ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Qingying Shi,Jingchun Tang,Lan Wang,Rutao Liu,John P. Giesy
Awareness of risks posed by widespread presence of nanoplastics (NPs) and bioavailability and potential to interact with organic pollutants has been increasing. Inhalation is one of the more important pathways of exposure of humans to NPs. In this study, combined toxicity of concentrations of polystyrene NPs and various phthalate esters (PAEs), some of the most common plasticizers, including dibutyl phthalate (DBP) and di-(2-ethyl hexyl) phthalate (DEHP) on human lung epithelial A549 cells were investigated. When co-exposed, 20 μg NPs/mL increased viabilities of cells exposed to either DBP or DEHP and the modulation of toxic potency of DEHP was greater than that of DBP, while the 200 μg NPs/mL resulted in lesser viability of cells. PAEs sorbed to NPs decreased free phase concentrations ( C free ) of PAEs, which resulted in a corresponding lesser bioavailability and joint toxicity at the lesser concentration of NPs. The opposite effect was observed at the greater concentration of NPs, which may result from the dominated role of NPs in the combined toxicity. Furthermore, our data showed that oxidative stress and inflammatory reactions were mechanisms for combined cytotoxicities of PAEs and NPs on A549 cells. Results of this study emphasized the combined toxic effects and mechanisms on human lung cells, which are helpful for assessing the risk of the co-exposure of NPs and organic contaminants in humans.
研究领域:
环境科学
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毒理学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Min Sun,Juanjuan Feng,Xiangping Ji,Chunying Li,Sen Han,Mingxia Sun,Yang Feng,Jiaqing Feng,Haili Sun
To improve extraction performance of carbon fibers (CFs) towards phthalate esters (PAEs), titanium dioxide (TiO 2 ) nanorods array was in-situ grown on the surface of CFs, then polyaniline (PANI) was used to modify it. PANI/TiO 2 nanorods-CFs were placed into a polyetheretherketone tube for solid-phase microextraction (SPME). Combining the tube to high performance liquid chromatography (HPLC), it was evaluated and displayed good extraction performance for several PAEs. Compared with bare CFs, TiO 2 nanorods and PANI, PANI/TiO 2 nanorods presented best performance, attributed to the unique advantages between high surface area of TiO 2 nanorods and multiple adsorption interactions (like π-π stacking, hydrogen bond ) of PANI. After the optimization of the important factors (sampling volume, sampling rate, sample pH, concentrations of organic solvent and salt in sample, and desorption time), the online in-tube SPME-HPLC method was established. It provided low limits of detection (0.01-0.05 μg L −1 ) and wide linear ranges (0.03-30, 0.10-30, 0.17-30 μg L −1 ) with correlation coefficients larger than 0.9991. The relative standard deviations (n=6) between intra-day and inter-day tests were in the ranges of 3.5-10.3% and 4.7-13.9%, respectively. The method was successfully used to determine seven PAEs in real water samples. Besides of satisfactory durability, the material also exhibited superior extraction performance than some materials.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 31
·31091-31100
·
IF 2.8
Lei Yu,Zhu Chengzhu,Lu Jun,Zhu Yongchao,Zhang Qiuyue,Chen Tianhu,Xiong Hongbin
The photochemical oxidation of di- n -butyl phthalate (DBP) by • OH radicals from nitrous acid (HONO) in atmospheric hydrometeors was explored by two techniques, steady-state irradiation, and laser flash photolysis (LFP). The effects of atmospheric liquid parameters on DBP transformation were systematically evaluated, showing that DBP does not react with HONO directly and • OH-initiated reactions are crucial steps for consumption and transformation of DBP. Two reaction channels are operative: • OH addition and hydrogen atom abstraction. The overall rate constant for the reaction of DBP with • OH is 5.7 × 10 9 M −1 s −1 , and its specific rate constant for addition is 3.7 × 10 9 M −1 s −1 determined by using laser flash photolysis technique. Comparing the individual reaction rate constant for aromatic ring addition with the total rate constant, the majority of the • OH radicals (about 65%) attack the aromatic ring. The major transformation products were identified by GC-MS, and the trends of their yields derived from both ring addition and H-abstraction with time are discussed. These results provide important insights into the photochemical transformation of DBP in atmospheric hydrometeors and contribute to atmospheric aerosol chemistry.
