Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Chen Yu,Hengchang Zang,Cui Yang,Dong Liang,Shuang Quan,Danyang Li,Yanni Li,Qin Dong,Fengshan Wang,Lian Li
Osteoarthritis (OA) is a degenerative joint disease which is highly prevalent worldwide. However, no therapy for blocking OA pathogenesis is available currently. In this study, chondroitin sulfate (CS) E oligosaccharides were prepared and we identified disaccharide as the functional unit showing the strongest anti-complement activity and screened out complement C5 as its target in the complement system. We determined that CS-E disaccharide produced anti-inflammatory effects to treat OA by regulating the complement system: it inhibited the formation of complement-dependent complexes such as the membrane-attack complex (MAC) by targeting C5 and suppressed MAC-induced protein expression and the activation of downstream MAPK and NF-κB signaling pathways accordingly. By identifying CS-E disaccharide which could be regarded as a complement regulator or inhibitor exhibiting high anti-complement activity and revealing its OA-alleviating mechanism, this study not only provides a new strategy for OA treatment and drug development, but also potentially offers a promising C5 target therapy for other associated diseases.
研究领域:
药理学
·
免疫学
·
风湿病学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Zhao Huang,Li Zhou,Jiufei Duan,Siyuan Qin,Jingwen Jiang,Haining Chen,Kui Wang,Rui Liu,Minlan Yuan,Xiangdong Tang,Edouard C. Nice,Yuquan Wei,Wei Zhang,Canhua Huang
Loss of E-cadherin (ECAD) is required in tumor metastasis. Protein degradation of ECAD in response to oxidative stress is found in metastasis of hepatocellular carcinoma (HCC) and is independent of transcriptional repression as usually known. Mechanistically, protein kinase A (PKA) senses oxidative stress by redox modification in its β catalytic subunit (PRKACB) at Cys200 and Cys344. The activation of PKA kinase activity subsequently induces RNF25 phosphorylation at Ser450 to initiate RNF25-catalyzed degradation of ECAD. Functionally, RNF25 repression induces ECAD protein expression and inhibits HCC metastasis in vitro and in vivo. Altogether, these results indicate that RNF25 is a critical regulator of ECAD protein turnover, and PKA is a necessary redox sensor to enable this process. This study provides some mechanistic insight into how oxidative stress-induced ECAD degradation promotes tumor metastasis of HCC.
研究领域:
癌症生物学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
iScience
·
2023
·
IF 5.8
Yao Li,Zi-Yu Liang,Hai-Lin Wang
Summary Red blood cells supply the oxygen required for all human cells and are in demand for emerging blood-loss therapy. Here we identified N6-methyl-2′-deoxyadenosine (6mdA) as an agonist that promotes the hyperproliferation of burst-forming unit erythroid (BFU-E) progenitor cells. In addition, 6mdA represses the apoptosis of erythroid progenitor cells (EPCs). Combined use of with SCF and EPO enabled cultures of isolated BFU-E to be expanded up to 5,000-fold. Transcriptome analysis showed that 6mdA upregulates the expression of the EPC-associated factors c-Kit, Myb, and Gata2 and downregulates that of the erythroid maturation-related transcription factors Gata1, Spi1, and Klf1. Mechanistic studies suggested that 6mdA enhances and prolongs the activation of erythropoiesis-associated master gene c-Kit and its downstream signaling, leading to expansion and accumulation of EPCs. Collectively, we demonstrate that 6mdA can efficiently stimulate the EPC hyperproliferation and provide a new regenerative medicine recipe to improve ex vivo generation of red blood cells.
