COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Qun Liu,Ning Tang,Luyao Ding,Zengyu Sun,Wenju Jiang,Benke Cui,Haozhuo Zhang,Hui Yang,Baoqi Ding,Yuan Wang,Xiuzhi Wang,Chunguang Ren
Preventing infection, improving oxygenation and enabling automated coverage of irregularly shaped wounds are the critical factors for ensuring the efficacy of wound dressings. Traditional wound dressings often fail to meet the diverse and complex demands throughout the wound healing process due to insufficient mechanical strength and limited functionality. Herein, we constructed a multifunctional hydrogel dressing (CPA) utilizing polyvinyl alcohol (PVA) and collagen (COL) as matrix, incorporated Chlorella and ampicillin (AMP) to confer the hydrogel with oxygen-supplying and antibacterial capabilities. Owing to the existence of borate ester bond, CPA hydrogel demonstrated superior mechanical properties, remarkable self-healing capabilities, and shape adaptability. The hydrogel exhibited efficient hemostatic capability, favorable hemocompatibility and cell compatibility. In vivo evaluation further demonstrated the hydrogel’s efficacy in significantly promoting collagen deposition, thereby accelerating wound healing. These findings collectively highlight the CPA hydrogel's efficacy and underscore its potential for application in the management of irregular, hypoxic, and infected wounds. Download: Download high-res image (167KB) Download: Download full-size image
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高影响因子
Journal of Environmental Chemical Engineering
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2026
·Vol. 14
·期 5
·124002
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IF 7.5
Mihebai Yilimulati,Dongyuan Zhang,Jieyu Wang,Zaimire Abudushalamu,Wentao Zhang,Nuzahat Habibul
The ecological effects of antibiotics at environmentally relevant low concentrations have attracted considerable attention, but how they regulate algal growth and influence bloom risks through selective, dose-dependent, and algae-specific mechanisms remains poorly understood. Moreover, most conventional toxicological studies have used high concentrations at the mg/L level, which are orders of magnitude higher than actual environmental concentrations (ranging from ng/L to μg/L). To address this gap, the present study systematically compared the selective effects and metabolic mechanisms of norfloxacin on the cyanobacterium Microcystis aeruginosa and the green alga Chlorella vulgaris under environmentally relevant concentrations. Low NOR concentrations (0.01–0.5 μg/L) stimulated the growth of Microcystis aeruginosa , increased its photosynthetic pigment content, enhanced photosystem activity, and activated metabolic pathways related to nucleotide and amino acid synthesis. Interestingly, higher NOR concentrations (5–100 μg/L) suppressed photosynthesis, induced oxidative stress, promoted the release of extracellular organic matter, and markedly disrupted central energy metabolism, including carbon fixation, glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle. In contrast, Chlorella vulgaris exhibited greater tolerance, as evidenced by maintained photosynthetic homeostasis, moderate metabolic reorganization, and no significant fluctuation in cell abundance. These findings indicate that environmentally relevant concentrations of norfloxacin may selectively stimulate the growth of the cyanobacterium Microcystis aeruginosa through a “low-dose promotion, high-dose inhibition” pattern mediated by key metabolic pathways. The differential algae-specific responses suggest that norfloxacin pollution could potentially increase the risk of cyanobacterial blooms, although this possibility remains to be validated through co-culture or field studies.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Shumin Qin,Bixiang Nie,Fu Wei,Yan Lin,Lang Jiang,Qian Zhang
The symbiotic system of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria and Chlorella shows significant nitrogen and phosphorus removal in biogas slurry treatment. It uses quorum sensing signaling molecules to form symbiotic biofilms, significantly boosting nitrogen and phosphorus removal efficiency. This study systematically explored the effect of exogenous N-octanoyl-DL-homoserine lactone (C8-HSL) concentrations on pollutant removal efficiency, extracellular polymeric substance (EPS) production, and microbial dynamics in the HN-AD bacteria- Chlorella symbiotic system. Results showed that the pollutant removal of the symbiotic system is maximized when the C8-HSL concentration is 0.8 μM. At concentrations below 0.8 μM, the system significantly enhances pollutant removal through increased EPS secretion. However, when the concentration exceeds 0.8 μM, both EPS production and pollutant removal are inhibited. Metagenomic analysis shows that C8-HSL at 0.8 μM can enrich key bacterial genera such as Halomonas and Sphingobium . By regulating key genes like narGHI , nirKS , phnX , and ppa , it adjusts their metabolic pathways, thereby influencing nitrogen and phosphorus removal. This highlights the crucial role of C8-HSL concentration in pollutant removal within HN-AD- Chlorella symbiotic systems.
