Foods
·
2024
·Vol. 13
·期 8
·1158
·
IF 4.7
Yuzhu Zhao,Doudou Liu,Jiaxuan Zhang,Jiaxin Shen,Jiamin Cao,Huawei Gu,Mengqing Cui,Ling He,Gong Chen,Shuwen Liu,Kan Shi
Grape pomace seeds contain abundant phenolic compounds, which are also present in both soluble and insoluble forms, similar to many other plant matrices. To further increase the extractable soluble phenolics and their antioxidant activities, grape pomace seeds were fermented with different fungi. Results showed that solid-state fermentation (SSF) withAspergillus niger,Monascus anka, andEurotium cristatumat 28 °C and 65% humidity had a significantly positive impact on the release of soluble phenolics in grape pomace seeds. Specifically, SSF withM. ankaincreased the soluble phenolic contents by 6.42 times (calculated as total phenolic content) and 6.68 times (calculated as total flavonoid content), leading to an overall improvement of antioxidant activities, including DPPH (increased by 2.14 times) and ABTS (increased by 3.64 times) radical scavenging activity. Furthermore, substantial changes were observed in the composition and content of individual phenolic compounds in the soluble fraction, with significantly heightened levels of specific phenolics such as chlorogenic acid, syringic acid, ferulic acid, epicatechin gallate, and resveratrol. Notably, duringM. ankaSSF, positive correlations were identified between the soluble phenolic content and hydrolase activities. In particular, there is a strong positive correlation between glycosidase and soluble phenols (r = 0.900). The findings present an effective strategy for improving the soluble phenolic profiles and bioactivities of grape pomace seeds through fungal SSF, thereby facilitating the valorization of winemaking by-products.
研究领域:
食品科学
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生物技术
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化学
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农业
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2015
·
IF 2.837
Shanshan Wang,Dongmei Wang,Zehua Liu
Potentilla fruticosa and EGb 761 ( Ginkgo biloba extracts) have been applied in traditional medicine and exhibit antioxidant properties. However, there is very little scientific evidence to support their use in combination. In this study, a bio-guided chromatographic fractionation strategy was used to obtain two effective fractions (C-3 and D-2) from the acetone crude extracts (A) of P. fruticosa leaves. Isobolographic analysis indicated that the combination of A + EGb had largely demonstrated synergism and 5:1 (A:EGb) displayed the best synergistic effect. In addition, C-3 + EGb exhibited a similar result as A + EGb, synergism in DPPH and ABTS assays, additive in FRAP assay. While D-2 + EGb showed only additive effect. The possible mechanism behind the synergism was explored by RP-HPLC. The changes in the total phenolic and flavonoids content were also investigated. While chemical composition did not seem to change in the mixture, no new peaks appeared in the chromatogram of C-3 + EGb mixture as compared to the individual chromatograms, no existing peaks disappeared either. Further studies were observed which may prove the possible mechanism responsible for the synergistic effect. Combinations of C-3 and 4 phenolic compounds in EGb ((+)-catechin, kaempferide, quercetin and isorhamnetin) were evaluated for their antioxidant capacities. Isorhamnetin showed synergism with C-3, and 5:1 exhibited the strongest synergism in DPPH ( γ = 0.669) and ABTS ( γ = 1.249), 1:1 in FRAP assays ( γ = 1.385). Isorhamnetin was added to the other 6 phenolics in C-3 ((+)-catechin, caffeic acid, hyperoside, rutin, ellagic acid and quercetin), and the antioxidant capacity of mixtures was analyzed. For all combinations studied, caffeic acid + isorhamnetin displayed the greatest synergistic effect in various ratios by three antioxidant assays, the observed values were significantly higher than the expected values ( P < 0.05) and 1:1 exhibited the strongest synergism in DPPH ( γ = 0.711), ABTS ( γ = 1.693) and FRAP ( γ = 1.474) assays. Hyperoside + isorhamnetin also showed a slight synergism, but (+)-catechin + isorhamnetin exhibited an antagonism in DPPH assays. Thus, interaction of phytochemicals exist in extracts is likely responsible for the synergistic effects present in A + EGb, and caffeic acid + isorhamnetin may have played an important role. This study lends some credence to the concomitant use of P. fruticosa blended with EGb, which could be useful for developing new medicines and functional products from P. fruticosa and EGb.
