Journal of Nanomaterials
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2014
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IF 1.611
Wang Li,Fa-gen Li,Jun Wang,Guo-meng Zhao
Uniform magnetic hollow nanospheres (GdNi2, Co5Gd) coated with Gd2O3 have been successfully prepared on a large scale via a urea-based homogeneous precipitation method using silica (SiO2) spheres as sacrificed templates, followed by subsequent heat treatment. Nitrogen sorption measurements and scanning electron microscope reveal that these hollow-structured magnetic nanospheres have the mesoporous shells that are composed of a large amount of uniform nanoparticles. After reduction treatment, these nanoparticles exhibit superparamagnetism that might have potential applications in medicine. Furthermore, the developed synthesis route may provide an important guidance for the preparation of other multifunctional hollow spherical materials.
研究领域:
材料科学
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纳米技术
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生物医学工程
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DOI
高影响因子
Journal of Nanomaterials
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2017
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IF 1.871
Juan Zhang,Huanhuan Weng,Xiangwan Miao,Quanming Li,Siqi Wang,Huifen Xie,Tao Liu,Minqiang Xie
A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed. The core shell nanocomposites ([email protected]) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction. The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate. Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency. Transmission electron microscopy image of [email protected] nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm. Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml. Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases. Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro. The results suggested the potential application of [email protected] as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.
研究领域:
纳米技术
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癌症治疗
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药学
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生物医学工程
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产品关联
DOI
高影响因子
Frontiers in Pharmacology
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2025
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IF 4.8
Xiaoli Deng,Chunjie Hu,Lin Li,Guixian Yang,Chikun Li,Wanxun Zhang,Guangyu Cheng
Background Lung cancer is a leading cause of cancer-related deaths, with cisplatin being a cornerstone of treatment. However, resistance to cisplatin presents a significant challenge. β-elemene, a natural compound, has demonstrated potential to reverse cisplatin resistance. LncRNA LINC00511 has been implicated in cisplatin resistance through its role in activating the PI3K/AKT/mTOR pathway, which supports tumor survival and proliferation. Purpose This study aims to investigate the mechanism by which β-elemene overcomes cisplatin resistance in lung cancer by regulating LINC00511. Methods Human lung adenocarcinoma cells (A549 and A549/DDP) were treated with β-elemene and cisplatin. Cell proliferation and apoptosis were assessed using CCK-8, EdU staining, and flow cytometry. LINC00511 expression was measured by qRT-PCR, and protein levels of PI3K, AKT, and mTOR were evaluated via Western blot. A xenograft model was used to confirm in vivo effects. Results β-elemene significantly enhanced cisplatin-induced apoptosis in A549/DDP cells, reduced LINC00511 expression, and inhibited the PI3K/AKT/mTOR pathway. LINC00511 knockdown further potentiated these effects, both in vitro and in vivo . Xenograft models confirmed the enhanced anti-tumor effects of the combination treatment. Conclusion β-elemene overcomes cisplatin resistance in lung cancer by downregulating LINC00511 and inhibiting the PI3K/AKT/mTOR pathway. These findings propose a promising therapeutic strategy for treating cisplatin-resistant lung cancer.
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2020
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IF 6.155
Ruifang Gao,Jin Li,Rui Shi,Yang Zhang,Fuzhou Ouyang,Ting Zhang,Lihua Hu,Guoqiang Xu,Jian Liu
Protein phosphorylation is one of the most important post-translational modifications. It is an active research area to study phosphoproteomics for discovery of disease biomarkers and druggable targets. Here we report the development of superparamagnetic Fe 3 O 4 @mZrO 2 core-shell microspheres with mesoporous structures for highly efficient enrichment of phosphopeptides. We have demonstrated that the mesoporous ZrO 2 layer dramatically improves the selective enrichment of phosphopeptides. Our approach allows for in-situ elution and sensitive identification of both mono-phosphorylated and multi-phosphorylated peptides in MALDI-TOF mass spectrometry, with the detection limit of down to the femtomole range. The target phosphopeptides can reliably be enriched for MS analysis from various complex samples including the spiked protein digests and tumor cell lysates. The Fe 3 O 4 @mZrO 2 core-shell microspheres promise a useful tool for phosphoproteomics by allowing for highly efficient and selective enrichment of the crucial signaling regulators in a low abundance.