研究领域:
血液学
·
分子生物学
·
再生医学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Molecular Horticulture
·
2025
·Vol. 5
·期 1
·1-22
·
IF 8.1
Wang Zhiling,Yuan Yixin,Dong Rui,Zeng Ruihong,Zhao Xin,Xu Yanjie,Gao Junping,Hong Bo,Gu Zhaoyu
Floral fragrance is essential for the attraction of pollinators and responses to biotic and abiotic stresses. It also enhances the quality and economic value of plants. Phytohormones, acting as key signaling molecules, are crucial roles in regulating plant growth and development. However, the molecular mechanisms underlying the biosynthesis of fragrance-related volatiles and their crosstalk with other endogenous signals within plants remain largely unknown. Here, we identified a sesquiterpene synthase gene, CmEβFS , in chrysanthemum, which encodes a catalytic enzyme responsible for synthesizing the key fragrance-related compound ( E )-β-farnesene. We demonstrated that CmEβFS is negatively regulated by CmWRKY1, thereby inhibiting ( E )-β-farnesene biosynthesis. Furthermore, CmWRKY1 interacts with the salicylic acid (SA) receptor CmNPR3 to regulate SA-mediated CmEβFS transcription. Our findings reveal that SA regulates ( E )-β-farnesene biosynthesis by controlling CmEβFS expression via the CmWRKY1-CmNPR3 module during floral development. These findings enhanced our understanding of the mechanisms underlying SA-mediated regulation of volatile organic compounds (VOCs) biosynthesis and provided insights into improving floral fragrance.
研究领域:
植物学
·
植物生理学
·
分子生物学
·
生物化学
·
植物遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2015
·
IF 5.75
Menglin Wang,Jin Sun,Yinglei Zhai,He Lian,Cong Luo,Lin Li,Yuqian Du,Dong Zhang,Wenya Ding,Shuhong Qiu,Yuhai Liu,Longfa Kou,Xiangfei Han,Rongwu Xiang,Yongjun Wang,Zhonggui He
To improve the bioavailability of orally administered drugs, we synthesized a pH-sensitive polymer (poly(ethylene glycol)–poly(2-methyl-2-carboxyl-propylene carbonate)–vitamin E, mPEG–PCC–VE) attempting to integrate the advantages of enteric coating and P-glycoprotein (P-gp) inhibition. The aliphatic polycarbonate chain was functionalized with carboxyl groups and vitamin E via postpolymerization modification. Optimized by comparison and central composite design, mPEG113–PCC32–VE4 exhibited low critical micelle concentration of 1.7 × 10–6 mg/mL and high drug loading ability for tacrolimus (21.2% ± 2.7%, w/w). The pH-responsive profile was demonstrated by pH-dependent swelling and in vitro drug release. Less than 4.0% tacrolimus was released under simulated gastric fluid after 2.5 h, whereas an immediate release was observed under simulated intestinal fluid. The mPEG113–PCC32–VE4 micelles significantly increased the absorption of P-gp substrate tacrolimus in the whole intestine. The oral bioavailability of tacrolimus micelles was 6-fold higher than that of tacrolimus solution in rats. This enteric polymer therefore has the potential to become a useful nanoscale carrier for oral delivery of drugs.
研究领域:
药剂学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Hui Wang,Yanwen Shen,Ziyang Lou,Nanwen Zhu,Haiping Yuan,Chunnan Liu
Concentrated leachate is collected from two-stage anaerobic/aerobic-membrane bioreactor (MBR) and nanofiltration (NF) unit in landfill leachate treatment process and featured with non-biodegradation compounds and high salts content. E + -ozonation process was developed and introduced to deal with concentrated leachate, to make good use of chlorine ions (Cl − ) and the associated O 2 during ozonation process. 74.5% and 69.7% of COD and TOC were removed in E + -ozonation under the optimum conditions with initial pH of 9 and current density of 30 mA∙cm −2 , and the corresponding enhancement factors were 1.36 and 1.13, compared to the sum of the electrolysis and ozonation individual. The co-generation of reactive oxygen species (ROS) and reactive chlorine species (RCS) made a complementary effect on the degradation of refractory substances presented in concentrated leachate. 10.1% of total COD removal was improved by non-radical RCS generated in situ and 6.5% of total COD removal was achieved by ● OH-enhanced and Cl /ClO -emerged. With the help of electrolysis, the OH quencher of Cl − was converted into non-radical RCS (such as Cl − , ClO − and Cl 2 ) at anode, and the un-reacted O 2 /O 3 was reduced into OH at the cathode simultaneously. E + -ozonation offers a synergetic degradation potential for special wastewater with refractory matters and high Cl − content.
