SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaojie Shi,Huimin Liu,Mei Zhang,Fengzhen Yang,Jiansen Li,Yemin Guo,Xia Sun
An ultrasensitive aptasensor based on luminol-hydrogen peroxide (H 2 O 2 ) electrochemiluminescence (ECL) system was proposed for determination of profenofos. AuNPs decorated with MWCNTs ( [email protected] ) were used to provide a favorable microenvironment for luminol loading and improve the ECL signal due to their good biological activity, excellent electrical conductivity and high specific surface area. Gold and silver core-shell nanoparticles ( [email protected] ) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL signal again. Under optimal conditions, the proposed ECL aptasensor obtained an extremely low detection limit of 5.32 fg/mL (S/N = 3) with a good detection range from 100 fg/mL to 1 μg/mL. Moreover, the proposed aptasensor had excellent specificity, outstanding repeatability and favorable stability. Finally, the proposed ECL aptasensor was used for the detection of profenofos in real samples and the recoveries ranged from 94% to 102%. This strategy could provide a good reference for developing effective aptasensors for detection of other small molecules and toxins.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-9
·
IF 5.833
Wang Lina,Zhang Li,Yu Ying,Lin Bixia,Wang Yumin,Guo Manli,Cao Yujuan
A sensitive electrochemical strategy was established for kanamycin determination. A specific aptamer was modified on the electrode as the probe, followed by a cyclic hybridization chain reaction (HCR) with methylene blue, causing an increasing signal response. In the presence of kanamycin, it can initiatively convolve the aptamer and prevent further DNA assembling, resulting in a signal distinction sensitive to the target amount. However, the signal reproducibility is low. To improve the precision, the HCR procedure was investigated. The results demonstrated that the optimal amount of assembled DNA is 12-fold to that of aptamer. This amount was then controlled in further assays. Admittedly, controlled DNA assembling commonly indicates a limited signal amplification. To further enhance the sensitivity, a nanocomposite based on MoS 2 and AuNPs was modified on the electrode. The results of the assay proved that the signal distinction sensitive to target amount increased by 50%. A linearity range is obtained from 0.01 nM to 1.0 μM of kanamycin, and the LOD is 8.4 pM. Subsequently, this strategy was employed to detect kanamycin in chicken liver and milk sample; the recovery results suggest that it possess a satisfactory application prospect in analysis of agricultural products. Graphical abstract
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高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Xiangyu Ma,Kun Qian,Onome Ejeromedoghene,Martha Kandawa-Schulz,Wei Song,Yihong Wang
In this work, we report AuNPs-decorated pyrolyzed Co-BDC nanosheets (p-Co-BDC/AuNPs) as high-performance electrocatalyst for developing an electrochemical platform. p-Co-BDC/AuNPs as a new electrocatalyst showed superior electrocatalytic activity towards the electrochemical oxidation of methylene blue (MB). Besides, magnetic p-Co-BDC/AuNPs can be well immobilized on the magnetic glassy carbon electrode without further assistance. The oxidation of MB can be reduced by ascorbic acid . Inspired by this phenomenon, an electrochemical biosensor was constructed based on multiple signal amplification for the diagnosis of miRNAs . Firstly, p-Co-BDC/AuNPs enhanced the electrochemical oxidation of MB. Then, strand displacement amplification reaction can form lots of double helix structure DNA to embed more MB molecules. Finally, ascorbic acid in the electrolyte was utilized to reduce the oxidation of MB and improve the electrochemical signal of MB electro-oxidation. The linear detection range for the detection of miRNAs is 100 aM to 10 nM, and the limit of detection is 86 aM. Furthermore, the constructed biosensor also displayed satisfactory selectivity , good reproducibility, and excellent recovery in the detection of real samples. We are convinced that our proposed multiple signal amplification strategy will provide more promising methods for the diagnosis of cancer.
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高影响因子
CELLULOSE
·
2019
·Vol. 26
·期 6
·4191-4204
·
IF 3.917
Cheng Deshan,Bai Xue,He Mantang,Wu Jihong,Yang Hongjun,Ran Jianhua,Cai Guangming,Wang Xin
Depositing anisotropic noble metal nanoparticles with high uniformity and yield on flexible substrates is the determining factor for surface enhanced Raman spectroscopy (SERS) detection. In this work, flexible, durable and sensitive SERS substrates were fabricated by in situ reduction of Ag nanoparticles on polydopamine templated cotton fabrics (CF) as catalytic hotspots to enhance the following deposition of Au nanoparticles. The coated CF were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy and X-ray diffraction to understand the surface morphology, chemical composition and crystalline structure, respectively. The SEM images indicate that the nanoparticles are dispersed evenly on the CF. 4-Mercaptobenzoic acid (4-MBA) has been used as the probe molecule to evaluate the sensitive and reproducible SERS properties of the as-fabricated SERS substrate. The as-prepared SERS substrates were demonstrated to detect carbaryl pesticides on a cucumber, and carbaryl with the concentration of as low as 10 −6 M (0.20 ppm) could be detected to ensure food safety.
