ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Yao Pengnan,Wang Zhengjun,Ma Kefeng,Kong Jinming,Zhang Xueji
Foodborne pathogens, including bacteria, viruses, fungi, and parasites, commonly cause disease outbreaks through contaminated food or water. Escherichia coli ( E. coli ) tends to induce severe illnesses like hemorrhagic colitis and hemolytic uremic syndrome. To address detection challenges, lectins, natural glycoproteins isolated from plants, animals, or microorganisms, demonstrate unique advantages through their specific binding to carbohydrate moieties on pathogen surfaces. This biomolecular recognition mechanism establishes lectins as ideal bioreceptors for developing biosensing platforms targeting foodborne pathogens. In this study, a fluorescent sensor that mediates atom transfer radical polymerization (ATRP) through Concanavalin A (ConA) was developed. This sensor utilizes ConA’s ability to capture pathogens and E. coli ’s ability to catalyze ATRP to identify E. coli . First, ConA is modified onto magnetic beads for initial capture, followed by modification of 2-bromoisobutyryl bromide (BIBB) onto the surface of captured E. coli for subsequent ATRP. If the pathogen captured by ConA is not E. coli , the subsequent ATRP cannot proceed normally, and the sensor cannot generate a fluorescent signal. Under optimal conditions, this sensor demonstrated good linearity from 1.00 × 10 2 to 1.00 × 10 7 CFU/mL. The limit of detection has gone down to 8 CFU/mL. This solution has the advantages of high sensitivity, low cost, and easy operation compared to traditional solutions for bacteria detection, which provides an efficient, stable, and sensitive solution for the detection of E. coli. Graphical In this study, a fluorescent sensor that mediates atom transfer radical polymerization (ATRP) through Concanavalin A (ConA) was developed. The sensor works by modifying ConA on magnetic beads, which allows the capture of pathogens through the recognition of E. coli surface polysaccharides by lectin. Next, 2-bromoisobutyryl bromide (BIBB) was applied to the E. coli surface in preparation for the subsequent ATRP. This solution has the advantages of high sensitivity, low cost, and easy operation compared to traditional solutions for bacteria detection, which provides an efficient, stable, and sensitive solution for the detection of E. coli.
研究领域:
生物传感器
·
微生物学
·
食品安全
·
高分子化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2012
·Vol. 19
·期 4
·1-7
·
IF 1.733
Shi Xu Hua,Xu Hai Tao,Yan Tao,Yu Ri Wen,Xu Yin Peng,Chen Han Jia
Cyclic polystyrene with narrow molecular weight distribution was synthesized by intramolecular coupling of α-carboxyl-ω-butylamino heterotelechelic polystyrene using 2-chloro-1-methylpyridinium and triethylamine as the catalyst in highly dilute solution. The linear precursor α-carboxyl-ω-butylamine polystyrene was first prepared by atom transfer radical polymerization using 4-(chloromethyl)benzoic acid as the initiator and followed by nucleophilic substituted with n -butylamine. The results of IR, 1 H NMR, and gel permeation chromatography revealed that highly pure cyclic polystyrene was synthesized via a combination of intramolecular coupling and atom transfer radical polymerization, and can be isolated easily by column chromatography on silica gel using ethyl acetate as the eluant.
研究领域:
高分子化学
·
材料科学
✓ 已验证
DOI
高影响因子
Canadian Journal of Chemistry
·
2017
·Vol. 95
·期 5
·
IF 1.08
Lei Wang,Shaoqing Wen,Zhanxiong Li
A series of novel amphiphilic ABA-type poly(tridecafluorooctylacrylate)-poly(ethylene glycol)-poly(tridecafluorooctylacrylate) (henceforth referred to as p-TDFA-PEG-p-TDFA) triblock oligomers were successfully synthesized via atom transfer radical polymerization (ATRP) using well-defined Br-PEG-Br as macroinitiator and copper as catalyst. The block oligomers were characterized by Fourier transform infrared (FTIR) spectroscopy and 1H and 19F nuclear magnetic resonances (NMR). Gel permeation chromatography (GPC) showed that the block oligomers have been obtained with narrow molecular weight distributions of 1.22–1.33. X-ray photoelectron spectroscopy (XPS) was carried out to confirm the attachment of p-TDFA-PEG-p-TDFA onto the silicon substrate, together with the chemical compositions of p-TDFA-PEG-p-TDFA. The wetabilities of the oligomer films were measured by water contact angles (CAs). Water CAs of p-TDFA-PEG-p-TDFA film were measured and their morphologies were tested by atomic force microscopy (AFM). The result showed that the CAs of the oligomer films, which possess fluoroalkyl groups assembled on the outer surface, increase after heating due to the migration of fluoroalkyl groups and the resulted microphase separation of the p-TDFA-PEG-p-TDFA.
