FOOD CONTROL
·
2021
·
IF 5.548
Hui-Wen Gu,Xiao-Li Yin,Tian-Qin Peng,Yuan Pan,Hui-Na Cui,Zhi-Quan Li,Weiqing Sun,Baomiao Ding,Xian-Chun Hu,Zi-Hong Zhang,Zhi Liu
Identifying the geographical origins of green teas produced in specific regions is of significance since the geographical origin of tea influences its quality and price greatly. In this work, a novel two-dimensional (2D) fingerprints acquired by high-performance liquid chromatography-diode array detector (HPLC-DAD) was firstly proposed to identify geographical origins of Chinese green teas. A total number of 62 chemical components were extracted from 2D HPLC-DAD fingerprints of 78 tea samples by multivariate curve resolution-alternating least squares (MCR-ALS) algorithm. Afterward, principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to classify tea samples based on the extracted components. Inspection of PCA score plots of two scaling types (UV and Par) showed that tea samples from two different geographical origins have an obvious clustering tendency. As for the OPLS-DA analysis, the Q 2 cum of two types-scaling OPLS-DA models are greater than 0.75, and the total recognition rates for test set are 92.86%. What's more, according to p value of t -test, VIP values, V-plot and S-plot, 17 characteristic components were screened and identified as chemical markers to distinguish between Zhejiang teas and Shandong teas. This work indicated that the proposed strategy is suitable for identifying geographical origins of Chinese green teas.
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高影响因子
MOLECULES
·
2021
·Vol. 27
·期 1
·113
·
IF 4.412
Henghui Zhang,Xiaoli Wang,Dongliang He,Dongliang Zou,Runzhu Zhao,Huifang Wang,Shuying Li,Yongping Xu,Buhailiqiemu Abudureheman
In the present work, the extraction process of total flavonoids (TFs) fromX. sorbifoliaflowers by ultrasound-assisted extraction was optimized under the response surface methodology (RSM) on the basis of single-factor experiments. The optimal extraction conditions were as follows: ethanol concentration of 80%, solid–liquid ratio of 1:37 (g/mL), temperature of 84 °C, and extraction time of 1 h. Under the optimized conditions, the extraction yield of the TFs was 3.956 ± 0.04%. The radical scavenging capacities of TFs against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were much greater than that of rutin. The results of antibacterial experiments indicated that the TFs displayed strong inhibitory activities onE. coli,S. aureusandBacillus subtilis. Therefore,X. sorbifoliaflowers can be used as a novel source of natural flavonoids, and the TFs have potential applications as natural antioxidants or antibacterial agents in the food and pharmaceutical industries.Keywords:Xanthoceras sorbifoliaBunge;total flavonoids;ultrasound-assisted extraction;response surface methodology;antioxidant activity;antibacterial activity
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PubMed
高影响因子
Food and Bioprocess Technology
·
2023
·
IF 5.6
Li Jiangfei,Zhou Yajun,Lu Weihong
Vacuum freeze drying (VFD) has been widely used for drying berries, by combining with pretreatment technology, energy consumption and cost can be effectively reduced as well as product quality improved. However, the effect of cold plasma pretreatment (CPP) on the drying efficiency and quality characteristics of VFD haskap has not been investigated. In this study, an innovative drying technology, cold plasma pretreatment–assisted vacuum freeze drying, was applied to process haskap. The purpose of the present study was to explore the effect of different times of CPP (0–75 s) on VFD haskap performance. The results indicated that the application of CPP enhanced the effective diffusion rate and drying rate (27.68%) owing to pretreatment etching larger and more cracks in the microstructure, increasing cell membrane permeability (15.59%), and altering cell wall pectin composition. Midilli is the most recommended model for drying kinetics. CPP contributed to increased hydrophilicity (25.33%) and degraded enzymatic activity, thereby prompting the enhancement of polyphenols, anthocyanins, flavonoids, and antioxidant capacity. Besides, the physico-chemical properties were ameliorated, the possible mechanism for the enhanced drying rate and quality properties of VFD haskap by CPP was explained by chemometrics. Overall, CPP is a promising pretreatment technology for haskap VFD.
