Biosensors-Basel
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2022
·Vol. 12
·期 2
·66
·
IF 5.743
Bo Wu,Zehua Li,Zepeng Kang,Chunling Ma,Haiyan Song,Fuping Lu,Zhiguang Zhu
D-2-hydroxyglutaric acid (D2HG) is overproduced as a result of the D-2-hydroxyglutaric aciduria and relevant cancers, caused by gene mutation. Accurate analysis of D2HG could help rapid diagnosis of these diseases and allow for timely treatment. In this work, a D-2-hydroxyglutarate dehydrogenase fromRalstonia solanacearum(RsD2HGDH) is cloned and recombinantly expressed. This enzyme features the direct electron transfer to chemical electron mediators (such as methylene blue (MB)) in the absence of additional coenzymes. Therefore, NAD+, a natural electron acceptor for the commercial D2HGDH and usually known for being unstable and difficult for immobilization can be avoided in the preparation of biosensors. TheRsD2HGDH and MB are co-immobilized on a two-dimensional material, Ti3C2MXene, followed by drop-coating on the gold screen-printed electrode (AuSPE) to construct a compact and portable biosensor. The D2HG in samples can be catalyzed byRsD2HGDH, where the current change is measured by chronoamperometry at −0.23 V. The biosensor shows a D2HG detection range of 0.5 to 120 µM (R2= 0.9974) with a sensitivity of 22.26 μA mM−1cm−2and a detection limit of 0.1 µM (S/N = 3). The biosensor retains 72.52% performance of its incipient state after 30 days of storage. The samples of D2HG-containing fetal bovine serum and artificial urine were analyzed with the recovery of 99.56% to 106.83% and 97.30% to 102.47% further indicating the great application potential of our portable D2HG biosensor.Keywords:D-2-hydroxyglutaric acid;MXene;biosensor;screen-printed electrode;D-2-hydroxyglutarate dehydrogenase
研究领域:
生物化学
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生物技术
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分析化学
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医学诊断
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DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2024
·Vol. 36
·期 39
·2404772
·
IF 27.4
Ning Sun,Zhichuan Zheng,Zhuangzhuang Lai,Junjie Wang,Peng Du,Tianping Ying,Haifeng Wang,Jianchun Xu,Runze Yu,Zhiwei Hu,Chih-Wen Pao,Wei-Hsiang Huang,Ke Bi,Ming Lei,Kai Huang
While high-entropy alloys, high-entropy oxides, and high-entropy hydroxides, are advanced as a novel frontier in electrocatalytic oxygen evolution, their inherent activity deficiency poses a major challenge. To achieve the unlimited goal to tailor the structure–activity relationship in multicomponent systems, entropy-driven composition engineering presents substantial potential, by fabricating high-entropy anion-regulated transition metal compounds as sophisticated oxygen evolution reaction electrocatalysts. Herein, a versatile 2D high-entropy metal phosphorus trisulfide is developed as a promising and adjustable platform. Leveraging the multiple electron couplings and d–p orbital hybridizations induced by the cocktail effect, the exceptional oxygen evolution catalytic activity is disclosed upon van der Waals material (MnFeCoNiZn)PS 3 , exhibiting an impressively low overpotential of 240 mV at a current density of 10 mA cm −2 , a minimal Tafel slope of 32 mV dec −1 , and negligible degradation under varying current densities for over 96 h. Density functional theory calculations further offer insights into the correlation between orbital hybridization and catalytic performance within high-entropy systems, underscoring the contribution of active phosphorus centers on the substrate to performance enhancements. Moreover, by achieving electron redistribution to optimize the electron coordination environment, this work presents an effective strategy for advanced catalysts in energy-related applications.
