BIOELECTROCHEMISTRY
·
2019
·
IF 4.474
Shuan Zhang,Chunyan Zhang,Yilei Jia,Xiaobo Zhang,Yunhui Dong,Xinjin Li,Qing Liu,Yueyun Li,Zengdian Zhao
A sandwich-type electrochemical immunosensor was fabricated for the quantitation of alpha fetoprotein (AFP). To this end, the Au@Pt dendritic nanorods loaded with amino functionalized molybdenum selenide nanosheets (Au@Pt 41 DNRs/NH2-MoSe2 NSs) with enhanced peroxidase-like properties were selected as the secondary antibody label (Ab2) to achieve signal amplification. The as-obtained Au@Pt DNRs/NH2-MoSe2 NSs exhibited better catalytic activity toward hydrogen peroxide reduction and offered rich anchors for bioconjugation . Meanwhile, gold nanoparticles anchored on an amino functionalized graphene (Au NPs/NH2-GS) composite with admirable conductivity and biocompatibility was used as the matrix material to improve sensitivity. Under optimal conditions, amperometric current responses had a good linear relationship with the logarithm values of AFP concentration in the range 10 fg mL –1 to 200 ng mL –1 with a detection limit of 3.3 fg mL –1 (S/N = 3). Additionally, the immunosensor had excellent reproducibility, selectivity, and stability, which indicated superior performance for AFP detection compared with previous reports. The satisfactory results of human serum samples analysis showed that the designed immunosensor has potential applicability for the sensitive detection of other tumor markers.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Ziyin Yang,Xiaohui Zheng,Jianbin Zheng
A novel strategy is developed to synthesize three-dimensional porous Au@Pt core-shell nanoflowers supported on GO (Au@Pt-nFs/GO) for enhanced electrochemical sensing of hydrazine (N 2 H 4 ). Au@Pt-nFs/GO nanocomposite was synthesized through a facile and green method, where ascorbic acid and chitosan were employed as the reductant and linking agent, respectively. The experiment results revealed that a large numbers of Au@Pt-nFs with Au cores and Pt dendritic shells were distributed on the surface of GO sheets. Meanwhile, Au@Pt-nFs/GO with large surface area, abundant active sites and open structure reduced the overpotential and accelerated kinetics of N 2 H 4 oxidation, therefore making the sensor based on Au@Pt-nFs/GO exhibit an excellent performance for N 2 H 4 analysis with a wide linear range of 0.8 μM–0.429 mM, a high sensitivity of 1695.3 μA mM −1 cm −2 and a low detection limit of 0.43 μM (S/N = 3). Moreover, the sensor also exhibited good selectivity, repeatability and stability. Application of the sensor for the detection of N 2 H 4 in tap water samples was demonstrated.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Guoni Huang,Jiezhen Pan,Junfang Zhu,Ye Zhang,Xiaohe Zhang,Shihua Luo
In this study, we introduce an ultrasensitive electrochemical RNA biosensor based on hyperbranched primer exchange reaction (HPER) cascade amplification and Au@Pt@UiO-66-NH 2 nanozymes. This strategy employs catalytic hairpin assembly (CHA) for precise target RNA recognition, which trigger the immobilization of HPER products onto the electrode surface, thereby providing binding sites for nanozymes. Then, the sDNA@Au@Pt@UiO-66-NH 2 signal probes (sDAPU probes) attach to the HPER scaffolds through base-pairing interactions, facilitating signal amplification. The nanozymes catalyze the decomposition of hydrogen peroxide into H 2 O and O 2 , producing a significantly amplified electrochemical signal. By combining CHA-HPER cascade amplification with the catalytic activity of sDAPU probes, the established biosensor achieves remarkable sensitivity, with a detection limit as low as 14.68 aM, and high specificity that enables the differentiation of non-target pathogen RNA. Furthermore, the biosensor also demonstrates excellent recovery rates in serum samples. Therefore, this electrochemical strategy shows great potential as a diagnostic tool for ultrasensitive Mycobacterium tuberculosis detection.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Limin Yang,Heng Guo,Ting Hou,Jingang Zhang,Feng Li