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高影响因子
Antioxidants
·
2023
·Vol. 12
·期 8
·1505
·
IF 7
Xuechun Zhang,Shi Li,Zhibin Zhang,Kin Weng Kong,Zhenxing Wang,Xiahong He
To assess the effects of microbial fermentation onGynostemma pentaphyllumleaves (GPL), four probiotics were used to ferment GPL (FGPL) for 7 days. At different stages of fermentation, changes in the active components and biological activities of FGPL were determined. The findings suggest that short-term fermentation with probiotics can enhance both the content and bioactivity of active components in GPL. However, prolonged fermentation may lead to a decline in these aspects. Among them, the best effect was observed with SWFU D16 fermentation for 2 days. This significantly improved the total phenolic and total flavonoid content, antioxidant capacity, and inhibitory ability against α-glucosidase activity with an increase of 28%, 114.82%, 7.42%, and 31.8%, respectively. The high-performance liquid chromatography (HPLC) analysis results also supported this trend. Untargeted metabolomics analysis revealed metabolite changes between GPL and FGPL and the key metabolites associated with these functional activities. These key metabolites are mainly organic acids, flavonoids, carbohydrates, terpenoids, and other substances. KEGG analysis demonstrated that microbial metabolism in diverse environments and carbon metabolism were the most significantly enriched pathways. Among them, 3-(3-hydroxyphenyl) propanoic acid, d-glucose, gallic acid, gluconic acid, l-lactic acid, and l-malic acid were mostly involved in the microbial metabolism of diverse environmental pathways. In contrast, D-glucose, gluconic acid, and l-malic acid were mainly related to the carbon metabolism pathway. This study revealed the positive effect of probiotic fermentation on GPL and its potential metabolism mechanism, which could provide supporting data for further research.Keywords:Gynostemma pentaphyllumleaves;probiotic fermentation;antioxidant capacity;α-glucosidase inhibitory activity;metabolomics
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PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Li Yang,Fengxiang Zhang,Weiwei He,Boyuan Zhao,Ting Zhang,Shang Wang,Lifen Zhou,Junwei He
Ethnopharmacological relevance Hosta plantaginea (Lam.) Aschers flowers (HPF) are well-known for their high flavonoid content, which contribute to their widely as traditional Chinese medicine for alleviating inflammation. Despite their recognized potential, information regarding the total flavonoid (TF) of HPF and its therapeutic application in treating chronic prostatitis (CP) remains unknown. Aim of the study We aimed to investigate the extraction optimization, constituent analysis, and alleviating effect of TF on CP as well as its potential mechanism. Materials and methods The optimized extraction of TF from HPF was explored using response surface methodology with a Box-Behnken design model. The major flavonoids in TF were identified based on UHPLC-MS approach. Efficacy of TF (25 and 100 mg/kg, p.o.) on CP was evaluated in prostate antigen emulsion-induced autoimmune CP rat model by measuring prostatic index, the levels of leukocytes and lecithin bodies, as well as histopathological examination. The protein expression contents were detected by western blotting . Additionally, the antioxidant (DPPH and ABTS) and anti-inflammatory (cyclooxygenase 2, COX-2 inhibitory) effects of TF were also evaluated in vitro . Results The optimized conditions for TF extraction were determined as 60% ethanol concentration, 30 mL/g liquid-to-solid ratio, 30 min extraction time, and 90 °C extraction temperature, and the extraction ratio is 65.98 ± 2.14%. A total of 15 major flavonoids in TF were characterized by comparison with reference standards. TF ameliorated the efficacy of CP in rats in a dose-independent manner, including reduced