NEUROSCIENCE
·
2017
·
IF 3.277
Tanzeel Huma,Xintian Hu,Yuanye Ma,Andrew Willden,Joshua Rizak,Muhammad Shahab,Zhengbo Wang
Embryonic stem cells (ESCs) have enormous potential as novel cell-based therapies, but their effectiveness depends on stem cell differentiation and specific signaling regulators, which remain poorly understood. In this study, a kisspeptin peptide (KP-10) was used at different dosages to determine whether rhesus macaque-derived tau GFP-Lyon ES cells underwent kisspeptin-specific neuronal differentiation. It was found that KP-10 exhibited an anti-proliferative effect on the cells and led to morphological changes and cellular differentiation consistent with neuronal stem cell (NSC) development. The cells differentiated into Gonadotrophin Releasing Hormone (GnRH) neuronal-like cell types in response to the KP-10 treatment. There has been a previously observed connection between kisspeptin signaling, GnRH neurons and their dysfunction found in congenital disorders like idiopathic hypogonadotropic hypogonadism (IHH). Although therapeutics are a still a far-off goal, the formation and development of GnRH-positive neuronal-like cells following the application of KP-10 to Lyon NSC cells opens the door for future NSC-based therapies to treat specific reproductive disorders.
研究领域:
神经科学
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生殖生物学
·
干细胞研究
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2016
·
IF 2.937
Qin Tang,Fangling Ji,Wenhui Sun,Jingyun Wang,Jianli Guo,Lianying Guo,Yachen Li,Yongming Bao
Background 10-Hydroxy camptothecin (HCPT), a naturally occurring alkaloid, is a clinical drug for cancer chemotherapy . Baicalein (BA) is a flavonoid extracted from the root of Scutellaria baicalensis . The synergistic anti-cancer effect of BA and HCPT has not been reported. Purpose To explore whether and how BA enhances the anti-cancer effect of HCPT in BGC823 cells. Methods Cell viability was measured by MTT assay. Apoptosis and cell cycle were analyzed through flow cytometry and western blotting analysis. DNA damage was determined by a comet assay . The activity of topoisomerase I (Topo I) was detected by the plasmid DNA relaxation assay. The synergistic anti-cancer effect of BA and HCPT in vivo was tested by BGC823 xenografted tumor model. Results BA at non-toxic doses prominently enhanced the anti-cancer activities of HCPT in BGC823, MCF7 and SMMC7721 cells. Combination treatment of BA and HCPT induced BGC823 cells apoptosis mainly via intrinsic rather than extrinsic pathways, and preferentially arresting cell cycle in G1 and G2 phases with the aid of p21 . Of note, p53, the upstream regulator of cell apoptosis and cycle, was increased by 5 folds in combination group. It helped to further trigger DNA damage and inhibit Topo I catalytic activity after combination treatment of BA and HCPT. Moreover, the BGC823 xenografted tumor growth rate in nude mice was repressed in a greater degree ( P < 0.01) in the combinational group than the single-drug group. Conclusion HCPT and BA, a new and effective combination therapy, synergistically target Topo I and up-regulate p53 to induce cell apoptosis and cell cycle arrest.
研究领域:
肿瘤学
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药理学
·
分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICAL RESEARCH
·
2023
·Vol. 28
·期 1
·1-10
·
IF 4.2
Gu Shanshan,Lin Chen,Li Yanguo,Wei Zhengyu,cao Bing,Shen Zhisen,Deng Hongxia
Head and neck squamous carcinoma (HNSCC) is the seventh most common cancer worldwide. Targeted therapeutic drugs for HNSCC are still being explored. Among them, (S)-10-hydroxycamptothecin (10-HCPT), a specific inhibitor of TOP1, functions by DNA double-strand breaks that can inhibit DNA replication and trigger apoptotic cell death subsequently. Previous studies have reported that MLN4924 exerts potent anti-tumor effects by inhibiting cullin–RING ligases and causing substrate accumulation in a variety of cancers. Here, we show that MLN4924 effectively causes dose-dependent accumulation of topoisomerase I (TOP1) and blocks TOP1 ubiquitination. Importantly, neddylation inhibition with MLN4924 acts synergistically with 10-HCPT to suppress cell growth, migration and apoptosis in HNSCC cells. Mechanistically, transcriptome sequencing shows that the cytotoxic effects of the combination of MLN4924 and 10-HCPT may involve activation of the NFKB1 pathway. Taken together, our results suggest that combined treatment with MLN4924 and 10-HCPT may be an effective strategy in HNSCC.
