Experimental and Therapeutic Medicine
·
2023
·Vol. 25
·期 2
·1-10
·
IF 2.7
Qing Chen,Shaojin Zhu,Nanlin Jiao,Ziyu Zhang,Guangjian Gao,Wenqiang Zheng,Gang Feng,Wenzheng Han
The early diagnosis of lung cancer is closely associated with the decline of mortality. A panel consisting of seven lung cancer‑related autoantibodies (7‑AABs) has been shown to be a reliable and specific indicator for the early detection of lung cancer, with a specificity of ~90% and a positive predictive value of ~85%. However, its low sensitivity and negative predictive value limit its wide application. To improve its diagnostic value, the diagnostic efficiencies of 7‑AABs in combination with non‑specific tumor markers were retrospectively investigated for the detection of early‑stage lung cancer. A total of 217 patients with small lung nodules who presented with ground‑glass opacity or solid nodules as well as 30 healthy controls were studied. The concentrations of 7‑AABs and heat shock protein 90a (HSP90a) were assessed using ELISA. Automated flow fluorescence immune analysis was used for the assessment of CEA, CYFRA21‑1, CA199 and CA125 levels. The results showed that 7‑AABs + HSP90a possessed a remarkably improved diagnostic efficiency for patients with small pulmonary nodules or for patients with lung nodules of different types, which suggested that 7‑AABs in combination with HSP90a could have a high clinical value for the improvement of the diagnostic efficiency of early‑stage lung cancer.
研究领域:
肿瘤学
·
医学诊断学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 63
·51330-51336
·
IF 3.84
Tao Wang,Lei Guo,Chong Lei,Yong Zhou
Herein, a giant magnetoimpedance (GMI) sensor combined with the sandwich immunoassay was used for ultrasensitive detection of carcinoembryonic antigens (CEA). Oblong-shaped Au films were enclosed with SU-8 photoresists to form several microcavities that were treated with different concentrations of CEA. The quantification of low-concentration CEA (1 pg mL−1–10 ng mL−1) was achieved by using one single GMI sensor, demonstrating a good linear relationship between the GMI response and the CEA concentration. The lower detection limit for CEA was found to be as low as 1 pg mL−1, which provides extreme sensitivity. We observed that high-concentration CEA (>10 ng mL−1) can result in a cluster of Dynabeads, thereby reducing the target signals since the adjacent magnetic dipole fields canceled each other out. Target specificity of the GMI-based magnetic immunoassay has also been verified by testing nonspecific targets. The results obtained here suggest that the GMI sensor could serve as an ultrasensitive immunosensor for quantitative determination of biomarkers.
研究领域:
生物传感器
·
医学诊断
·
材料科学
·
免疫学
✓ 已验证
DOI
高影响因子
Journal of Extracellular Vesicles
·
2023
·Vol. 12
·期 1
·12300
·
IF 16
Zhaowei Zhang,Xuehui Liu,Xiaoqing Yang,Ying Jiang,Ang Li,Jiying Cong,Yuwei Li,Qinjian Xie,Chen Xu,Dingbin Liu
Colorectal cancer (CRC) is one of the most common malignancies that is usually detected late in the clinic. The currently available diagnostic tools for CRC are either invasive or insensitive to early lesions due to the dearth of reliable biomarkers. In this study, we discovered that the extracellular vesicles (EVs) in the faeces of CRC patients can act as a potent biomarker for the non-invasive diagnosis and prognosis of CRC. This finding is based on the identification of two transmembrane proteins—CD147 and A33—on faeces-derived EVs (fEVs) that are intrinsically associated with CRC. The detection results show that the levels of CD147 and A33 on fEVs were upregulated in the CRC patients ( n = 48), dramatically distinguishing them from the healthy donors ( n = 16). The CD147/A33-enriched EVs offer a clinical sensitivity of 89%, much higher than that (40%) of carcinoembryonic antigen (CEA), a clinically-established serum biomarker for CRC diagnosis. In addition, the analysis of longitudinal faeces samples ( n = 29) demonstrated that the CD147/A33-enriched fEVs can be utilized to track the prognosis of CRC. Due to the high compliance of faeces-based detection, the CD147/A33-enriched fEVs could serve as new-generation CRC biomarkers for large-scale, non-invasive CRC screening as well as real-time monitoring of patient outcomes during clinical interventions.
