FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Shan Yu,Yingying Ning,Jiaju Xu,Ruoxi Yang,Dan Zhao
The study used the mannanase-producing Lactobacillus casei HDS-01 as the probiotic component and combined it with konjac powder, which contains konjac glucomannan (KGM), to create a synergistic synbiotic. The aim was to investigate its probiotic potential and the changes in gut microbiota and short-chain fatty acids (SCFAs). The results indicated that KGM selectively stimulated the growth of L. casei HDS-01, confirming their synergistic effect. The survival rate of L. casei HDS-01 at pH levels of 2.5–5.5, bile salt concentration of 0–0.35 g/L, and NaCl concentration of 1–8 g/L reached more than 55 %, suggesting that L. casei HDS-01 can survive in the gastrointestinal tract. Furthermore, the colonisation ability of L. casei HDS-01 in the intestinal tract was significantly enhanced by KGM. The antidigestive property of konjac oligo-glucomannan (KOGM) in gastric juice was better than that of inulin, with 94 % of KOGM expected to reach the jejunum of the small intestine after entering the human digestive system. In vitro fecal fermentation results indicated that the L. casei HDS-01-KGM synbiotic effectively promote the proliferation of gut SCFAs-producing bacteria (2.43 × 10 8 CFU/mL) and increase SCFAs production. The contents of acetic acid (7.68 ± 0.04 μg/mL), propionic acid (6.72 ± 0.03 μg/mL) and butyric acid (4.37 ± 0.06 μg/mL) were higher than those of the other treatment groups. Meanwhile, L. casei HDS-01-KGM synbiotic could enhance the population of beneficial bacteria, such as Pediococcus while significantly reducing harmful bacteria, such as Shigella . These results demonstrate the synergistic effect of L. casei HDS-01-KGM synbiotic and establish the foundation for its application in the food industry
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Qing-Hua Liu,Liu Yang,Yue Wang,Hao-Ming Xiong,Cai-Yun Wang,Guo-Yuan Zhu,Li-ping Bai,Zhi-Hong Jiang,Wei Zhang
Polygonatum odoratum ( P. odoratum ) oligosaccharides (POO) are bioactive compounds with complex structures. Since the precise structure of POO remains undefined, their pharmacological development and clinical application have been hindered. This study developed a universal strategy to elucidate oligosaccharide structures of P. odoratum by combining Orbitrap high-resolution mass spectrometry (Orbitrap-HRMS) and enzymatic hydrolysis. Initially, monosaccharide constituents of free oligosaccharides (POO) and polysaccharides (POP) in P. odoratum were quantitatively analyzed. The accurate mass values, retention time, and sequence-specific fragment information facilitated initial characterization by comparing with the self-established oligosaccharide library based on these monosaccharides. Due to the limitations of MS in precisely identifying isomer structures, enzymatic hydrolysis was further employed to distinguish the glycosidic linkage and oligosaccharide type. Fructanase mixture, endo -1,4-β-mannanase, exo -1,3-β-D-glucanase, α-galactosidase and amyloglucosidase were used to identify hexose-based isomers, while α-L-arabinofuranosidase and endo -1,4-β-xylanase were used for pentose-based isomers. The study revealed that POO primarily consisted of fructooligosaccharides, and its isomers, such as 1-kestose, 6-kestose, and neokestose, were clarified. POP mainly comprised galactomannan, with the branching site at α-(1 → 6)-galactoside in POPO determined for the first time in P. odoratum . This strategy provides a straightforward methodology for analyzing plant oligosaccharides, establishing a structure analytical foundation for further pharmacological research and potential clinical applications.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Sai Yang,Ziteng Lian,Yifei Chi,Yaqi Tang,Mengmeng Wang,Jiexu Zhang,Xiaohong Tong,Huan Wang
The loose structure of natural soy protein isolate (SPI) gels limits their application in food nutrient delivery systems. This study investigated the effects of varying inulin (INU) concentrations combined with ultrasound treatment on the structural characteristics, stability, and riboflavin digestion and release of SPI-based hydrogels. Increasing INU concentration enhanced the textural properties and water-holding capacity (WHC) of the composite gels, converting free water into immobilized water. The addition of 2 % INU with ultrasound treatment (SU-2) markedly improved rheological properties, yielding a smoother, denser microstructure and increasing protein disorder. Molecular docking and molecular dynamics simulations indicated that the SPI-INU gel structure was stabilized primarily through hydrogen bonding and hydrophobic interactions. The modified gels improved riboflavin loading and stability, with SU-2 exhibiting the highest bioaccessibility (85.82 %) and chemical stability (76.01 %). This study contributes to the development of natural protein/polysaccharide gel carriers for targeted slow release of hydrophilic bioactives.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xueming Bao,Leilei Wu,Biaobiao Yan,Ying Liu,Man Zhou,Qiang Wang,Ping Wang