研究领域:
环境微生物学
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水处理技术
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生物修复
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分子生态学
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环境工程
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微藻生物技术
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信号分子调控
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Can Zhang,Shiqi Liu,Yuqing Cao,Weidong Zhang,Yuchu He,Wenkang Tu,Weili Xue,Dawei Gao,Xuwu Zhang
The hypoxic tumor microenvironment (TME) promotes tumor progression and therapeutic resistance primarily through the activation of hypoxia-inducible factor-α (HIF-α). However, current oxygen-sensitizing strategies are hindered by low delivery efficiency, transient efficacy, and severe adverse effects. In this study, we developed a composite system (C@W) comprising Chlorella pyrenoidosa (CHL) and tungsten disulfide (WS₂). This platform synergistically integrates the photosynthetic oxygen generation capacity of CHL with the photocatalytic functionality of WS₂, enabling multimodal antitumor efficacy through hypoxic TME remodeling, photocatalytic therapy, and immune activation. Upon irradiation with 660 nm near-infrared (NIR) light, CHL continuously released oxygen via in situ photosynthesis, thereby suppressing HIF-α expression and alleviating TME hypoxia. Concurrently, WS₂ efficiently catalyzed the conversion of molecular oxygen into cytotoxic singlet oxygen ( 1 O₂). Experimental results demonstrated that this system induced immunogenic cell death (ICD) in tumor cells, promoted dendritic cell maturation via damage-associated molecular pattern (DAMP) release, facilitated M2-to-M1 macrophage polarization, and significantly enhanced tumor-infiltrating CD8 + T cell populations. This bio-inorganic hybrid platform addresses the spatiotemporal limitations of conventional oxygen-enhancing therapies by establishing a dual-engine oxygen self-supply system (photosynthesis–photocatalysis). It synergistically alleviates the immunosuppressive TME while integrating microenvironment regulation with immune activation, thereby offering a novel therapeutic paradigm for solid tumor treatment.
研究领域:
肿瘤学
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纳米医学
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光动力治疗
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肿瘤微环境
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免疫治疗
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生物杂化系统
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光合作用生物学
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PubMed
高影响因子
CHEMOSPHERE
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2024
·
IF 8.1
Jingjing Chang,Peiling Wei,Meng Tian,Ying Zou,Shenghua Zhang
Screening for sensitive toxicological indicators and understanding algal tolerance to pharmaceutical contaminants (PhCs) are essential for assessing PhCs risk and their removal by microalgae . Carbamazepine (CBZ) showed adverse effects on microalgae , but the specific toxicity mechanisms on the most sensitive algal photosynthetic system (PS) remain limited. This study delved into the impact of CBZ exposure on the growth, cell viability , pigment content, and PS of Chlorella vulgaris . The findings revealed a notable inhibition of C. vulgaris growth by CBZ, with an IC 50 value of 27.2 mg/L at 96 h. CBZ exposure induced algal membrane damage and cell viability . Intriguingly, CBZ drastically diminished intracellular pigment levels, notably showing "low promotion and high inhibition" of chlorophyll b (Chl b) by 72 h. Moreover, the study identified a decreased number of active reaction centers (RCs) within algal PSII alongside inhibited electron transport from Q A to Q B on the PSII receptor side, leading to PSII disruption. As an adaptive response to CBZ stress, C. vulgaris stimulated its Chl b synthesis, increased non-photochemical quenching (NPQ), and adapted its tolerance to bright light. Additionally, the alga attempted to compensate for the CBZ-induced reduction in electron transfer efficiency at the PSII receptor side and light energy utilization by increasing its electron transfer from downstream. Principal component analysis (PCA) further verified that the parameters on non-photochemical dissipation, electron transport, and integrative performance were the most sensitive algal toxicological indicators for CBZ exposure, and algal PS has energy protection capability through negative feedback regulation. However, prolonged exposure to high doses of CBZ will eventually result in permanent damage to the algal PS. Hence, attention should be paid to the concentration of CBZ in the effluent and the exposure time, while methods to mitigate algal photodamage should be appropriately sought for algal treatment of dense effluents.