研究领域:
材料科学
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电化学
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能源转换与存储
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DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2023
·
IF 5.9
Yidong Liu,Weipeng Chen,Heng Liu,Jiangrong Luo,Li Zhao,Jisheng Zhang,Huayu Wang,Jing-Wu Wu,Jun-Li Feng,Yongxiang Zhu,Wan-Yi Tan,Tingting Cui,Yonggang Min
Polyimide (PI) possesses multiple advantages including excellent thermal stability, mechanical property and chemical resistance, which has been considered as one of the promising candidates for high-performance electrical insulation materials. Optimizing the polymer structure such as extending the band gap can yield high intrinsic breakdown strength, attributed to the inhibition of impact ionization. However, in most cases, large band gap is achieved at the cost of reducing the backbone coplanarity and rigidity or the intermolecular interaction, resulting in undesirable thermal and mechanical performance. Herein, we introduce an effective strategy to produce thermally stable and mechanically strong polyimide insulation with high breakdown strength through introducing aromatic donor groups into the main chains to construct a D−π−A structure. On the one hand, the formation of D−A effect increases the materials polarization and enhances dipole-induced scattering. On the other hand, enhanced intermolecular interaction via π−π stacking decreases free volume in PIs. Then, increased conjugation degree of the backbone and high packing density between the polymer chains benefit to thermal stability and mechanical properties. Based on this strategy, N -arkyl carbazole groups are preferred, in which the soft alkyl side groups can occupy the pores between the polymer chains to further decrease free volume. As a result, we afford polyimide insulation with significantly improved breakdown strength from 291 kV mm −1 to 570−632 kV mm −1 , as compared to the reference PI ODA-PMDA. Simultaneously, the resultant PIs, such as PI-Bu, preserve high T d5% above 524 ℃, high T g above 364 ℃ and tensile strength above 80 MPa.
研究领域:
材料科学
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电气工程
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2025
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IF 3.3
He Ying,Huang Ao,Liu Yun
Phospholipase D (PLD) is a valuable enzyme in industrial processes for converting phosphatidylcholine (PC) to phosphatidylserine (PS). In this study, a strain of Bacillus cereus was isolated from soil and identified through 16S rRNA sequencing. To enhance PLD activity, various mutagenesis strategies—including chemical treatment, irradiation, and their combinations—were employed, resulting in four high-activity positive mutants (C-7, I-12, CI 7–12, and IC 13–14). Among these, the CI 7–12 mutant exhibited a significantly higher enzymatic activity, showing a 3.12-fold increase (312.2%) compared to the wild-type strain. Fermentation conditions were optimied using response surface methodology (RSM), achieving a PLD activity of 35 U/mL. The enzyme demonstrated stability over a wide temperature range (30–60 °C) and pH range (6–10), with a half-life of 128 days. Kinetic analysis revealed a V max of 20.04 μmol/h and a K m of 7.13 μmol/mL, indicating efficient activity. In bioconversion experiments, the PLD-enriched fermentation broth catalyzed the conversion of PC to PS, achieving a 53.0% conversion rate and a 92.3% selectivity for PS in a two-phase system. These findings expand the potential sources of PLD and underscore its applicability for PS production in biotechnological applications.