prostatic index and leukocytes levels, elevated lecithin body levels, ameliorated histopathological damage to prostate, and suppressed phosphorylated protein expressions of nuclear factor kappa-B (NF-κB) p65, inhibitor of NF-κB alpha (IκBα), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (Erk), just another kinase 1 (JAK1), signal transducer and activator of transcription 3 (STAT3), phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). Simultaneously, the IC 50 of TF to DPPH, ABTS radicals, and COX-2 were 2.02, 1.79, and 0.0838 mg/mL, respectively. Conclusions We first demonstrated that TF from HPF represents a promising candidate to alleviate CP through suppression of NF-κB, MAPKs, JAK-STAT, and PI3K-Akt signaling pathways.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yaqi Yang,Jiejun Li,Zhiwang Luo,Xingnian Li,Jiayi Zou,Chunyan Li,Chao Chen,Yixi Xie,Pengcheng Zhao,Junjie Fei
One-dimensional (1D) ordered mesoporous carbon hybrid nanotubes loaded with bimetallic oxide hollow nanospheres are expected to be very attractive electrocatalysts . In this study, through high-temperature pyrolysis , we generated Mo 2 C@C PNTs with outstanding electrical conductivity , a significant surface area , and effective catalytic activity by wrapping ordered mesoporous polypyrroles on the surface of 1D MoO 3 nanotubes using block polymers that self-assembled. FeMnO 3 hollow porous nanospheres have a large specific surface area, many electroactive sites, and excellent molecular/charge diffusion properties, which can be compounded with 1D ordered mesoporous nitride-rich carbon to solve the problem of poor electrical conductivity of metal oxides and to prevent the accumulation of 1D nanotubes. The composite showed excellent electrocatalytic activity towards rutin due to the synergistic interaction between Mo 2 C@C PNTs and FeMnO 3 . In the best case, rutin has a limit of detection (LOD) as low as 0.35 × 10 −9 M and a wide linear range (3 × 10 −9 to 3.1 × 10 −6 M). The sensor displays a remarkable level of selective response to rutin in the presence of other co-interfering species. Furthermore, it has superb repeatability and reproducibility. The sensor has shown outstanding recovery (95.7–104.1 %) in the practical viability of detecting rutin in beverages. This work not only provides a new candidate for the accurate detection of rutin but also provides insight into the exploration of advanced sensing materials.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Zhi Hu,Shucheng Liu,Nana Tang,Xuan Zhang,Jianming Pan
The main obstacles of recognition of flavoniods are low concentration and complexity of extract wastewater solutions. The structural and stability limitation of the sorbents is the significant reason for the difficulty in increasing the adsorption amount in the separation system. Hereon, a novel hierarchical-pore boronic acid-functionalized-MOFs based on macroporous organo/hydro copolymers (CPs@H-UiO-66-NH 2 @BA) were firstly fabricated through the triple sacrificial cross-linking strategy, which allowed the formation of multiple hydrogen bond networks (i.e. main crosslinked networks and weak crosslinked networks) at oil–water interface. Noticeably, the MOFs with polymer chain entanglement effects as a physical sacrificial cross-linking network, significantly enhancing the mechanical properties. The maximum equilibrium binding capacity of CPs@H-UiO-66-NH 2 @BA was 40.93 µmol g −1 for NRG at 308 K. Furthermore, it also showed superior regeneration capacity capability (only 7.81% loss) after six cycles. Meanwhile, they had a stronger selectivity towards NRG. It can effectively purify the low purity of the actual sample ground to 92.68%. It is anticipated that in-situ the introduction of nanoboronic-functionalized MOFs construction into copolymers would hold great promise in the selective capture of target flavonoids in actual samples.
