JOURNAL OF APPLIED MICROBIOLOGY
·
2022
·Vol. 133
·期 5
·3161-3175
·
IF 4.059
Pan Deng,Wang Xing‐Yun,Zhou Jin‐Wei,Yang Liang,Khan Abbas,Wei Dong‐Qing,Li Jun‐Jian,Jia Ai‐Qun
AimsIn the current study the anti‐virulence and anti‐biofilm activities of the cinnamic acid derivative, 3‐methoxycinnamic acid, was investigated against Agrobacterium tumefaciens.Methods and ResultsBased on the disc diffusion test and β‐galactosidase activity assay, 3‐methoxycinnamic acid was shown to interfere with the quorum sensing (QS) system of A. tumefaciens. Crystal violet staining assay, phenol‐sulfuric acid method, Bradford protein assay and confocal laser scanning microscopy (CLSM) revealed that the biofilm formation of A. tumefaciens was inhibited after the treatment of 3‐methoxycinnamic acid. Employing high‐performance liquid chromatography (HPLC) analysis of culture supernatant revealed that the production of 3‐oxo‐octanoylhomoserine lactone (3‐oxo‐C8‐HSL) decreased concentration‐dependently after treatment with 3‐methoxycinnamic acid. Swimming and chemotaxis assays also indicated that 3‐methoxycinnamic acid had a good effect on reducing the motility and chemotaxis of A. tumefaciens. In addition, the RT‐qPCR, molecular docking and simulations further demonstrated that 3‐methoxycinnamic acid could competitively inhibit the binding of 3‐oxo‐C8‐HSL to TraR and down‐regulate virulence‐related genes.Conclusions3‐Methoxycinnamic acid is proved to have good anti‐virulence and anti‐biofilm activities against A. tumefaciens.Significance and Impact of the StudyThis is the first study that investigates the anti‐virulence and anti‐biofilm activities of 3‐methoxycinnamic acid against A. tumefaciens. With its potential QS‐related virulence and biofilm inhibitory activities, 3‐methoxycinnamic acid is expected to be developed as a potent pesticide or adjuvant for the prevention and treatment of crown gall caused by A. tumefaciens.
研究领域:
微生物学
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植物病理学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemPlusChem
·
2020
·Vol. 85
·期 1
·240-246
·
IF 2.753
Sheng Tang,Xinyu Xiao,Jing Hu,Bo Gao,Hunglin Chen,Zongyang Peng,Jianchun Wen,Masanao Era,Dechun Zou
Graphical Solvent-free is best : Mixed halide hybrid perovskites (MHPs) are prepared through a solvent-free mechanochemical synthesis (MCS) route with superior control over halide compositions. The MCS route eliminates the problem in the preparation of MAPb(I x Br 1− x ) 3 with continuously varying x , while maintaining the material properties and suppressing phase segregation in solvent synthesis (SS) routes. Thus, the optical band gaps can be tuned easily by compositional control. Hybrid perovskites have recently received much attention in optoelectronic applications. However, hybrid perovskites are unstable in a humid environment. Mixed halide perovskites (MHPs) show enhanced stability and band-gap tunability upon engineering of their halide composition. Here, MHPs are prepared through a solvent-free mechanochemical synthesis (MCS) route that allows superior control over halide compositions than the solvent synthesis routes (SS). The MCS route eliminates the problem in the preparation of MAPb(I x Br 1− x ) 3 with continuously varying x , while maintaining the material properties and suppressing phase segregation present in SS routes. UV-vis absorption and X-ray diffraction patterns confirm the production of the desired pure-phase MHPs. For MAPb(I x Br 1− x ) 3 (0≤ x ≤1), with increased ratio of halide ( x ), the cubic phase gradually transforms into the tetragonal phase and band-gap tunability is accomplished. The MCS route for the preparation of MHPs is a very promising and efficient technique for superior control in optoelectronic properties, leading to improved control in fabrication approaches.
