FOOD CHEMISTRY
·
2021
·
IF 7.514
Xiao Wang,Yuze Li,Yuming Jiang,Lingwei Meng,Zongxiu Nie
The analysis of free fatty acids (FFAs) in edible oils, especially their fine structure, can provide information for nutritional value evaluation and authentication. Here, a strategy based on epoxidation reaction by mCPBA combined with tandem MS was developed to identify and relatively quantify FFAs, including C C location isomers, which can rapidly distinguish different edible oils. Notably, low-abundant FFAs can be detected directly in the presence of high-abundant triacylglycerol (TAG) without complicated pretreatment. We identified a series of C C location isomers via mCPBA-nanoESI-MS/MS, among them, FA 24:1 (Δ13) and FA 24:1 (Δ17) were first identified in edible oils, and the predominant UFAs was FA 18:1 (Δ9), which occupies 98.35% of FA 18:1 in peanut oil while 89.68% in rapeseed oil. The results demonstrated that the proposed method could provide further in-depth C C positional information of oils, promoting the development of structural determination of fatty acids in food chemistry.
研究领域:
食品化学
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分析化学
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质谱分析
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Jialiang Pan,Yichun Huang,Li Liu,Yuling Hu,Gongke Li
We have developed a novel ultrasensitive online analytical system which integrated solid-phase-based extraction (SPBE) techniques with ultra-high performance liquid chromatography (UPLC) based on a fractionized sampling and stacking (FSS) strategy. FSS was proposed as a novel peak compression strategy to prevent band broadening and distortion caused by excessive solvents with high elution strength, which has been a main obstacle to conjunction of sample preparation techniques with UPLC. Such a strategy was based on online dividing a sample solution into fractions by plugs of weak mobile phase, followed by head-column stacking process, aiming to obtain a quite sharp sample zone. FSS enables UPLC to tolerate much larger injection volume of solvents with high elution strength, which facilitates hyphenation of SPBE with UPLC without peak distortion or loss of sensitivity. On the basis above, an online SPBE-UPLC system was realized by FSS, and its applicability was preliminarily verified by the successful development of a sensitive solid phase extraction-FSS-UPLC method for the determination of triazines. Subsequently an integrated online system incorporating molecularly imprinted in-tube solid phase microextraction, derivatization and FSS-UPLC was developed for the analysis of ultra trace 24-epibrassinolide. The developed method was ultrasensitive with detection limit as low as 0.7 ng/L, and the linear range of the method was 3–5000 ng/L. The endogenous 24-epibrassinolide in pollen, flower and seed samples was determined, which showed satisfactory recoveries in the range of 81.2–116% and good precision with relative standard deviation (RSD) values between 4.7 and 9.7%. This online analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex samples.
研究领域:
分析化学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2021
·Vol. 25
·期 8
·3744-3753
·
IF 5.31
Zhong-Sheng Xiao,Lei Zhao,Xiao-Ning Zhang,Han-Xian Li,Zhi-Hui Yin
It has been reported that rs67085638 in long non-coding RNAs (lncRNA)-CCAT1 was associated with the risk of tumorigenesis. Also, CCAT1 could affect chemoresistance of cancer cells to paclitaxel (PTX) via regulating miR-24-3p and FSCN1 expression. In this study, we aimed to investigate the effect of rs67085638 on the expression of CCAT1/miR-24-3p/FSCN1 and the response of colon cancer to the treatment of PTX. 48 colon cancer patients were recruited and grouped by their genotypes of rs67085638 polymorphism as a CC group (N = 28) and a CT group (N = 20). PCR analysis, IHC assay and Western blot, TUNEL assay and flow cytometry were conducted. LncRNA-CCAT1 and FSCN1 mRNA/protein were overexpressed, whereas miR-24-3p was down-regulated in the CT-genotyped patients and cells compared with those in the CC-genotyped patients and cells. The survival of colon cancer cells was decreased, whereas the apoptosis of colon cancer cells was increased by PTX treatment in a dose-dependent manner. MiR-24-3p was validated to target lncRNA-CCAT1 and FSCN1 mRNA, and the overexpression of CCAT1 could reduce the expression of miR-24-3p although elevating the expression of FSCN1. Knockdown of lncRNA-CCAT1 partly reversed the suppressed growth of CT-genotyped tumours. And the knockdown of lncRNA-CCAT1 partly reversed the dysregulation of lncRNA-CCAT1 and FSCN1 mRNA/protein in rs67085638-CT + NC shRNA mice. The findings of this study demonstrated that the presence of the minor allele of rs67085638 increased the expression of CCAT1 and accordingly enhanced the resistance to PTX. Down-regulation of CCAT1 significantly re-stored the sensitivity to PTX of colon cancer cells.
