JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-17
·
IF 12.6
Guo Xiaoping,Zuo Xu,Zheng Wenxue,Zhao Dan,Dong Chao,Zou Zheng,Shen Yuanyuan,Xu Caina,He Chaoliang,Wang Fang
Asthma is a chronic disease with typical pathological features such as airflow limitation, airway inflammation and remodeling. Of these, neutrophilic asthma is considered to be the more severe and corticosteroid-resistant subtype of asthma. Increasing evidence suggests that patients with neutrophilic asthma often accompany with dysbiosis of the internal microbiota, where the increased abundance of non-typeable Haemophilus influenzae (NTHi) is closely related to the neutrophilic asthma phenotype. Furthermore, emerging evidence suggests that reactive oxygen species (ROS) are pivotal in the pathogenesis of neutrophilic asthma. In this study, matrix metalloproteinase-9 (MMP-9)-responsive, catalase-loaded nanogels (M-CAT-NGs) were synthesized, which was composed of MMP-9-sensitive peptide (VPMS), arginine-grafted chitosan and maleimide (CS-Arg-Mal), catalase (CAT), sodium citrate (SC) and ε-poly(L-lysine) (ε-PLL). The M-CAT-NGs showed potent antimicrobial effects and exerted excellent therapeutic effects in the presence of MMP-9 by causing VPMS rupture and responsive release of CAT. In vitro experiments revealed that M-CAT-NGs effectively inhibited the proliferation of NTHi, Staphylococcus aureus ( S. aureus ), and Escherichia coli ( E. coli ), while also demonstrating the capacity to modulate the inflammatory response induced by lipopolysaccharide (LPS) and hydrogen peroxide (H 2 O 2 ) stimulation. In vivo experiments demonstrated that nebulized inhalation of M-CAT-NGs was effective in reducing airway hyperresponsiveness (AHR), alleviating inflammation, downregulating the expression level of ROS in the lung tissues, thus enabling the effective management of neutrophilic asthma. Thus, the development of M-CAT-NGs has shown strong potential for the clinical management of neutrophilic asthma by modulating the inflammatory response.
研究领域:
医学
·
药学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Hao Meng,Jianlong Su,Qi Shen,Wenzhi Hu,Pinxue Li,Kailu Guo,Xi Liu,Kui Ma,Weicheng Zhong,Shengqiu Chen,Liqian Ma,Yaying Hao,Junli Chen,Yufeng Jiang,Linlin Li,Xiaobing Fu,Cuiping Zhang
Chronic diabetic wounds are characterized by prolonged inflammation and excessive accumulation of M1 macrophages, which impede the healing process. Therefore, resolving inflammation promptly and transitioning to the proliferative phase are critical steps for effective diabetic wound healing. Exosomes have emerged as a promising therapeutic strategy. In this study, a smart hydrogel capable of responding to pathological cues in the inflammatory microenvironment to promote the transition from inflammation to proliferation by delivering M2 macrophage-derived exosomes (M2-Exos) is developed. The smart hydrogel is synthesized through the cross–linking of oxidized dextran, a matrix metalloproteinase (MMP)-9-sensitive peptide, and carboxymethyl chitosan containing M2-Exos. In response to elevated MMP-9 concentrations in the inflammatory microenvironment, the hydrogel demonstrates diagnostic logic, adjusting the release kinetics of M2-Exos accordingly. The on-demand release of M2-Exos facilitated macrophage polarization from the M1 to the M2 phenotype, thereby promoting the transition from the inflammatory to the proliferative phase and accelerating diabetic wound healing. The transcriptomic analysis further reveals that the MMP-9-responsive hydrogel with M2-Exos delivery exerts anti-inflammatory and regenerative effects by downregulating inflammation-related pathways. This study introduces an innovative, microenvironment-responsive exosome delivery system that enables precise control of therapeutic agent release, offering a personalized approach for the treatment of chronic diabetic wounds.
研究领域:
生物医学工程
·
组织工程
·
伤口愈合
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 15
·12620-12627
·
IF 2.22
Ma Xiaomeng,Li Congyi,Fan Zijia,Wu Haitao,Zhang Yuping,Zhang Zhiliang
Low temperature-sintered ceramics Nd 2 Zr 3 (MoO 4 ) 9 were successfully prepared via reaction-sintering method. The sintering behavior, microwave dielectric properties, crystal structure, and microstructure were analyzed systematically. XRD and Rietveld refinement results revealed that all samples formed a pure trigonal structure phase with the space group of R-3c. Density determination showed that ceramics possessed the highest relative density when sintered at 800 °C for 6 h. SEM image further confirmed that ceramics possessed dense and homogeneous microstructure at the optimum sintering temperature 800 °C. Far infrared spectra analysis showed the structural phonon oscillation at infrared region played an important role in polarization. Nd 2 Zr 3 (MoO 4 ) 9 ceramics obtained the optimum microwave dielectric properties of ε r = 10.01, Q × f = 55,923 GHz, τ f = − 13.81 ppm/ °C when sintered at 800 °C for 6 h.
