INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2022
·Vol. 57
·期 7
·4646-4655
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IF 3.612
Ligang Yu,Yong Li,Yukun Yang,Caixia Guo,Meiping Li
Summary This study investigated the inhibitory effects of curcumin and piperine on fluorescent advanced glycation end products (fAGEs) formation in a bovine serum albumin (BSA)–fructose model. Model systems of BSA and fructose were prepared, and curcumin or piperine was added. fAGEs and BSA oxidation product (dityrosine, kynurenine and N'-formylkynurenine) contents were determined. The results showed that fAGEs content decreased with increasing concentration of curcumin and piperine ( P < 0.05). Addition of curcumin and piperine at 160 µg mL −1 could inhibit fluorescent AGEs by 100% and 93% respectively. Dityrosine and N'-formylkynurenine contents decreased as curcumin and piperine concentration increased ( P < 0.05). Furthermore, the result of principal component analysis indicated that curcumin and piperine markedly impeded BSA oxidation, resulting in a lower level of fAGEs in model systems. Therefore, adding curcumin and piperine may facilitate reduced fAGEs levels in BSA–fructose model.
研究领域:
生物化学
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药理学
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食品科学
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产品关联
DOI
高影响因子
Biomass Conversion and Biorefinery
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2024
·
IF 3.5
Wang Xuchong,Wei Yuhe,Sun Xiaoping,Yuan Juan,Chen Haoqiu,Sun Yanfen,Luo Lianxin
Pretreatment is the key step in enzymatic hydrolysis of bamboo wood. Green liquor pretreatment is a promising method to improve enzymatic hydrolysis and saccharification efficiency by changing the physical and chemical properties of lignocellulose surface. At present, there is little work to quantitatively analyze the enzymatic saccharification rate of bamboo lignocellulose by two-stage pretreatment. In this study, a two-stage pretreatment method combining hot water and green liquid phase was mainly used, and the bamboo was treated with Bovine serum albumin (BSA) and Tween80 (TW) chemical additives. After that, the treated samples were hydrolyzed by cellulase, and the effects of pretreatment on the chemical composition and physical properties of bamboo and the influencing factors of chemical additives on the enzymatic hydrolysis efficiency of bamboo were explored. The results showed the two pretreatment stages of hot water and green liquid to have an evident effect on the enzymatic hydrolysis of bamboo. The two-stage pretreatment with hot water and green liquid (14% total alkali) resulted in an 85.51% yield of total sugar hydrolyzed by the enzyme. The addition of BSA and Tween80 improved the efficiency of the enzymatic hydrolysis of bamboo, and the synergistic effect of them could make the total sugar yield of enzymatic hydrolysis reach 99.67%. Through calculation, 65.88 g of fermentable sugar can be obtained per 100 g of bamboo fiber through enzymatic hydrolysis with cellulase. Graphical Abstract
研究领域:
生物化学
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可再生资源与环境
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2016
·
IF 3.926
Jia-Li Gu,Hong-Fei Tong,Dong-Qiang Lin
Magnetic particles modified with 5-amino-benzimidazole (ABI), a ligand for hydrophobic charge-induction chromatography, were prepared and used for antibody capture. In this study, with IgG as the model target, and bovine serum albumin (BSA) as the model impurity, the separation mechanism and process of IgG was investigated. The adsorption isotherms of IgG and BSA were measured, and the effects of pH were investigated in the range of pH 4.0–8.0. The maximum adsorption capacity of IgG on the particles was 180 mg/ml at pH 7.0, while low adsorption capacity of BSA (64 mg/ml) was found at pH 7.0, resulting in good selectivity. The protein-ligand interactions were elucidated by adding NaCl and glycerol. The results indicated the hydrophobic interactions were the main forces for IgG-ligand association. Moreover, the batch uptake and desorption experiments demonstrated the fast adsorption and desorption processes for IgG separation. The purity of IgG separated from mimetic serum could reach 98.6%, and the purity of monoclonal antibody (mAb) from a cell culture supernatant was 97.1%. Magnetic particles with hydrophobic charge-induction ligands showed a robust performance and could purify antibody directly from the complicated feedstock without clarification, which would improve the efficiency of antibody purification.