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高影响因子
Foods
·
2023
·Vol. 12
·期 12
·2326
·
IF 5.2
Juan Liu,Yongling Lu,Bo Si,Anqi Tong,Yang Lu,Lishuang Lv
Acrolein (ACR) is a toxic unsaturated aldehyde that is produced during food thermal processing. Here, we investigated the synergistic effect of polyphenols in binary, ternary, and quaternary combinations on ACR by the Chou–Talalay method, and then explored the synergistic effect of cardamonin (CAR), alpinetin (ALP), and pinocembrin (PIN) in fixed proportion fromAlpinia katsumadaiHayata (AKH) combined with curcumin (CUR) in the model, and roasted pork using LC–MS/MS. Our results showed that their synergistic effect depended on the intensification of their individual trapping ACR activities, which resulted in the formation of more ACR adducts. In addition, by adding 1% AKH (as the carrier of CAR, ALP, and PIN) and 0.01% CUR (vs. 6% AKH single) as spices, more than 71.5% (vs. 54.0%) of ACR was eliminated in roast pork. Our results suggested that selective complex polyphenols can synergistically remove the toxic ACR that is produced in food processing.Keywords:acrolein;cardamonin;alpinetin;pinocembrin;Alpinia katsumadaiHayata;curcumin
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PubMed
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 5
·432
·
IF 3.7
Yuhao Sun,Zhenzhen Wang,Jing Dai,Ruyi Sha,Jianwei Mao,Yangchen Mao,Yanli Cui
In this work, spontaneously fermented and inoculation-fermentedAkebia trifoliatafruit Jiaosu (SFAJ/IFAJ) were compared. The key metabolites and antioxidant activities of SFAJ and IFAJ were tracked and tested during fermentation. The antioxidant effect of fermentedAkebia trifoliatafruit and the underlying mechanisms were explored using network pharmacology for the prediction and verification of the molecular targets and pathways of theAkebia trifoliatafruit’s action against oxidative stress. Furthermore, the results were verified by molecular docking and then investigated, based on a HepG2 cell model. The results of correlation analysis and principal component analysis (PCA) showed that there were significant positive correlations between the phenols, flavonoids, and terpenoids in SFAJ and IFAJ and their antioxidant activities. Network pharmacology and molecular docking analysis disclosed the antioxidation mechanism at the molecular level. In addition, both SFAJ and IFAJ were effective at alleviating oxidative stress in HepG2 cells. In particular, IFAJ performed better than SFAJ in protecting cells with an intracellular reactive oxygen species (ROS) level of 99.96 ± 4.07 U/mg prot, superoxide dismutase (SOD) activity of 41.56 ± 0.06 U/mg prot, catalase (CAT) activity of 91.78 ± 3.85 U/mg prot, and glutathione peroxidase (GSH-Px) activity of 39.32 ± 2.75 mU/mg prot in the IFAJ group. Collectively, this study revealed the changes in bioactive metabolite contents and the in vitro antioxidant activity during fermentation and investigated the protectiveness of SFAJ and IFAJ against oxidative stress within HepG2 cells, promoting the study of the antioxidant efficacy of IFAJ, thereby providing valuable reference data for the optimization of its preparation and the development of relevant products with health benefits.Keywords:Akebia trifoliatafruit;bioactive metabolites;antioxidant activity;network pharmacology;molecular docking;oxidative stress
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高影响因子
ANALYTICAL LETTERS
·
2021
·
IF 2.329
Ping-Rong Zhou,Zhang-Feng Tang,Ke-Su Wei,Ya Wan,Yu-Meng Gao,Yan-Mei Liang,Xiu-Fang Yan,Jun Bin,Chao Kang
Ultraviolet-visible absorption spectrometry is used for the determination of many inorganic, organic, and biological species. However, poor selectivity limits its applications for quantitative analysis in complex mixtures. In this work, its mathematical selectivity was explored using trilinear decomposition on spectral-pH measurements for the selective determination of two flavonoids in three Chinese herbal medicines. Through introducing a pH mode, second-order spectral pH data were constructed per sample. By using trilinear decomposition on the calibration and prediction samples, the rutin signals were extracted from lonicerae japonicae flos, perillae folium, and perillae fructus samples, respectively. Novel second-order calibration was developed for interference-free determination of rutin in these medicines followed by univariate regression of the decomposed intensity versus concentration. The predicted 95% confidence intervals for the concentration of rutin in these samples were 5.19 ± 0.16, 5.81 ± 0.10, and 7.00 ± 0.36 μg mL−1, respectively. These results were validated by high performance liquid chromatography (HPLC). Similarly, interference-free determination of isorhamnetin was achieved in these materials. By increasing the selectivity of ultraviolet-visible spectroscopy with trilinear decomposition, the requirements for sample preparation and chemical separation are reduced. The developed spectral pH second-order calibration allows direct analysis of complex samples by ultraviolet-visible absorption spectrometry due to the mathematical selectivity.