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ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Wenjie Liu,Qiaofang Shi,Gang Zheng,Jun Zhou,Ming Chen
In this paper, gold nanoparticles (AuNPs) modified by thiolated calix[n]arene (TCnA, n = 4, 6, 8) were anchored on graphene nanosheets (GN) by π-π stacking interaction between TCnA molecules and GN. Testing of various electrochemical techniques display that the AuNPs@TCnA/GN modified glass carbon electrodes (GCE) show good electrocatalytic property , molecular recognition and enrichment performance toward dopamine (DA) and acetaminophen (AC), and exhibit high current response. In all of modified electrodes , AuNPs@TC8A/GN/GCE shows an outstanding electrochemical response for DA and AC with high sensitivity of 5.08 and 9.76 mA mmol −1 L cm −2 , respectively. The analytical curves for DA obtained by differential pulse voltammetry (DPV) are linear in the range from 0.5 to 150 μM and from 0.3 to 1.0 mM. The linear range for AC obtained by DPV is from 0.5 to 120 μM. Besides, AuNPs@TC8A/GN/GCE can be applied to determinate of DA and AC simultaneously, implying the prospect to develop a dual sensor under the same construction platform.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Pan Wang,Mingliang Du,Ming Zhang,Han Zhu,Shiyong Bao,Meiling Zou,Tingting Yang
Here we proposed a facile strategy to synthesize multi-layered gold nanoparticles/polyaniline/halloysite nanotubes (AuNPs/PANI/HNTs) nanostructures used for electrochemical sensors. The PANI/HNTs were firstly obtained by making use of the in situ polymerization as well as employing the thioglycollic acid (TA) as the dopant, thereafter, Au ions were anchored to TA and then reduced by PANI. Field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) observations implied that a large amount of uniform AuNPs were immobilized on the PANI/HNTs. The as-prepared AuNPs/PANI/HNTs nanostructures were also characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), and the results confirmed the successful synthesis of AuNPs/PANI/HNTs nanostructures and explained the reactions in depth as well. Further investigations suggested that the AuNPs/PANI/HNTs nanostructures with well-separated AuNPs exhibited high electrochemical performance as sensors to detect hydrogen peroxide (H 2 O 2 ).
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NANO
·
2015
·
IF 1.09
YALI MAO,HUANBAO FA,YANMEI CHENG,YANQIU DU,WEI YIN,CHANGJUN HOU,DANQUN HUO,DONG ZHANG
A novel sensitive and elective electrochemical sensor was developed to detect methyl parathion (MP) based on a glassy carbon electrode (GCE) modified with gold nanoparticles ( AuNPs )/graphene nanocomposites film. The AuNPs were modified onto graphene sheets using NaBH 4 as a reductant. The obtained AuNPs /graphene nanocomposites were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The electrochemical behavior of MP and interference studies were then investigated. Compared with metal ions and nitroaromatic compounds, which exist in environmental samples, the AuNPs /graphene/GCE exhibited high adsorption and strong affinity toward MP. Under optimized conditions, the oxidation peak current of MP was linear to its concentration within the range of 4.0 × 10 -7 –8.0 × 10 -5 M, with a detection limit ( S/N = 3) of 8.5 × 10 -8 M. These results indicated that the AuNPs /graphene nanocomposites displayed a synergic effect involving the catalytic characteristics of graphene and AuNPs nanocomposites, which can effectively improve the electrochemical properties of MP. Furthermore, the AuNPs also enhanced sensor sensitivity to MP. Therefore, the AuNPs /graphene/GCE could be a promising sensor for the fast, sensitive and selective detection of MP in real samples.
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高影响因子
LUMINESCENCE
·
2017
·Vol. 33
·期 1
·119-124
·
IF 1.509
Nan Liu,Juan Hao,Keying Cai,Mulan Zeng,Zhenzhong Huang,Lili Chen,Bingxian Peng,Ping Li,Li Wang,Yonghai Song
A novel ratiometric fluorescence nanosensor for superoxide anion (O 2 •− ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb 3+ and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O 2 •− . The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O 2 •− demonstrated high sensitivity and selectivity with a wide linear response range (14 nM–10 μM) and a low detection limit (4.7 nM).