研究领域:
生物化学
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蛋白质纯化
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2018
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IF 5.738
Wangmeng Hou,Lin Wei,Li Liu,Hanying Zhao
Well-defined polymer–protein bioconjugates are widely used in therapeutics and biocatalysis. One of the challenges in the synthesis of bioconjugates is the efficient separation of the target conjugate molecules from reaction systems. In this research, surface coassembly of polymer brushes and polymer–protein bioconjugates is investigated, and it is demonstrated that the coassembly approach can be applied in the purification of polymer–protein bioconjugates. Bovine serum albumin-poly(N-isopropylacrylamide) (BSA-PNIPAM) bioconjugates were synthesized by the “grafting from” approach, and PNIPAM brushes on silica particles were prepared by the “grafting to” approach. PNIPAM brushes on silica particles are able to coassemble with BSA-PNIPAM at a temperature above the lower critical solution temperature of PNIPAM. Two-layer surface structures with collapsed PNIPAM in the inner layers and BSA in the outer layers are formed on the silica particles. The size of the silica particles and molecular weight of PNIPAM on the bioconjugates exert influences on the coassembly. The coassembly approach can be used in the purification of bioconjugates. After repeated coassembly centrifugation-release cycles, all the BSA-PNIPAM bioconjugates can be removed from the reaction solutions, and the purified bioconjugates are obtained.
研究领域:
材料科学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 5
·1482
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IF 4.927
Yacong An,Xundou Li,Fengjiao Yao,Jinhong Duan,Xian-Da Yang
The PD-1/PD-L1 pathway blockade can generate a good clinical response by reducing immunosuppression and provoking durable antitumor immunity. In addition to antibodies, aptamers can also block the interaction between PD-1 and PD-L1. For the in vivo application, however, free aptamers are usually too small in size and quickly removed from blood via glomerular filtration. To avoid renal clearance of aptamer, we conjugated the PD-L1 aptamer to albumin to form a larger complex (BSA-Apt) and evaluated whether BSA-Apt would enhance the in vivo antitumor efficacy. The PD-L1 aptamer was thiol-modified and conjugated to the amino group of BSA via a SMCC linker. The average size of BSA-Apt was 11.65 nm, which was above the threshold for renal clearance. Functionally, BSA-Apt retained the capability of the PD-L1 aptamer to bind with PDL1-expressing tumor cells. Moreover, both the free aptamer and BSA-Apt augmented the PBMC-induced antitumor cytotoxicity in vitro. Furthermore, BSA-Apt generated a significantly stronger antitumor efficacy than the free PD-L1 aptamer in vivo without raising systemic toxicity. The results indicate that conjugating the PD-L1 aptamer to albumin may serve as a promising strategy to improve the in vivo functionality of the aptamer and that BSA-Apt may have application potential in cancer immunotherapy.Keywords:PD-L1;aptamer;albumin;BSA-Apt;cancer;immunotherapy
研究领域:
肿瘤学
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免疫学
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产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
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2021
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IF 5.455
Hui Chen,Yan Zhang,Xiangying Xin,Nannan Feng,Deqiao Wu,Junwei Zhang,Bing Fang,Nan Zhang
In order to improve the degradation rate of silica framework and the targeted release ability of tumor drugs, this paper used a one-pot synthesis method to synthesize a redox with a silsesquioxane framework disulfide bridge and a particle size of about 150 nm. The triggered biodegradable organic-inorganic hybrid nanocapsule is a new and unique hollow mesoporous nanocapsule that is sequentially coupled with bovine serum albumin (BSA) and folic acid (FA) for the targeted release of adriamycin. As a “control gate”, BSA can prevent the premature release of drugs until the BSA coating “opens the door” in response to Glutathione(GSH) to achieve drug release. FA specifically recognizes the folate receptor on the tumor surface to achieve tumor targeting. Under acidic PH and GSH, it can achieve accelerated and complete release of drugs, and effectively consume intracellular GSH, help break the redox barrier in hypoxic cells, cause oxidative stress, and effectively inhibit tumor growth.