研究领域:
材料科学
·
表面化学
✓ 已验证
DOI
高影响因子
Nanoscale
·
2014
·Vol. 6
·期 11
·6141-6150
·
IF 6.739
Xinchao Yang,Nana Zhao,Fu-Jian Xu
Graphene oxide (GO) has been proven to be promising in many biomedical fields due to its biocompatibility, unique conjugated structure, easily tunable surface functionalization and facile synthesis. In this work, a flexible two-step method was first developed to introduce the atom transfer radical polymerization (ATRP) initiation sites containing disulfide bonds onto GO surfaces. Surface-initiated ATRP of (2-dimethyl amino)ethyl methacrylate (DMAEMA) was then employed to tailor the GO surfaces in a well-controlled manner, producing a series of organic–inorganic hybrids (termed as SS–GPDs) for highly efficient gene delivery. Under reducible conditions, the PDMAEMA side chains can be readily cleavable from the GO backbones, benefiting the resultant gene delivery process. Moreover, due to the conjugated structure of the graphene basal plane, SS–GPD can attach and absorb aromatic, water insoluble drugs, such as 10-hydroxycamptothecin (CPT), producing SS–GPD–CPT. The MTT assay and the simultaneous double-staining procedure revealed that SS–GPD–CPT possessed a high potency of killing cancer cells in vitro. With a high aqueous solubility and coulombic interaction with cell membrane, SS–GPDs may have great potential in gene/drug delivery fields.
研究领域:
生物材料学
·
分子生物学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Chao Yan,Yiqun Fang,Mengfan Yan,Weihong Wang,Yongming Song,Qingwen Wang
Balsa wood, despite being a cost-effective insulation material, has limited applications due to its inherent hydrophilicity. This study introduces an innovative technique for the in-situ growth of low surface energy long alkyl chain segments on balsa wood using the atom transfer radical polymerization (ARGET-ATRP) method, which not only provides durable and stable hydrophobicity but also preserves its natural texture, mechanical strength, and thermal insulation. The resulting hydrophobic wood demonstrated exceptional hydrophobicity in diverse environments, with a water contact angle up to 141.3° and a contact angle decrease of no more than 10.47 % after 150 min, confirming its hydrophobic stability. Furthermore, axial thermal conductivity of the wood was as low as 0.1012 W/m·K, the axial compressive strength decreased by only 12.8 %, further evidencing the retention of its mechanical properties. This simple yet effective strategy of in-situ alkyl chain growth on balsa wood not only preserves its structure but also opens new avenues for the multifunctional development of biomaterials.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2012
·
IF 3.628
Xiaoyun Qiu,Xueqin Ren,Shuwen Hu
An independently temperature- and pH-responsive membrane was developed by simultaneously grafting poly( N -isopropylacryamide) (PNIPAAm) and poly[(2-(diethylamino)ethyl methacrylate] (PDEAEMA) from different sides of a crosslinked cellulose membrane. The synthesis was simplified by using surface-initiated activators regenerated by electron transfer for atom-transfer radical polymerization in a diffusion device. The grafted membrane was heterostructured. The grafted polymer layer thickness was linearly related to reaction time. The wettabilities of the double-membrane sides responded individually and reversibly to temperature and pH. The surface grafted with PNIPAAm shifted from hydrophilicity to hydrophobicity above the lower critical solution temperature. The PDEAEMA side was hydrophilic in acidic aqueous solution and hydrophobic under basic conditions. This dual-response cellulose membrane has potential applications in water treatment, separations, and other membrane applications.
