivity of sarcosine, which was more favorable for real sample analysis. The factors affecting the productivity of the derivatization reaction, such as reaction time and amount of derivatization reagent, were systematically optimized. The method shows good linearity ( R 2 > 0.99), sensitivity with LODs of sarcosine as low as 1 ng/mL, and repeatability with the RSD < 6.07%. The developed method was applied to the analysis of urine.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plasmonics
·
2015
·Vol. 11
·期 2
·587-591
·
IF 2.238
He Yi,Liang Yun,Song Hu
Creatinine-functionalized AuNPs (CreAuNPs) were prepared via a facile one-pot reaction of sodium borohydride and the mixture solution of gold(III) chloride trihydrate and creatinine. The morphology and surface state of as-prepared CreAuNPs were characterized by transmission electron microscopy and Fourier transform infrared spectroscopy. All the results demonstrated that CreAuNPs were spherical with an average diameter of about 4.2 nm, and creatinine existed on the surface of AuNPs via Au-N interaction. The as-prepared CreAuNPs exhibited a weak surface plasmon resonance (SPR) absorption owing to their small size, while the addition of Ag + could induce the aggregation of spherical CreAuNPs, producing a strong SPR absorption and apparent color change from colorless to purple owing to the surface plasmon coupling. On this basis, a colorimetric assay for Ag + was established. The assay could selectively detect Ag + as low as 1 μM with a good linearity in the range of 5–40 μM. Additionally, the assay was successfully applied to the determination of Ag + in tap water, lake water, and river water samples.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2749-2757
·
IF 4.58
Mayang Yanchao,He Xiwen,Chen Langxing,Zhang Yukui
A surface plasmon resonance sensor whose surface has been modified with boronic acid groups was developed in order to bind and detect transferrin. 4-Mercaptophenylboronic acid (4-MPBA) was utilized to form a self-assembled monolayer on the gold surface of the sensor chip. The surface topography of the modified gold chip was characterized by atomic force microscopy. In solutions of pH 9, the glycosyl groups of transferrin (Trf) quickly and reversibly combine with 4-MPBA on the sensor chip to result in changes of the refractive index. Thus, Trf can be sensed online by monitoring the SPR signal. Under optimized conditions, the sensor has a linear response in the 10 nM to 1.3 μM Trf concentration range, and the limit of detection is 4.4 nM (equivalent to 0.34 μg·mL −1 ). The results suggest that this 4-MPBA-functionalized sensor is a viable candidate for clinical testing of Trf which is a potential marker for severe diseases such as hypertension, primary renal disease, and diabetes.
Schematic of a novel SPR sensor modified with boronic acid for the determination of transferrin in human urine with high sensitivity.
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2024
·Vol. 15
·期 1
·9
·
IF 4.9
Changyun Quan,Yue Zhang,Yuanyuan Liu,Liping Wen,Haixia Yang,Xueqin Huang,Minghui Yang,Binjie Xu
Electrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode. Then, a commercial disposable electrochemical glucose sensor was constructed based on screen-printing electrodes. Experimental results indicated the synergy between PD and Ru(III) provided a promising electron transfer environment for a glucose dehydrogenase (GDH)-catalyzed glucose reaction. The sensor exhibits a linear glucose response range from 0.01 to 38.6 µmol/L, with a limit of detection (LOD) as low as 7.0 µmol/L and a sensitivity of 38 µA·L/(mmol·cm2). The accuracy of the sensor was further validated in spiked recovery tests of human venous blood samples. The glucose recovery rate was between 99.5% and 107%, with a relative standard deviation (RSD) of less than 3.2%. These results demonstrate that our sensor has high potential for commercialization and practical application in glucose monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Zhaoxian Chen,Shihong Liu,Wenrou Yu,Li Wang,Fengxue Lv,Liejun Yang,Huiqing Yu,Haiyang Shi,Yingzhou Huang