研究领域:
环境科学
·
大气化学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2016
·
IF 3.976
Hai-Ming Zhao,Huan Du,Jing Lin,Xue-Bin Chen,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Hua-Ming Qin,Ming-Hung Wong
A newly isolated strain Agromyces sp. MT-O could utilize various phthalates and efficiently degraded di-(2-ethylhexyl) phthalate (DEHP). Response surface methodology was successfully employed for the optimization of culture conditions including pH (7.2), temperature (29.6), and inoculum size (OD 600 of 0.2), resulting in almost complete degradation of DEHP (200 mg L − 1 ) within 7 days. At different initial concentrations (50–1000 mg L − 1 ), DEHP degradation curves were fitted well with the first-order kinetic model, and the half-life of DEHP degradation ranged from 0.83 to 2.92 days. Meanwhile, the substrate inhibition model was used to describe the special degradation rate with q max , K s , and K i of 0.6298 day − 1 , 86.78 mg L − 1 , and 714.3 mg L − 1 , respectively. The GC–MS analysis indicated that DEHP was degraded into mono-ethylhexyl phthalate and phthalate acid before its complete mineralization. Bioaugmentation of DEHP-contaminated soils with strain MT-O has greatly enhanced DEHP disappearance rate in soils, providing great potential for efficiently remediating DEHP-contaminated environment.
研究领域:
环境科学
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微生物学
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生物技术
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DOI
PubMed
高影响因子
TALANTA
·
2013
·
IF 3.498
Xi Luo,FeiFang Zhang,Shunli Ji,Bingcheng Yang,Xinmiao Liang
Graphene nanoplatelet (GN) as a solid-phase extraction (SPE) sorbent in combination with high performance liquid chromatography has been used for the determination of five phthalate esters (PAEs) in aqueous solution. The operation parameters affecting the extraction efficiency were optimized. Comparative studies showed that GN was superior to other common SPE sorbents in terms of recovery and adsorption capacity. Under optimization conditions, detection limits of 0.09–0.33 ng mL −1 were achieved for five PAEs and enrichment factors of 402–711 for the analytes were obtained. The proposed method was successfully applied for the determination of PAEs in tap water and drink samples with recoveries ranging from 87.7% to 100.9%.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Yangyang Wang,Wenhao Zhan,Qiang Ren,Shanshan Cheng,Junnan Wang,Xiaoyu Ma,Chaosheng Zhang,Yansong Wang
A bacterial strain ( Gordonia sp. Lff) capable of efficiently degrading di-(2-ethylhexyl) phthalate (DEHP) was isolated from river sludge. The optimal pH and temperature for the degradation of DEHP by Lff were 7.0 and 35 °C, respectively. Lff could degrade high concentrations of DEHP (100–2000 mg/L) with a degradation efficiency of over 91.43%. The DEHP degradation curves fit well with first-order kinetics, with a half-life ranging from 0.598 to 0.746 d. Substrate inhibition analyses showed that the maximum specific degradation rate, half-saturation constant and inhibition constant were 0.8 d −1 , 45.8 mg/L and 462.18 mg/L, respectively. A detailed biodegradation pathway of DEHP was proposed based on GC–MS analysis. Furthermore, Lff could also efficiently degrade DEHP in soils. DEHP or DEHP plus Lff changed the bacterial community in soils, and Lff accelerated the shaping of the bacterial community. To the best of our knowledge, this study is the first to perform a detailed investigation into the biodegradation of DEHP in soil by Gordonia sp. and its effect on the soil bacterial community. These results suggest that Lff is an ideal candidate for the bioremediation of DEHP-contaminated environments.