研究领域:
生物化学
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蛋白质组学
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医学研究
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DOI
高影响因子
CURRENT MEDICINAL CHEMISTRY
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2024
·Vol. 32
·期 33
·7357-7376
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IF 3.5
Jinna Di,Wei Bo,Chun Wang,Chunying Liu
IntroductionThe effectiveness of therapy is strongly impacted by the resistance of lung cancer cells to cisplatin. The objective of this research is to characterize the impact of ailanthone on cisplatin resistance in non-small cell lung cancer (NSCLC) and examine any potential in vivo and in vitro molecular pathways.MethodsFollowing treatment of A549/DDP cells with ailanthone and cisplatin, cell survival and apoptosis were measured using flow cytometry and Cell Counting Kit-8 (CCK8) assays, respectively. mRFP-GFP-LC3 adenovirus transfection was used to track autophagy, and protein expression levels were analyzed by western blotting. Hematoxylin and eosin (H&E) staining was used after the organs of the mice were removed for in vivo investigations. In A549/DDP cells, ailanthone and cisplatin together induced autophagy and apoptosis in a dose and time-dependent manner (p< 0.05). After receiving combined treatment with ailanthone and cisplatin, the expression levels of cleaved caspase-3, Bcl-2, cleaved PARP, Beclin 1, LC3B-II were considerably elevated by inhibiting the PI3K/AKT/mTOR signaling pathway.ResultsOur findings show that ailanthone, without causing adverse effects in vivo, greatly suppressed the growth of A549/DDP-grafted tumors and improved the anti-tumor efficaciousness of cisplatin.ConclusionAccording to this study, ailanthone may increase sensitivity to cisplatin and promote autophagy and death in NSCLC A549/DDP cells through the signaling pathway of PI3K/AKT/mTOR.
研究领域:
肿瘤学
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分子生物学
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药理学
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转化医学
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细胞信号转导
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2016
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IF 3.138
Jun-Jun Shi,Jian-Guo Zhang,Yu-Han Sun,Jie Qu,Ling Li,Chandan Prasad,Zhao-Jun Wei
The sequential extraction of peony seed dreg polysaccharides (PSDP) with hot buffer (HBSS), chelating agent (CHSS), dilute alkaline (DASS) and concentrated alkaline (CASS) yielded four different polysaccharide fractions. Based on their absorptions at 3600–3200 cm −1 and 1200–800 cm −1 , these fractions were confirmed to be polysaccharides . The properties of four PSDPs displayed some slight differences. The CASS showed the highest peak temperature and endothermic enthalpy. The emulsifying activity and emulsifying stability of four PSDPs exhibited a dose-dependent pattern; HBSS showed the highest emulsifying activity, and CHSS displayed the longest emulsifying stability. The four PSDPs also exhibited wide variations in their antioxidant activities. For example, i) CASS showed the highest DPPH radical scavenging activity, reducing power and ABTS radical scavenging activity; ii) HBSS exhibited the highest hydroxyl radical scavenging activity, and iii) CHSS displayed the higher ferrous ions chelating ability than others.
研究领域:
食品科学
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天然产物化学
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碳水化合物化学
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功能食品
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抗氧化研究
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Haili Wei,Shiyu Xia,Xueqing Sang,Juliana Gaithan Kauno,Zhiyong Dong,Yue Zhang,Jinfang Nie,Yali Yuan,Guobao Xu,Wenxin Niu
The “Lady Tasting Tea” is a classic experiment in statistics, often celebrated for its contribution to the field, though the lady’s extraordinary taste sensitivity is less frequently acknowledged. Herein, we developed a highly sensitive nanozyme-based artificial taste sensor, which differentiates teas and brewing processes, similar to the “Lady Tasting Tea” experiment. The sensor employs PdSb concave nanocubes, which exhibit enhanced peroxidase activity due to the oxophilicity of Sb, to construct a cross-responsive sensing platform that simultaneously identifies tea polyphenols and authenticates complex blank tea samples. With its multi-differentiation ability to simulate human taste, this platform objectively demonstrates the flavor differences in milk tea based on the order of milk and tea addition, providing reliable data to distinguish taste differences without subjective interference. Overall, this work presents a new approach for designing nanozyme-based sensing platforms to simulate human taste.