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高影响因子
Food Science and Technology
·
2021
·
IF 1.718
LI Ying,YANG Dan,CHEN Bo,CAO Hai-Yan,ZHANG Qing-Feng
The contents of total phenol and flavonoid of 27 edible plants as well as their antioxidative and digestive enzymes inhibitory activities were evaluated. The total phenol content of the 27 plants ranged from 1.28-46.80 mg gallic acid equivalent/g. DPPH, ABTS and reducing power assay explored that Rubus idaeus Linn, Hibiscus sabdariffa Linn. and Crataegus pinnatifida Bge. exhibited stronger antioxidant capacity among the 27 plants. 4 plants showed inhibitory percentage more than 50% on pancreatic lipase. 10 plants showed inhibitory percentage more than 50% on α-glucosidase. 3 plants simultaneously possess significant inhibitory activities on pancreatic lipase and α-glucosidase, which were Alpinia officinarum Hance, Ginkgo biloba Linn. leaves and Vitis vinifera Linn. Seeds. Strong positive correlation between total phenol content and antioxidative capacity were found. However, digestive enzymes inhibitory activity was less correlated with total phenol and total flavonoids content.
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高影响因子
CEREAL CHEMISTRY
·
2021
·Vol. 99
·期 3
·582-592
·
IF 1.984
Fan Wu,Fulin Tian,Zhou Jin,Jing Liu,Weiping Jin,Xuan Chen,Zhan Wang,Xiwu Jia,Benguo Liu,Lingyi Liu,Wangyang Shen
Background and objectives Pure wheat bran and wheat germ are the by-products of sieved flour. Pure wheat bran contains a substantial amount of dietary fiber, vitamins B and D, and other nutrients; lipases in the wheat germ induce lipid oxidation and break down vitamins. The present study used heat treatment to stabilize the wheat germ and then compounded it with the extruded pure wheat bran and compared it with the extruded bran. The antioxidant capacity and soluble polyphenols of the compounded wheat bran and extruded wheat bran were subsequently measured, to select a method to improve the antioxidant capacity of wheat bran. Findings The optimized heating temperature of wheat germ was 110°C. The half-maximal inhibitory concentration value of the total antioxidant capacity showed that the compounded wheat bran had a higher antioxidant capacity than the extruded wheat bran. It was hypothesized that the operations of extrusion and heat treatment increased the antioxidant capacity of compounded wheat bran. In addition, after the extrusion treatment, a variety of flavonoid glycosides in the wheat bran was released while the content of various phenolic acid types was decreased. However, the content of the phenolic acid types and flavonoids in the compounded wheat bran was increased. Hence, compounded wheat bran is more suitable for producing functional foods. Conclusion The results of this study revealed that extruded pure wheat bran and heat-treated wheat germ can effectively increase the antioxidant capacity and the content of the phenolic acid types and flavonoids; hence, it is more suitable for producing functional foods. Significance and novelty This study developed a fast and inexpensive detection method that uses a combination of heat and extrude to process compounded wheat bran, which provides the optimal process method for extending the yield and shelf life of wheat bran products.
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高影响因子
危险品化学品经营许可证（带存储）