SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yuan Chen,Tongming Chen,Xiaoju Wu,Guowei Yang
As delafossite type of metal oxide materials, CuMnO 2 nanoflakes show great promise for optoelectronics applications due to their excellent optical, electric and multiferroic properties. Here, we for the first time demonstrate that CuMnO 2 nanoflakes possess intrinsic activity of mimicking enzymes of peroxidase, oxidase and catalase . Through modulating the pH value, CuMnO 2 nanoflakes can catalyze the reduction of oxygen (O 2 ) and hydrogen peroxide (H 2 O 2 ) at acidic environment, and the decomposition of H 2 O 2 to produce O 2 at alkaline pH. The mechanism of their peroxidase-like activity has been proposed as the generation of hydroxyl radical ( OH). Based on the colorimetric reaction of CuMnO 2 nanoflakes and the hindrance of the peroxidase-like activity in the presence of H 2 O 2 and l -cysteine, a simple platform for l -cysteine detection with low detection limit of 11.26 μM has been developed. Compared with other bimetallic oxides, CuMnO 2 nanoflakes exhibit higher sensitivity and lower detection limit towards l -cysteine. Surprisingly, the proposed method for l -cysteine estimation can also be used in milk samples. These findings pave the way for further investigation about the catalytic activity of CuMnO 2 nanoflakes and make them a promising candidate for biomedical applications.
研究领域:
生物化学
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纳米医学
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材料科学
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2018
·
IF 2.616
Jinsha Huang,Wei Zhuang,Lei Ge,Kai Wang,Zhenfu Wang,Huanqing Niu,Jinglan Wu,Chenjie Zhu,Yong Chen,Hanjie Ying
Surface amine modification could not only improve the microenvironment near the active sites of enzyme, but also enhance the multi-point chemical crosslinking between the enzyme and carrier. The linear structured polymer of ε-poly - l - lysine (EPL) is an ideal donor of amino with much more exposed on the surface for enzyme attachment. Analysis of the result of dynamic light scattering (DLS) and circular dichroism (CD) demonstrated the favorable electrostatic interactions and negligible impact on the conformation of enzymes, Glucose Oxidase and Catalase (GOx&CAT). Titration and dissociation curve together with Zeta-potential characterization revealed that enzymes (EPL@GOx&CAT) under the protection of EPL had more stable structure and better activity and stability in acidic reaction environment. Meanwhile, the activity recovery of immobilized EPL@GOx&CAT increased to 1.56 times higher than that of GOx&CAT, and the acid resistance was improved by 1.44 times with the optimum pH shifting to acidic by 0.50 unit. The substrate affinity was raised with the decrease of K m from 5.98 to 4.35 mg mL −1 . Thus, the gluconic acid production in low pH system could be increased by pH-engineering of the enzyme microenvironment via conjugation with positively charged polyelectrolyte.
研究领域:
生物化学
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酶工程
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Jinghui Wang,Renliang Huang,Wei Qi,Rongxin Su,Zhimin He
Despite the rapid development of nanozyme , it is still challenging to develop multifunctional nanozyme with high oxidase-like activity. In this work, a novel type of amorphous imidazole-Cu nanozyme (I-Cu) with high laccase- and catecholase-like activity was prepared by the water induced precipitation of Cu 2+ and imidazole to mimick the N-Cu coordinated environment in their active site. I-Cu shows similar K m but 3.7-fold higher V max than laccase at the same mass concentration. The possible catalytic mechanism of I-Cu nanozyme, which is composed of substrate binding, substrate oxidation , oxygen binding and oxygen reduction, is proposed. In addition, it also displays high stability under various pH, temperature, long-term storage and high salinity . The oxidation efficiency of I-Cu nanozyme for environmental phenolic pollutants (2,4-dichlorophenol) is 91.8% at 10 h, which is higher than that of laccase (68.8% at 10 h). Furthermore, the colorimetric and smart phone detection of dopamine by I-Cu nanozyme is developed with the detection limit of 0.412 μM. Therefore, we expect this amorphous nanozyme is promising in the various application of bioremediation and biosensor .