研究领域:
环境科学
·
废水处理
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MICROENCAPSULATION
·
2023
·
IF 3.9
Ran Tao,Qianyu Zhang,Jialing Duan,Ran Chen,Liyun Yao,Ruiteng Zhang,Gang Dong,Huali Chen
Aims The aim of this study is to develop a liposome that could exert unparalleled antioxidant effects. In the present study, a vitamin C (VC)/vitamin E (VE)-co-loaded multivesicular liposome (VCVE-MVL) was constructed.Methods Vitamins were encapsulated in soybean phosphatidylcholine (SPC) and cholesterol (CHO) by multi-emulsification method. The concentration of VC was determined by Fast Blue method. The concentration of VE was determined by high performance liquid chromatography (HPLC). Vitamin antioxidant capacity in vitro and in vivo was determined using β-carotene bleaching.Results VCVE-MVL with particle diameter of 848.55 ± 0.29 nm and SPAN of 0.16 ± 0.11 were obtained. The encapsulation efficiency of VC reached 48.51% (w/w)±0.15. Compared with VC/VE solution, VCVE-MVL had a higher permeation efficiency. In addition, the in vitro and ex-vivo antioxidant tests verified the adequate antioxidant activity of VCVE-MVL.Conclusions In conclusion, the antioxidant synergistic system we constructed and demonstrated its potential applications in the cosmetics industry.
研究领域:
药剂学
·
药学
·
化妆品行业
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Mohamed Eid,Jingsong Zhu,Muhammad Asif Ismail,Bin Li
Chemically cross-linked hydrogel nanoparticles (HGNPs) offer enhanced properties over their physical counterparts, particularly in drug delivery and cell encapsulation. This study applied pH-thermal dual responsive bio-adhesive HGNPs for dual complexation and enhanced the controlled release and bioavailability of cisplatin (CDDP) and Vitamin E (VE) drugs. The CDDP was loaded into the HGNPs via chemical conjugation with the carboxyl groups in the HGNPs surface by soy polysaccharides (SSPS). At the same time, the host-guest interaction complexed the VE through the β-cyclodextrin (β-CD). The HGNPs showed a uniform HGNPs size distribution of 90.77 ± 14.77 nm and 81.425 ± 13.21 nm before and after complexation, respectively. The FTIR, XRD , XPS , and zeta potential confirmed the conjugation. The cumulative release percent of CDDP reached 98 % at pH 1.2, while <45 % was released at pH 7.4. Our HGNPs enhance the incorporation of CDDP by substituting its chlorides with carboxyl groups of the SSPS; the loading of CDDP and VE was 15 ± 0.33 and 11.32 ± 0.25 wt%, respectively. Moreover, the CDDP and VE also released slower from the HGNPs at 25 °C than at 37 °C and 42 °C. The (VE/CDDP)-loaded HGNPs exhibited longer circulation time in vivo than free CDDP and free VE suspension.
研究领域:
药学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Yongkang Xi,Yuxiao Zou,Zhigang Luo,Liang Qi,Xuanxuan Lu
Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals. However, how to accurately control their in vivo release and stability is still challenging. Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE). The resulting dual-functional particle presented a uniform sheetlike shape and nanometer size. In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Benefiting from the antioxygenation of VE, lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion was effectively inhibited. Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions. In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment. This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.