Comfortable treatment of malignant tumors is the clinical orientation of cancer therapy at present, which puts forward a high demand for non-invasive, portable and high-frequency monitoring status of tumor in the treatment. Unlike traditional blood and X-ray technique, here we have developed hydrogel-based wearable sweat sensors, equipped with multiple molecular receptors for surface enhanced Raman spectroscopy (SERS) monitoring treatment effects of lung cancer. The SERS technique was utilized in combination with multiple artificial intelligence (AI) algorithms (LGB GNB, LDA, RF, and SVM) to develop a novel and precise diagnostic model for monitoring the treatment effect. Based on 12617 SERS spectras from clinical patients, the results successfully diagnosed three treatment effects (progressive disease, partial response, and no change) with an accuracy of 89.7 %. Benefiting from data mining in AI algorithms, key Raman spectra features in clinical spectra are identified to explore characteristic biomarkers of lung cancer associated with various comorbidities. The clinical data suggest that carbonyl biomarkers in sweat might be crucial for understanding complications such as diabetes and hypertension. Our results not only offer a novel and comfortable monitoring technique but also enable personalized treatment of lung cancer with complications, presenting significant potential for clinical application.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Chiyu Ma,Yong Qiu,Tao Liang,Nan Jiang,Liubing Kong,Jianguo Wu,Xianyou Sun,Guangqing Ren,Xinwei Wei,Ping Wang,Hao Wan
Background Ionic calcium (Ca 2+ ) plays a crucial role in maintaining normal physiological and biochemical functions within the human body. Detecting the concentration of Ca 2+ is of utmost significance for various purposes, including disease screening, cellular metabolism research, and evaluating drug effectiveness . However, current detection approaches such as fluorescence and colorimetry face limitations due to complex labeling techniques and the inability to track changes in Ca 2+ concentration. In recent years, extensive research has been conducted in this field to explore label-free and efficient approaches. Results In this study, a novel light-addressed potentiometric sensor (LAPS) using silicon-on-sapphire technology , has been successfully developed for Ca 2+ sensing. The Ca 2+ -sensitive LAPS achieved a wide-range detection of Ca 2+ , ranging from 10 −2 M to 10 −7 M, with an impressive detection limit of 100 nM. These advancements are attributed to the ultra-thin silicon layer, silicon dioxide layer, and solid-state silicon rubber sensitive membrane around 6 μm. Furthermore, the sensor demonstrated the ability to dynamically monitor fluctuations in Ca 2+ concentration ranging from 10 −9 M to 10 −2 M within a solution. Its remarkable selectivity , specificity, and long-term stability have facilitated its successful application in the detection of Ca 2+ in human serum and urine. Significance and novelty This work presents a Ca 2+ -sensitive sensor that combines a low detection limit and a wide detection range. The development represents the emergence of a label-free and rapid Ca 2+ detection tool with immense prospects in home-based health monitoring, community disease screening, as well as cellular metabolism, and drug screening evaluations.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Hongyu Yin,Zhoujun Wang,Xiang Zhang,Weifeng Zhao,Ran Wei,Changsheng Zhao
Chronic liver failure (CLF) and chronic renal failure (CRF) lead to toxins accumulation, severely impairing organ functions. To address this challenge, a Janus polyethersulfone (PES)-based architecture integrated dense membrane with porous monolith is developed, pioneering an approach for simultaneous plasma separation and toxins adsorption. The porous monolith, featuring high porosity about 83.3 % and substantial specific surface area about 102.06 m 2 /g, is prepared using the freezing-induced phase separation (FIPS) technique. The porous monolith is composed of polymer networks of PES and amphiphilic copolymer of poly (vinyl pyrrolidone-co-methyl methacrylate) (VM), as well as activated carbon. Among the various monolith, the PES 18 VM 5 C 2 exhibits the optimal balance of adsorption and anti-protein adhesion. The PES-VM-incorporated dense membrane with a controlled submicron pore size is constructed atop the PES 18 VM 5 C 2 . The dense membrane effectively blocks hemocytes and the porous monolith efficiently adsorbs toxins. Together, the Janus architecture PES 18 VM 5 C 2 @M 16 allows over 85 % of bovine serum albumin (BSA) to permeate, showcasing its selective permeability. After being integrated into a custom 3D-printed supporting device, the PES 18 VM 5 C 2 @M 16 achieves significant clearance in creatinine (67.6 %), uric acid (87.4 %), and bilirubin (89.1 %) during 2-hour plasma adsorption test, with minimal impact on essential plasma components such as total protein, albumin, and cholesterol. This work presents a “Janus Interface Architecture” as next-generation platform for plasma separation and toxins adsorption, offering a promising strategy for wearable artificial liver systems.