研究领域:
药理学
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生物化学
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药物化学
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产品关联
DOI
高影响因子
Journal of Aquatic Food Product Technology
·
2017
·
IF 0.478
Jia-Run Han,Zhi-Mo Zhu,Hai-Tao Wu,Na Sun,Yue Tang,Cui-Ping Yu,Chen-Chen Zhao,Zheng-Yu Zhang,Ao-Ting Li,Jia-Nan Yan
Maillard reaction products (MRPs) were produced in sea cucumber gut hydrolysates (SCGHs)-ribose system at 75°C, 85°C, and 95°C with a pH value of 7.0 or 8.0 for 12 hour. The parameters of amount of intermediate products, fluorescence intensity, browning development, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability showed a positive correlation with each other in SCGHs-ribose system. The amount of intermediate products, fluorescence intensity, and browning development followed the zero-order kinetic with activation energy from 62 to 91 kJ/mol in the Maillard reaction of SCGHs-ribose system. Moreover, 2-methyl-butanal has positive and significant correlation with DPPH radical scavenging ability of SCGHs-ribose MRPs. These results suggest that Maillard reaction improves the antioxidant activity of SCGHs, which contributes to the generation of intermediate, fluorescent, browning, and volatile products of SCGHs-ribose system.
研究领域:
食品化学
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营养科学
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生物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Yidan Zhang,Xu Jing,Zhenjia Chen,Xiaowen Wang
In this paper, the preparation, purification, and identification of the antioxidant peptides of millet gliadin after high hydrostatic pressure (HHP) treatment were studied to broaden the utilization range of millet and provide certain theoretical basis for its further processing and utilization. Millet gliadin treated with 300 MPa was enzymatically hydrolyzed by pepsin, and the optimal hydrolysis time was determined to be 50 min by in-vitro antioxidant activity evaluation method [1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical, hydroxyl radical, and superoxide anion scavenging activity]. The enzymatic hydrolysate was separated and purified by gel filtration and RP-HPLC, and the fraction with the strongest antioxidant activity (S4) was obtained. The scavenging ability of DPPH, •OH, and O 2 − were 53.96% ± 0.86%, 31.30% ± 0.90%, and 52.73% ± 0.99%, respectively. However, it still had lower antioxidant capacity compared with VC. Seven peptide sequences, namely, SLSHLTVQ, SLAHVTVQ, LHALTLQ, SGILAPSPVL, SHLTVQ, VSAAAA, and SPAAF, were identified from S4. The presence of certain amino acids, such as Glycine, Leucine, Phenylalanine, and Proline , is considered to induce high antioxidant activity against lipid peroxidation. Therefore, the antioxidant peptide extracted from millet gliadin after HHP treatment may be used as a natural antioxidant component in functional food.
研究领域:
食品科学
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生物化学
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农业加工
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
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IF 4.9
Dan Huang,SuQiu Si,Zhuoming Chen,Binjie Xin
In this study, a novel nanofiber material possessing antibacterial and antioxidant properties was fabricated through coaxial electrospinning, utilizing polylactic acid (PLA), thymol (THY), and nano zinc oxide (ZnO) as the raw materials. THY and ZnO was used to endows the membranes with good antibacterial and antioxidant properties. The surface morphology of the PLA/Z@T nanofiber membrane was characterized using scanning electron microscopy (SEM). Additionally, the successful formation of the core-shell structure was verified through transmission electron microscopy (TEM), fluorescence microscopy, and Fourier transform infrared spectroscopy (FTIR). Additionally, both ZnO and THY exhibit strong antibacterial properties, and their synergistic effect significantly enhances the antibacterial activity of the composite nanofiber membranes against Staphylococcus aureus . The drug sustained release percentages within 24 h for PLA@T and PLA/Z@T were 15.91 % and 14.82 %, respectively. The addition of zinc oxide does not significantly affect the release efficiency of thymol. After continuous release for 288 h, these percentages increased to 73.35 % and 72.94 %, respectively. By assessing antioxidant activity through DPPH and ABTS assays, it was found that PLA/Z@T exhibited higher activity compared to the control group. Subsequently, the in vitro antibacterial performance was investigated, revealing an antibacterial rate higher than that of the control group, the antibacterial rates against Staphylococcus and Escherichia coli reached 99.75 % and 98.49 % respectively. In conclusion, the results demonstrate that PLA/Z@T nanofiber membranes possess excellent antibacterial efficiency, sustained antibacterial performance, and antioxidant properties, offering a novel strategy for antibacterial materials.