研究领域:
材料科学
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化学
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光电技术
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2022
·Vol. 49
·期 8
·3381-3393
·
IF 3.134
Yang Haoguo,Guo Yijun,Han Shitong,Xi Hailing,Lin Huaxiang,Yuan Rusheng,Ding Zhengxin,Long Jinlin,Lin Qun
Mustard gas is a kind of erosive poison with great harm and used as a chemical weapon since World War. It has suggested that the photocatalytic technology is an effective method to eliminate it. Because the small difference in molecular structure between 2-CEES and Mustard gas, and the less toxic of 2-CEES, 2-CEES is often used as a simulator to evaluate the photocatalytic activity of catalysts. In this work, symbiotic mixture of Bi 2 WO 6 and Bi 6 O 6 (OH) 3 (NO 3 ) 3 ·1.5H 2 O nanosheets doped with Mo (Mo-B-B) have been synthesized by a facile hydrothermal treatment. The samples were characterized by XRD, DRS, XPS, SEM, TEM, Raman, and PL. The photocatalytic activity of the samples was evaluated by measuring the degradation of 2-CEES under visible light irradiation. The results showed that the doped Mo reduce the Eg of bare symbiotic mixture and improve the separation efficiency of photo-generated carriers that is beneficial to photocatalytic activity. The optimum amount of doped Mo is 5% that exhibits the best activity for degradation for 2-CEES.
研究领域:
材料科学
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环境科学
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化学工程
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纳米技术
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光催化
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2021
·Vol. 32
·期 5
·5821-5830
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IF 2.478
Hu Peng,Deng Zhaoping,Wang Teshen,Li Zepeng,Ma Yanlin
In this study, a series of Eu 3+ -doped NaBaBi 2 (PO 4 ) 3 red phosphors were synthesized by high-temperature solid-phase reaction method, and the phase structure and photoluminescence properties of the samples were investigated in detail. All the diffraction peaks of the phosphors with various Eu 3+ concentrations are well matched with the NaBaBi 2 (PO 4 ) 3 standard phase, indicating that Eu 3+ ions have no effect on the host structure. Under the excitation of 391 nm wavelength, the emission spectra consisted of four characteristic emission peaks at 595, 617, 656 and 703 nm, respectively. The strongest emission peaking at 617 nm originates from the 5 D 0 → 7 F 2 electron transitions of Eu 3+ ions. The optimum concentration of Eu 3+ ions in NaBaBi 2 (PO 4 ) 3 :Eu 3+ is 9% concentration. The phosphor exhibits strong absorption capacity in the near-ultraviolet region. The Commission International de I’Eclairage chromaticity coordinates of the NaBaBi 2 (PO 4 ) 3 :Eu 3+ samples are located in the red region, indicating that the phosphors are suitable red component to applied in near-ultraviolet-based white light-emitting diodes.
研究领域:
材料科学
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光学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Jianxia Sun,Haixia Luo,Xinghua Li,Xusheng Li,Yeyu Lu,Weibin Bai
Pyranoanthocyanins are anthocyanin derivatives that are formed during fermentation and oxygenation processes in aged wine. They play a critical role in assessing the aging of wine based on its colour quality. Naturally, the formation process of pyranoanthocyanins is fairly slow. In this respect, the present work studies the role and efficiency of ultrasonic treatment in accelerating the formation of methylpyranocyanidin-3- O -glucoside and investigates the spectral characteristics as well as the stability of this derivative toward heat, bisulfite bleaching and pH variation in a simulation system. Our results showed that the yield of methylpyranocyanidin-3- O -glucoside can be increased by 32.5% by ultrasonic treatment at 100 W for 40 min, and excessive treatment (≥60 min) does not have a positive effect on the yield. Moreover, this derivative exhibits a better stability toward heat, pH variation and bisulfite bleaching than its precursor cyanidin-3- O -glucoside.
