SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Mengzhen Jiang,Ruohan Xu,Chunyan Yang,Haili Wang,Jinyuan Cai,Yanrong Wen,Mingfu Ye,Tingxuan Yan,Shuangshou Wang
cis -Diol compounds are of great biological and clinical values, but their detection is usually instrument-inseparable. Herein, a flexible sensing strategy was proposed at the first time for multi-mode visualization of cis -diol compounds based on boronate affinity (BA)-mediated colorimetry and fluorometry. Boronic acid-decorated sensors with color-/fluorescence-derived binary responsivity were designed using cis -diol fluorescent dyes or dyes-fluorophore counterparts as response units, inducing competitive BA when cis -diol analytes were existed. Consequently, colors and fluorescence of sensors changed simultaneously, and both signals were linearly varied versus the dosages of cis -diol analytes in certain ranges. As proofs of concept, dual-mode visual sensing of glucose in normal/hyperglycemic human serum and sucrose in canned white fungus and edible mushroom plant beverage was achieved with sensing time as short as 1 min and limit of detection as low as 0.18 mg/mL. Furtherly, color information of colorimetric/fluorometric cards and tested sensors was picked by a smartphone APP for digital image analysis, by which the validity of dual-mode visual sensing assays was reinforced by avoiding possible errors from subjective judgement on colors and fluorescence. Besides, the credibility of visual sensing was cross-validated by fluorescent quantification and classical tools such as portable blood glucose meter and phenol-sulfuric acid method-based halochromic reaction.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2012
·Vol. 404
·期 5
·1477-1484
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IF 3.778
Zhang Lei,Dai Xiaojun,Xu Fei,Wang Fuqiang,Gong Bolin,Wei Yinmao
A novel imidazole-functionalized stationary phase for hydrophilic interaction chromatography (HILIC) was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP). 1-Vinylimidazole as a monomer was polymerized on the surface of initiator-immobilized silica by SI-ATRP using CuCl and 2,2′-bipyridyl as a catalyst. The graft chain length and polymer grafting density were controlled by varying the ratio of monomer to initiator. The resulting materials were characterized by elemental analysis and thermogravimetric analysis. Then, high-performance liquid chromatography separation of eight nucleobases/nucleosides was performed on the imidazole-functionalized chromatographic column in HILIC mode. The effects of mobile phase composition, buffer pH, and column temperature on the separation of nucleobases/nucleosides were investigated, and the retention mechanisms were studied. Chromatographic parameters were calculated, and the results showed that surface adsorption through hydrogen bonding and electrostatic interaction dominated the retention behavior of the solutes in HILIC mode. Lastly, the stationary phase was successfully used to determine the nucleobases and nucleosides from Cordyceps militaris .
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JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 39
·期 12
·2365-2373
·
IF 2.741
Li Gao,Yinmao Wei
Various cotton fiber based boronate-affinity adsorbents are recently developed for the sample pretreatment of cis -diol-containing biomolecules, but most do not have efficient capacity due to limited binding sites on the surface of cotton fibers. To increase the density of boronate groups on the surface of cotton fiber, polyhedral oligomeric silsesquioxanes were used to modify cotton fiber to provide plentiful reactive sites for subsequent functionalization with 4-formylphenylboronic acid. The new adsorbent showed special recognition ability towards cis -diols and high adsorption capacity (175 μg/g for catechol, 250 μg/g for dopamine, 400 μg/g for adenosine). The in-pipette-tip solid-phase extraction was investigated under different conditions, including pH and ionic strength of solution, adsorbent amount, pipette times, washing solvent, and elution solvent. The in-pipette-tip solid-phase extraction coupled with high-performance liquid chromatography was used to analyze four nucleosides in urine samples. Under the optimal extraction conditions, the detection limits were determined to be between 5.1 and 6.1 ng/mL (S/N = 3), and the linearity ranged from 20 to 500 ng/mL for these analytes. The accuracy of the analytical method was examined by studying the relative recoveries of analytes in real urine samples with recoveries varying from 83 to 104% (RSD = 3.9–10.2%, n = 3).
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BIOMEDICAL CHROMATOGRAPHY
·
2016
·Vol. 31
·期 4
·e3860
·
IF 1.729
Yan Du,Yin-jie Li,Xun-xiu Hu,Xu Deng,Zeng-ting Qian,Zheng Li,Meng-zhe Guo,Dao-quan Tang
As essential endogenous compounds, nucleobases and nucleosides fulfill various functions in living organisms. This study presents the development and validation of a new hydrophilic interaction liquid chromatography tandem mass spectrometry method for simultaneous quantification of 19 nucleobases and nucleosides in rat plasma. For the sample preparation, 15 kinds of protein precipitants were evaluated according to the chromatographic profile and ion response of analytes. The optimization of chromatographic separation was respectively performed using reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography mode; each separation mode included two test columns with different stationary phases. The chromatographic profile and parameters such as half-width ( W 1/2 ), capacity factor ( K ′) and tailing factor ( f t ) were used to evaluate the separation efficiencies. Furthermore, the adopted composition of two mobile phase systems and the concentrations of the additives in the optimum buffer system were also investigated. The developed method was fully validated and successfully applied quantitatively to determine 19 nucleobases and nucleosides in plasma from normal and diabetic nephropathy (DN) rats. Significant differences between normal and DN rats were found in plasma levels of cytosine, xanthine, thymidine, adenosine, guanosine, inosine and 8-hydroxy-2′-deoxyguanosine. This information may provide a useful reference for the discovery of potential biomarkers of DN.