研究领域:
材料科学
·
陶瓷
·
微波介电材料
·
固体化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Xiaoyan Zhou,Qianqian Zheng,Changyong Wang,Jiake Xu,Jian-Ping Wu,Thomas Brett Kirk,Dong Ma,Wei Xue
The drug/gene codelivery is a promising strategy for cancer treatment. Herein, to realize the codelivery of docetaxel and MMP-9 siRNA plasmid efficiently into tumor cells, a star-shaped amphiphilic copolymer consisting of hyperbranched polyglycerol derivative (HPG-C18) and dendritic poly(l-lysine) (PLLD) was synthesized by the click reaction between azido-modified HPG-C18 and propargyl focal point PLLD. The obtained HPG-C18-PLLD could form the nanocomplexes with docetaxel and MMP-9, and the complexes showed good gene delivery ability in vitro by inducing an obvious decrease in MMP-9 protein expression in MCF-7 cells. The apoptosis assay showed that the complex could induce a more significant apoptosis to breast cancer cells than that of docetaxel or MMP-9 used alone. In vivo assay indicated that the codelivery strategy displayed a better effect on tumor inhibition. Moreover, HPG-C18-PLLD displayed lower toxicity as well as better blood compatibility compared to polyethylenimine PEI-25k, which may be the result of that HPG-C18-PLLD showed the comparative MMP-9 delivery ability in vivo compared with PEI-25k even if it showed the slight lower transfection efficiency in vitro. Therefore, HPG-C18-PLLD is a safe and effective carrier for the codelivery of drug/gene, which should be encouraged in tumor therapy.
研究领域:
药物学
·
癌症研究
·
聚合物化学
·
基因治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2017
·
IF 4.784
Liyan Yang,Yu Yan,Feilong Cai,Jinghai Li,Tao Wang
Conjugated polymer poly(9-vinylcarbazole) (PVK) has been explored as an efficient hole transport material (HTM) to replace poly(3,4-ethylenedio-xythiophene): poly(styrenesulfonate) (PEDOT:PSS) for the preparation of p-i-n type, inverted planar heterojunction perovskite solar cells. The PVK film can be facilely deposited by room-temperature solution-casting without any doping or post treatments. Using MAPbI 3 as the photoactive layer, a 15.8% stabilized power conversion efficiency was achieved in ITO/PVK/MAPbI 3 /PCBM/Ag devices, which is a significant improvement compared with 12.1% from devices using PEDOT:PSS as the HTM. The perovskite photoactive layer cast on PVK HTM has higher crystallinity and less PbI 2 residues, leading to a higher charge recombination resistance of the device. After a storage of 1000 h, the PCE of PVK- and PEDOT:PSS- based devices dropped to 82.5% and 56% respectively, and demonstrated that the PVK-based perovskite devices is more stable compared with the PEDOT:PSS-based devices.
研究领域:
材料科学
·
太阳能
·
有机电子
✓ 已验证
DOI
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Shijia Lu,Chenxiao Zhang,Huiru Wu,Junjie Wang,Jinglong Wang,Limei Zhao,Tianyang Ren,Guofei Li
Liver cancer is one of the malignant tumors with high morbidity and mortality worldwide and lacks effective clinical treatments. CD8 + T cell-based immunotherapy is the most promising option for the treatment of liver cancer, but its application is severely limited by low infiltration and functional exhaustion of CD8 + T cells in liver cancer tissues. To overcome this problem, in this study, pH/matrix metalloproteinase-9 (MMP-9) dual-responsive liposomes were constructed and co-loaded with a galectin-3 (Gal-3) inhibitor, GB1107, a programmed death receptor 1 (PD-1) inhibitor, BMS1166, and an androgen receptor (AR) antagonist, enzalutamide, for immunotherapy studies in liver cancer. Specifically, the co-loaded liposomes (GBE@LP) were produced with a one-two punch targeting antitumor immunity. First, GBE@LP exhibited prolonged circulation in the blood and accumulated in liver cancer tissues through the enhanced permeability and retention effect due to the presence of surface polyethylene glycol. After entering liver cancer tissues, GBE@LP responded to the low pH and overexpression of MMP-9 in the microenvironment to promote the release of GB1107, BMS1166, and enzalutamide, thus, realizing the intelligent release of drugs targeting liver cancer. Upon reaching liver tumor tissues, GB1107 can inhibit overexpressed Gal-3 to transform liver cancer from a “cold” to a “hot” tumor and enable deep penetration by CD8 + T cells. In addition, BMS1166 and enzalutamide inhibit PD-1 and AR in CD8 + T cells, reversing CD8 + T cell exhaustion and enhancing their killing activity. In vivo and in vitro experiments confirmed the significant antitumor effects of GBE@LP in liver cancer. These results indicate that our strategy is expected to reverse the “cold” tumor and immune exhaustion state of liver cancer, which not only improves therapeutic efficacy, but also provides a new paradigm for the exploration of liver cancer immunotherapy.