研究领域:
食品科学
·
化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Shicheng Lei,Minhao Xie,Bing Hu,Li Zhou,Yi Sun,Muhammad Saeeduddin,Hongcheng Zhang,Xiaoxiong Zeng
In the present study, pear polyphenol oxidase (PPO) was purified, immobilized and applied for the synthesis of theaflavin-3,3′-digallate (TF3). Firstly, PPO of pear ( Pyrus bretschneideri Rehd cv. Huangguan) was purified 24.36-fold in specific activity with a recovery of 6.77% by sequential use of precipitation with 70% saturated ammonium sulfate and chromatography of DEAE-Sepharose Fast Flow column. The purified pear PPO, found to be a dimer of identical subunits of molecular mass 35 kDa, was then successfully immobilized onto Fe 3 O 4 /chitosan nanoparticles via glutaraldehyde coupling reaction. Finally, TF3 was effectively synthesized by applying the immobilized PPO as biocatalyst with epicatechin gallate (ECG) and epigallocatechin-3- O -gallate (EGCG) as substrates, and the maximum yield of TF3 was 42.23% based on the amount of ECG added. In addition, the immobilized enzyme still remained 85% of its initial ability after successive usage of 8 cycles and could be stored for 30 days with less loss of activity. All the results suggest that the developed procedure is quite useful and has great potential for synthesis of TF3 and other theaflavins.
研究领域:
食品科学
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酶工程
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植物学
✓ 已验证
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Yuguo Zheng,Mei Chen,Renfeng Zhang,Wei Xue
BACKGROUND Plant pathogens cause substantial crop losses annually, posing a grave threat to global food security. Fungicides have usually been used for their control, but the rapid development of pesticide resistance renders many ineffective, therefore the search for novel and efficient green pesticides to prevent and control plant diseases has become the top priority in crop planting. RESULTS The results of bioassay studies indicated that most of the target compounds showed certain antimicrobial activity in vitro . In particular, compound X7 showed high inhibitory activity against Xanthomonas oryzae pv. oryzae ( Xoo ), with an EC 50 value of 27.47 μg mL −1 , surpassing conventional control agents such as thiazole zinc (41.55 μg mL −1 ) and thiodiazole copper (53.39 μg mL −1 ). Further studies on molecular docking showed that X7 had a strong binding affinity with 2FBW. The morphological change observed by scanning electron microscopy indicated that the surface of Xoo appears wrinkled and cracked under X7 treatment and a total of 2662 proteins were identified by label-free proteomic analysis. Three experiments have elucidated the mechanism whereby X7 induced considerable changes in the physiological and biochemical properties of Xoo , which in turn affected the reproduction and growth of bacteria. CONCLUSION This work represents a pivotal advancement, offering important reference for the research and development therapeutics in combating plant pathogens. © 2024 Society of Chemical Industry.
研究领域:
农业化学
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植物病理学
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药物科学
✓ 已验证
DOI
PubMed
高影响因子
European Journal of Wood and Wood Products
·
2023
·Vol. 81
·期 4
·1011-1020
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IF 2.6
Shi Danchen,Wang Wenxuan,Liang Daxin,He Zengcheng,Wang Yonggui,Xiao Zefang,Yu Yang,Wang Haigang,Xie Yanjun
Plantation poplar is one of the most commonly used fast-growing woods in China. However, it is susceptible to weathering due to climatic conditions such as sunlight irradiation, rain, temperature changes, and oxidation. Ultraviolet (UV)-induced discoloration is a noticeable problem of this wood during its outdoor use. In this study, titanium dioxide (TiO 2 ) nanoparticles were deposited onto a wood surface via a bridge of 3-isocyanatopropyltriethoxysilane (IPTS). Accelerated weathering tests were conducted by exposing wood veneers to UV, water spraying, and condensation for 336 h to evaluate the treatment effect on their anti-weathering properties. During treatment, TiO 2 nanoparticles were bonded with IPTS and grafted onto the wood surface via the isocyanate group. The nanoparticles functioned as a UV filter and reduced photodegradation, while IPTS helped prevent the wood from collapsing. Fourier transform infrared spectroscopy showed that the photodegradation of lignin during weathering was effectively inhibited after treatment. After artificial weathering for 192 h, the color difference (Δ E ) of both untreated and IPTS-treated woods increased up to 40 while that of IPTS-TiO 2 -treated wood increased only to 12. The results suggest that IPTS-TiO 2 modification is feasible for improving the anti-weathering properties of poplar wood and may provide an effective strategy for industrial wood modification.