Transition metal oxides have shown great potential in catalytic indoor pollutant removals, but their applications are hindered by the poor aqueous dispersity, inhalable powder forms and concerning substrate adhesion. Herein, we proposed a simple technique to fabricate durable textile coatings with ‘brick-mud’ nanostructure of Ti 3 C 2 T x (MXene) and manganese dioxide (MnO 2 ). Viscosity-controllable inks were prepared by hybridizing inulin-templated MnO 2 nanoparticles with acid-etched MXene nanosheets at room temperature, establishing firm bindings through coordination bonds between transition metal cores and hydroxyls, covalent redox linkages of Mn/Ti vacancies and intermolecular hydrogen bonds. Meanwhile, the high flatness of MXene optimized the binder/catalyst-free deposition of MnO 2 onto cotton textiles, simplifying the finishing process and providing MXene@MnO 2 coatings with encouraging fastness through the aforementioned triple bridges. Consequently, the resulting textiles exhibit satisfactory photo-antibacterial activities against gram negative and positive bacteria (>99 % within 10 min), accompanying with promising formaldehyde (HCHO) removal ability (>83 % within 30 min) and encouraging ultraviolet-absorption property (<0.05 % of UVA). Summarily, the presented deposition strategy of ‘brick-mud’-like coatings of transition metal catalysts on textiles provides a reliable alternative for the fabrication of multifunctional home textiles to improve indoor living quality.
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2025
·
IF 1.9
Shuhan Yang,Shouqi Zhao,Yingying Ning,Dan Zhao
Previous studies have shown that co-culture methods can enhance exopolysaccharide production, no studies have reported this enhancement in co-cultures between Weissella confusa and Candida shehatae. This study developed an efficient co-culture system combining W. confusa XG-3 and C. shehatae (1:1 ratio), and employed response surface methodology to optimize both exopolysaccharide yield and properties. The initial co-culture produced 56.82 ± 0.12 g/L exopolysaccharide, a 1.63-fold increase over single culture. Optimization (sucrose: 105.97 g/L; yeast extract: 5.81 g/L; peptone: 6.24 g/L) further increased the yield to 115.66 g/L, a 2.04-fold improvement. The co-cultured exopolysaccharide had high water solubility (60.45 ± 0.70%) and water holding capacity (614.74 ± 9.26%). The co-cultured EPS showed significant antioxidant activity. At 5 mg/mL, the scavenging rates of ABTS, DPPH, hydroxyl, superoxide, H2O2 and total reducing power reached the highest 52.22 ± 0.47%, 50.79 ± 2.86%, 55.80 ± 2.56%, 36.05 ± 2.07%, 66.85 ± 0.30%, and 0.39 ± 0.01. It also had a superior effect on the proliferation of several probiotics compared with commercial prebiotics and exhibited high thermostability with a degradation threshold at 292.5 °C. These results demonstrate a novel, scalable strategy for producing multifunctional EPS with broad applications in the food industry.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Wenhua Jin,Fengyu Guo,Jiali Shi,Weibo Zhang,Hejing Tang,Senyang Hu,Zihao Wang,Pengjie Wang,Fazheng Ren,Hao Zhang,Yinhua Zhu
While dietary fiber is beneficial for health, excessive intake can cause adverse effects, such as bloating, abdominal pain, and diarrhea. The role of fiber molecular weight (Mw) and the underlying molecular mechanisms driving these effects remain unclear. Here, we screened dietary fibers of various types and Mws in mice and found that low-Mw xylan specifically induced acute watery diarrhea, whereas high-Mw xylan did not. We demonstrated using intestinal epithelial cells and a mouse model that low-Mw xylan acts as an intestinal osmotic stressor, triggering epithelial cell apoptosis via caspase-3 activation. This effect was accompanied by a restructuring of the cecal microbiota towards a gas-producing, pro-inflammatory and mucolytic profile. Our findings reveal a causal pathway in a mouse model linking low-Mw fiber intake to diarrhea through osmotic stress-induced apoptosis and microbial dysbiosis, establishing Mw as a critical parameter for evaluating fiber safety and formulating dietary recommendations.