研究领域:
生物化学
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食品科学
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纳米技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Jialing Tang,Xiaoli Qin,Ritva Repo-Carrasco-Valencia,Xiai Yang,Yanchun Deng,Chunsheng Hou,Xiushi Yang
Jute ( Corchorus olitorius L.) leaves have long been consumed as an edible vegetable in Asia and Africa, which are underexplored sources of bioactive polysaccharides. This study employed sequential solvent extraction (water, oxalate, acid, alkali) to isolate four novel polysaccharide fractions (WJP, OJP, HJP, AJP) and evaluated their structural, functional, and antioxidant properties. Polysaccharides were characterized for chemical composition, monosaccharide profile, molecular weight, and microstructure. Functional properties (oil/water holding, emulsification, rheology) and DPPH/ABTS scavenging activities were assessed against commercial citrus pectin (CP). All polysaccharide fractions were RG-I dominant with solvent-dependent features. WJP had the highest M w (3.30 × 10 6 Da) and viscosity; OJP showed peak emulsification linked to high DE (27.79 %); HJP exhibited superior emulsion stability from extensive branching; AJP contained abundant protein (12.70 %) and phenolics (59.46 mg GAE/g), driving exceptional antioxidant capacity. Functionally, jute leaf polysaccharides surpassed CP in OHC, WHC, and emulsification. Outperforming antioxidant activities of AJP and WJP correlated with protein-phenolic content and structural features. Solvent-directed sequential extraction yields jute polysaccharides with customizable functionality. OJP (emulsifier), WJP (thickener), and AJP/WJP (antioxidants) offer sustainable, high-performance alternatives to commercial hydrocolloids, contingent on further purification.
研究领域:
食品化学
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植物生物化学
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生物聚合物科学
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营养科学
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产品关联
DOI
PubMed
高影响因子
CRYSTAL GROWTH & DESIGN
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2024
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IF 3.2
Yujiao Li,Yunan Zhang,Qi An,Jingwen Shi,Lixin Liu
Based on the study of the effect of positional isomerism of coformer functional groups on the cocrystallization and physicochemical properties of the active pharmaceutical ingredients, the impact of cis–trans isomeric butenedioic acid as coformers on the conformation, crystal structure, and its physicochemical properties of marbofloxacin was further explored. In this work, fumaric acid (FA) and maleic acid (MA) with different configurations were chosen as coformers to synthesize the pharmaceutical salt cocrystal (MBF-FA-H2FA) and salt (MBF-MA) of marbofloxacin (MBF), and their structures were fully characterized. Significant differences between the conformations of marbofloxacin in the salt cocrystal and in salt were found. In the salt cocrystal, the N atom of the piperazine group from marbofloxacin is coplanar with the pyridone ring, and the whole is straight like fumaric acid, whereas the marbofloxacin piperazine group in the salt is bent like the maleic acid configuration. Furthermore, the conformational variability of marbofloxacin in the salt cocrystal and the salt resulted in different crystal structures and opposite physicochemical properties. Notably, both multicomponent crystals have a surface hydrophilic intercalation structure. However, the salt cocrystal and salt exhibited different solubility and permeability. Specifically, the MBF-MA salt showed improved solubility and permeability, while the MBF-FA-H2FA salt cocrystal showed a decreased solubility and permeation rate compared to MBF. In addition, in vitro bacterial inhibitory activity assays indicated that the MBF-MA salt has stronger inhibitory activity against Gram-negative and Gram-positive bacterial strains than the MBF-FA-H2FA salt cocrystal and pure MBF.