研究领域:
微生物学
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生物化学
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工业生物技术
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2018
·
IF 4.513
Kemeng Shi,Christian Marcus Pedersen,Zhaohui Guo,Yanqiu Li,Hongyan Zheng,Yan Qiao,Tuoping Hu,Yingxiong Wang
The tautomer distributions of d ‑fructose, a popular model compound for biorefinery , in various lower alcohols and DMSO‑ d 6 (ROHs/DMSO‑ d 6 ) mixtures at 298 K were investigated by quantitative 13 C NMR. The tautomer distributions of d ‑fructose in ethanol/DMSO‑ d 6 and methanol/DMSO‑ d 6 with different volume ratios of alcohols to DMSO‑ d 6 were also examined. For these normal alcohols (methanol, ethanol, n ‑propanol and n ‑butanol)/DMSO‑ d 6 , the tautomer distribution of d ‑fructose in methanol/DMSO‑ d 6 exhibited the shortest equilibration time (46 h) and the lowest concentration of β‑furanose (39.0%) at equilibrium. The fraction of fructofuranose (α‑furanose and β‑furanose) at equilibrium and time needed to reach equilibrium in branched chain alcohols/DMSO‑ d 6 obey the following order: 2‑propanol/DMSO‑ d 6 (62.2%, 320 h) > tert ‑butanol/DMSO‑ d 6 (61.6%, 307 h) > 2‑butanol/DMSO‑ d 6 (60.6%, 216 h) > isobutanol/DMSO‑ d 6 (57.8%, 134 h). For the diols/DMSO‑ d 6 , the time for the tautomer distribution of d ‑fructose to reach the equilibrium was found to be: 1,4‑butanediol/DMSO‑ d 6 (113 h) > 1,2‑propanediol/DMSO‑ d 6 (38 h) > ethylene glycol/DMSO‑ d 6 (36 h). For ethanol/DMSO‑ d 6 and methanol/DMSO‑ d 6 with respectively different volume ratios, the experimental results showed that the high concentration of ethanol or methanol increases the conversion rate of tautomers.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2023
·
IF 4.8
Ying-ying Hu,Xiao-qin Wu,He-ping Shi,Xue-hong Wei
The nature of amino acids as the building blocks of proteins leading to detection and identification is a current hot topic. Therefore, {[Zn 3 (L − ) 2 (HBTC 2− ) 2 ]·2H 2 O·CH 3 CN} n ( Zn-1 , H 3 BTC = 1,3,5-benzenetricarboxylic acid, HL = 6-aminonicotinic acid, CH 3 CN = acetonitrile), a 2D Zn-MOF which can sensitively detect and selectively recognize D -tyrosine was synthesized by solvothermal method . Identification of D -tyrosine was determined by increasing fluorescence intensity and red-shifting of the emission wavelength . Zn-1 is highly stable in methanol (MeOH) and aqueous solution (H 2 O), so it was dissolved in them as fluorescence probes, namely Zn-1/MeOH probe and Zn-1/H 2 O probe , respectively. The two probes worked for lower limits of detection, 8.93 × 10 −2 μM for Zn-1/MeOH probe and 79.70 × 10 −2 μM for Zn-1/H 2 O probe . The Stern-Volmer quenching constant of Zn-1/MeOH probe is 1.28 × 10 4 M −1 and of Zn-1/H 2 O probe is 4.55 × 10 2 M −1 . In addition, the two probes still show excellent immunity to interference under the interference of 18 amino acids. Zn-1/H 2 O probe was also applied to the determination of D -tyrosine in real milk samples with good results. This study provides a fast, simple, convenient and relatively accurate method for detecting D -tyrosine.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Yulin Fan,Fang Fu,Langqiu Chen,Jiping Li,Jing Zhang
Dioxyethene fragment (−(OCH2CH2)2−) was introduced into traditional alkyl β-d-glucopyranosides to ameliorate the water solubility, and eight nonionic surfactants, that is, alkoxy ethoxyethyl β-d-glucopyranosides with alkyl chain lengths (n = 6–16), were synthesized and characterized. Their hydrophilic and lipophilic balance number, water solubility, critical micelle concentration (cmc), γcmc, Γmax, and hygroscopic rate decreased with an increase in the alkyl chain length. Hexadecoxy ethoxyethyl β-d-glucopyranoside had no water solubility at 25 °C. Decoxy ethoxyethyl β-d-glucopyranoside had the best emulsifying property in the toluene/water and n-octane/water systems and the strongest foaming property, whereas dodecoxy ethoxyethyl β-d-glucopyranoside had the best emulsifying property in the rapeseed oil/water system. Such β-d-glucopyranosides (n = 6–12) exhibited excellent surface activity. In addition, for the binary mixture of alkoxy ethoxyethyl β-d-glucopyranosides (n = 8, 10, 12) and sodium dodecyl sulfate or cetyl trimethyl ammonium chloride, their cmc values were lower than the pure β-d-glucopyranosides, indicating that they had synergistic interactions. The fan focal conic textures of alkoxy ethoxyethyl β-d-glucopyranosides (n = 7–16) were observed during the cooling process under a polarizing optical microscope. Alkoxy ethoxyethyl β-d-glucopyranosides (n = 14, 16) had the related melting points and the clear points with differential scanning calorimetry. With β-d-glucopyranosides (n = 6–16) and n-butanol as the surfactant and cosurfactant, respectively, and with cyclohexane as the oil phase, the related microemulsion areas in their pseudoternary phase diagram system were investigated with the visual observation at 25 °C. Along with the slashing requirements of petroleum consumption, environmental protection, and green and sustainable development, nonionic sugar-based alkoxy ethoxyethyl β-d-glucopyranosides should be expected to have their potential practical application because of their strengthened hydrophilicity, improved water solubility, and enhanced surface activity.