研究领域:
材料科学
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纳米技术
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生物医学工程
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药剂学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Wei Liu,Lin Wang,Zipei Zhang,Xiaolai Zhang,Jingcheng Hao
As the improvement of consciousness of environment, a considerable effort has been made to shift from petroleum-based plastics to biodegradable alternatives, especially in the food packaging industry. In this work, we develop an innovative food packaging film by encapsulating quercetin (Que) within β -cyclodextrin ( β -CD) to create β -CD-Que inclusion complexes, which are then dispersed in a matrix of tragacanth gum (TG) and carboxymethyl chitosan (CMCS) using a solution casting technique. β -CD-Que inclusion complexes were characterized using various methods, including 1 H NMR, 2D ROESY and phase solubility testing, to elucidate the interaction between Que and β -CD. ATR–IR, X-ray diffraction, and SEM were employed to confirm the uniform dispersion of β -CD-Que within the macromolecular substrate. The incorporation of β -CD-Que considerably improves the mechanical properties of films, increasing the elongation at break from 13.68 % to 25.74 %. Rheological tests demonstrate that the viscosity of the film-forming complex solution became larger. The films were demonstrated to reduced water vapor and oxygen permeability, possessing remarkable antioxidant capability evidenced by DPPH and ABTS radical scavenging rates of 78.01 % and 99.59 %, respectively. The TG/CMCS/ β -CD-Que composite film shows excellent potential for food packaging and preservation applications.
研究领域:
食品科学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Kangyu Zhao,Xin Long,Junle Li,Ying Wang,Ping Lan,Yong Wang
BACKGROUND The Maillard reaction involves the interaction of various amino acids and reducing sugars, resulting in food browning. It often produces appealing aromas and flavors. The complexities of the reaction are such that it can be challenging to identify the often numerous and frequently volatile products formed by it. In the present study, we sought to identify and evaluate an unusual product with anti-oxidant activity arising from a fructose-histidine Maillard reaction model. The anti-oxidant profile of this product was assessed by computational means. RESULTS The fructose-histidine Maillard reaction products (FH-MRPs) were generated by heating a 2:1 mixture of the sugar and the amino acid at 140 °C for 2 h. Chromatographically separable fractions, labelled DM-1 to DM-8, were obtained using silica gel as the stationary phase and dichloromethane/methanol (DCM/MeOH) mixtures as the mobile one. Fraction DM-5 exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and further bio-assay guided fractionation led to isolation and identification of 1-(1 H -imidazo[4,5- c ]pyridin-4-yl)ethenone (IMPE) as the active principal, the structure of which was established by nuclear magnetic resonance (NMR) spectroscopic and mass spectral techniques. A mechanism for the formation of IMPE from its precursors is proposed. Density functional theory (DFT) calculations suggest this novel heterocyclic compound exerts its anti-oxidant effects by interacting with DPPH and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. Essentially, IMPE was non-toxic below 300 ug mL −1 , showing a concentration-dependent free radical clearance capacity and reducing power within the 100–1000 μg mL −1 range, and moreover, exhibiting significant Fe 2+ chelating abilities wihin the 50-200 μg mL −1 range. CONCLUSION This study identified the unique FH-MRP, IMPE, and found that it acts as food antioxidant through the chelation of metal ions. © 2024 Society of Chemical Industry.