Breast cancer (BC) is the most common cancer in the world and circulating tumor cells (CTCs) are reliable biomarkers for early breast cancer diagnosis in a non-invasive manner. However, effective isolation and sensitive detection of BC-CTCs by portable devices in human blood samples are extremely challenging. Herein, we proposed a highly sensitive and portable photothermal cytosensor for direct capture and quantification of BC-CTCs. To achieve efficient isolation of BC-CTCs, aptamer functionalized Fe 3 O 4 @PDA nanoprobe was facilely prepared through Ca 2+ -mediated DNA adsorption. To further detect the captured BC-CTCs with high sensitivity, multifunctional two-dimensional Ti 3 C 2 @ [email protected] nanozyme was synthesized, which not only possessed superior photothermal effect but also exhibited high peroxidase-like activity for catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) to produce TMB oxide (oxTMB) with a strong photothermal characteristic, combining with Ti 3 C 2 @ [email protected] to synergistically amplify the temperature signal. Moreover, numerous Ti 3 C 2 @ [email protected] nanocomposites would be selectively attained on the BC-CTCs surface through multi-aptamer recognition and binding strategy, which further enhanced the specificity and facilitated signal amplification . Therefore, direct separation and highly sensitive detection of BC-CTCs was successfully achieved in human blood samples. More significantly, the controlled release of the captured BC-CTCs without affecting cell viability could be straightforwardly realized by a simple strand displacement reaction. Thus, with the distinct features of portability, high sensitivity, and easy operation, the current method holds great promise for early diagnosis of breast cancer.
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PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Xiao Liang,Cheng-Guo Zhan,Juan Yang,Ji-Tao Li,Wen-Bo Zhao,De-Zhi Yang,Ya-Ling Yang,Zi-Tao Zhong
Transferrin (TRF) is the main iron-containing protein in plasma, responsible for carrying iron from digestive absorption and released from the erythrocyte degradation. The plasma TRF levels can be used to diagnose anemia and monitor treatment. Herein, a sandwich type biosensor was developed for the sensitive and efficient TRF detection utilizing the affinity of boronic acid and specific recognition of antibody. TRF can be captured by the facilely synthesized phenylboronic acid decorated SiO 2 @Fe 3 O 4 magnetic nanoparticle (PBA-SiO 2 @Fe 3 O 4 ), and then the antibody functionalized Au,Pt,Cu@polydopamine (Ab-Au,Pt,Cu@PDA) with peroxidase-like activity was bond to TRF to form the sandwich conjugate. After the prompt magnetic separation and catalysis with the chromogenic substrates (TMB and H 2 O 2 ), the distinct color development can be observed and measured for the TRF quantification. The proposed method shows satisfied performance with a detection range of 0.128–2000 ng/mL and LOD of 0.051 ng/mL. Moreover, this approach possesses excellent stability and simple operation, without complicated sample pretreatment. Consequently, this biosensor poses a significant potential for the diagnosis and monitoring of abnormal TRF levels-related diseases.