研究领域:
化学
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材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2014
·
IF 2.819
Mohamed Reda Zahi,Hao Liang,Qipeng Yuan
The purpose of this research was to develop a novel antimicrobial delivery system by encapsulating d -limonene into an organogel-based nanoemulsion and investigating its antimicrobial activity. The d -limonene organogel-based nanoemulsion was prepared by high pressure homogenization method. The surfactant concentration had a major impact on the droplets' formation and distribution. At the optimal condition (10% w/w Tween 80, 100 Mpa, and 10 Cycles) the smallest droplet size ( d ≈ 36 nm) could be obtained, which has shown a narrow structure and good stability. Results from the antimicrobial activity have shown the encapsulation of d -limonene (4% w/w) into the organogel-based nanoemulsion contributed to the increase of its antimicrobial activity. In addition, the mechanism of d -limonene organogel-based nanoemulsion against the tested microorganisms was studied by the electronic microscope observation and the cell constituent release. This research would have an important implication for the design of more efficient antimicrobial systems for food preservation and production.
研究领域:
食品科学
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微生物学
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材料科学
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DOI
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2024
·
IF 3.1
Yuan Li,Chun You,Yi-Heng P. Job Zhang
d-Glucaric acid (GA), one of the polyhydroxy dicarboxylic acids, is known for its great potentials in the polymer, food, detergent, and pharmaceutical industries. Here, we presented a highly efficient synthesis of GA from starch by in vitro biotransformation (ivBT). This one-pot ivBT was composed of five thermophilic enzymes (i.e., isoamylase, α-glucan phosphorylase, phosphoglucomutase, inositol 1-phosphate synthase, and inositol monophosphatase) responsible for the synthesis of myo-inositol (MI) from starch, followed by three mesophilic enzymes (i.e., myo-inositol oxygenase (MIOX), uronate dehydrogenase (UDH), and H2O-forming NADH oxidase (NOX)) responsible for the synthesis of GA from inositol. Also, a stepwise-added enzyme cocktail strategy was employed to circumvent the inhibition of GA on MIOX and mismatches in enzymes’ properties, such as temperature and pH. The three-step cascade bioprocessing in one pot resulted in the concentration of disodium glucarate of approximately 9.66 g/L (i.e., 38 mM) from 10 g/L maltodextrin with an atom efficiency of 96.6%. Up to 52.1 g/L disodium d-glucarate, the highest titer reported, was produced in a stirred bioreactor operated in two-reaction temperature ranges, featured with the stepwise addition of enzyme cocktails, and equipped with a constant pH controller and stirring for enough oxygen supplies. This study could open a new door to the industrial biomanufacturing of GA by ivBT.