研究领域:
环境科学
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毒理学
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PubMed
高影响因子
MARINE ENVIRONMENTAL RESEARCH
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2022
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IF 3.737
Wenjing Wang,Yanqing Sheng
Decabromodiphenyl ethane is a novel brominated flame retardant , that has always been dissolved in organic solvents to explore its activities on aquatic organisms. In this study, the influences of decabromodiphenyl ethane on the microalga Chlorella sorokiniana ( C. sorokiniana ) were studied, and three microalgae treatments, including decabromodiphenyl ethane dissolved in dimethyl sulfoxide solvent (DBDPE treatment), dimethyl sulfoxide alone (control II) or untreated (control I) were used in the experiment, respectively. The results showed that the growth of C. sorokiniana was remarkably enhanced in the DBDPE treatment compared with the control I and II groups. Conjoint analysis of transcriptomics and quantitative proteome displayed that the upregulated differentially expressed genes and proteins of DBDPE:control I were enriched in 6 pathways, and downregulated genes/proteins of DBDPE:control I were enriched in 3 pathways. The upregulated differentially expressed genes and proteins of DBDPE:control II were enriched in 4 pathways, and downregulated genes/proteins of DBDPE:control II were enriched in 6 pathways. In addition, decabromodiphenyl ethane changed the fatty acid concentration in C. sorokiniana cells. The activities of superoxide dismutase were enhanced when C. sorokiniana were treated by decabromodiphenyl ethane. The data highlighted that the mRNA and protein expression relating to the fatty acid production, of C. sorokiniana were significantly affected by decabromodiphenyl ethane, and decabromodiphenyl ethane pollution changed the physiological metabolism of microalgae and had harmful effects on natural environments.
研究领域:
环境科学
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微生物学
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分子生物学
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毒理学
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PubMed
高影响因子
Innovative Food Science & Emerging Technologies
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2026
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IF 6.9
Chen Zhang,Mengqi Nong,Yu Luo,Marco Marcucci,Yihuan Li,Zexin Lin
Chlorella pyrenoidosa is a protein-rich microalga (> 40% dry weight), and its protein extraction is challenging due to its multilayered cell wall. In this study, the protein extraction efficiency and structural disruption of high-pressure disruption (HPD), alkaline, ultrasonication, and enzymatic methods were compared. At 40 kpsi, HPD achieved 88% protein yield—outperforming alkaline (70%), ultrasonication (64%), and enzymatic (34%) methods, without evident low-molecular-weight fragmentation (based on SDS-PAGE), and maintaining a biological value of 70.1. Instantaneous pressurization fragmented cells into 0.1–10 μm particles, releasing both intracellular proteins and cell wall components. Monosaccharide analysis indicated the concurrent release of structural sugars, with HPD extracts containing 191 g·kg −1 total monosaccharides (4× aqueous extracts), including cell wall-specific muramic acid (14.7 g·kg −1 ) and N -acetylglucosamine (18.4 g·kg −1 ), alongside intracellular glucose and galactose. These findings are consistent with extensive physical disruption of the multilayered cell wall and enhanced intracellular protein release. In contrast, alkaline treatment degraded the outer sheath ( N -acetylglucosamine: 17.4 g·kg −1 ) but resulted in protein aggregation, while ultrasonication created surface pores and released protein content. Enzymatic methods (e.g., Viscozyme® L) selectively hydrolyzed surface polysaccharides without penetrating deeper layers. Scanning electron microscopy and particle size analysis confirmed extensive structural disintegration after HPD treatment. Overall, these results demonstrate the effectiveness of HPD under the tested conditions for promoting protein extraction from C. pyrenoidosa . The potential integration of mechanical disruption and enzymatic hydrolysis warrants further investigation to evaluate possible complementary effects and process efficiency.