研究领域:
肿瘤学
·
药理学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Yanjuan Peng,Ziteng Wang,Wenhao Jiao,Xinxin Cheng,Jingjing Yang,Yonghong Hu,Li Mi
This article deals with the saturated solubility and solvation behavior of 2-bromo-9-fluorenone in 10 industrial common solvents and three binary mixed solvents by means of experimental and mathematical correlations. Solubility experiments were investigated using the static equilibrium method combined with high-performance liquid chromatography (HPLC) from 278.15 to 323.15 K at atmospheric pressure. The results showed that the maximum and minimum solubility of 2-bromo-9-fluorenone was 0.0526 (mole fraction) in 1,4-dioxane at 323.15 K and 0.0002 (mole fraction) in methanol at 278.15 K, respectively, and the solubility increased with the increase in temperature in all solvents. Furthermore, the solubility data of 2-bromo-9-fluorenone was analyzed using the Apelblat model, the Buchowski–Ksiazaczak λh model in pure solvents, CNIBS/R-K model, and the Jouyban–Acree model in binary solvent mixtures. By error comparison, the Apelblat model and the CNIBS/R-K model can better predict the solubility of 2-bromo-9-fluorenone. In addition, the solvation behavior was illustrated by the KAT-LSER model in pure solvents, and the result shows that the cohesive energy density in the solution is the main factor affecting 2-bromo-9-fluorenone. Therefore, the solubility data is very important for optimizing the extraction process and recrystallizing 2-bromo-9-fluorenone to obtain a higher yield in industry production.
研究领域:
化学工程
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
CORROSION
·
2013
·Vol. 69
·期 7
·672-680
·
IF 1.772
Jiang Y.Y.,Chen Y.,Ye Z.Y.,Chen H.,Zhang Z.,Zhang J.Q.,Cao C.N.
N-[2-[(2-aminoethyl) amino] ethyl]-9-octadecenamide (AAOA) has been synthesized in the lab, then tested as an effective and economic acid inhibitor for A3 steel in an acid medium with electrochemical techniques (electrochemical impedance and polarization curve). The results show that AAOA acts as a good mixed-type inhibitor for A3 steel in 1 M hydrochloric acid (HCl) solution, and the inhibition efficiency reaches 94% at the concentration of 200 mg/L. The corrosion current density of A3 steel increases with the increasing temperature from 20°C to 60°C. Meanwhile, Langmuir isotherm is found to provide the description of the adsorption behavior of AAOA, and the value of adsorption free energy (ΔG 0 ) is as low as low as −37.3 kJ/mol. One can assume the adsorption of inhibitor molecules on the metal surface is by chemisorption, which has been understood taking into account some other adsorption parameters (ΔH 0 , ΔS 0 ) and the activation parameters (E a , ΔH * , ΔG * ).
研究领域:
材料科学
·
腐蚀与防护
·
电化学
✓ 已验证
DOI
高影响因子
Oncology Letters
·
2021
·Vol. 21
·期 3
·1-1
·
IF 2.967
Huizhe Zheng,Bin Yan,Qi Wu,Jingli Zhang
Chemotherapy drug 5‑fluorouracil (5‑FU) is the first‑line treatment for colorectal cancer (CRC); however, 5‑FU resistance decreases CRC therapeutic efficiency. A previous study revealed that microRNA (miR)‑9‑5p serves an antitumor effect in CRC. However, the effect of miR‑9‑5p in CRC chemoresistance remains unknown. In the present study, two CRC cell lines, including HT‑29 and HCT‑116 cells, were used to investigate the impact of miR‑9‑5p in overcoming 5‑FU resistance. The results revealed that treatment with 5‑FU decreased CRC cell viability and upregulated miR‑9‑5p expression in both CRC cells. Knockdown of miR‑9‑5p decreased HCT‑116 cell sensitivity to 5‑FU and inhibited apoptosis. By contrast, miR‑9‑5p overexpression enhanced the sensitivity of HT‑29 cells to 5‑FU and induced apoptosis. Additionally, it was confirmed that miR‑9‑5p directly targeted high mobility group A2 (HMGA2). HMGA2 overexpression reversed miR‑9‑5p‑induced HT‑29 apoptosis. The present study indicated that miR‑9‑5p enhanced the sensitivity of CRC cells to 5‑FU via downregulating HMGA2 expression.