研究领域:
食品化学
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分析化学
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计算化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Complementary and Alternative Medicine
·
2013
·Vol. 13
·期 1
·1-11
·
IF 2.082
Wang Shan-Shan,Wang Dong-Mei,Pu Wen-Jun,Li Deng-Wu
Background Extracts from Potentilla species have been applied in traditional medicine and exhibit antioxidant, hypoglycemic, anti-inflammatory, antitumor and anti-ulcerogenic properties, but little has been known about the diversity of phytochemistry and pharmacology on this genus. This study investigated and compared the phytochemical profiles, antioxidant and antimicrobial activities of leaf extracts from three Potentilla species (Potentilla fruticosa, Potentilla glabra and Potentilla parvifolia) in order to discover new resources for lead structures and pharmaceutical products.Methods Chemical composition and content of six phenolic compounds were evaluated and determined by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau colourimetric method and sodium borohydride/chloranil-based method (SBC); Antioxidant activities were determined using DPPH, ABTS and FRAP assays; Antimicrobial properties were investigated by agar dilution and mycelial growth rate method.Results The results showed hyperoside was the predominant phenolic compound in three Potentilla species by RP-HPLC assay, with the content of 8.86 (P. fruticosa), 2.56 (P. glabra) and 2.68 mg/g (P. parvifolia), respectively. The highest content of total identified phenolic compounds (hyperoside, (+)-catechin, caffeic acid, ferulic acid, rutin and ellagic acid) was observed in P. parvifolia (14.17 mg/g), follow by P. fruticosa (10.01 mg/g) and P. glabra (7.01 mg/g). P. fruticosa possessed the highest content of total phenolic (84.93 ± 0.50 mmol gallic acid equivalent/100 g) and total flavonoid (84.14 ± 0.03 mmol quercetin equivalent/100 g), which were in good correlation with its significant DPPHIC50 (16.87 μg/mL), ABTS (2763.48 μmol Trolox equivalent/g) and FRAP (1398.70 μmol Trolox equivalent/g) capacities. Furthermore, the effective methodology to distinguish the different species of Potentilla was also established by chromatographic fingerprint analysis for the first time. The results of antimicrobial activities showed P. fruticosa exhibited the strongest inhibition aganist Gram-positive bacteria, Pseudomonas aeruginosa and Candida albicans with MIC values of 0.78–6.25 mg/mL. P. parvifolia possessed antibacterial and antifungal activities against all the microorganisms tested, with EC50 and MIC values of 20.52–47.02 mg/mL and 0.78–50 mg/mL, respectively.Conclusion sThese results indicated that leaf extracts from three Potentilla species could become useful supplement for pharmaceutical products as a new antioxidant and antimicrobial agents.
研究领域:
药用植物学
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植物化学
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药学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2015
·
IF 2.416
Cunshan Zhou,Xiaojie Yu,Xiaopei Qin,Haile Ma,Abu ElGasim A. Yagoub,Jiali Hu
Kinetics modelling, DPPH radical scavenging activity and reducing power of rapeseed meal protein were investigated under simulated duodenum digestion. Kinetics of the reaction was considered in relation to initial substrate concentration, initial enzyme concentration, and hydrolysis time. Optimum hydrolysis conditions were trypsin concentration of 10 g/L, substrate concentration of 4 g/L, time of 40 min and temperature of 37 °C. From the results a general kinetic enzymolysis equation was suggested, providing a rational theoretical basis for determining the parameters of the reaction. The highest antioxidant activity was in accordance with the optimum reaction conditions. Antioxidant free amino acids increased after hydrolysis. Antioxidant oligopeptides of molecular weights of 2608, 1695 and 211 Da were identified. At a concentration of 2 mg/mL, the peptide fraction of CS-F 2 -CC 2 ; with molecular weight of 1000–1500 Da; showed the highest DPPH radical scavenging activity and reducing power, accounting for 70.32% and 0.538, respectively. The trypsin-hydrolysate displayed a potential capacity to scavenge free radicals and binding irons after duodenal digestion, appearing as promising ingredient to formulate functional foods with antioxidant activity.