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PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Lei Zhang,Huiqiang Huang,Mengyao Liao,Chenghua Luo,Qiuyue Wu,Rentang Huang,Hongmei Liu,Xiangsheng Zhao,Shumei Wang
The enzyme-linked immunosorbent assay (ELISA) is a powerful and high-throughput method for detecting aflatoxin B 1 (AFB 1 ) in food. However, the susceptibility of native enzymes to environmental factors limits their potential applications. This study introduces the combined use of precious metal nanozymes as alternative catalysts to improve the performance of traditional ELISA. Au@Pt can be linked to antibodies to construct recognition probes, onto which platinum nanoparticles (PtNPs) are ingeniously deposited in situ to amplify the detection signal, thereby increasing the sensitivity. The proposed PtNP-enhanced Au@Pt-ELISA exhibited excellent resistance against matrix interference for AFB 1 detection in lotus seed powder and achieved a detection limit of 1.6 pg/mL (0.32 μg/kg), which was approximately six times lower than that of traditional ELISA. Furthermore, the results of the proposed approach were strongly correlated with LC–MS/MS analysis results for real samples ( r = 0.9888, n = 13). This study offers a new strategy for AFB 1 monitoring in a complex matrix.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jiawen Li,Huali Jin,Min Wei,Wenjie Ren,Jinshui Wang,Yurong Zhang,Ligen Wu,Baoshan He
In this work, a sensitive dual mode competitive electrochemical immunosensor based on differential pulse voltammetry (DPV) and square wave voltammetry (SWV) response modes for quantitative determination of dibutyl phthalate (DBP) was successfully established. According to diethylenetriamine (DETA) assisted hydrothermal strategy, a novel N-doped graphene/CoSe 2 nanobelt composite was prepared. Afterwards, PEI functionalized NG-CoSe 2 and gold nanowires (AuNWs) were further compounded as a preliminary signal amplification platform for immunosensor. Thionine (Thi)-loaded [email protected] and secondary antibody (Ab 2 ) connected as a signal label. The immobilized coating antigen DBP-BSA and the DBP compete for limited antibody binding sites. The concentration of DBP can be determined by DPV detect the Thi signal loaded on the [email protected] 2 label. It can also be determined by SWV mode to detect the current generated by the reaction system of H 2 O 2 and o-phenylenediamine (o-PD) catalyzed by [email protected] with peroxidase like properties. Under optimum conditions, the linear ranges of the DPV and SWV modes were 1 pg mL −1 ∼1 μg mL −1 . And LOD were 0.486 pg mL -1 (DPV) and 0.711 pg mL -1 (SWV), respectively. Besides, it exhibited outstanding selectivity, reproducibility, stability and can be used for sensitive detection of DBP in real samples.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Pengfei Pang,Xia Teng,Meng Chen,Yanli Zhang,Hongbin Wang,Chun Yang,Wenrong Yang,Colin J. Barrow
An ultrasensitive enzyme-free electrochemical immunosensor was developed for microcystin- LR (MC-LR) detection based on a unique competitive detection scheme using molybdenum disulfide (MoS 2 ) nanosheets/BSA-stabilized gold nanoclusters (AuNCs) composite and Au core/Pt shell nanoparticles (Au@PtNPs). MoS 2 /AuNCs nanocomposite was used for a platform for immobilizing more antibody due to its large surface area and excellent biocompatibility. This enzyme-free immunosensor is coupled with a robust immunosandwich format employing Au@PtNPs-labeled antibody as a non-enzymatic reporter. Due to high catalytic efficiency and good stability of Au@PtNPs, Au@PtNPs-immobilized antibody competed for target antigen to form an antibody-antigen immunocomplex and further amplified the electrochemical signal. Thus, the concentration of the MC-LR is directly proportional to the electrochemical signal of immunosensor. Under optimized conditions, the proposed enzyme-free immunosensor was employed to detect MC-LR and exhibited a wide linear range of 1.0 ng L −1 –1.0 mg L −1 with low detection limit of 0.3 ng L −1 ( S/N = 3). This ultrasensitive and high specific nonenzymatic electrochemical immunoassay shows promising application in environmental and food monitoring.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hongbo Li,Hao Wang,Meiyi Cao,Xing Tong,Shuo Wang,Mingfei Pan
The fluoroquinolone-enrofloxacin (ENR) residue in animal-derived foods poses a significant threat to human health, thereby necessitating developing precise, efficient, and user-friendly detection methods. This study developed a dual-mode fluorescence-colorimetric immunosensing platform using the composite of bimetallic nanoparticles integrated with metal-organic frameworks (Au/Pt NPs@NH 2 -MIL-53(Al)). The Au/Pt NPs with biocompatibility and peroxidase-like activity were modified onto fluorescent NH 2 -MIL-53(Al) and conjugated with Ab 2 to construct signal probes. The platform enables dual-mode quantitative analysis through the competitive binding of ENR and ENR-BSA conjugate to signal probes. It demonstrated a wider linear range (0.08–180 ng/mL) and high sensitivity [LOD: 0.068 ng/mL (fluorescence), 0.066 ng/mL (colorimetric), and 0.074 ng/mL (smartphone-assisted color recognition)]. In real sample analyses, the recoveries ranged from 86.7 % to 106.7 %, with satisfactory stability (RSD ≤ 4.4 %, n = 3). The dual-signal mode facilitates results self-calibration, enhancing reliability, while smartphone-assisted color recognition enables on-site, convenient screening, offering great potential for ENR detection in complex food matrices.