✓ 已验证
产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2024
·Vol. 87
·期 6
·407-418
·
IF 1.2
Hong Lei,Mao Huihui,Huang Xiaowei,Fu Xinyang,Lin Yanfang
To analyze 1-methyladenosine (m1A) and 1-methylinosine (m1I) in urine samples obtained from patients with breast cancer and healthy people, we used magnetic dispersive solid-phase micro-extraction (MDSPME) combined with high-performance liquid chromatography. The structure and morphology of magnetic nanoparticles, Fe 3 O 4 @GO, were investigated by FT-IR, XRD, TEM, and TGA. m1A and m1I in urine samples were enriched by the solid phase micro-extraction technology of Fe 3 O 4 @GO. Several parameters affecting the extraction efficiency, including adsorption time, pH, ionic strength, mass of adsorbent, extraction time, eluent solvent, elution time, and elution temperature, were researched and optimized. Under the optimized conditions, the corresponding linearity range of the two analytes showed a good linearity ( r 2 > 0.9998) and significant addition recoveries ranging from 88.50 to 109.38%, while the limit of detection was between 0.10 and 0.11 ng/mL. The enrichment factors of m1A and m1I were 108 and 134, respectively. The method was simple, sensitive, and reliable for the detection and purification with good detection limit, and it was suitable for the detection of m1A and m1I in urine samples. In addition, the analysis of modified nucleosides is of great value for the early diagnosis of patients with breast cancer.
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Mantong Zhao,Zhongyuan Liu,Mengyin Zha,Ying Sun,Haohao Shi,Xueying Zhang,Chuan Li,Guanghua Xia
The effect of aldehydes from lipid oxidation on the generation of heterocyclic amine 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) during pan-frying of golden pompano fillets was investigated. It was observed the aldehydes that contribute to thiobarbituric acid reactive substances (TBARS) value significantly promoted PhIP generation ( p < 0.05). The defat treatment of fresh fish tissue significantly reduced the PhIP formation in pan-fried products ( p < 0.05). During pan-frying, massive aldehydes were generated in a time-dependent manner with acrolein, propanal, hexanal, 4-hydroxy-nonenal (HNE), and 2,4-decadienal (DDE) as the abundant species. In the established chemical model, these aldehydes congruously promoted PhIP formation with the increased concentration, especially acrolein, HNE and DDE. Therefore, aldehydes could significantly enhance the PhIP generation under processing. However, except for propanal and hexanal, the promoting effect was slightly decreased with high levels of aldehydes addition due to the strong electrophilic properties and participation of reaction with the amino group of phenylacetaldehyde, creatinine, even PhIP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Yingfei Hui,Hao Guo,Mingyue Wang,Liping Peng,Borong Ren,Yanyan Ma,Wu Yang
Glutathione (GSH) levels have been linked to aging and the pathogenesis of various diseases, highlighting the necessity for the development of sensitive analytical methods for GSH to facilitate disease diagnosis and treatment. In this study, we synthesized a novel core-shell material, UiO@TBTA, by in-situ growing TFPB-TAPA COF on UiO-66-NH 2 through a Schiff base reaction. The resulting composite capitalize on the advantages of both materials, demonstrating excellent stability, large specific surface area, and abundant active functional groups while preserving superior crystallinity. Notably, this strategy effectively reduces the occurrence of aggregation-caused quenching (ACQ) in COFs. Due to the inner filter effect and hydrogen bonding interactions between UiO@TBTA and GSH, a specific ratiometric fluorescence detection of GSH was achieved in the range of 0.1–7 μM, with a limit of detection (LOD) of 0.0685 μM. In addition, due to the sensitive