研究领域:
药学
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晶体学
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生物化学
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产品关联
DOI
高影响因子
Nanomaterials
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2024
·Vol. 14
·期 10
·852
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IF 4.4
Jun Chen,Mengdong Tu,Mengying Xu,Depeng Gong,Xi Li,Chaocan Zhang
In this paper, we designed and investigated a reduction-based method to synthesize controllably monodisperse superparamagnetic nano Fe3O4colloidal clusters for magnetically responsive photonic crystals. It was shown that the addition of ascorbic acid (VC) to the system could synthesize monodisperse superparamagnetic nano Fe3O4and avoided the generation of γ-Fe2O3impurities, while the particle size and saturation magnetization intensity of nano Fe3O4gradually decreased with the increase of VC dosage. Nano Fe3O4could be rapidly assembled into photonic crystal dot matrix structures under a magnetic field, demonstrating tunability to various diffraction wavelengths. The nano Fe3O4modified by polyvinylpyrrolidone (PVP) and silicon coated could be stably dispersed in a variety of organic solvents and thus diffracted different wavelengths under a magnetic field. This is expected to be applied in various scenarios in the field of optical color development.
研究领域:
材料科学
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光学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Bin Wang,Yazhen Yao,Wanli Wang,Yujie Xu,Yi Wan,Yi Sun,Qiang Li,Han Hu,Mingbo Wu
Hard carbon (HC) has emerged as a highly promising anode material for sodium ion batteries, drawing tremendous interest in producing this material with low-cost and easily accessible precursors. The determination of the crucial parameters of precursors influencing the formation of key structures, such as closed pores, in the HC is of paramount importance. Considering the potential role of free radicals in the structural evolution of the precursors, we, for the first time, delve into the impact of radical species on the development of closed pores by electron paramagnetic resonance spectroscopy, with petroleum asphalt as the model system. Our findings reveal that carbon centred radicals, with the g value close to that of the free electron (2.0023), exhibit a propensity to form long-range, well-ordered graphitic structures with lower sodium storage capacity. Conversely, the deliberately incorporated oxygen radicals with the g value over 2.005 require a higher energy for ordering the graphitic structures, leading to the creation of closed pores. As a result, the optimal sample showcases a four-fold increase in plateau capacity for sodium ion storage due to the pore filling process. Our research underscores the pivotal role of employing electron paramagnetic resonance spectroscopy studying the critical structural evolution of functional carbon materials.
研究领域:
材料科学
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电化学
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能源存储
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产品关联
DOI
PubMed
高影响因子
American Journal of Cancer Research
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2024
·Vol. 14
·期 12
·5752
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IF 3.6
Xiaobo Cai,Min Cao,Qingliang Yang,Xiazhen Yu,Xin-Hua Feng,Robert Yongxin Zhao
Gastric cancer is a common malignant tumor with high incidence and mortality. The overexpression of Human epidermal growth factor receptor 2 (HER2) is associated with increased metastatic potential and poor clinical outcome in gastric cancer. Despite the proven clinical response rates of approved HER2-targeted therapies, including Trastuzumab combined with chemotherapy, their limited long-term clinical benefits and inevitable disease progression still pose significant challenges to the clinical treatment of gastric cancer. Hence, exploring novel strategies to enhance therapeutic outcomes for HER2-positive patients is extremely crucial and urgent. Here, we reported that DX126-262, a novel HER2-targeted antibody-drug conjugate, generated by conjugating a potent Tubulysin B analogue (Tub-114) to humanized anti-HER2 monoclonal antibody, exhibited a significant synergistic inhibitory effect with both Cisplatin and 5-FU in HER2-positive gastric cancer NCI-N87 cells. Moreover, the triple-drug combination strategy of DX126-262 combined with Cisplatin and 5-FU showed much better in vitro and in vivo therapeutic efficacy than monotherapy or double-drug combination (Cisplatin plus 5-FU) or first-line standard-of-care (SOC, Herceptin plus Cisplatin and 5-FU), and comparable or even superior in vivo efficacy than third-line SOC (DS-8201a) in NCI-N87 cells and xenograft models. Meanwhile, the triple-drug combination therapy did not exhibit superimposed toxicity. Taken together, our findings provide compelling evidence that DX126-262 in combination with Cisplatin and 5-FU exerts synergistic antitumor activity and is a promising strategy to improve the clinical efficacy of HER2-positive advanced or metastatic gastric cancer.
研究领域:
肿瘤学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）