研究领域:
肿瘤学
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分子生物学
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癌症治疗
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产品关联
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2020
·Vol. 37
·期 8
·2000164
·
IF 3.099
Liwei Su,Linhui Liu,Jinpei Hei,Huan Chen,Lianbang Wang,Yuanhao Wang,Manman Ren
V 2 O 5 , as a lithium-free cathode material, has inherent defects such as sluggish kinetics and volume change and, at the same time, requires a lithium metal anode that tends to form dendrites in liquid electrolytes. Both the lithium dendrite and the flammable electrolyte solvent bring longtime safety issues. This work introduces nonflammable inorganic–organic composite solid electrolyte to inhibit the growth of the lithium dendrite and suppress the instability caused by V 2 O 5 nanometerization. However, the long-term cycling and rate performances are still insufficient even when reducing V 2 O 5 size to about 50 nm. As an improvement, sub-10 nm V 2 O 5 /C nanosheets are designed and prepared using corn stalks as precursors through simple impregnation and calcination process. The V 2 O 5 /C offers a much better electrode/electrolyte contact and interface stability than bulk V 2 O 5 and commercial V 2 O 5 in the inorganic–organic composite solid electrolyte. The discharge capacity is 228 mAh g −1 at 0.1 C after 50 cycles and ≈110 mAh g −1 at 2.0 C.
研究领域:
材料科学
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电化学
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纳米能源
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固态电池
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电极材料设计
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Mengxing Cui,Qianmei He,Ziwei Wang,Yongjiang Yu,Huan Gao,Ziqi Liu,Honghao Peng,Han Wang,Xue Zhang,Daochuan Li,Liping Chen,Xiumei Xing,Yongmei Xiao,Wen Chen,Qing Wang
Micro/nano-plastics (MPs/NPs) are a newly discovered environmental pollutant that can be ingested by humans through food and drinking water. In this study we evaluated the impact of MPs/NPs on the intestinal barrier and its mechanism. Doses of MPs/NPs were used to treat Caco-2/HT29-MTX in-vitro model and in-vivo model. In in-vitro model, 20 nm polystyrene nanoplastics (PS-NPs) had higher cytotoxicity than larger particles (200 nm and 2000 nm), and led to the increase of the permeability along with the decreased expression of tight junction proteins. Intriguingly, 20 nm PS-NPs elevated the expression of MUC2 simultaneously. Further studies revealed that PS-NPs increased the expression of HO1 through ROS generation, and then activated p38 to elevate IL-10 secretion in Caco-2 cell. The IL-10 secreted by Caco-2 cell promoted the expression of MUC2 in HT29-MTX cell through STAT1/3. Elevated MUC2 expression alleviates the cytotoxicity of PS-NPs. Besides, increased intestinal permeability and up-regulation of MUC2 through Ho1/p38/IL-10 pathway was also observed in 20 nm PS-NPs treated mouse model. In conclusion, PS-NPs can induce the intestinal toxicity and result in the increased adaptive expression of MUC2 to resist this adverse effect. People with inadequate mucin expression need to pay more attention to the toxicity of PS-NPs. This study provided a valuable insight for clarifying the mechanism and potential risk of intestinal toxicity induced by nanoplastics.