研究领域:
环境微生物学
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土壤学
·
生物修复
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DOI
PubMed
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2022
·
IF 4.907
Xiaoyong Song,Zihan Zhang,Yanran Dai,Deshou Cun,Baihui Cui,Yuewei Wang,Yaocheng Fan,Haibin Tang,Liang Qiu,Feihua Wang,Dongru Qiu,Wei Liang
Di-(2-ethylhexyl) phthalate (DEHP), the most extensively used phthalate acid esters (PAEs), poses a potential risk to human and environment. A novel bacterial strain , Rhodococcus sp. LW-XY12, with efficient PAE-degrading capability, was isolated from activated sludge. Strain LW-XY12 could degrade 96.91 ± 0.68% of DEHP (500 mg L −1 ) within 32 h. The degradation curves of DEHP (50–1500 mg L −1 ) and PAE mixture (500 mg L −1 each) fitted well with the modified Gomperz kinetics model. DEHP metabolic pathway was reconstructed by using genome annotation and metabolic intermediate analyses. The DEHP metabolic pathway might comprise de-esterification and β-oxidation. Homologous modeling and molecular docking analysis revealed that DEHP and MEHP (mono-(2-ethylhexyl) phthalate) could be bound to putative carboxylesterase (KXC42_04905) via hydrogen bonding and hydrophobic interaction. A conserved catalytic triad (Ser195-Glu319-His412) might act as an active protein pocket and catalyze the hydrolysis of DEHP. Carboxylesterase (KXC42_04905) could hydrolyze ester bond and a possible mechanism underlying ester bond hydrolysis catalyzed by the carboxylesterase was proposed. Protocatechuate 3,4-dioxygenase, 3-oxoadipate CoA-transferase, benzoate 1,2-dioxygenase, catechol 1,2-dioxygenase, and catechol 2,3-dioxygenase transcripts were detected and significantly up-regulated under DEHP induction. This study sheds light on the functional genes associated with DEHP degradation and metabolic mechanism in this versatile bacterium for degradation of PAEs.
研究领域:
环境微生物学
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基因组学
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生物降解
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生物化学
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产品关联
DOI
高影响因子
Toxics
·
2023
·Vol. 11
·期 5
·469
·
IF 4.6
Qiang Zhang,Wenjie Ma,Jingmin Zhu
The increasing concern for the ecological risks of microplastics (MPs) as carriers of hydrophobic organic contaminants is evident. Di-butyl phthalate (DBP) is extensively utilized as an additive in plastic products, and both DBP and MPs are widespread in the environment. However, the combined toxicity of these substances remains uncertain. In this study, zebrafish embryos were employed to assess the toxic effects of polyethylene terephthalate (PET, MPs) and DBP, with a focus on the DBP toxicities influenced by PET. The embryonic chorion was partially covered by PET particles, and PET led to a delayed hatching of zebrafish embryos without inducing death or teratogenesis. On the other hand, exposure to DBP considerably inhibited the hatching of embryos, leading to severe lethal and teratogenic effects. The most common phenotypes induced by DBP exposure were delayed yolk sac absorption and pericardial edema. The mortality increased in co-treatment with 100 particles/mL PET and 2 mg/L DBP at 24 hpf and 48 hpf. The malformation phenotype, bent notochord, and delayed yolk sac absorption became more severe in 1 mg/L DBP exposition with the co-exposure of 100 particles/mL PET at 72 hpf. PET might act as a carrier that enhances the bioavailability of ambient DBP.Keywords:polyethylene terephthalate;di-butyl phthalate;zebrafish embryos;malformation phenotype;transport carrier
研究领域:
环境科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2017
·Vol. 12
·期 5
·e0178213
·
IF 2.806
Yangyang Wang,Fangfang Li,Xinling Ruan,Jian Song,Lv Lv,Liyuan Chai,Zhihui Yang,Lin Luo
A stable bacterial consortium (LV-1) capable of degrading di- n -butyl phthalate (DBP) was enriched from river sludge. Community analysis revealed that the main families of LV-1 are Brucellaceae (62.78%) and Sinobacteraceae (14.83%), and the main genera of LV-1 are Brucella spp. (62.78%) and Sinobacter spp. (14.83%). The optimal pH and temperature for LV-1 to degrade DBP were pH 6.0 and 30°C, respectively. Inoculum size influenced the degradation ratio when the incubation time was < 24 h. The initial concentration of DBP also influenced the degradation rates of DBP by LV-1, and the degradation rates ranged from 69.0–775.0 mg/l/d in the first 24 h. Degradation of DBP was best fitted by first-order kinetics when the initial concentration was < 300 mg/l. In addition, Cd 2+ , Cr 6+ , and Zn 2+ inhibited DBP degradation by LV-1 at all considered concentrations, but low concentrations of Pb 2+ , Cu 2+ , and Mn 2+ enhanced DBP degradation. The main intermediates (mono-ethyl phthalate [MEP], mono-butyl phthalate [MBP], and phthalic acid [PA]) were identified in the DBP degradation process, thus a new biochemical pathway of DBP degradation is proposed. Furthermore, LV-1 also degraded other phthalates with shorter ester chains (DMP, DEP, and PA).