研究领域:
癌症生物学
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分子生物学
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遗传学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 8
·5366-5375
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IF 3.612
Jing Lv,Xinping Lin,Wenqing Wang,Wenhuan Xu,Caichan Li,Chaofan Ji,Huipeng Liang,Shengjie Li,Sufang Zhang,Beiwei Zhu
Summary Suanzhayu is a traditional Chinese fermented fish that often faces a long fermentation time (about 1 month) and unstable quality. This study evaluated the combined effects of papain and Lactiplantibacillus plantarum 1-24-LJ, a selected starter culture, on the quality of Suanzhayu . The addition of L. plantarum and/or papain increased the content of alcohols, esters, ketones and umami amino acids (UAAs), but decreased the content of bitter amino acids (BAAs). Besides, Lactobacillus might play a role in reducing harmful bacteria and BAAs content and increasing UAAs content, while Lactococcus and Weissella contributed to the characteristic flavour of Suanzhayu . Overall, no significant improvement in sensory quality was observed with the addition of papain alone, while better quality and faster fermentation processes were obtained with the addition of L. plantarum 1-24-LJ alone or in combination with papain. The results contribute to quality control in Suanzhayu production and the selection of starter cultures.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 12
·8303-8312
·
IF 3.9
Na Li,Mengnan Liang,Hao Zhang,Zhongxia Hua,Ling Ma,Yanyu Qi,Ke Wang
The excessive use of quinolones (QNs) has seriously threatened human health. In this study, a novel functionalized magnetic covalent organic framework Fe3O4@SiO2@Ah-COF was fabricated with biphenyl-3,3′,5,5′-tetracarbaldehyde and hydrazine hydrate (85%) as monomers and was used as a magnetic solid-phase extraction (MSPE) absorbent for the determination of 24 QNs in water and egg samples through ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The extraction parameters of MSPE were optimized, including pH, adsorbent dosage, adsorption time, and eluent type. An effective and rapid detection method was then established, which showed good linearity (R2 ≥ 0.9990), low limits of detection (0.003–0.036 μg L−1) and low limits of quantitation (0.008–0.110 μg L−1) for QNs. The good recoveries of 24 QNs in water and egg samples were in the range of 70.3–106.1% and 70.4–119.7%, respectively, with relative standard deviations lower than 10% (n = 5). As a result, Fe3O4@SiO2@Ah-COF is a promising magnetic adsorbent, and the established method was successfully applied for the determination of 24 QNs in water and egg samples.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
Animals
·
2026
·Vol. 16
·期 5
·749
·
IF 3.2
Xitong Guan,Haokai Ma,Rui Liu,Yiou Xu,Diene Gnagna,Xiujie Yin,Yonggen Zhang,Yang Li
Microplastic pollution is rising worldwide and may harm both animals and people. Dairy cows rely on a large stomach compartment, called the rumen, to break down feed with the help of many microbes, and this process produces gases and acids that reflect healthy digestion. In this study, we tested whether tiny plastic particles made of polystyrene, in different sizes and amounts, change rumen digestion and whether rumen microbes can break these plastics down. We found that exposure to these particles reduced fermentation activity, shown by lower gas production and lower levels of volatile fatty acids, and the negative effects became stronger as the amount of microplastics increased. At the same time, higher amounts and larger particles increased the activity of some digestive enzymes, suggesting a stress response in the microbial system. Microplastics also lowered the abundance of certain microbes and changed the chemicals produced during digestion. After 24 h, the amount of microplastic particles decreased, indicating that rumen microbes can partially degrade them and generate small chemical products that could further disturb rumen balance. These findings help explain how microplastics may influence ruminant health and suggest a possible biological route to reduce microplastic contamination. Microplastic pollution is increasingly serious worldwide, threatening human and animal health. The cow rumen is a key organ for nutrient digestion and absorption, and its fermentation is closely related to rumen microorganisms. Here, we investigated how polystyrene microplastics (PS-MPs) with varying particle sizes and concentrations affect rumen fermentation and the biodegradability of PS-MPs by rumen fermentation. The results reveal that exposure to PS-MPs lowered gas production and gas concentrations, as well as volatile fatty acid content, and these decreases were positively correlated with PS-MP concentration. However, higher PS-MP concentration and larger particle size increased the activity of carboxymethyl cellulose, β-glucosidase, and xylanase. Furthermore, PS-MP exposure reduced the abundance of certain rumen microorganisms and altered metabolic pathways and metabolites linked to PS-MP biodegradation. It was also found that PS-MP content decreased significantly after 24 h fermentation. Therefore, PS-MPs can inhibit rumen fermentation by affecting the rumen microbiome, and rumen microorganisms and their secreted enzymes can biodegrade PS-MPs to produce styrene and derivatives; such small molecules may further disrupt rumen homeostasis, thereby affecting lactation performance. In addition, rumen microbial degradation of PS-MPs provides a new idea to resolve future microplastic contamination challenges.