研究领域:
材料科学
·
林学
✓ 已验证
产品关联
DOI
高影响因子
BRAIN RESEARCH
·
2023
·
IF 2.9
Yong-Juan Gu,He-Ya Qian,Fang Zhou,Ling Zhang,Long Chen,Yu Song,Ya-Nan Chen,Hai-Long Zhang
Background Bone cancer pain (BCP) remains a clinical challenge due to the limited and side effects of therapeutic methods. Folic acid has been known as an FDA approved dietary supplement and proved to have an analgesic effect in neuropathic pain. Here we investigate the role and mechanism of folic acid in bone cancer pain of a rat model. Methods Walker 256 tumor cells were inoculated into the left tibia of rats to induce bone cancer pain model. Pain reflex were assessed by paw withdrawal threshold (PWT) response to Von Frey filaments and paw withdrawal latency (PWL) response to thermal stimulation. Folic acid was injected intraperitoneally to evaluate its analgesic effect in rats with bone cancer pain. Western blotting and qPCR were used to determine P2X2/3 receptor protein and mRNA levels in ipsilateral L4-6 dorsal root ganglion (DRG) and spinal dorsal horn (SDH). Results The PWT and PWL of rats with bone cancer pain were obviously decreased compared to the naïve and sham rats. Interestingly, continuous folic acid treatment significantly increased the PWT and PWL of rats with bone cancer pain. P2X2 and P2X3 receptors were clearly upregulated at both mRNA and protein expression in L4-6 DRG and SDH of rats with bone cancer pain. P2X2 and P2X3 receptors were mainly localized with CGRP (calcitonin gene-related peptide) or IB4 (isolectin B4) positive neurons in L4-6 DRG of rats with bone cancer pain. Notably, continuous folic acid treatment significantly reduced the expression of P2X2 and P2X3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Finally, intrathecal injection of A317491 (a selective antagonist of P2X2/3 receptors) markedly elevated the PWT and PWL of rats with bone cancer pain. Conclusion These results suggest that folic acid has an effective antinociceptive effect on bone cancer pain, which is mediated by downregulating P2X2/3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Folic acid may be a novel therapeutic strategy in cancer patients for pain relief.