研究领域:
环境科学
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毒理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Materials Research Express
·
2022
·Vol. 9
·期 2
·025309
·
IF 2.025
Jingdan Zhang,Yongqing Zhai
To improve the application performance of organic montmorillonite (OMt) and meet the OMt performance requirements of different fields, herein, we developed a synergistic modification method to prepare cationic–nonionic OMt (CN-Mt) using the emulsifier OP-10 and hexadecyltrimethylammonium chloride (CTAC) as comodifiers. The structure, morphology and properties of CN-Mt, cationic-OMt (C-Mt) and nonionic OMt (N-Mt) were studied by XRD, SEM, DSC, and contact angle and swell index measurements. CN-Mt has a larger layer spacing and thinner lamellae, which are obviously different from those of C-Mt and N-Mt. Moreover, CN-Mt was hydrophobic due to its denser organic surface molecules. The interlayer spacing of CN-Mt is positively related to the amount of modifier, and the amount of OP-10 contributes more to the interlayer spacing than CTAC. When the amounts of OP-10 and CTAC are 1.3 and 0.8 times the Mt cation exchange capacity (CEC), the interlayer spacing of the optimal CN-Mt is 4.49 nm, which is significantly higher than that of C-Mt (4.11 nm) at a 2.9 CEC, N-Mt (4.26 nm) at a 1.7 CEC and CN-Mt (4.20 nm) at a 0.9OP-10 + 1.5CTAC CEC. Benefitting from its structural merits, CN-Mt shows a higher degree of lamella separation, peeling and dispersion than C-Mt and N-Mt, as well as good thermal stability. Moreover, the lipophilicity and expansion of CN-Mt in medium- and low-polarity media are better than those of single-component OMt. Therefore, the structure and properties of CN-Mt can be controlled by adjusting the types and amount of modifier, further meeting the performance requirements of various application fields.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Qianqian Lan,Chao Feng,Kaiqin Ou,Zicheng Wang,Yong Wang,Tianxi Liu
Membranes with sub-10-nm pores are receiving growing interest in recognizing and separating nanocolloids , biomolecules, and soluble salts from water, but suffer from difficulty of optimizing pore structures and inferior stability. Herein, we report an efficient method for the preparation of robust nanoporous phenolic membranes with widely tunable pore sizes in the range of nanofiltration and tight-ultrafiltration. Solutions of resol precursors and poly(ethylene glycol)s (PEGs) are spin-coated to form thin films and then heated to thermopolymerize resols into phenolics. Subsequently, the films are treated in H 2 SO 4 solution to degrade PEGs, producing nanoporous phenolic membranes. Thus-prepared phenolic membranes exhibit adjustable effective pore sizes and tunable separation performances from nanofiltration to tight-ultrafiltration, which is otherwise inaccessible. Due to the thin thicknesses and rich water channels, the membranes exhibit higher permeances than other tight-ultrafiltration membranes and commercial ultrafiltration membranes with similar rejections. Furthermore, thanks to the robust frameworks and good chemical resistance of phenolics, the membranes deliver stable performances in long-term operations, elevated pressures, and acidic conditions. This work provides a facile and universal strategy for the synthesis of membranes with sub-10-nm pores for nanofiltration and tight-ultrafiltration.
研究领域:
材料科学
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环境工程
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膜技术
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2015
·Vol. 132
·期 18
·
IF 1.768
Yan-Ling Luo,Jun Zhang,Fang-Jie Han,Feng Xu,Ya-Shao Chen,Ru Liu
Thermoresponsive poly( N -isopropyl acrylamide) (PNIPAM)- block -hydroxy-terminated polybutadine- block -PNIPAM triblock copolymers were synthesized by atom transfer radical polymerization; this was followed by the in situ epoxidation reaction of peracetic acid. The copolymers were characterized by 1 H-NMR, Fourier transform infrared spectroscopy, and size exclusion chromatography measurements, and their physicochemical properties in aqueous solution were investigated by surface tension measurement, fluorescent spectrometry, ultraviolet–visible transmittance, transmission electron microscopy observations, dynamic light scattering, and so on. The experimental results indicate that the epoxidized copolymer micelle aggregates retained a spherical core–shell micelle structure similar to the control sample. However, they possessed a decreased critical aggregate concentration (CAC), increased hydrodynamic diameters, and a high aggregation number and cloud point because of the incorporation of epoxy groups and so on. In particular, the epoxidized copolymer micelles assumed an improved loading capacity and entrapment efficiency of the drug, a preferable drug-release profiles without an initial burst release, and a low cytotoxicity. Therefore, they were more suitable for the loading and delivery of the hydrophobic drug as a controlled release drug carrier. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41877.