研究领域:
生物化学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 8
·4103-4112
·
IF 3.3
Ke Yang,Guoning Chen,Lu Wang,Miao Guo,Jiameng Xu,Yirong Ma,Zhimin Luo,Aiguo Zeng,Qiang Fu
Co-immobilization strategies are available to improve the catalytic activity, stability and reusability of enzymes. It has been frequently applied in recent year in reactions involving multi-step catalysis to optimize the performance. In this study, a zeolitic-imidazolate framework 8 (ZIF-8)-based nanoreactor (Chox&HRP@ZIF-8) including cholesterol oxidase (Chox) and horseradish peroxidase (HRP) was developed successfully. The Chox&HRP@ZIF-8 was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy, respectively. The as-prepared materials were used as a biocatalytic system to realize the cascade reaction initiated by Chox and HRP. Next, the investigation of the catalytic activity and stability of Chox&HRP@ZIF-8 was performed in extreme environments. The results showed that the stability of the prepared Chox&HRP@ZIF-8 was significantly improved compared with that of free enzymes. In addition, the assay method for cholesterol detection developed based on Chox&HRP@ZIF-8 as the biocatalytic system showed high selectivity and good interference resistance. It was successfully applied to the determination of cholesterol in mice serum. It is worth mentioning that the relative activity of co-localized Chox&HRP remained above 60% after 5 cycles for cholesterol detection, confirming the feasibility of the multi-enzyme immobilization strategy using ZIF-8 as a carrier in the cascade reaction.
研究领域:
生物化学
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纳米生物学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Shiqi Xu,Songtao Wang,Ling Guo,Yuqin Tong,Lina Wu,Xingxu Huang
CRISPR-based detection was often based on the target preamplification to realize the high sensitivity. Here, we prepared a CRISPR-Cas12a system for the colorimetric detection of lead ion (Pb 2+ ) based on the assistance of DNAzyme and nanozyme instead of preamplification. The recognition between GR-5 DNAzyme and Pb 2+ could trigger the CRISPR-Cas12a system. MnO 2 nanozymes connected with magnetic beads through single stranded DNA were prepared as the colorimetric signal probes and catalyst of CRISPR-Cas12a system for the strong oxidase-like activity inducing the color change of 3,3′,5,5′-tetramethylbenzidine. The nanozyme-catalysed CRISPR-Cas12a system could be used to detect Pb 2+ through the color change with high specificity and sensitivity. The linear range of this approach was 0.8 nM–2500 nM, with a limit of detection of 0.54 nM. This method was applied for the detection of the Pb 2+ in food samples indicating good accuracy and anti-interference ability.
研究领域:
生物传感器
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分析化学
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环境科学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xinxin Ren,Kaiyue Hu,Lingxia Qin,Di Wu,Zhiyong Guo,Sui Wang,Yufang Hu
The investigation on invertase (INV) and glucose oxidase (GOx)-dominated biological process offers a new opportunity for the development of clinical diagnosis and prognostic treatment. Herein, a ZnO nanoflowers (ZnONFs)-assisted DNAzyme-based electrochemical platform for INV- and GOx-dominated biosensing is proposed by the change of pH in microenvironment. In this strategy, INV can usually catalyze the dissolution of sucrose to generate glucose, and glucose is then consumed by GOx to produce H 2 O 2 and gluconic acid , in which ZnONFs can be effectively etched into free Zn 2+ ions. Subsequently, the released Zn 2+ ions have a shearing action for Zn 2+ -specific DNAzyme, thus triggering hybridization chain reaction along with the imbedding of methylene blue . The excellent electrochemical signals illustrate the method can be employed well for testing sucrose, INV and GOx with a low detection limit (0.019 μM, 0.047 mU/mL and 0.012 mU/mL, respectively). Finally, a series of basic and advanced logic gates (YES, AND, INHIBIT, and AND-AND-INHIBIT) in the biological process are constructed with different logic inputs, providing a valuable platform for the establishment of advanced molecular devices for bioanalysis and clinical diagnostics.
