MOLECULES
·
2026
·Vol. 31
·期 12
·1990
·
IF 5.1
Jinghan Zhang,Yue Sun,Jinhao Zheng,Can Yang,Mingshuo Yang,Liming Pan
Dendrobium officinalehas attracted increasing attention as a functional food because of its diverse biological activities; however, the photoprotective potential of its protein-derived peptides remains poorly understood. In this study,D. officinaleprotein hydrolysates were fractionated by ultrafiltration according to molecular weight, and their protective effects against ultraviolet B (UVB)-induced photoaging were systematically evaluated in HaCaT keratinocytes. Among the tested fractions, low-molecular-weight peptide fractions exhibited relatively stronger antioxidant activity and effectively reduced intracellular reactive oxygen species (ROS) accumulation in UVB-irradiated cells. In addition, the peptide fractions alleviated UVB-induced inflammatory responses and decreased matrix metalloproteinase (MMP) expression, which was associated with modulation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways. Higher-molecular-weight fractions showed relatively stronger effects on maintaining skin barrier-related functions and were associated with regulation of transforming growth factor-β/Smad (TGF-β/Smad) signaling and collagen-related protein expression. Overall, these findings demonstrate functional differences amongDendrobium officinalepeptide fractions and suggest their potential application as natural photoprotective ingredients in functional foods and cosmeceutical products.
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DOI
PubMed
高影响因子
Plants-Basel
·
2026
·
IF 4.7
Hexigeduleng Bao,Yu Wang,Hainan Bao,Feijuan Wang,Qiong Jiang,Hua Li,Yanfei Ding,Cheng Zhu
With increasing industrialization, heavy metal contamination poses a growing threat to ecosystem stability and human health. Cadmium (Cd) is taken up by medicinal plants through the soil–plant continuum, altering rhizosphere microbial communities and thereby affecting plant metabolism and bioactive compound accumulation. In this study,Dendrobium officinaleKimura & Migo (D. officinale) was used as a model medicinal plant to investigate the effects of Cd exposure on plant quality and rhizosphere microbial community composition. The results show that differentD. officinalestrains exhibited significant differences in growth traits and Cd accumulation under Cd exposure. Under the 0.9 mg·kg−1Cd treatment, mean Cd concentrations in the strain with the highest Cd accumulation (Strain 2) reached 6.42, 1.08, and 1.70 mg·kg−1DW in roots, stems, and leaves, respectively, compared with 2.13, 0.73, and 0.66 mg·kg−1DW in the strain with the lowest Cd accumulation (Strain 1), corresponding to approximately 3.0-, 1.5-, and 2.6-fold higher accumulation. Cd exposure was associated with strain-specific changes in metal transport-related gene expression, antioxidant responses, bioactive constituent accumulation, and rhizosphere microbial community composition. Together, these changes highlight the complex relationship between Cd accumulation, plant physiological responses, and medicinal quality. These findings provide a theoretical reference for the selection of eliteD. officinalestrains and for the safe cultivation of this medicinal plant.
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Journal of Analytical Methods in Chemistry
·
2025
·Vol. 2025
·期 1
·8995925
·
IF 2.2
Zejun Wang,Qingcheng Wang,Fei Xu,Linhua Wang,Jingjing Liang,Qing Shen,Shunyuan Guo
This study developed a method for simultaneously detecting 10 mycotoxins in Dendrobium officinale using the QuEChERS technique combined with ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). The method was optimized for sample purification, pretreatment, chromatographic conditions, and mass spectrometric settings. It effectively addressed the matrix effects from impurities like pigments and cellulose. The validation of the method showed good linearity ( R 2 > 0.990), with limits of detection (LODs) ranging from 0.23 to 8.61 μg kg −1 and limits of quantification (LOQs) from 0.77 to 28.7 μg kg −1 . Average recoveries for the 10 mycotoxins ranged from 77.9% to 98.5%, with relative standard deviations (RSD) between 2.26% and 8.28%. The method demonstrated high accuracy, precision, and suitability for large-scale screening of mycotoxins in Dendrobium officinale. When applied to 84 samples, the contamination rate was 2.38%, with the main contaminants being AFB1, ZEN, and AFG1. This method provides a reliable, cost-effective approach for detecting mycotoxin contamination in traditional Chinese medicine.
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Yuan Li,Tong Gao,Ziying Wang,Mengyuan Wang,Xiao Liang,Wenxin Gao,Xiaodan Zhang,Mengni Li,Zongsuo Liang
Abstract Functional oligosaccharides have attracted attention as dietary interventions for type 2 diabetes mellitus (T2DM). However, the effects of oligosaccharides derived from Dendrobium officinale (DOO1) in T2DM remain unclear. This study evaluated the effects of DOO1 on metabolic parameters, gut microbiota, short-chain fatty acids (SCFAs) production, intestinal barrier function, and host metabolism in db/db mice. DOO1 significantly improved glucose and lipid metabolic disorders and reduced pancreatic and hepatic injury. These effects were accompanied by changes in gut microbiota composition, characterized by increased abundances of Duncaniella, Muribaculum, and Paramuribaculum, along with elevated fecal SCFA levels. DOO1 improved intestinal barrier function, as indicated by reduced intestinal permeability and inflammatory markers (TNF-α, IL-1β, LPS), and increased expression of Muc2, ZO-1, and Claudin-1. DOO1 also altered host metabolic profiles and regulated gut-derived hormones and appetite-related neuropeptides. Overall, these findings suggest that DOO1 may alleviate T2DM-related metabolic disorders by modulating gut microbiota and host metabolism.
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
·
IF 4.3
Zhang Shenao,Liu Pangwei,Jiang Yuge,Wu Huan,Cao Shijian,Xuan Shujuan,Wu Hongfei,Zhou An
Wilson’s disease (WD) is characterized by pathological copper accumulation, which drives hepatocellular oxidative stress and subsequent liver injury. While the first-line chelator penicillamine (PCA) effectively eliminates copper, it is associated with significant side effects. Dendrobium officinale polysaccharides, the active components in Dendrobium officinale , exhibit significant antioxidant and hepatoprotective effects. However, their potential in ameliorating liver injury in WD and as an adjunctive therapy to PCA remains unclear. This study investigated the hepatoprotective effects and underlying mechanisms of fresh Dendrobium officinale polysaccharides (FDOP), both alone and combined with PCA, in a copper-overloaded rat model. The results showed that FDOP significantly mitigated hepatic histopathological damage and reduced serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (AKP). It also alleviated oxidative stress by decreasing hepatic malondialdehyde (MDA) and oxidized glutathione (GSSG) levels while enhancing superoxide dismutase (SOD) activity. Furthermore, FDOP promoted copper excretion and reduced copper deposition in the liver, brain, and kidneys. Mechanistically, FDOP activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1)/Kelch-like ECH-associated protein 1 (Keap1) signaling pathway, evidenced by upregulated nuclear Nrf2 and HO-1 expression and downregulated Keap1 expression, thereby enhancing the cellular antioxidant defense system. Importantly, the combination of FDOP and PCA outperformed either monotherapy in alleviating liver injury, promoting copper excretion, and activating antioxidant pathways. In conclusion, FDOP not only exhibits excellent hepatoprotective effects but also holds potential as an adjunct to PCA therapy, offering a novel combined treatment strategy for WD.
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高影响因子
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