研究领域:
肿瘤学
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2024
·
IF 3.9
Xian Xiao,Qiang Gao,Lan-Ying Wang,Yun-Fei Zhang,Yan-Ping Luo
An aromatic ring-containing compound with a wide range of biological activities, 9-methylacridine (AD-9-Me) is a precursor for the synthesis of various drugs. However, its photoactivation properties and mechanism of damage as a photo activator against Aedes aegypti are unknown. The toxic effects of AD-9-Me on Aedes aegypti mosquitoes were determined under light and non-light conditions. The results showed that the toxicity of AD-9-Me to mosquito larvae was significantly higher than that of the dark treatment after 24 h of light exposure; AD-9-Me was mainly distributed in the midgut of larvae, after 24 h of treatment, it can cause an increase in calcium ion concentration, reactive oxygen species (ROS) eruption and ROS accumulation by blocking the ROS elimination pathway in midgut cells. This in turn caused an increase in protein carbonyl and malondialdehyde (MDA) content, a decrease in mitochondrial membrane potential (MMP), a disruption of the barrier function of midgut tissues, a significant decrease in midgut weight and chitin content, which induced the up-regulation of AeDronc , AeCaspase8 and AeCaspase7 genes, leading to apoptotic cell death. In this study, we confirmed that AD-9-Me has photoactivation activity and mainly acts on the midgut of mosquito larvae, which can generate a large amount of ROS in the cells of the midgut and induce apoptosis to occur, resulting in the disruption of the function of the tissues of mosquito larvae, accelerating the death and delaying the development of the mosquito larvae.
研究领域:
昆虫学
·
毒理学
·
生物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yuhao Zhu,Chenming Dai,Danyu Lv,Yongguang Guan
Nonionic oil-in-water (O/W) nanoemulsion provides potential to stabilize hydrophobic bio-functional components in aqueous medium. Understanding safety of nanoemulsion droplets via investigating in vitro cell uptake and cellular substructural changes is important to achieve their practical applications. Herein, we developed a nonionic O/W nanoemulsion to stabilize representative bio-functional hydrophobic component of 9′- cis -bixin at pH 3–7 and ultraviolet (UV)-induced degradation at 365, 302, and 254 nm. In vitro cell uptake demonstrated that Caco-2 cells adequately enriched 9′- cis -bixin through fast uptake of nanoemulsion droplets within 15 min. However, excessive nanoemulsion droplets greatly decreased cell survival rate, which was due to the potential destruction of cellular substructures of mitochondria, nuclear membrane, and cell membrane. Lower nanoemulsion concentration provided no significant effects on Caco-2 cell survival. This work provided objective understanding on bio-functional component stability by nanoemulsion with in vitro safety evaluation.
研究领域:
食品科学
·
生物医学工程
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Siyu Wu,Hongyun Ma,Xiaoyan Chen,Wen Zhong,Yingying Gu,Yuqing Miao,Yarui An
Here, a label-free immunosensor was conducted using rGO/Collard-like Bi 2 S 3 @Au nanocomposite system to detect carbohydrate antigen 19-9 (CA19-9) as a diagnostic biomarker for Pancreatic cancer. The unique morphology of Collard-like Bi 2 S 3 increases its specific surface area, which allows it to immobilize more materials and biomolecules on its surface. To realize the covalent bonding of bismuth-based materials with biomolecules, bismuth-based materials were functionalized by Au NPs. Besides, the superior electrochemical activity and large specific surface area of Bi 2 S 3 @Au improved the stability of the sensor, which made it an ideal candidate for regulating the sensing interface in this study. Meanwhile, reduced graphene oxide (rGO) with remarkable electrical conductivity to improve the current response of the sensing interface, thus broadening the linear range of the immunosensor. Based on the above materials, a label free electrochemical immunosensor was constructed for detecting CA19-9. Under optimal conditions, it displays good linear relationship within the range of 0.01 U mL −1 to 100 U mL −1 , the detection limit being 0.0012 U mL −1 . Additionally, the immunosensor prepared in this work is characterized by superior interference immunity, reproducibility and storage stability, and has yielded satisfactory recovery rate in testing the serum samples of patients.
研究领域:
生物传感器
·
癌症诊断
·
纳米材料
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）