研究领域:
生物化学
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分子生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
DanDan Yang,Jingqi Chen,Yuting Huang,Ganghui Chen,Xueliang Liu,Xuan Wang,Like Yang,Zhaohui Li,Jie Hu,Qi Zhou,Jia Ge,Yu Yang
The development of cost-effective and easy-to-use strategies for the detection of ascorbic acid (AA) and acid phosphatase (ACP) is in high demand but challenging. Thus, we report a novel colorimetric platform based on Fe–N/C single atom nanozyme with efficient oxidase mimicking activity for their highly sensitive detection . The designed Fe–N/C single atom nanozyme can directly oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue oxidation product (oxTMB) in the absence of H 2 O 2 . In addition, L-ascorbic acid 2-phosphate can be hydrolyzed to ascorbic acid in the presence of ACP, which inhibits the oxidation reaction and results in a significant bleaching of the blue color. Based on these phenomena, a novel colorimetric assay with high catalytic activity was developed for the determination of ascorbic acid and acid phosphatase with detection limits of 0.092 μM and 0.048 U/L, respectively. Notably, this strategy was successfully applied to the determination of ACP in human serum samples and evaluate ACP inhibitors, indicating its potential as a valuable tool for clinical diagnosis and research.
研究领域:
生物化学
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医学诊断
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jun Liang,Zhixi Zhang,Qiang Zhang,Yaqian Bian,Kunlong Yi,Wenbin Zhou,Weiyi Su,Yuqi Hu,Xiong Yu,Honghai Wang
Driven by global environmental awareness, biocatalysts are playing an increasingly important role in industrial chemistry . Enzymatic reactive distillation (ERD) combines the high efficiency of enzymatic reaction and distillation separation to reduce the limiting effects of reaction equilibrium, product inhibition, and the difficulty of product recovery due to its thermodynamic constraints. The lipase CALB was immobilized and coated on the θ-ring by sol-gel method. The synthesis of n-butyl acetate by transesterification reaction was selected as the model reaction in the ERD process, and a laboratory scale column was constructed. The Rate-based RadFrac model in Aspen Plus was used to describe the ERD. The results showed that the enzyme-loaded packing showed high long-term stability after 120 days in the reaction system. The deviation of the ERD experiment and simulation results was less than 10 %, which verifies the reliability of simulation. After the parameter sensitivity optimization on the simulation, compared with the batch reactor, the ERD process increased the reactant conversion from 60 % to 94.74 %. This study established a complete process from enzyme immobilization to ERD process, which provided a theoretical basis for the establishment and optimization of ERD process.
研究领域:
生物化学工程
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工业化学
·
过程工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 24
·17376
·
IF 5.6
Mingyu Xue,Xiaohong Xia,Yadi Deng,Fei Teng,Shiyue Zhao,Hui Li,Dejun Hao,Wei-Yi Chen
Alpha-pinene is one of the main defensive components in conifers.Monochamus alternatus(Coleoptera: Cerambycidae), a wood borer feeding on Pinaceae plants, relies on its detoxifying enzymes to resist the defensive terpenoids. Here, we assayed the peroxide level and GST activity ofM. alternatuslarvae treated with different concentrations of α-pinene. Meanwhile, agstgene (MaGSTe3) was isolated and analyzed. We determined its expression level and verified its function. The results showed that α-pinene treatment led to membrane lipid peroxidation and thus increased the GST activity. Expression ofMaGSTe3was significantly upregulated in guts following exposure to α-pinene, which has a similar pattern with the malonaldehyde level. In vitro expression and disk diffusion assay showed that the MaGSTe3 protein had high antioxidant capacity. However, RNAi treatment of MaGSTe3 did not reduce the hydrogen peroxide and malonaldehyde levels, while GST activity was significantly reduced. These results suggested MaGSTe3 takes part in α-pinene adaptation, but it does not play a great role in the resistance ofM. alternatuslarvae to α-pinene.Keywords:glutathione S-transferase;detoxification enzymes;monoterpene