研究领域:
纳米技术
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药学
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癌症研究
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材料科学
✓ 已验证
DOI
高影响因子
Journal of Functional Biomaterials
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2025
·Vol. 16
·期 2
·69
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IF 5.2
Yuanyuan Qu,Jinlong Li,Xin Jia,Lijun Yin
Hydrogel network structures play a crucial role in determining mechanical properties and have broad applications in biomedical and industrial fields. Therefore, their rational design is essential. Herein, we developed a Schiff base-crosslinked hydrogel through the reaction of Tetra-armed polyethylene glycol with aldehyde end groups (Tetra-PEG-CHO) and bovine serum albumin (BSA) under alkaline conditions. In addition, the Tetra-PEG-BSA hydrogel showed a rapid gelation time of around 11 s, much faster than that of the GLU-BSA, HT-BSA, and GDL-BSA hydrogels. It had high optical transmittance (92.92% at 600 nm) and swelling ratios superior to the other gels in different solutions, maintaining structural integrity even in denaturing environments such as guanidine hydrochloride and SDS. Mechanical tests showed superior strain at break (84.12 ± 0.76%), rupture stress (28.64 ± 1.21 kPa), and energy dissipation ability (468.0 ± 34.9 kJ·m−3), surpassing all control group hydrogels. MTT cytotoxicity assays indicated that cell viability remained >80% at lower concentrations, confirming excellent biocompatibility. These findings suggest that Tetra-PEG-BSA hydrogels may serve as effective materials for drug delivery, tissue engineering, and 3D printing.
研究领域:
生物材料学
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生物医药工程
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高分子化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Shengyu Li,Lihong Gong,Xijin Wu,Xianli Liu,Ningning Bai,Yingxue Guo,Xia Liu,Hong Zhang,Huiying Fu,Qiyang Shou
Currently, protein-based hydrogels are widely applied in soft materials, tissue engineering and implantable scaffolds owing to their excellent biocompatibility, and degradability. However, most protein-based hydrogels are soft brittle. In this study, a ductile and mechanically enhanced bovine serum albumin (BSA) hydrogel is fabricated by soaking the a 1-(3-dimethylaminopropyl)-3ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) induced BSA hydrogel in (NH 4 ) 2 SO 4 solution. An EDC/NHS coupling reaction induce protein coupling reactions that cause the BSA skeleton to resemble architectural load-bearing walls, protecting the integrity of the hydrogel and preventing collapse. The effects of the BSA and (NH 4 ) 2 SO 4 concentrations on the hydrogel mechanics are evaluated, and the possible strengthening mechanism is discussed. Besides, the highly kosmotropic ions greatly enhance the hydrophobic interaction within BSA gels and dehydration effect and their mechanical properties were significantly enhanced. The various mechanical properties of hydrogels can be regulated over a large window by soaking hydrogels into various ions. And most of them can be washed away, maintaining high biocompatibility of the protein. Importantly, the protein hydrogels prepared by this strategy could also be modified as strain sensors. In a word, this work demonstrates a new, universal method to provide multi-functional, biocompatible, strength enhanced and regulable mechanical pure protein hydrogel, combining the Hofmeister effect with -NH 2 /-COOH association groups.
研究领域:
生物材料学
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组织工程学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2020
·Vol. 18
·期 6
·315
·
IF 4.073
Qinying Yan,Jiaqi Weng,Xieqi Wu,Weiwei Wang,Qingliang Yang,Fangyuan Guo,Danjun Wu,Ying Song,Fan Chen,Gensheng Yang
Chitosan nanoparticles (CS-NPs) are under increasing investigation for the delivery of therapeutic proteins, such as vaccines, interferons, and biologics. A large number of studies have been taken on the characteristics of CS-NPs, and very few of these studies have focused on the microstructure of protein-loaded NPs. In this study, we prepared the CS-NPs by an ionic gelation method, and bovine serum albumin (BSA) was used as a model protein. Dynamic high pressure microfluidization (DHPM) was utilized to post-treat the nanoparticles so as to improve the uniformity, repeatability and controllability. The BSA-loaded NPs were then characterized for particle size,Zetapotential, morphology, encapsulation efficiency (EE), loading capacity (LC), and subsequent release kinetics. To improve the long-term stability of NPs, trehalose, glucose, sucrose, and mannitol were selected respectively to investigate the performance as a cryoprotectant. Furthermore, trehalose was used to obtain re-dispersible lyophilized NPs that can significantly reduce the dosage of cryoprotectants. Multiple spectroscopic techniques were used to characterize BSA-loaded NPs, in order to explain the release process of the NPs in vitro. The experimental results indicated that CS and Tripolyphosphate pentasodium (TPP) spontaneously formed the basic skeleton of the NPs through electrostatic interactions. BSA was incorporated in the basic skeleton, adsorbed on the surface of the NPs (some of which were inlaid on the NPs), without any change in structure and function. The release profiles of the NPs showed high consistency with the multispectral results.Keywords:characteristics;cryoprotection evaluation;dynamic high pressure microfluidization;chitosan nanoparticles