研究领域:
肿瘤学
·
胃肠病学
·
分子生物学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2023
·Vol. 52
·期 8
·5662-5669
·
IF 2.1
Yang Xiande,Chen Ying,Qin Yijin,Wen Lizhen
Carcinoembryonic antigen (CEA) is an important tumor marker, which is identified as a critical marker in some tumors. Thus it is of great practical value to seek an effective and sensitive method for CEA detection. Scholars found that the specific modification of solid-state nanochannels can construct special response characteristics. Cadmium sulfide (CdS) is an outstanding photoelectric nanomaterial due to its excellent optical properties. Inspired by the potential application of solid-state nanochannels and CdS, we used anodic aluminum oxide (AAO) to synthesize CdS nanoparticles (NPs) in AAO nanochannels for CEA detection. Field emission scanning electron microscopy (FESEM) and energy-dispersive spectrometry (EDS) results showed that CdS NPs were successfully self-assembled in AAO nanochannels. When we used AAO + CdS nanochannels for CEA detection, the I – V curves exhibited a broad determination range from 0.1 ng/mL to 100 ng/mL. Therefore, this method has important potential for nanomaterials self-assembled in AAO nanochannels in tumor marker detection for clinical diagnosis.
研究领域:
纳米技术
·
生物传感器
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Analytical Methods in Chemistry
·
2022
·
IF 2.594
Yanling Zhao,Yanfei Wen,Xing Hu,Bing Zhang
We proposed a colorimetric immunosensor based on g-C3N4@Fe3O4 nanocomposite-mediated transformation strategy for sensitive detection of carcinoembryonic antigen (CEA). The g-C3N4@Fe3O4 nanocomposite was synthesized and characterized by the scanning electron microscope (SEM), energy dispersive X-ray spectra (EDX), X-ray powder diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Fe3+ derived from g-C3N4@Fe3O4 nanocomposite could combine with sodium salicylate to form purple complex products. Based on this color development, the sandwich colorimetric immunoassay was built by utilizing g-C3N4@Fe3O4 nanocomposite as nanolabels on the microplate. With the increasement of CEA concentration, the purple color showed a gradient change. Under optimal conditions, the linearity range is 0.001–50 ng/mL with the detection limit of 0.35 pg/mL for CEA. More importantly, the colorimetric immunoassay has good selectivity, specificity, repeatability, and stability.
研究领域:
生物传感器
·
分析化学
·
癌症诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yongmei Wu,Gaiping Li,Lina Zou,Sheng Lei,Qian Yu,Baoxian Ye
Herein, we developed a high-performance electrochemical aptasensor for the detection of carcinoembryonic antigen (CEA) using highly active peptide-conjugated hemin/G-quadruplex (hGQ-peptide) and porous Pd nanoparticles (PdNPs). Of note, the hGQ-peptide showed an enhanced catalytic activity as compared to the unmodified hGQ, and thus served as efficient electrocatalyst in this biosensor. Porous PdNPs acted as supported matrixes for high immobilization of hGQ-peptide hybrids, aptamer, redox-active toluidine blue (Tb) and alcohol dehydrogenase (ADH), resulting in the formation of ADH/Tb/hGQ-peptide/aptamer/PdNPs as the proposed bioprobes. Upon the sandwich-type specific reaction between aptamer and CEA, the bioprobe was presented on the sensing interface. Highly effective signal amplification could be then achieved by the bioprobe catalyzed cascade enzymatic reaction. Compared with the original hGQ, the hGQ-peptide yielded more than 2-fold improvement in signal amplification because of the peptide-enhanced catalysis. Therefore, the designed aptasensor displayed a wide linear range from 0.0001 to 100 ng mL −1 with a lower detection limit of 20 fg mL −1 for CEA determination, presenting the better analytical performance as compared to the relevant prior biosensors. This work further expanded the application of hGQ DNAzyme in biosensor. With the good anti-interference ability and selectivity, the proposed electrochemical aptasensor held great promise for ultrasensitive detection of other proteins in clinical diagnosis.