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yu Li,Ying Chen,Dongmei Deng,Liqiang Luo,Haibo He,Zhenxin Wang
In this work, a water-dispersible graphene/amphiphilic pyrene derivative nanocomposite has been synthesized for electrochemical immunosensing carcinoembryonic antigen (CEA). Water-soluble 4-armed poly(ethylene glycol)-NH 2 is immobilized on graphene through noncovalent functionalization with the assistance of pyrene as an anchor group. The noncovalent functionalization strategy can significantly improve the hydrophilicity of graphene and loading capacity of gold nanoparticles (AuNPs) on graphene, resulting in efficient immobilization of antibodies on the nanocomposite. The morphology and conductivity of the as-prepared nanocomposites were characterized by scanning electron microscopy, Fourier transform infrared and UV–vis spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). CEA monoclonal antibody (anti-CEA) is immobilized on AuNPs via gold-thiol chemistry to construct the electrochemical immunosensing platform, and the surface binding events between anti-CEA and CEA is achieved by monitoring the change of the electrochemical impedance response. Under optimum conditions, the modified electrode exhibits a linear impedance response to CEA in a wide range of 0.1–1000 ng mL −1 ( R 2 = 0.9959) with low detection limit of 0.06 ng mL −1 ( S/N = 3).
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LUMINESCENCE
·
2024
·Vol. 39
·期 7
·e4829
·
IF 3.2
Juanjuan Cheng,Xinru Wei,Lun Wang,Hongqi Chen
A ratio luminescence probe was developed for detecting Staphylococcus aureus ( S. aureus ) based on luminescence energy transfer (LET) using double-wavelength emission (550 nm and 812 nm) upconversion nanoparticles (UCNPs) as donor, gold nanoparticles (AuNPs) as acceptor and the aptamer for S. aureus as the specific recognition and link unit. The LET process could cause luminescence quenching because of the spectral overlap between the acceptor and the donor at 550 nm. In the presence of S. aureus , S. aureus selectively combined with the aptamer, and the AuNPs left the surface of UCNPs, which weakened the quenching effect and restored the luminescence of UCNPs. Based on this, the ratio detection was realized by monitoring the change of the luminescence signal of the probe at 550 nm and taking the luminescence signal at 812 nm as the reference signal. Crucially, the probe has a fast reaction speed, with a reaction time of 25 min, and the detection of S. aureus is realized in the concentration range of 5.0 × 10 3 –3.0 × 10 5 CFU/ml, with the detection limit of 106 CFU/ml. Therefore, the ratio probe has great potential for detecting of S. aureus in food because of its high sensitivity, fast speed and good selectivity.
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IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Song Xie,Haotian Wu,Xiaozhan Yang
A microfluidic electrochemical sensor is fabricated for the measurement of chemical oxygen demand (COD) in surface waters such as lakes and reservoirs. An Au interdigital electrode is used as a substrate, and a layer of Au nanoparticles (AuNPs) is deposited on the working electrode surface to form a functionalized working electrode. A polydimethylsiloxane (PDMS) microfluidic device was fabricated and integrated with the AuNPs/Au to form a PDMS-AuNPs/Au microfluidic electrochemical sensor. Water samples are transferred into and out of the sensor through a peristaltic pump and Tygon tubes, and the online COD detection was achieved using an electrochemical workstation. The linear range of the sensor is 0-400 mg/L, and the detection limit is 0.12 mg/L, which is much lower than the concentration limit of 20 mg/L specified by the Chinese national standard. In addition, five real water samples are evaluated, and the COD values are in good agreement with that of the standard method. The size of the sensor is only 30 mm×10 mm×5 mm, each measurement can be obtained within 3 minutes, and no toxic reagents are needed. In addition, the sensor has good convenience and integration, and provides a new idea for online COD detection.
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International Journal of Electrochemical Science
·
2024
·
IF 1.3
Aiping Wang,Aihua Zhou,Jing Sui
In this work, an electrochemical sensor for simultaneous detection of hydroquinone (HQ) and catechol (CC) has been developed based on nanocomposite of Au nanoparticles (AuNPs) decorated poly(3,4-ethylenedioxythiophene) (PEDOT) nano-horns. AuNPs-PEDOT nanocomposite with a novel porous structure formed by lots of interconnected horn-like PEDOT has been facially prepared via a one-pot interfacial polymerization. The top and root-end diameters of horn-like PEDOT are around10 nm and 100 nm, respectively, while AuNPs with diameter ranging from 3 to 10 nm are evenly dispersed on PEDOT. The as-synthesized AuNPs-PEDOT nanocomposite modified glassy carbon electrode (GCE) exhibits high electrocatalytic property and excellent selectivity towards the redox reaction of HQ and CC. The AuNPs-PEDOT/GCE can simultaneously detect HQ and CC with detection limits (S/N = 3) of 0.38 µM and 0.43 µM for HQ and CC respectively. Moreover, the modified electrode has excellent selectivity and is successfully applied to detect HQ and CC in real tap water sample with recovery of 95.2–104.1% and 96.2–105.6%, respectively.
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