研究领域:
生物材料学
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药物递送系统
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 4
·1637-1644
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IF 3.269
Zengming Yang,Hengchang Ma,Zijie Jin,Haiying Cao,Lei Lei,Yucheng Ma,Ziqiang Lei
It is desirable to engineer bio-compatible construction materials and cargo release vehicles with efficient cargo loading capability and controlled drug delivery ability. Herein, an inorganic–organic hybrid composite DNDT&SiO2–NH2@BSA was established using two steps. Technologically, the fluorescent DNDT was grafted on the particles surface via solid phase synthesis, which was able to report the occurrence of interactions between the silica cores and proteins of bovine serum albumin (BSA) by real-time optical change analysis. It should be noted that the construction method derived from this study can be transposed easily to other surface modification procedures. Interestingly, DNDT&SiO2–NH2@BSA could be dispersed into an aqueous phase very well, leading to a homogeneous distribution of the drug over the matrix with a more efficient manner. Furthermore, due to the temperature-dependent α-helix content changes, the encapsulators of BSA can capture the drugs at low temperature and release them with elevating temperature. In particular, a desirable controlled release is realized at 37 °C.
研究领域:
材料科学
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生物医学工程
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药学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Yaqing Chang,Zhe Zhang,Jinhui Hao,Wenshu Yang,Jilin Tang
Rapid and sensitive detection of Ag + in aquatic ecosystems is of great significance for public health and environmental security. In this report, a simple and feasible colorimetric method for the detection of Ag + at an ultralow concentration is developed. Bovine serum albumin stabilized gold nanoclusters (BSA@AuNCs) possess the peroxidase-like activity that could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 . Once Ag + is introduced, Ag + selectively reacts with Au° through redox reaction, which induces an apparent inhibition of the peroxidase-like activity of BSA@AuNCs. On the basis of this effect, a colorimetric Ag + sensor is established. The high specificity of the Ag–Au interaction provides excellent selectivity over the interfering metal ions. The lowest detectable concentration for Ag + is 0.204 μM. And this method could be applied for the rapid analysis of Ag + in lake water with satisfactory results. These advantages demonstrate that the proposed method is a promising candidate for the detection of Ag + in water samples.
研究领域:
环境科学
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分析化学
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纳米生物学
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产品关联
DOI
高影响因子
Biomolecules
·
2023
·Vol. 13
·期 3
·496
·
IF 5.5
Yingyuan Zhao,Zhaoxuan Wang,Shuxian Liu,Shiying Xie,Yinchun Xie,Ruifang Li,Hiroaki Oda
Astaxanthin is a carotenoid with excellent antioxidant activity. However, this small lipid-soluble molecule is insoluble in water and has low stability. Although this situation can be improved when astaxanthin is prepared as a nanosuspension, the aqueous form is still not as convenient and safe as the dry powder form for storage, transport, and use. The lyophilization process provides better protection for thermosensitive materials, but this leads to collapse and agglomeration between nanoparticles. To improve this situation, appropriate lyophilization protectants are needed to offer support between the nanoparticles, such as sugars, amino acids, and hydroxy alcohols. The purpose of this work is to screen lyophilization protectants by single-factor experiments and response surface optimization experiments and then explore the optimal ratio of compound lyophilization protectants, and finally, make excellent astaxanthin/BSA/chitosan nanosuspension (ABC-NPs) lyophilized powder. The work shows that the optimal ratio of the compounding lyophilization protectant is 0.46% oligomeric mannose, 0.44% maltose, and 0.05% sorbitol (w/v). The ABC-NPs lyophilized powder prepared under the above conditions had a re-soluble particle size of 472 nm, with a ratio of 1.32 to the particle size of the sample before lyophilization. The lyophilized powder was all in the form of a pink layer. The sample was fluffy and dissolved entirely within 10 s by shaking with water. Consequently, it is expected to solve the problem of inconvenient storage and transportation of aqueous drugs and to expand the application of nanomedicine powders and tablets.Keywords:lyophilization;astaxanthin nanoparticles;freeze-drying protectant
研究领域:
药学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）