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DOI
PubMed
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 10
·880
·
IF 3.7
Siqi Zheng,Mingyue Fang,Jiaxin Huang,Yanbin Li,Yuxia Mei
Antrodia cinnamomea(AC), a rare fungus endemic to Taiwan, contains high levels of various secondary metabolites, notably triterpenoids, having useful medicinal and pharmacological properties. Techniques for increasing the production of AC triterpenoids (ACT) for medicinal purposes are a high research priority. We measured and compared the biomass and ACT content of AC mycelia under various liquid fermentation culture conditions. Relative gene expression levels of ten enzymes involved in the mevalonate (MVA) pathway and “subsequent group modification pathway” were determined, and correlation analysis was performed to evaluate the roles of these enzyme genes in ACT synthesis. Two representative genes encoding the enzymes lanosterol synthase (AcLSS) and sterol C-24 reductase (AcERG4), whose activity is closely associated with ACT content, were selected for homologous expression.AcLSSandAcERG4were separately linked to plasmid pCT74, and transformed into prepared AC protoplasts to obtain two recombinant strains, termed RpLSS and RpERG4, by polyethylene glycol (PEG)-CaCl2-mediated protoplast transformation. Upregulated expression levels ofAcLSSandAcERG4(1.78- and 1.41-fold, respectively) were associated with significantly higher (1.82- and 1.37-fold, respectively) ACT content in the recombinant strains in comparison with the wild-type. Our findings provide a theoretical and practical basis for the enhancement of ACT production using homologous expression techniques.Keywords:Antrodia cinnamomea;triterpenoids;homologous expression;synthetic pathway
研究领域:
真菌学
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生物技术
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药理学
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分子生物学
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DOI
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-7
·
IF 16.6
Wang Zhi-Da,Liang Song,Yang Yuqing,Liu Zhen-Ning,Duan Xiao-Zheng,Li Xinpei,Liu Tianbo,Zang Hong-Ying
Inorganic salts usually demonstrate simple phasal behaviors in dilute aqueous solution mainly involving soluble (homogeneous) and insoluble (macrophase separation) scenarios. Herein, we report the discovery of complex phase behavior involving multiple phase transitions of clear solution – macrophase separation – gelation – solution – macrophase separation in the dilute aqueous solutions of a structurally well-defined molecular cluster [Mo 7 O 24 ] 6− macroanions with the continuous addition of Fe 3+ . No chemical reaction was involved. The transitions are closely related to the strong electrostatic interaction between [Mo 7 O 24 ] 6− and their Fe 3+ counterions, the counterion-mediated attraction and the consequent charge inversion, leading to the formation of linear/branched supramolecular structures, as confirmed by experimental results and molecular dynamics simulations. The rich phase behavior demonstrated by the inorganic cluster [Mo 7 O 24 ] 6− expands our understanding of nanoscale ions in solution.