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高影响因子
Journal of Environmental Chemical Engineering
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2021
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IF 5.909
Deyong Che,Liye Wang,Hongpeng Liu,Baizhong Sun,Shuai Guo
Biomass pyrolysis has attracted attention; however, its development has been restricted by the formation of NOx precursors, which result mainly from protein pyrolysis. In addition, the effect of lipids on this process is not clear. We extracted proteins and lipids from chlorella and sewage sludge to investigate the thermal decomposition characteristics, thermodynamic properties, kinetic parameters, and reaction mechanisms of protein with or without lipids. Two model-free methods Flynn-Wall-Ozawa (FWO) and Friedman (FM) were used to calculate the kinetic energy. Between 150 and 600 ℃, the average activation energy (E) of sludge protein was 73.63 kJ/mol (FM) or 72.53 kJ/mol (FWO), whereas with lipids, it was 129.19 kJ/mol (FM) or 124.55 kJ/mol (FWO), indicating that lipids restrict the pyrolysis of sludge protein. There was no significant difference between E of chlorella protein pyrolysis with or without lipids. Moreover, the proteins in sludge and chlorella released less NH 3 under the influence of lipids; however, the formation of HCN was affected slightly. The results of this study provide a reliable reference for the regulation of protein pyrolysis, considering the production of NOx precursors.
研究领域:
环境科学
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生物能工程
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2019
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IF 5.589
Hongwei Luo,Yahui Xiang,Dongqin He,Yu Li,Yaoyao Zhao,Shuo Wang,Xiangliang Pan
Chemical additives leaching from microplastics and their effects on physiology of microalgae are of environmental significance. So far, these issues remain unclear. Here, the leaching behavior of fluorescent additives from polyurethane sponge microplastics in simulated (acidic, saline, and basic water) and natural waters (river, lake, wetland, and sea water) was investigated. Release amount of additives increased with increasing solution pH and leaching time. The maximum release amount was reached at the leaching time of 12–24 h and the 3,3′-diaminobenzidine-like substances were identified in the leachate. The leached concentrations of fluorescent additives in simulated and natural waters followed the order of basic water > saline water > seawater > West Lake > River > Wetland. Effects of leachate and microplastics on growth and photosynthesis of Chlorella vulgaris were further evaluated. The maximum quantum efficiency of photosystem II (F v /F m ) decreased with increasing leachate concentrations. Only high content (1.6 g L −1 ) of microplastics exerted significant inhibitory influence on cell photosynthesis when microalgae were exposed to microplastics alone. Retention of algal cells inside the porous sponge microplastics did not change their photosynthetic efficiency. These findings indicate that leaching process of additives from microplastics depends mainly on water environments and the leached chemicals may pose ecological risks to aquatic organisms.
研究领域:
环境科学
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生态毒理学
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PubMed
高影响因子
AQUATIC TOXICOLOGY
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2019
·
IF 3.794
Dongdong Fu,Qiongjie Zhang,Zhengquan Fan,Huaiyuan Qi,Zezheng Wang,Licheng Peng
Microplastics (MPs) could pose potential risks to microalgae, the primary producer of marine ecosystems. Currently, few studies focus on the interaction of aged MPs with other pollutants and their toxic effects to microalgae. Therefore, the present study aimed to investigate i) the aging of microplastics polyvinyl chloride (mPVC) in simulated seawater and the changes in physical and chemical properties; ii) the effects of single mPVC (virgin and aged) and copper on microalgae Chlorella vulgaris ; and iii) the interaction of aged mPVC and copper and the oxidative stress towards C. vulgaris . In this study, some wrinkles, rough and fractured surface textures can be observed on the aged mPVC, accompanying with increased hydroxyl groups and aromatic carbon-carbon double bond but decreased carbon hydrogen bond. It was found that single virgin or aged mPVC at low concentration (10 mg/L) had significant inhibition on the growth of C. vulgaris but no inhibition at higher concentration (100, 1,000 mg/L), which can be reasonably explained by the aggregation and precipitation of mPVC at high concentration. The aging of mPVC inhibited the growth of C. vulgaris with the maximum growth inhibition ratio (IR) of 35.26% as compared with that of virgin mPVC (IR = 28.5%). However, the single copper could significantly inhibit the growth of C. vulgaris and the inhibitory effects increased with concentration (0.2, 0.5, 1.0 mg/L). Furthermore, both the single aged mPVC (10 mg/L) and copper (0.5 mg/L) caused serious cell damage, although the concentration of superoxide dismutase (SOD) and the intracellular malonaldehyde (MDA) increased. In contrast to single treatment, the growth of C. vulgaris can be enhanced by the combined group with copper (0.5 mg/L) and aged mPVC (10 mg/L).