研究领域:
药理学
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肿瘤学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2024
·
IF 4.5
Lixin Ding,Feng Wang,Zilei Wang,Yongxiang Pan,Teli Liu,Liansheng Cheng,Wenting Liu,Kuke Ding,Hua Zhu,Zhi Yang
Lymphocyte activation gene 3 (LAG-3) has attracted much attention as a potentially valuable immune checkpoint. Individual identification of LAG-3 expression at screening and during treatment could improve the successful implementation of anti-LAG-3 therapies. HuL13 is a human IgG1 monoclonal antibody that binds to the LAG-3 receptor in T cells. Here, we used [89Zr]Zr-labeled HuL13 to delineate LAG-3+ T-cell infiltration into tumors via positron emission tomography (PET) imaging. A549/LAG-3 cells, which stably express LAG-3, were generated by infection with lentivirus. The uptake of [89Zr]Zr-DFO-HuL13 in A549/LAG-3 cells was greater than that in the negative control (A549/NC) cells at each time point. The equilibrium dissociation constant (Kd) of [89Zr]Zr-DFO-HuL13 for the LAG-3 receptor was 8.22 nM. PET imaging revealed significant uptake in the tumor areas of A549/LAG-3 tumor-bearing mice from 24 h after injection (SUVmax = 2.43 ± 0.06 at 24 h). As a proof of concept, PET imaging of the [89Zr]Zr-DFO-HuL13 tracer was further investigated in an MC38 tumor-bearing humanized LAG-3 mouse model. PET imaging revealed that the [89Zr]Zr-DFO-HuL13 tracer specifically targets human LAG-3 expressed on tumor-infiltrating lymphocytes (TILs). In addition to the tumors, the spleen was also noticeably visible. Tumor uptake of the [89Zr]Zr-DFO-HuL13 tracer was lower than its uptake in the spleen, but high uptake in the spleen could be reduced by coinjection of unlabeled antibodies. Coinjection of unlabeled antibodies increases tracer activity in the blood pool, thereby improving tumor uptake. Dosimetry evaluation of the healthy mouse models revealed that the highest absorbed radiation dose was in the spleen, followed by the liver and heart wall. In summary, these studies demonstrate the feasibility of using the [89Zr]Zr-DFO-HuL13 tracer for the detection of LAG-3 expression on TILs. Further clinical evaluation of the [89Zr]Zr-DFO-HuL13 tracer may be of significant help in the stratification and management of patients suitable for anti-LAG-3 therapy.
研究领域:
癌症研究
·
免疫学
·
医学成像
·
核医学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Research Express
·
2021
·Vol. 8
·期 10
·105002
·
IF 1.62
Wenchang Xi,Pan Jian,Xisong Mao,Deyang Ning,Xuewen Xia,Guohua Liu,Dongyang Shi,Yuanpei Lan
In this work, aiming to evaluate and compare the luminescent property of Eu3+ doped La(OH)3 nanorods and its decomposed compounds of LaOOH and La2O3, La(OH)3: Eu3+ nanorods were synthesized and the decomposition behaviors in air and hydrogen were revealed. Then the luminescent property of 0%–8% Eu3+ doped La(OH)3 nanorods and the 300 °C–800 °C calcined La(OH)3: Eu3+- 4% were studied. Results show that LaOOH is intermediate product during the degradation of La(OH)3 in air or H2, which can be formed at a temperature range in 372.3 °C–592.9 °C in air. Intensity of emission spectra of La(OH)3: Eu3+ nanorods increases with the rising of Eu3+ dopant concentration, and the emission property of Eu3+ doped LaOOH and La2O3 is varied and strengthened with that of La(OH)3: Eu3+. Luminescent matrixes of LaOOH and La2O3 show similar emission spectrum and intensity, and the transitions of the D0 → 7F1 and D0 → 7F2 of Eu3+ each bifurcate into two peaks for both europium (III) doped La2O3 and LaOOH. The revealed results suggest that LaOOH is a relative thermal-stable compound and should be an appropriate matrix as similar as La2O3 for luminescent applications.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Daixiang Wei,Lingqi Kong,Xinshun Tan,Shiqing Zheng
The binary vapor–liquid equilibrium (VLE) data at the pressure of 101.3 kPa, which are 3,3-dimethyloxetane + methyl cyclohexane and 3-chloro-2,2-dimethyl-1-propanol + methyl cyclohexane, have been measured using a modified Rose vapor-recirculating equilibrium still. The reliability of VLE data was verified by thermodynamic consistency of area test of Herington and point test of Van Ness. The experimental data were regressed with activity coefficient models of Wilson, nonrandom two-liquid, and UNIQUAC, respectively, and the corresponding binary interaction parameters were obtained using the maximum likelihood method. The root-mean square deviations between calculated and experimental data for the vapor phase molar fraction and equilibrium temperature were less than 0.0119 and 0.58 K, which indicated that the fitted results for the three models were within acceptable limits.
研究领域:
化学工程
·
热力学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）