研究领域:
食品科学
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生物化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2011
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IF 2.502
Jianbo Xiao,Jianglei Huo,Huixian Jiang,Fan Yang
The chemical compositions and bioactivities of crude tea polysaccharides (TPS) from the out-of-date tea leaves (beyond their useful date), namely Xihu Longjing (XTPS), Anxi Tieguanyin (TTPS), Chawentianxia (CTPS) and Huizhoulvcha (HTPS), in market were investigated. These TPS showed similar neutral sugar content and different distribution of molecular weight (1–800 kD). These crude TPS were mainly composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, and galacturonic acid. IR spectra confirmed that these crude TPS were composed of polysaccharide, protein and uronic acids. These TPS showed similar DPPH scavenging activity and exhibited lower DPPH scavenging activities than Vc within 25–200 μg/mL. However, these TPS with higher concentrations (200–400 μg/mL) showed similar DPPH scavenging activity with Vc. HTPS exhibited significant higher superoxide anion scavenging activity than others TPS and gallic acid. XTPS showed significant higher inhibitory effects on α-glucosidase and α-amylase with inhibitory percentages of 64.35% and 82.24% than others TPS. TTPS, XTPS, and HTPS exhibited similar inhibition ability on α- d -glucosidase and α-amylase. The overdue tea leaves can be a resource of tea polysaccharides as function food.
研究领域:
食品科学
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营养学
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药理学
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农业化学
✓ 已验证
DOI
PubMed
高影响因子
CyTA-Journal of Food
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2013
·
IF 0.5
Xin-Huai Zhao,Yu Fu,Nan Yue
Casein hydrolysate of a degree of hydrolysis of 9.4% was generated from caseinate using papain and modified by plastein reaction with extrinsic phenylalanine or tyrosine addition, and then fractionated by an ethanol-water solvent (6:4, v/v). Compared to casein hydrolysate or mixtures of casein hydrolysate and phenylalanine or tyrosine, two modified hydrolysates prepared thereof showed higher scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) or hydroxyl radicals and reducing power. The obtained soluble and precipitated fractions had higher and lower total hydrophobic amino acids especially stronger and weaker three antioxidant properties than the parent substrates, respectively. All hydrolysates particularly soluble fractions protected rat hepatocytes against H2O2- or galactosamine-induced injury in vitro, resulting in enhanced cell viability, decreased cellular malondialdehyde content, and lactate dehydrogenase leakage. Cytoprotection of the hydrolysates was observed at a dosage-dependence manner. A negative correlation (P < 0.05) was found between malondialdehyde content and scavenging activity on hydroxyl radicals or reducing power of the hydrolysates.
研究领域:
食品科学
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生物化学
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药理学
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细胞生物学
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药物化学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xiaoli Zhou,Yumeng Qin,Yanli Wang,Yaping Wang,Zhicheng Qin
Rosa roxburghii Tratt (RRT) is renowned for its abundance of phenolics, various methods have been used to extract free phenolics from RRT, there remains a paucity of research on the comparative distinctions between bound phenolic compounds and their free counterparts in RRT, especially in vitro and in vivo antioxidant potential of RRT-BP necessitates further exploration. This study sought to optimize the process parameters for extracting bound polyphenols through alkaline hydrolysis . Among the bound phenolic compounds, 20 phenolic acids and 34 flavonoids were identified, while the free phenolic compounds comprised 19 phenolic acids and 42 flavonoids. The IC 50 values for DPPH and ABTS radical scavenging were 44.92 μg/mL and 60.92 μg/mL for free phenols , 87.02 μg/mL and 95.02 μg/mL for bound phenols, respectively. Compared with the control group, the lifespan of Caenorhabditis elegans fed with 1 mg/mL of free and bound phenol extended from 11.63 d to 16.77 d and 13.88 d, respectively, with no adverse impact on the reproductive capability of the nematodes. The longevity of C. elegans was observed to persist even under thermal and oxidative stress , primarily attributed to elevated superoxide dismutase and catalase activity, coupled with reduced malondialdehyde content in C. elegans . These findings underscore that free polyphenols exhibit superior polyphenolic components and antioxidant properties compared to their bound counterparts. Nevertheless, the potential health effects of bound polyphenols as a supplement to free polyphenols in RRT should not be ignored.
研究领域:
药理学
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生物化学
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分子生物学
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营养学
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生物技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）