研究领域:
材料科学
·
药学
✓ 已验证
DOI
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2014
·
IF 2.637
Zhengjun Cheng,Lei Zhang,Xiao Guo,Xiaohui Jiang,Rong Liu
Removals of Lissamine rhodamine B (LRB) and acid orange 10 (AO10) using activated carbon/surfactant from aqueous solution were optimized. Box–Behnken design (BBD) has been employed to investigate the effects of different process variables (such as time of adsorption process, temperature, pH, and initial concentration of the dye) and analyze their interactive effects in the adsorption capacity for the two dye systems. The experimental data could be simulated accurately using second order polynomial regression models because they have high correlation coefficient values ( R 2 ) of 0.9920 and 0.9938, low p -value of 0.000, and their F values of 105.96 and 137.01 for the LRB and AO10 systems, respectively. The optimum conditions were found to be t = 120 min, T = 20 °C, pH = 11.0, and C LRB = 50 mg/L for the LRB adsorption, and t = 120 min, T = 20 °C, pH = 2.5, and C AO10 = 90 mg/L for the AO10 removal. The measured maximum experimental adsorption capacities for the LRB and AO10 systems under above optimum conditions were 218.08 mg/g and 405.15 mg/g, which agreed with their corresponding predicted values (229.43 and 400.51 mg/g) well due to small relative errors of 4.95% and −1.16%, respectively. The adsorption equilibrium and kinetics of the two dye adsorptions onto AC/DDAC were also investigated. The results indicated that Langmuir model presented the best fit and the pseudo-second-order model showed excellent fit to the kinetic data.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Na Yang,Xingyuan Zhang,Juanqiong Ma,Liqing Ouyang,Zhiyong Xie,Pei Li,Xiao Lin,Qiongfeng Liao,Yanlong Chen
Paclitaxel is one of the most effective anticancer drugs ever developed and remains in high demand. Currently, most commercially available paclitaxel is produced via semi-synthesis, with key precursors like 10-deacetylbaccatin III (10-DAB) extracted from branches and leaves of yew trees. However, its low natural abundance (approximately 0.1%), the complexity of the extraction process, and the low purity of the final product present significant challenges. To address these challenges, we developed a three-dimensional covalent organic framework-based molecularly imprinted polymer (3D COF-MIP) as a novel adsorbent for the selective extraction of 10-DAB. The 3D COF-MIP demonstrated rapid adsorption kinetics (equilibrium time less than 60 min), high selectivity (imprinting factor, 6.7), and exceptional adsorption capacity. Notably, the material retained 93.5% of its initial adsorption capacity after five consecutive adsorption-desorption cycles. The developed method was successfully applied for the selective extraction and determination of 10-DAB in Taxus baccata leaves from different regions with 84.3% purity. The result demonstrated the possibility of direct extraction of 10-DAB from Taxus baccata leaves by 3D COF-MIP. This work provides a new strategy for employing COFs-MIPs to selectively extract pharmacologically active compounds from medicinal plants.
研究领域:
药用植物学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2015
·
IF 3.725
Kunjing Li,Yinfeng Li,Lu Wang,Lingxi Yang,Baoxian Ye
The electrochemical properties of 10-Hydroxycamptothecin were investigated systematically at a reduced graphene oxide modified glassy carbon electrode. A new pair of redox peaks of 10-Hydroxycamptothecin was reported for the first time with quasi-reversible process driven by adsorption. The dynamics parameters of electrode process were calculated using various electrochemical techniques. Owing to the large surface area, good conductivity of electrochemically reduced graphene oxide, the sensor exhibited sensitive activity for 10-Hydroxycamptothecin, displaying a wide linear response from 1.5 × 10 −8 mol L −1 to 5.0 × 10 −7 mol L −1 and 5.0 × 10 −7 mol L −1 to 3.0 × 10 −6 mol L −1 ( Fig. 8 ). A simple, sensitive and new voltammetric method for the determination of 10-Hydroxycamptothecin was proposed with a determination limit of 1.5 × 10 −8 mol L −1 and recoveries were determined in the range of 94.2–104%.
研究领域:
分析化学
·
药物分析
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2020
·
IF 3.233
Gulgina Mamtmin,Nuerguli Kari,Renagul Abdurahman,Patima Nizamidin,Abliz Yimit
Optical waveguide (OWG) sensors are widely used in gas detection, and sensitive materials are the key factors affecting sensor performance. Herein, we report on a 5,10,15,20-tetrakis-(4-methoxyphenyl) porphyrin (TMP) deposited optical wave guide (OWG) sensor. The porphyrin solutions with different acidity were deposited on a K + -ion exchanged glass OWG surface by spin coating, to fabricate porphyrin film-coated OWG gas sensing devices. The effects of pH values of the porphyrin solution used to prepare the sensitive film on the sensing properties of these devices were investigated. The gas sensitive performances of these devices in response to analytes were compared. Among them, the sensing devices fabricated using original and acidic solutions of porphyrin exhibited significant responses to sulfide gases and ethanediamine, respectively. The sensing device prepared using an acidic solution has a good response to ethanediamine gas (response and recovery times of the sensor for 1 ppm of EDA (ΔI = 48) are 13 and 38 s, respectively). The RSD of the output light intensity is ±2.84, the signal to noise ratio (S/N) is 5.6. The device prepared using the original solution has an excellent response to sulfide gases (H 2 S, SO 2 ), and can detect low concentration levels (ppm) of sulfide gases. These sensing devices thus have the potential to be used in food quality testing and environmental monitoring.
研究领域:
材料科学
·
化学传感器
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）