研究领域:
生物化学
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工业生物技术
✓ 已验证
DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2013
·
IF 3.607
Jingjing Li,Kaiqi Fan,Libo Niu,Yuanchao Li,Jian Song
The effect of salt on the gelatinization of 2,4-(3,4-dichlorobenzylidene)-d-sorbitol (DCBS), a novel low-molecular-weight gelator, was studied. DCBS showed pronounced hydrogelation and the electron micrographs indicated that the hydrogels consists of globular aggregates. Addition of NaCl to the aqueous medium accelerated the gelation process and also caused the gel’s morphology to change from globular to long fibers. In addition, the thermal properties of the hydrogels were improved with the addition of NaCl. UV–vis and fluorescence emission spectra showed that extensive aggregation of the phenyl rings was responsible for the gelation. The presence of NaCl induced a red shift in the emission peaks of DCBS and a decrease of the pyrene polarity index I1/I3 in the gels, which indicated that there was more π–π stacking in the hydrogels with NaCl than in the gels without NaCl. Variable-temperature 1H NMR spectra further demonstrated that the π–π interactions were enhanced by NaCl. FTIR studies showed that hydrogen bonding was also a contributing factor in the gelation process. Wide-angle X-ray diffraction (WXRD) showed that the hydrogels had a layered structure which did not change with the addition of NaCl. Density functional theory (DFT) calculations indicated the possible molecular packing of the gelator in the nanofibers.
研究领域:
材料科学
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化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemSusChem
·
2021
·Vol. 14
·期 23
·5311-5319
·
IF 8.928
Jianwang Xu,Xinge Miao,Lei Liu,Yantao Wang,Weiran Yang
Graphical Transfer hydrogenation : 5-Methylfurfural (5-MF), a promising C 6 platform molecule, is directly produced from biomass-derived carbohydrates by iodide-mediated transfer hydrogenation. Under the optimized conditions, 30–50 % of 5-MF is yielded from low-cost sugars and starch with 5-MF productivity as high as 103 mmol g cat −1 h −1 . The catalytic system is simple, robust, recyclable, and preferable to other approaches for the production of 5-MF. Herein, a robust catalytic system was developed for the green synthesis of 5-methylfurfural (5-MF) by iodide-mediated transfer hydrogenation. Around 50 % of 5-MF was yielded from d -fructose within 7.5 min using NaI as the catalyst and formic acid as both the hydrogen source and co-catalyst. The catalytic system was used for six consecutive cycles without any decrease in the yield. Various starch and raw biomass could be used as promising starting materials for 5-MF synthesis with moderate yields, and the productivity of 5-MF from corn starch reached 103 mmol g cat −1 h −1 , which is comparable with the best result from l -rhamnose. Moreover, the co-production of 5-MF and furfural from raw biomass makes this methodology more competitive than other routes.
研究领域:
化学工程
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可再生资源
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催化化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yan Liu,Ling Zhang,Kaiyang Guo,Jingyuan Zhao,Yonglei Xing,Zhuosen Wang,Yapeng Tian,Xinwei Cui
Intercalation pseudocapacitance is the key point to achieving the balance between the volumetric capacitance and the rate performance of energy storage. However, the low pseudocapacitive reactivity of the active center still limits the energy density of the pseudocapacitance devices. Herein, we propose a strategy that regulates the p - d hybridization of confined MXene via the co-doping of B and O atoms (BO-MXene) for outstanding electrochemical performance. Both experiments and density functional theory (DFT) reveal that the inner doped O atoms in low 2 p orbital energy strengthen the hybridization of Ti and electron-deficient B in MXene for high retention of B atoms, weaken the Ti-O covalency in MXene. As a result, the weakened p - d hybridization of Ti and O for the outer surface and strengthened p - d hybridization of Ti and B for the inner, promote the adsorption of protons and electron transfer, respectively. Further, the BO-MXene-based hybrid supercapacitors achieve a significant volumetric energy density of 56.86 Wh L -1 at 25.6 kW L -1 . This electronic structure via tuning the p - d hybridization in MXene proposes a new path for designing proton-confined intercalation pseudocapacitance.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）