研究领域:
无机化学
·
材料科学
·
物理化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Yongmei Wu,Kai Sheng,Yongqin Liu,Qian Yu,Baoxian Ye
In this work, an ultrasensitive electrochemical microRNA detection strategy was developed based on porous palladium-modified horseradish peroxidase sphere ( [email protected] ) and target-induced assembly of DNAzyme. A highly loaded HRP sphere was prepared by covalent layer-by-layer assembly with CaCO 3 as sacrificial template for the first time, and was further modified with porous Pd. Notably, [email protected] composite showed a good redox activity of HRP and electrocatalytic activity toward H 2 O 2 . The utilization of [email protected] as electrochemical signal indicator and enhancer to fabricate biosensor could avoid the need for additional redox mediator and amplify the detection sensitivity. Moreover, target recycling amplification was achieved by Pb 2+ -induced cleavage of ternary “Y” structure, circumventing the use of labile nuclease . Subsequent DNA concatamer synthesized through rolling circle amplification (RCA) reaction with cleaved hairpin probe as primer, hybridized with plentiful [email protected] probes, which led to the increased loading of redox-active and electrocatalytic [email protected] for sensitivity improvement. So the proposed electrochemical biosensor detected miRNA-24 down to 0.2 fM (S/N = 3) with a wide linear range from 3 fM to 1 nM. With bifunctional [email protected] tag, DNAzyme-aided target recycle and programmable junction probe, this strategy possessed the advantages of high efficiency, high sensitivity, low cost and versatility, and thus held great promise for other low-abundance nucleic acids determination.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
·
2025
·
IF 2.9
Sili Chen,Liqin Xiong,Xinlan Wu,Lu Chen,Junwei Zhang,Guanqing Jiang,Xin Li,Qiaoyuan Yang
We investigated the toxicity of benzo[ghi]perylene (BghiP), a prevalent polycyclic aromatic hydrocarbon (PAH). Transcriptome analysis of control and BghiP-exposed human bronchial epithelial cells (BEAS-2B) led to the identification of a novel chimeric RNA (chRNA), TVP23C-CDRT4, whose expression is significantly downregulated by BghiP in vitro and in vivo. Funcionally, overexpression of TVP23C-CDRT4 inhibited BghiP-induced cell proliferation and DNA damage, whereas its knockdown exacerbated these detrimental effects. Mechanistically, TVP23C-CDRT4 acts as a molecular decoy for miR-24-3p. Thus, BghiP-induced suppression of TVP23C-CDRT4 unveils a novel mechanism of PAH toxicity, highlighting its anti-proliferative and genome-protective roles relevant to the early stages of carcinogenesis.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 13
·2161-2176
·
IF 3.614
Yan-lin Zhao,Lin-lin Zhao,Yu-xin you,Xiao-xiao Zheng,Yan Du,Dao-quan Tang
We aim to establish a simple and easy high-performance liquid chromatography system coupled with an ultraviolet detector suitable for simultaneous determination of 24 antiepileptic drugs in human plasma. Optimized chromatographic separation was performed on a ZORBAX Eclipse Plus-C18 (4.6 × 150 mm 2 , 3.5 μm) column with acetonitrile and 5 mM potassium dihydrogen phosphate water solution as mobile phase. Note that, 24 antiepileptic drugs were divided into three groups and eluted with different gradient procedures, respectively. The column temperature was maintained at 35°C and the detection wavelength was set at 210 nm. Plasma was processed with ethyl acetate or acetonitrile. The calibration curves of 24 antiepileptic drugs demonstrated good linearity within the test range ( r > 0.996). The intra- and inter-batch precision and accuracy were all less than 15%, while extraction recoveries were in the range of 74.57–90.89% with the relative standard deviation values less than 15%. The validated methods have been successfully applied to determination of some antiepileptic drugs in rat or patient plasma. Those results indicated that the developed methods were simple and easy, and could be suitable for the determination of 24 antiepileptic drugs in plasma just by changing the gradient elution procedures of mobile phase.
研究领域:
分析化学
·
药理学
·
临床化学
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DOI
高影响因子
ADVANCED MATERIALS
·
2026
·
IF 29.1
Longqing Chen,Youtan Pan,Yuwei Zhao,Meijing Xiao,Yusha Gao,Zhuoran Lv,Shuai Li,Hao Nie,Hao Yang,Fuqiang Huang,Wujie Dong
Sodium-ion batteries are naturally suitable for large-scale energy storage due to the low cost, yet the high-performance anode material remains a key challenge. Alloy-type bismuth (Bi)-based anodes have high theoretical capacity and an appropriate operating voltage plateau, but the main obstacles are cycle life, rate, and wide operating temperature. This work proposes an innovative design of Bi-based anode materials, where Bi serves as the sodium storage unit and is integrated with a robust Ti-O framework of excellent Na + diffusion ability. Different compounds and synthesis/modified methods are systematically investigated to optimize crystal structure, size, morphology, and conductivity. The optimal Bi 4 Ti 3 O 12 −x delivers a reversible capacity of 330 mAh g −1 at 0.5 C, 132 mAh g −1 at 150 C, and 70 mAh g −1 even at 250 C. Besides, it can operate under an ultra-wide temperature range from −70°C to 80°C. A reversible charge/discharge capacity of 151 mAh g −1 at −55°C is achieved. The capacity retention is 97% (25°C, 20 C) and 61% (−40°C, 5 C) over 24 000 cycles. The proposed design of incorporating alloy materials as sodium storage units into the stable host framework unit is expected to complement the effective strategy of practical low-temperature fast-charging electrodes.
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高影响因子
危险品化学品经营许可证（带存储）