研究领域:
昆虫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Pengjuan Ni,Yujing Sun,Haichao Dai,Wangdong Lu,Shu Jiang,Yilin Wang,Zhen Li,Zhuang Li
In this paper, a novel, convenient and sensitive colorimetric assay for acetylcholinesterase (AChE) activity and its inhibitor screening is successfully proposed based on the peroxidase-like activity of Prussian blue nanocubes (PB NCs). PB NCs can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to develop a blue color and an absorption peak centered at 652 nm. AChE mediates the hydrolysis of acetylthiocholine (ATCh) to yield a reducing agent thiocholine (TCh) that cause the reduction of oxidized TMB and it can also chelate with Fe 3+ , both of which result in a blue color fading and a decrease of the absorbance. The generation of TCh is inhibited and the absorbance intensity is recovered after the introduction of AChE inhibitor. Consequently, the assay is also utilized in AChE inhibitor screening. The PB NCs-H 2 O 2 -TMB based platform is highly sensitive for AChE activity sensing with a low detection limit of 0.04 mU/mL. In addition, this assay has great potential in discriminatively determining AChE over other enzymes. The proposed method is fairly novel, simple and sensitive, which may pave the way for the detection of other hydrolytic enzyme activities with properly selected substrates.
研究领域:
生物分析化学
·
酶学
·
纳米生物技术
·
药物筛选
·
分子诊断
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xiao-Yu Han,Liu Zhang,Jie Yu,Wen-Jing Sun,Mei-Jing Chang,Gao-Ying Zhi,Dong-Hao Zhang
Here, glucose oxidase (GOD) is connected to Fe3O4 nanozyme by using epoxy cross-linkers as a spacer arm to construct a GOD-nanozyme artificial enzyme cascade system that is easy to separate magnetically. The dual-enzyme system can regulate the distance between GOD and nanozyme by changing the chain length of the cross-linker. The optimal interenzyme spacing of 2 nm has led to a significant increase in the cascade activity of the dual-enzyme system to 1400 U/mg, which is 2.5 times higher than the cascade activity of the free two enzymes. The GOD-nanozyme cascade system is applied to glucose assay, which has high sensitivity and selectivity for glucose with the lowest detection limit of 0.1 mM. Even in a system where the concentration of interfering substances is 10 times higher than that of glucose, the GOD-nanozyme cascade system can still specifically recognize glucose. The dual-enzyme system is used to establish a visual analysis device for glucose detection. The design of this visual analysis device provides a new strategy for the visual detection of glucose, which is of great significance for promoting the development of bioenzyme-nanozyme cascade systems in bioanalysis.
研究领域:
生物分析
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纳米技术
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酶学
·
生物传感器
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2021
·
IF 4.354
Chunfang Yang,Chunshuai Huang,Chunyan Fang,Liping Zhang,Siqiang Chen,Qingbo Zhang,Changsheng Zhang,Wenjun Zhang
Inactivation of the flavoenzyme-encoding gene flsO1 in fluostatin biosynthesis led to the isolation of four new angucyclinone derivatives (11, 12, 14, and 15), among which fluostarenes A (14) and B (15) featured the unprecedented 6/6/5/6/6 pentacyclic skeleton with fusion of a benzo[b]fluorene and a six-membered lactone ring. Both 14 and 15 were putatively generated via quinone methide-mediated nonenzymatic reactions. Fluostarene B (15) exhibited cytotoxicity against several cancer cell lines with IC50 values ranging from 7 to 10 μM. Fluostarenes A (14), B (15), and PK1 (16) showed α-glucosidase inhibition activity with IC50 of 0.89, 1.58, and 0.13 μM, respectively. Successful complementation of the ΔflsO1 mutant with alpK from kinamycin biosynthesis suggests that FlsO1 should function equivalently to AlpK as a putative C-5 hydroxylase.
研究领域:
药理学
·
生物化学
·
分子生物学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）