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NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 17
·9230-9235
·
IF 3.269
Xin Shen,Lei Xu,Wanying Zhu,Bingzhi Li,Junli Hong,Xuemin Zhou
In this paper, a novel turn-on fluorescence aptasensor has been designed for adenosine detection based on fluorescence resonance energy transfer (FRET) from single-stranded DNA labeled carbon dots (ssDNA-CDs) to aptamer modified gold nanoparticles (aptamer-AuNPs). In the absence of adenosine, the fluorescence of ssDNA-CDs was quenched by aptamer-AuNPs via the formation of an aptamer–ssDNA duplex. The introduced adenosine competed to displace ssDNA-CDs by specifically binding to the aptamer, resulting in the recovery of the quenched fluorescence of ssDNA-CDs. Under optimized conditions, the increase in fluorescence intensity was proportional to the concentration of adenosine with a linear range of 10–500 nM and allowed a limit of detection as low as 4.2 nM. In addition, this method was successfully applied to adenosine determination in human serum samples with satisfactory results.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2017
·
IF 3.255
Yang Huang,Tingting Zhang,Yumei Zhao,Haibo Zhou,Guangyun Tang,Marianne Fillet,Jacques Crommen,Zhengjin Jiang
Nucleobases, nucleosides and ginsenosides, which have a significant impact on the physiological activity of organisms, are reported to be the active components of ginseng, while they are less present in ginseng extracts. Few analytical methods have been developed so far to simultaneously analyze these three classes of compounds with different polarities present in ginseng extracts. In the present study, a simple and efficient analytical method was successfully developed for the simultaneous separation of 17 nucleobases, nucleosides and ginsenosides in ginseng extracts using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The effect of various experimental factors on the separation performance, such as the column type, temperature and backpressure, the type of modifier and additive, and the concentration of make-up solvent were systematically investigated. Under the selected conditions, the developed method was successfully applied to the quality evaluation of 14 batches of ginseng extracts from different origins. The results obtained for the different batches indicate that this method could be employed for the quality assessment of ginseng extracts.
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JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Jialiang Guo,Yuan Xiao,Yuanjing Lin,Jacques Crommen,Zhengjin Jiang
Low column efficiency for enantioseparations in capillary liquid chromatography (CLC) is a major problem commonly encountered with β -cyclodextrin ( β -CD) functionalized polymer-based monoliths. In order to investigate the effect of the crosslinker type on enantioseparation performance, three commonly used crosslinkers, i.e. 1,4-bis(acryloyl)piperazine (PDA), ethylene dimethacrylate (EDMA) and N , N -methylenebisacrylamide (MBA), were copolymerized using the one-pot approach with glycidyl methacrylate-mono-6-amino-6-deoxy- β -CD (GMA-NH 2 - β -CD) as functional monomer. The three monolithic columns, including poly(GMA-NH 2 - β -CD- co -PDA) and poly(GMA-NH 2 - β -CD- co -MBA), as well as the previously reported column poly(GMA-NH 2 - β -CD- co -EDMA) were systematically compared with respect to morphology, permeability, β -CD density, retention mechanism and efficiency. The enantioseparation ability of each column was evaluated using 14 chiral compounds, including mandelic acid derivatives, profens, N -derivatized amino acids, and herbicides, as test substances. The β -CD-functionalized monolith with MBA as crosslinker was found to exhibit higher polarity, higher column efficiency and better enantioseparation performance than those with PDA or EDMA as crosslinker.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2629-2636
·
IF 4.58
Cai Tianpei,Zhang Haijuan,Rahman A. F. M. Mustafizur,Shi Yan-Ping,Qiu Hongdeng
Carbon dots derivatized from N-(β-aminoethyl)-γ-aminopropyl-methyldimethoxysilane (AEAPMS) were coated onto silica microparticles. These particles (Sil-CDs) are shown to be an excellent stationary phase for use in hydrophilic interaction chromatography. Analytes including sulfonamides, nucleosides and bases, flavones and amino acids can be well separated on this stationary phase. Compared to a silica stationary phase functionalized with AEAPMS only, the Sil-CDs show enhanced separation performance. The selectivity factors of three nucleosides and bases (1.02–1.09) and four sulfonamides (1.04–1.11) on AEAPMS functionalized silica stationary phase were improved to 1.10–1.20 and 1.13–1.15 respectively on Sil-CDs stationary phase. This is attributed to the higher number of surface functional groups due to the introduction of carbon dots. The successful application of the Sil-CDs stationary phase highlights the potential of carbon dots as a modified material in chromatography.