研究领域:
生物化学
·
分析化学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Shen Liyue,Peng Tao,Dong Jiahui,Liang Zhanwei,Si Jihao,Ye Hua,Xie Jie,Yu Xiaoping,Dai Xinhua
Biomarkers screening is a benefit approach for early diagnosis of major diseases. In this study, magnetic nanoparticles (MNPs) have been utilized as labels to establish a multi-line immunochromatography (MNP-MLIC) for simultaneous detection of carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA 19–9), and alpha-fetoprotein (AFP) in a single serum sample. Under the optimal parameters, the three biomarkers can be rapidly and simultaneously qualitative screening within 15 min by naked eye. As for quantitative detection, the MNP-MLIC test strips were precisely positioned and captured by a smartphone, and signals on the test and control lines were extracted by ImageJ software. The signal ratio of test and control lines has been calculated and used to plot quantitative standard curves with the logarithmic concentration, of which the correlation coefficients are more than 0.99, and the limit of detection for CEA, CA 19–9, and AFP were 0.60 ng/mL, 1.21 U/mL, and 0.93 ng/mL, respectively. The recoveries of blank serum were 75.0 ~ 112.5% with the relative standard deviation ranging from 2.5 to 15.3%, and the specificity investigation demonstrated that the MNP-MLIC is highly specific to the three biomarkers. In conclusion, the developed MNP-MLIC offers a rapid, simple, accurate, and highly specific method for simultaneously detecting multiple biomarkers in serum samples, which provides an efficient and accurate approach for the early diagnosis of diseases. Graphical Abstract
研究领域:
医学诊断
·
纳米技术
·
生物标志物筛查
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Hongji Li,Junqing Qin,Mingji Li,Cuiping Li,Sheng Xu,Lirong Qian,Baohe Yang
A boron-doped graphene (BG)/boron-doped diamond (BDD) composite film was obtained by continuously growing BDD and BG by chemical vapor deposition. In this process, the sp 3 C C bonds in the diamond phase structure are directly converted into sp 2 C C bonds in graphene to combine the two carbon materials, while BG nanosheets are vertically grown on the exposed planes of BDD crystals. These process features impart the vertical BG (VBG)/BDD film a large specific surface area and high electrocatalytic activity, resulting in an electroactive surface area of 2.35 cm 2 . Electrodeposition can be used to load a large number of Au nanoparticles (NPs) on the VBG/BDD electrode. The Au-VBG/BDD electrode was used to construct an immunosensor capable of simultaneously quantifying carcinoembryonic antigen (CEA) and carcinoma antigen 125 (CA 125) at concentrations of 0.5–100 pg mL –1 and 0.5–100 mU mL –1 , respectively, with corresponding detection limits of 0.15 pg mL –1 and 0.09 mU mL –1 . This method is suitable for the practical detection of CEA, CA125, and other tumor markers in human body fluids, allowing early cancer diagnostics and timely treatment.
研究领域:
材料科学
·
分析化学
·
生物医学工程
·
癌症诊断
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Xingda Song,Rongbin Nie,Xiaoxia Liu,Yiping Chen,Li Yang
We proposed an innovative surface modification-mediated porous layer open tubular (PLOT) capillary, which was modified via an in situ biphasic reaction. This capillary comprised three-dimensional homogeneous and porous structures, which could increase the surface-area-to-volume ratio for antibody immobilization. The PLOT capillary was shown as an ideal immunoreaction base to enhance the sensitivity of immunoassays and shorten analysis time. By connecting two separate PLOT capillaries using a suitable sleeve tube, we can perform multiplex targets detection in the same sample. We developed a sensitive, rapid, and multiplex PLOT capillary-mediated immunosensor for the simultaneous identification of alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA) in clinical serum samples with good accuracy. The detection sensitivity of the PLOT immunosensor improved by 10-fold compared with that of bare-capillary sensor, and the whole analysis could be completed within 1 h. This work suggest that suitable surface modification strategy is an effective tool to improve the analytical performance of conventional immunoassay and our study provided a feasible, sensitive, and multi-target assay for the detection of cancer biomarkers, which would be of valuable application in clinical diagnosis.