研究领域:
材料科学
·
化学工程
·
膜技术
✓ 已验证
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Ming Li,Shiyu Fu
Wood is actually a natural polymers composite that is easy to process, mainly constituted of cellulose, hemicellulose and lignin. However, most wood-based functional materials are prepared by physical methods, which undoubtedly limit the application of wood-based materials. Herein, the wood aerogel (WA) was obtained from wood by delignification and freezing dried. The WA then was fabricated into an unexpected photochromic wood aerogel (PWA) via surface-initiated atom transfer radical polymerization (SI-ATRP) covalently grafting poly{6-[4-(4-methoxyphenyl-azo)phenoxy]hexyl methacrylate} (PMAZOM). Both WA and PWA showed good compression resilience. The hydrophobicity of PWA was obviously enhanced because of the surface modification. The color of PWA can change from yellow to orange-red after being irradiated by UV light for 30 s. The color of sample can be reversibly changed to yellow under sunlight for 200 s. The lightweight and photosensitive wood aerogel is potential to apply for optical information storage materials and photosensitive devices.
研究领域:
材料科学
·
高分子化学
·
纳米材料
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智能材料
·
可持续材料
✓ 已验证
DOI
高影响因子
MATERIALS SCIENCE FORUM
·
2010
·
IF 0
Xin Wang,Yuan Yuan Dou,Mei Shan Pei,Xin De Tang
Biocompatible and biodegradable synthetic materials have attracted considerable attention during the past two decades. In this work, a series of amphiphilic triblock copolymers containing D-galactopyranose were synthesized by atom transfer radical polymerization (ATRP). The macroinitiator was prepared by the esterification between poly(ethylene glycol) monomethyl ether with a number-average molecular weight of 1200 g/mol (MeOPEO-1200) and 2-bromoisobutyryl bromide. The sugar-bearing monomer, 6-O-methacryloyl-1,2;3,4-di-O-isopropylidene-D-galactopyranose (MAIPG) was polymerized using PMDETA/CuBr as catalytic system and anisol as solvent. The chemical structure and composition of the resultant polymer MeOPEO-b-PMAIPG were verified by 1H NMR. The molecular weights and their polydispersities were characterized by gel permeation chromatography (GPC). The results indicate that the polymerization follows the mechanism of ATRP. The amphiphilic copolymers can self-assembly to from micelles with PMAIPG as the core and PEO as the corona, which have potential applications as biomaterials or controlled release drug delivery systems.
研究领域:
高分子化学
·
生物医学工程
·
药物递送
·
材料科学
·
大分子化学
✓ 已验证
DOI
Polymer Chemistry
·
2015
·Vol. 6
·期 18
·3480-3488
·
IF 5.52
Leli Wang,Jianan Shen,Yongjun Men,Ying Wu,Qiaohong Peng,Xiaolin Wang,Rui Yang,Khalid Mahmood,Zhengping Liu
Ionic liquid was employed as a reaction medium to prepare starch-based macro-initiator for atom transfer radical polymerization (ATRP), due to its high dissolubility for starch and chemical inertness. Starch macro-initiators with varying degrees of substitution (DS) were successfully synthesized in ionic liquid 1-allyl-3-methylimidazolium chloride ([AMIM]Cl) by homogeneous esterification with 2-bromoisobutyryl bromide at room temperature without the use of any additional catalysts. Starch-based copolymers (Starch-g-PS and Starch-g-PMMA) were prepared at the molecular level via homogeneous ATRP using CuBr/PMDETA and CuBr/BPY as catalysts. Compared with heterogeneous surface-initiated polymerization, the graft density and graft ratio were significantly improved. The structure and thermo behaviour of the graft copolymers were characterized by 1H NMR, FTIR and TGA. The molecular weights of the grafted polymer chains were analyzed by gel permeation chromatography (GPC) after hydrolyzing of the starch backbone from the copolymer. The effects of molar ratio of monomer to initiator, solvent, ligand and temperature on the graft polymerization were investigated as well.