color change of the sensing material from orange to black caused by GSH, a proportional colorimetric sensing strategy has also been proposed, enabling the detection of GSH within the range of 1–200 μM. What's more, two intelligent artificial neural networks models were constructed with the help of machine learning that can quickly, accurately, and sensitively determine the concentration of GSH based on fluorescence images and color photographs respectively. Our work represents the first study utilizing MOF@COF composite for the multimodal detection of GSH, thus providing a novel strategy for the multimodal detection of the target analyte. Prospectively, the construction of the fluorescence/colorimetric dual-mode intelligent ratiometric sensing platform using deep machine learning holds great promise for real-time monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Longwei Jiang,Iftikhar Hussain Badar,Ziwen Yang,Guanhua Li,Yingying Hu,Lang Zhang
This study examined how varying roasting times influenced the formation of free and bound heterocyclic aromatic amines (HAAs) in beef during the roasting process. The findings showed that as the roasting time increased, the water content and color decreased ( P < 0.05), whereas glucose, creatine, and creatinine levels increased ( P < 0.05) from 0.05 to 2.45, 2.68–4.96, and 0.07–0.91 mg/g, respectively. A total of 7 free HAAs (IQx, MeIQx, MeIQ, PhIP, 4,8-DiMeIQx, Harman, and Norharman) and 5 bound HAAs (MeIQx, AαC, 7,8-DiMeIQx, Harman, and Norharman) were detected in the roasted beef by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The bound HAA content in the roasted beef was significantly higher than that of the free HAAs. As the roasting time increased, the bound HAAs increased from 2125.08 to 2326.45 ng/g, while the free HAAs increased from 45.72 to 121.38 ng/g ( P < 0.05). Correlation analysis revealed that the development of free and bound HAAs was positively associated with glucose, creatine, and creatinine, and the FAAs were positively correlated with the formation of MeIQx through partial least squares regression (PLSR) analysis. These findings would help minimize the development of free and bound HAAs in roasted meat products.
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Tian-Pei Zhao,Shu-Kun Wang,Hang Yu,Jing-Yi Gao,Ao-Han Zhang,Chen-Xia Zhang,Yu-Xiang Ma,Hua-Min Liu,Xue-De Wang
Flos Sophorae Immaturus extract (FSIE) is rich in flavonoids and phenolics. The effects of various concentrations (0.2, 0.4, and 0.6 g/100 g of meat) of FSIE on the quality and heterocyclic amine (HCA) formation of chicken patties fried at 170 °C and 190 °C were investigated for the first time. The total HCA content of chicken patties fried at 190 °C (7.23–13.73 ng/g) was remarkably higher than that of patties fried at 170 °C (2.34–2.57 ng/g). It is noteworthy that most HCAs formed on the surface of the patties. FSIE at 0.2–0.6 g/100 g significantly reduced the formation of HCAs in chicken patties fried at 190 °C by 27.09%–47.34%, which may be related to the enhanced total phenolics, total flavonoids, and antioxidant activities of FSIE after heating. With the addition of FSIE, the L ∗ and a ∗ values of meat patties decreased and b ∗ values increased. However, the addition of FSIE to chicken did not negatively affect the sensory attributes of fried chicken patties. Also, FSIE somewhat reduced the TBARS and carbonyl values of fried chicken patties. Correlation analysis revealed that the protein carbonyl value of meat patties was significantly and positively correlated with the total HCA formation (r = 0.90, p < 0.01). These results provide evidence that the addition of FSIE to high-temperature fried products, especially on the product surface or in the frying medium, can effectively reduce the formation of hazardous HCAs.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）