研究领域:
环境科学
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海洋生物学
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生态毒理学
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化学
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DOI
PubMed
高影响因子
Journal of Cleaner Production
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2023
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IF 11.1
Qing Yu,Tian-Ji Liu,Ya-Nan Zeng,Yi-Tong Wang,Jun-Guo Li,Yu-Ran Wang,Le-Le Kang,Rui Ji,Fu-Ping Wang,Xiao-Man Wang,Bao Liu,Shuang Cai,Zhen Fang
Electric furnace slag was highly productive and rich in elements, which can be used as a nutrient source for microalgae growth. The regulation of growth and lipid synthesis of Chlorella pyrenoidosa ( C. pyrenoidosa ) in electric arc furnace slag leaching solution with or without citric acid was investigated to develop efficient lipid production method with low cost. High lipid content of 45.89 wt% from C. pyrenoidosa with adding electric arc furnace slag leaching solution ≤40% into BG11 was obtained due to C. pyrenoidosa increasing lipid accumulation to resist oxidative stress caused by heavy metals in electric arc furnace slag (Ca of 5.13 mg/L, Fe of 0.05 mg/L, Mn of 0.12 mg/L and Cr of 0.01 mg/L). Exogenous citric acid can improve the ability of C. pyrenoidosa to resist heavy metal toxicity with high biomass content (0.44 g/L) achieved. The cooperation of multiple metal ions in electric arc furnace slag shortened the biomass accumulation stage and prolonged and speeded up the lipid synthesis stage of C. pyrenoidosa by modulating PEPC, ACC and ME activities . This study provided guidance for the industrialization of low-cost lipid-rich microalgae and metallurgical solid waste treatment .
研究领域:
环境科学
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生物技术
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微生物学
·
废物管理
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
·
IF 6
Yanan Zhao,Xinyu Zu,Ting Wang,Yan Liang,Yingqiu Li,Chenying Wang,Xiangzhong Zhao,Hua Wang
This article is dedicated to the preparation of a novel Chlorella protein hydrogel that can be used as a slow release carrier. Chlorella proteins and alginate were used as cross-linking agent at the present of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/ N- hydroxysuccinimide to obtain hydrogel with different 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/ N- hydroxysuccinimide concentration. Textural results showed that hydrogel with high N- hydroxysuccinimide concentration possessed good hardness, springiness, gumminess and chewiness, espasically those hydrogel with 23% N- hydroxysuccinimide concentration. Scanning electron microscopy results revealed that the prepared hydrogel with higher N- hydroxysuccinimide concentration had better stable spatial porous structures. Swelling analyses indicated the prepared hydrogel exhibited pH sensitivity, which were beneficial for the slow-release. Then the hydrogels with 23% N- hydroxysuccinimide were used to be poly(lysine) slow-release carriers. Moreover, this hydrogel exhibited a prolonged and efficient period of poly(lysine) slow release at pH 1.2 (30%) and 7.4 (95%) within 200 h, which were further proved by good antibacterial activity against Escherichia coli and Staphylococcus aureus . Finally, based on the above results, we simulated the mechanism of hydrogel formation and poly(lysine) loading and slow release. The results reveal that the prepared Chlorella protein hydrogels are beneficial for use as slow-release carriers.
研究领域:
生物材料学
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食品科学
·
药剂学
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高影响因子
危险品化学品经营许可证（带存储）