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2017
·
IF 2.603
Zigang Tang,Lanling Chu,Yu Wang,Yuan Song,Ping Liu,Jinhui Fan,Jinjin Huang,Xiongwei Liu,Lanlan Wei,Chen Li,Renshan Zhao,Xuejun Kang
In this paper, we developed a rapid and safe method based on packed-fiber solid-phase extraction (PFSPE) system coupled with gas chromatography-mass spectrometry for the determination of four phthalate esters (PAEs), diethyl-o-phthalate (DEP), dibutyl-o-phthalate (DBP), di (2-ethylhexyl) phathalate (DEHP), di- n -octyl phthalate (DNOP), in urine samples. The PAEs in urine samples (500 μL) were rapidly cleaned up from urines using polystyrene (PS) nanofibers packed micro-columns fitted on a PFSPE pretreatment device, which can process up to 12 samples simultaneously in 5 min. Under optimum conditions, satisfied recovery and relative standard deviation values (RSDs) were in the range of 80.4–111.7% and 1.5–10.9%, respectively. The limits of detection (LOD) and the limits of quantification (LOQ) were ranged from 0.1 to 0.5 ng mL −1 and 0.5–2 ng mL −1 , respectively. The well controlled matrix effect was also evaluated by comparing the signal response of the pure PAEs standards dissolved in methanol with the signal response of PAEs in the urine matrix. This new method was successfully applied to determine four PAEs in urine samples of overweight and normal-weight children, and an association between phthalates in urines and obesity was observed. Thus, the method seems to be a useful tool for monitoring of the level of urinary phthalate esters and also to support an evidence for further reasearch of obesity.
研究领域:
环境科学
·
分析化学
·
公共卫生
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产品关联
DOI
PubMed
高影响因子
PEDOSPHERE
·
2017
·
IF 1.734
Jiajun DENG,Yanling ZHANG,Jiwei HU,Jiaguo JIAO,Feng HU,Huixin LI,Shixiang ZHANG
Autotoxicity is one of the major factors that impede continuous cropping. It is defined as the toxic influence of chemicals released from one plant species on the germination and growth of individuals of the same species. Here, in order to exam the autotoxicity of tobacco root exudates, root exudates were collected from tobacco plants grown both in cultural solution and on natural soil. Using ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, main autotoxic chemical substances in the root exudates were identified. The autotoxic effects of suspected autotoxins on seed germination (including germination rate, germination potential, germination index, and vigor index) and seedling growth were analyzed. Dibutyl phthalate (or diisobutyl phthalate), dioctyl phthalate, and diisooctyl phthalate were identified in tobacco root exudates. It was observed that high concentrations (greater than 0.5 mmol L −1 ) of each identified phthalate ester caused significant ( P < 0.05) inhibition of tobacco seed germination and seedling growth. It can be concluded that phthalate esters such as dibutyl phthalate, diisobutyl phthalate, and diisooctyl phthalate in tobacco root exudates may play an important role in tobacco autotoxicity.
研究领域:
植物学
·
生态学
·
农业科学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2014
·
IF 4.331
Zhixing He,Chengzhen Niu,Zhenmei Lu
The bacterial strain DP-2, identified as Rhodococcus ruber , is able to effectively degrade di-n-butyl phthalate (DBP) and phenol. Degradation kinetics of DBP and phenol at different initial concentrations revealed DBP and phenol degradation to fit modified first-order models. The half-life of DBP degradation ranged from 15.81 to 27.75 h and phenol degradation from 14.52 to 45.52 h under the initial concentrations of 600–1200 mg/L. When strain DP-2 was cultured with a mixture of DBP (800 mg/L) and phenol (700 mg/L), DBP degradation rate was found to be only slightly influenced; however, phthalic acid (PA) accumulated, and phenol degradation was clearly inhibited during synchronous degradation. Transcriptional levels of degradation genes, phenol hydroxylase ( pheu ) and phthalate 3,4-dioxygenase ( pht ), decreased significantly more during synchronous degradation than during individual degradation. Quantitative estimation of individual or synchronous degradation kinetics is essential to manage mixed hazardous compounds through biodegradation in industrial waste disposal.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）