研究领域:
药剂学
·
营养学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Xiaoli Tan,Xiaobing Jiang,Okwong Oketch Reymick,Chen Zhu,Nengguo Tao
Stem-end rot caused by Neofusicoccum parvum is among the most detrimental diseases affecting postharvest mangoes. The present investigation utilized ( E )-2-octenal to manage N. parvum infections, elucidating its mechanism of action. The findings revealed that ( E )-2-octenal exhibited outstanding antimicrobial potency against N. parvum in vitro within the concentration range of 0.4–1.6 µL/mL. Additionally, ( E )-2-octenal significantly compromised the cell membrane integrity and mitochondrial energy metabolism of N. parvum , evidenced by dramatically increased intracellular material leakage and ROS levels, along with reduced mitochondrial membrane potential, ATP, and energy charge. Further experiments showed noteworthy effects on the tricarboxylic acid cycle (TCA) cycle and the key enzyme activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH). Molecular docking revealed hydrogen bonding between ( E )-2-octenal’s aldehyde group and SDH (Trp-307) and MDH (Gly-101), indicating direct targeting of these enzymes for inhibition. To enhance the practical application of ( E )-2-octenal, we developed an aerogel-loaded ( E )-2-octenal material (ALO) that exhibited superior antimicrobial efficacy in vitro . In vivo , ALO effectively controlled mango stem-end rot, with optimal efficacy at 20 µL/L. This concentration also delayed the natural disease of mango without degrading fruit quality. According to these findings, ( E )-2-octenal is a promising preservative against postharvest mango infections, potentially by impeding cellular energy metabolism through direct interaction with SDH and MDH within the TCA pathway, culminating in mitochondrial dysfunction and cell membrane damage.
研究领域:
植物病理学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Yanjun Sun,Xingdan Wang,Qiming Wei,Xiaopo Wang
Owing to zero ozone depletion potential (ODP) and extremely low global warming potential (GWP), 2,3,3,3-tetrafluoroprop-1-ene (R1234yf) and trans-1,3,3,3-tetrafluoropropene (R1234ze(E)) as most potential refrigerants have attracted much interest in refrigeration and air conditioning (RAC). For a better insight into the refrigeration performance using R1234y and R1234ze(E) as refrigerants, the thermophysical properties of refrigerant/lubricant blends should be well understood, especially the viscosities of the refrigerant/lubricant blends. Using a self-designed-capillary apparatus, the viscosity measurements were performed for blends of POE85 oil saturated with R1234y and R1234ze(E) between 303.15 and 343.15 K. Viscosities of both R1234yf/POE85 and R1234ze(E)/POE85 have a similar trend. With an increase in solubility, the viscosity plunges dramatically at the beginning and then drops slowly. The results indicate that the refrigerant dissolved in oil has a large impact on the lubricant viscosity. The experimental data were successfully correlated by Eyring’s absolute rate theory coupled with modified two-suffix-Margules (Eyring-MTSM model).
研究领域:
热力学
·
制冷工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Materials Research Express
·
2021
·Vol. 8
·期 12
·125006
·
IF 1.62
Peng She,Chun Yao,Jun Li,Hegang Bao,Xiuquan Xu,Hong Zhou
Co-doping is one of the most facile and efficient strategies for boosting the photocatalytic performance of g-C3N4. Herein, a series of Ti and O co-doped g-C3N4 (Ti/OCN) composites with excellent photocatalytic inactivation activity of Enterobacter cloacae (E. cloacae) were successfully synthesized by simple calcination of urea/formic acid/TiCl4 precursor. The obtained Ti/OCN-3 possessed the optimal photocatalytic capability with complete inactivation of 6.20 log CFU·ml−1 E. cloacae under 60 min of visible light irradiation. The improved photocatalytic disinfection performance was probably attributed to the synergistic effect of Ti and O co-doping, which led to an extended visible light response and accelerated charge transport efficiency. Furthermore, scavenger trapping and electron spin resonance (ESR) experiments revealed that ·O2−, h+ and ·OH were responsible for the enhanced inactivation activity. Overall, this research provided a promising strategy for fabricating g-C3N4-based catalysts to efficiently deal with the hazards of pathogenic microorganisms.
研究领域:
环境科学
·
微生物学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）