研究领域:
分析化学
·
生物医学工程
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yue Li,Di Shi,Tiantian Liang,Jiabo Zhang,Yanhong Ni,Pengsheng Jing,Chun Mao,Runzhi Deng
The rapid and accurate detection of tumor marker carcinoembryonic antigen (CEA) is of great significance for the early diagnosis of cancer. Here, we design an electrochemical detection platform based on zwitterionic surface modification, nanomotor and magnetic separation technology. Specifically, magnesium-based micromotors (magnesium/ferroferric oxide/poly(carboxybetaine methacrylate)/ carcinoembryonic antigen antibody, Mg/Fe 3 O 4 /PCBMA/anti-C) were firstly prepared using asymmetric surface modification technology, which could react with water to form active motion to promote the capture of the target detection substance CEA. Furthermore, after the Mg-based micromotor was completely consumed in the test solution, a self-made funnel device was used to collect the magnetic nanoparticles that captured CEA onto the surface of the magnetic electrode. Then, the electrochemical testing was performed. When CEA bound with anti-CEA, the resulting immune complex hindered the electrochemical probe Fe(CN)₆³⁻/⁴⁻ from reaching the electrode surface, thereby causing a decrease in the electrochemical signal. By measuring the change in the electrochemical signal, the concentration of CEA could be quantitatively determined. The experimental results indicate that Mg-based micromotors display good blood compatibility and could actively capture CEA in whole blood. This detection platform was very sensitive for CEA detection, with a detection range from 0.001 ng mL -1 to 100 ng mL -1 and a detection limit of 2.58×10 -4 ng mL -1 . This multi-technology composite application of whole blood antigen detection is expected to provide a new technological platform for early diagnosis of cancer.
研究领域:
生物医学工程
·
纳米技术
·
生物传感器
·
电化学
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2022
·
IF 3.191
Ping Wang,Yaoyao Xie,Huikai Ma,Jiancheng Liu,Chenxiang Liu,Wei Feng,Shoumin Xi
A novel ratiometric electrochemical aptasensor was proposed for carcinoembryonic antigen (CEA) detection based on exonuclease III (Exo III)-assisted recycling and rolling circle amplification (RCA) strategies. A thiolated ferrocene-labeled hairpin probe 2 (Fc-HP2) was fixed on the gold nanoparticles (AuNPs)-modified gold electrode (AuE) surface through Au–S bonds. The presence of CEA led to the release of trigger, which hybridized with the 3′-protruding of hairpin probe 1 (HP1) and triggered the Exo III cleavage reaction, accompanied by the releasing of trigger and generation of new DNA fragment which was used for the successive hybridization with Fc-HP2. After the Exo III cleavage process, the remaining Fc-HP2 fragments hybridized as primers with the RCA template to initiate the RCA process, and long single-stranded polynucleotides were produced for methylene blue (MB) binding. Such changes resulted in the signal of Fc (I Fc ) decreased and that of MB (I MB ) increased, achieving a linear relationship between I MB /I Fc and logarithm of CEA concentrations ranging from 1.0 pg mL −1 to 100.0 ng mL −1 with a detection limit of 0.59 pg mL −1 . Additionally, the developed aptasensor had been successfully applied to detect CEA in human serum samples. Therefore, the proposed strategy might provide a new platform for clinical detections of CEA.
研究领域:
生物化学
·
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 8
·2797-2802
·
IF 4.019
Piaopiao Chen,Ke Huang,Rui Dai,Erica Sawyer,Ke Sun,Binwu Ying,Xiawei Wei,Jia Geng
Nowadays, label-free atomic spectrometric bioassays are attracting great research interest because of their advantages of low cost, simple design and operation, etc. Herein, a novel and simple chemical vapor generation-atomic fluorescence spectrometry (CVG-AFS)/inductively coupled plasma-mass spectrometry (ICP-MS) label-free detection method is presented for highly sensitive and selective assay of DNA and proteins. This work mainly combined a phenomenon that CdTe quantum dots (QDs) can be used to selectively differentiate free Hg2+ and the T–Hg2+–T complex, with the use of simple membrane filtration separation to improve the performance of the label-free bioassay methods. Upon hybridization with the DNA/protein (carcinoembryonic antigen, CEA) target, the T–Hg2+–T hairpin structure was opened and Hg2+ was released; this initiated the cation exchange reaction between Hg2+ and CdTe QDs which released Cd2+ simultaneously. Subsequently, the free Cd2+ was separated by the filtration membrane without separating the CdTe QDs, which could then be separated from the sample matrices for the CVG-AFS/ICP-MS assay. Under the optimal conditions, this method possessed high sensitivity for DNA and CEA determination with limits of detection (LODs) of 0.2 nM and 0.2 ng mL−1, and linear dynamic ranges of 1–160 nM and 0.5–20 ng mL−1, respectively, and exhibited excellent DNA sequence specificity and protein selectivity. This method preserves the advantages of the label-free atomic spectrometric bioassay, and combined with the selective cation exchange reaction and simple filtration separation to improve the performance.
研究领域:
分析化学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）