研究领域:
高分子化学
·
材料科学
·
绿色化学
·
生物基材料
·
聚合反应工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Tingting Ding,Ruixia Liu,Xiaofan Yan,Zuoyu Zhang,Fuquan Xiong,Xingong Li,Zhiping Wu
It is a promising idea to graft zwitterionic polymers onto lignin and prepare lignin-grafted-poly [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide (Lignin-g-PDMAPS) thermosensitive polymer with the upper critical solution temperature (UCST). In this paper, an electrochemically mediated atom transfer radical polymerization (eATRP) method was used to prepare Lignin-g-PDMAPS. The structure and property of the Lignin-g-PDMAPS polymer were characterized by the fourier transform infrared spectrum (FT-IR), nuclear magnetic resonance (NMR), X-ray electron spectroscopy (XPS), dynamic light scattering (DLS), differential scanning calorimeter (DSC). Furthermore, the effect of catalyst structure, applied potential, amount of Lignin-Br, Lignin-g-PDMAPS concentration, NaCl concentration on UCST of Lignin-g-PDMAPS were investigated. It was worth noting that polymerization was well controlled when the ligand was tris (2-aminoethyl) amine (Me 6 TREN), applied potential was −0.38 V and the amount of Lignin-Br was 100 mg. And the UCST of the Lignin-g-PDMAPS aqueous solution (1 mg/ml) was 51.47 °C, the molecular weight was 8987 g/mol, and the particle size was 318 nm. It was also found that the UCST increased and the particle size decreased with the Lignin-g-PDMAPS polymer concentration increased, and the UCST decreased and the particle size increased with the NaCl concentration increases. This work investigated UCST-thermoresponsive polymer which possessed lignin main chain combining the zwitterionic side chain, and provided a new way for development of lignin based UCST-thermoresponsive materials and medical carrier materials, in addition to expand the scope of eATRP.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Tongjun Yang,Rongli Li,Mingtao Ding,Hong Yu,Lihua Zhang,Haibo Xie
Chitin nanocrystals (ChNCs), prepared from a down-sizing process from chitin, have recently captured great attention to access sustainable nanomaterials. The surface modification of ChNCs is crucial to regulate the surface physicochemical properties and introduce specific functions, thus satisfying their diverse applications. In this study, aldehyde-functionalized ChNCs (ChNCs-PVMA) with enhanced hydrophobicity were developed via surface-initiated activators regenerated by electron transfer for atom transfer radical polymerization (SI-ARGET ATRP) of a lignin-derived polymerizable aldehyde monomer, vanillin methacrylate (VMA). The monomer conversion was determined by 1 H NMR spectroscopy of the reaction mixture based on the change of the relative ratio of VMA and solvent signals. The prepared ChNCs-PVMA were systematically characterized by FTIR, CP/MAS 13 C NMR, XPS, XRD, DSC, TGA, and TEM. The dispersibility of ChNCs and ChNCs-PVMA in water and DMF was evaluated by dynamic light scattering and visual observation, indicating good dispersion of ChNCs-PVMA in organic solvents. Furthermore, based on the available aldehyde groups, the ChNCs-PVMA was reacted with amino acids via Schiff base reaction, demonstrating a rich follow-up chemistry towards diverse functions by the reactive aldehyde groups.
研究领域:
材料科学
·
化学工程
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Xinyue Liang,Jingwei Zhang,Xiaoxiao Wu,Xiaotong Fang,Pengyu Qu,Guowei Wang
Polyolefins (POs) are key materials in industrial applications, and the development of high-performance and high-value-added POs is always a significant and challenging research focus. The major challenge lies in addressing compatibility between non-polar POs and polar functional components. We herein reported a facile strategy to prepare PO-based nano-objects using an activator regenerated by electron transfer atom transfer radical polymerization mediated polymerization-induced self-assembly (ARGET ATRP PISA) process based on hydrogenated polyisoprene- b -poly (glycidyl methacrylate) (HPI- b -PGMA)/poly (glycidyl methacrylate) (PGMA) blends. The dynamic light scattering (DLS) and transmission electron microscopy (TEM) revealed effective control over a wide range of hydrodynamic diameters of stabilized spherical nano-objects. The thermogravimetric analysis (TGA) and microscale combustion calorimetry (MCC) demonstrated the efficient and controllable introduction of phosphoric acid (H 3 PO 4 ) into nano-objects, with POs serving as the shell and flame retardants forming the core. This work was hoped to provide a versatile route to PO-based nano-objects and open a novel avenue for designing PO-based additives.
研究领域:
高分子化学
·
材料科学
·
纳米技术
·
化学工程
·
大分子化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）