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PubMed
高影响因子
Biomedical Optics Express
·
2025
·Vol. 16
·期 5
·2033-2046
·
IF 3.2
Binbin Zeng,Huaiwu Fu,Cong Wu,Xudong Zhang,Tianran Li,Qiong Xu,Yayun Qian,Yayun Qian,Ruirui Yang,Ruirui Yang
In this study, we constructed a SERS assay platform that can be used for the ultrasensitive detection of cerebral infarction (CI)-associated miR-106a-5p based on the recognition competition strategy and the highly efficient catalytic activity of Au@Pt NRs nanoenzymes. When the target miR-106a-5p is present in the detection solution, it binds to the nucleic acid aptamer (cDNA), leading to the detachment of its complementary strand (H1) carrying Au@Pt NRs from the surface of AuNHs, and the oxidation of TMB is reduced, resulting in the decrease of SERS signal. In addition, this method enables single-step, ultrasensitive detection of target miRNAs in patient serum with a limit of detection (LOD) of 0.96 fM, which is consistent with the results of real-time quantitative polymerase chain reaction (qRT-PCR) and fluorescence. This method provides a novel approach for the effective detection of miRNAs, which is of great clinical value for the early detection of CI.
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高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 11
·1475-1482
·
IF 2.378
Xiaoyan Mo,Zunyi Wu,Jianfeng Huang,Guangying Zhao,Wenchao Dou
A sensitive and quantitative sandwich electrochemical immunosensor was constructed for the detection of Escherichia coli O157:H7 (E. coli O157:H7) using immune gold@platinum nanoparticles (Au@Pt), neutral red (NR), reduced graphene oxide nanocomposite (rGO) and regenerative leucoemeraldine base polyaniline (PANI)/gold nanoparticles (AuNPs) modified screen-printed carbon electrode (SPCE). The batch production advantages of the SPCE were retained, its disposable characteristic was replaced by the possibility for reuse. The anti-E. coli O157:H7 monoclonal antibody (Ab1) was automatically adsorbed on the SPCE-PANI-AuNPs platform through the amino and AuNPs interaction. rGO-NR-Au@Pt as the non-enzyme signal label can enhance the performance of the immunoassay for the catalytic reduction of H2O2 and amplify the current signal. All characterization steps were monitored by cyclic voltammetry (CV). Based on electrochemical detection of E. coli O157:H7, the linear range was from 8.9 × 103 to 8.9 × 109 CFU mL−1, and limit of detection (LOD) was 2.84 × 103 CFU mL−1. To ensure that the immunosensor can be used repeatedly, ultrasound sonication was used to remove as many surface-bound antibodies as possible. This allows Ab1 to bind to the SPCE-PANI-AuNPs again after the electrode was re-incubated with the antibody solution, and hence, the displacement assay can be repeated and the sensor reused. Furthermore, it was demonstrated that the microchips can be regenerated and reused at least 5 times.
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高影响因子
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