Schematic presentation of the preparation of silanized carbon dots coated onto silica microparticles. The material represents a new stationary phase for hydrophilic interaction chromatography. It shows improved separation performance compared to a silane-only functionalized silica stationary phase.
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RSC Advances
·
2016
·Vol. 6
·期 34
·28470-28476
·
IF 3.289
Li Gao,Chaozhan Wang,Yinmao Wei
In this work, polyethyleneimine-modified boronate affinity fibrous cotton with good selectivity and higher binding capacity for cis-diols was developed for in-pipette-tip solid-phase extraction (SPE). The introduction of polyethyleneimine for the modification of cotton fiber provided abundant binding sites for adsorption of cis-diols, resulting in higher binding capacities up to 300 μg g−1 for catechol, 450 μg g−1 for dopamine and 700 μg g−1 for adenosine, respectively. The adsorbent could still show recognition ability towards cis-diols even with a 1000-fold amount of interferences in the solution. Several extraction parameters were optimized, including adsorbent dose, pipette times, washing and elution solvents. Under optimal extraction conditions, the proposed in-pipette-tip SPE method coupled with reversed-phase liquid chromatography was successfully used to extract nucleosides in urine. The limit of detection was 3.5–4.7 ng mL−1 and the recoveries were in the range of 89–102% with relative standard deviations of less than 9.9%. In conclusion, combination of the specific adsorption performance of the prepared boronate affinity fibrous material and in-pipette-tip SPE provided a fast pretreatment method of trace cis-diols in complex real samples.
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ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Li Cui,Ying-Jiao Zhang,Wei E. Huang,Bi-Feng Zhang,Francis L. Martin,Jun-Yi Li,Kai-Song Zhang,Yong-Guan Zhu
Bacterial antibiotic resistance poses a threat to global public health. Restricted usage of antibiotics does not necessarily prevent its continued emergence. Rapid and sensitive screening of triggers, in addition to antibiotic, and exploring the underlying mechanism are still major challenges. Herein, by developing a homogeneous vacuum filtration-based bacterial sample fabrication enabling high surface-enhanced Raman scattering (SERS) reproducibility across multiple bacterial samples and negating interfering spectral variations from inhomogeneous sample geometry and SERS enhancement, SERS was employed to study heavy metal arsenic [As(V)]-mediated antibiotic resistance in a robust, sensitive, and rapid fashion. Independent and robust spectral changes representing phenotypic bacterial responses, combined with multivariate analysis, clearly identified that As(V) enhanced antibiotic resistance to tetracycline (Tet). Similar spectral alteration profile to As(V) and Tet indicated that cross-resistance, whereby As(V)-induced bacterial resistance simultaneously blocked Tet action, could account for the enhanced resistance. The sensitive, robust, and rich phenotypic profile provided by SERS, combined with additional advantages in imposing no need to cultivate bacteria and single-cell sensitivity, can be further exploited to evaluate resistance-intervening factors in real microbiota.
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TALANTA
·
2016
·
IF 4.035
Huihui Li,Ting Cheng,Shuxia Wang,Xinyue Zhu,Haixia Zhang
Boronate affinity materials have been widely used for selective capture of cis-diol-containing molecules, but most do not have sufficient extraction efficiency. We have prepared boronic acid-functionalized attapulgite, and then it was coated with imidazolium-based ionic liquid , 1-dodecyl-3-methylimidazolium bromide (C 12 mimBr), via physical self-assembly process. The extraction efficiency of the material increased dramatically after coated with C 12 mimBr, and its enrichment ratios for nucleosides increased by 9- to 282-fold. Besides, the C 12 mimBr-coated adsorbent did not lose selectivity and was able to capture cis-diols in the presence of a 100-fold excess of interferences. C 12 mimBr-coated material was applied to selective enrichment of nucleosides from human urine. The limits of detection and the limits of quantification were in the range of 0.06–0.46 ng mL −1 and 0.20–1.53 ng mL −1 , respectively. Reproducibility of the method was determined with relative standard deviations≤9.9%. The recoveries of the target nucleosides from spiked human urine were in the range of 87.8–109.6%. In our preception, self-assembly ionic liquid layer can serve as a promising alternative to improve the extraction efficiency of boronate affinity materials.
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JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 39
·期 7
·1347-1356
·
IF 2.741
Ting Cheng,Shuqiang Zhu,Bin Zhu,Xiaoyan Liu,Haixia Zhang
The nucleoside or modified nucleoside level in biological fluids reflects the pathological or physiological state of the body. Boronate affinity absorbents are widely used to selectively extract nucleosides from complex samples. In this work, a novel functionalized absorbent was synthesized by attaching 4-mercaptophenylboronic acid to gold nanoparticles on modified attapulgite. The surface of the attapulgite was modified by poly(acryloyloxyethyltrimethyl ammonium chloride) by atom transfer radical polymerization, creating many polymer brushes on the surface. The resultant material exhibited superior binding capacity (30.83 mg/g) for adenosine and was able to capture cis -diol nucleosides from 1000-fold interferences. Finally, to demonstrate its potential for biomolecule extraction, this boronate affinity material was used to preconcentrate nucleosides from human urine and plasma.
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