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JOURNAL OF CATALYSIS
·
2025
·
IF 6.5
Xiaofei Liu,Daiyu Song,Yunyun Liu,Zhao Huang,Xinyao Wang,Kezhi Wang,Yuxin Yang,Yihang Guo
The cleavage of C–O bonds in biomass-derived carbohydrates is of great significance for the conversion of biomass to chemicals and fuels. We demonstrated that various F127-directed covalent triazine frameworks functionalized by sulfonic acid-based ionic liquids ([C 3 N][OTf]-FCTF) were synthesized by a two-step synthesis process. The interesting morphology of spherical nanofiber of the [C 3 N][OTf]-FCTF(0.25)-10 catalyst was obtained by carefully adjusting the usage of both F127 and ethanol in the synthetic system. The 5-hydroxymethylfurfural synthesis reaction parameters of were first optimized using the [C 3 N][OTf]-FCTF(0.25)-10 via response surface methodology. The [C 3 N][OTf]-FCTF(0.25)-10 exhibited high catalytic activity, and outperformed other CTF-based catalysts with different morphologies and commercial solid acids in the conversion of carbohydrates (e.g., fructose, inulin, sucrose and honey). This is due to the advantages of the loose spherical nanofiber, the rich N-containing units, the suitable Brønsted acid nature and the adjacent acid-base pairs on the surface of the catalyst. The high Brønsted acidity of [C 3 N][OTf]-FCTF(0.25)-10 played a positive role in the selective synthesis of levulinic acid from carbohydrates. The theoretical calculations demonstrated that discrepancies in electron cloud distribution between constructed acid-base pairs and N-rich groups in the catalyst could facilitate the cleavage of C–O bonds in fructose molecules. The Fukui function combined with experimental results elucidated the reaction pathways. The robust interaction between –[C 3 N][OTf] groups and frameworks ensured the excellent reusability of [C 3 N][OTf]-FCTF catalysts. This study will provide a novel approach for synthesizing CTF-based solid acids with controllable morphology, enabling the high-value utilization of carbohydrates.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Minghan Li,Jialin Guo,Jinxin Meng,Xueting Wang,Tianxu Pan,Dongyu Zhao,Hongye Li,Ruyi Gao,Ya Wang,Hechun Li,Songyang Li,Jiagan Wang,Haibin Huang,Nan Wang,Shuyuan Yu,Jiayao Guan,Mingxiao Liu,Chunfeng Wang,Guilian Yang
Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by inflammation and immune dysregulation, with the gut–liver axis playing a crucial role in immunometabolic balance. While dietary fibers show therapeutic potential, their mechanisms in T2DM remain unclear. This study investigates the effects of synbiotic intervention combining inulin and Lactiplantibacillus plantarum LPm77 on T2DM, focusing on the gut–liver axis. After 7 weeks of synbiotic treatment, significant improvements in hyperglycemia, insulin resistance, and hepatic steatosis were observed in T2DM mice. Inulin promoted L. plantarum LPm77 colonization, enhancing the gut microbiota balance and restoring the intestinal barrier function. It also regulated bile acid levels in the enterohepatic circulation. The synbiotic promoted tauro-ursodeoxycholic acid (TUDCA) biosynthesis, improving hepatic lipid metabolism and activating TGR5. TGR5 activation suppressed M1 macrophage polarization via the TGR5-TLR4-NF-κB pathway, reducing pro-inflammatory cytokines and increasing IL-10, thus alleviating systemic inflammation. This study highlights a novel therapeutic approach targeting immune-metabolic dysregulation in T2DM.
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Foods
·
2024
·Vol. 13
·期 12
·1799
·
IF 4.7
Gansheng Tan,Zhouwei Duan,Guanghua Xia,Tian Xin,Ling Yang,Feng Liu,Hui Xie
The polysaccharides were extracted from the leaves ofMallotus oblongifolius(MO) using an ultrasonic-assisted extraction method in this study. The main variables affecting the yield of polysaccharides extracted fromMallotus appallatus(MOPS) were identified and optimized while concurrently investigating its antioxidant capacity, hypoglycemic activity, and digestive properties. The results indicated that the optimal ultrasound-assisted extraction of MOPS involved an ultrasound power of 200 W, a liquid-to-solid ratio of 25:1 (mL:g), an extraction temperature of 75 °C, and an ultrasound time of 45 min, leading to an extraction yield of (7.36 ± 0.45)% (m/m). The MOPS extract exhibited significant scavenging activity against DPPH and ABTS radicals with IC50values of (25.65 ± 0.53) μg/mL and (100.38 ± 0.38) μg/mL, respectively. Furthermore, it effectively inhibited the enzymatic activities of α-glucosidase and α-amylase with IC50values of (2.27 ± 0.07) mg/mL and (0.57 ± 0.04) mg/mL, respectively. The content of MOPS remained relatively stable in the stomach and small intestine; however, their ability to scavenge DPPH radicals and ABTS radicals and exhibit reducing power was attenuated, and the inhibition of α-amylase and α-glucosidase activity was diminished. In conclusion, the ultrasonic extraction of MOPS showed feasibility and revealed antioxidant and hypoglycemic effects. However, the activities were significantly reduced after gastric and small intestinal digestion despite no significant change in the MOPS content.
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