INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Weiqin Wang,Xuefeng Li,Fanli Shi,Zhenhai Zhang,Huixia Lv
In this study, γ-cyclodextrins (γ-CD) and epigallocatechin-3-gallate (EGCG) were designed to form an inclusion complex (EGCG-γ-IC) for ulcerative colitis (UC) treatment. The drug-excipient combined therapeutic potential of γ-CD and EGCG was verified, when stability and compliance were also achieved. EGCG-γ-IC effectively inhibited the secretions of NO, TNF-α, and IL-6 and the intracellular ROS in RAW264.7 cells. The effectiveness of EGCG-γ-IC in treating DSS-induced acute UC in mice was observed including improving the histological conditions of the colon, reducing the levels of IL-1β, IL-6, and TNF-α in serum, and restoring MPO , GSH, and sIgA levels in intestinal tissues. Moreover, EGCG-γ-IC had a more prominent effect on regulating bacterial dysbiosis caused by DSS than EGCG and γ-CD alone. Therefore, EGCG-γ-IC designed here displayed UC treating capacity with safety in the long-term application and promised an industrial production potential due to its excellent storage stability.
研究领域:
药学
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炎症性肠病
·
药物递送系统
·
免疫学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
ChuanYao Zhai,WenHao Wang,AnQuan Ma,HuanYu Liu,TianTian Zhu,Yan Zhang,TianJiao Yu,ZiQing Yang,Jing Lan,ZhiFeng Wang
Guided bone regeneration (GBR) is a prominent focus in biomedical materials research, yet few studies address practical clinical needs. GBR membranes must fulfill the “PASS” principles to be effective in surgery, but existing membranes often fall short in balancing antibacterial activity, controlled degradation, osteoinductive potential, and mechanical support. In this study, we employed laser powder bed fusion (LPBF) to fabricate a porous WE43 magnesium alloy scaffold suitable for large alveolar bone defects. We then used electrospinning and layer-by-layer (LBL) self-assembly to create a silk fibroin-based barrier membrane loaded with epigallocatechin gallate (EGCG) and polydopamine (PDA) for combined antibacterial and osteogenic functions. Laboratory tests confirmed that the WE43-F scaffolds and the EGCG/PDA-loaded membranes (SFE) are both biocompatible and bioresorbable. In vitro, they enhanced new bone formation by upregulating alkaline phosphatase (ALP) and RUNX2 gene expression while effectively inhibiting Escherichia coli and Staphylococcus aureus. In a rat maxillary defect model, the WE43-F/SFE composite system demonstrated superior bone regeneration, yielding the largest area of new bone and the most mature collagen–bone matrix, with a bone volume to total volume ratio (BV/TV) of 28.06%. In this study, we assembled WE43-F scaffolds with PDA-EGCG electrospun membranes to create a biodegradable GBR system that delivers synergistic antibacterial and osteogenic effects. Its antimicrobial efficacy and bone-regenerative capacity were confirmed through in vitro assays, cell culture experiments, antibacterial testing, and a rat mandibular defect model.
研究领域:
生物医学工程
·
材料科学
·
组织工程
·
骨科
·
牙科材料
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Yijia Liu,Yuanjie Wu,Jieyan Zhang,Haoren Wu,Zhibing Zheng,Chaoxi Zeng,Xinguo Liu,Xiaohua Li,Jianhui Ye,Zhonghua Liu,Meng Shi
Bovine serum albumin (BSA)-epigallocatechin-3-gallate (EGCG) have been widely studied; however, the effects of EGCG concentration on the properties of these complexes and their influence on the stability of high-internal-phase Pickering emulsions (HIPPEs) remain limited. This study aims to reveal the interactions between BSA and varying concentrations of EGCG and the application of BSA-EGCG complexes for creating stable emulsions. As the concentration of EGCG decreases from 0.3% to 0.1%, the β-sheet content significantly increased and the β-turn content significantly decreased, resulting in smaller sizes and zeta potentials of 50 μm and −58.7 mV, respectively, at a 0.1% EGCG concentration. This change enhances the ability of BSA-EGCG to adsorb at the oil-water interface, effectively lowering interfacial tension and increasing the emulsion viscosity. Moreover, the interactions between BSA and EGCG markedly improved pH stability, ionic stability, thermal stability, centrifugal stability, and freeze-thaw stability of emulsions. These findings highlight the valuable role of EGCG levels on BSA-EGCG interactions and formed HIPPEs, suggesting their promising applications in the food and pharmaceutical industries.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 14
·4361
·
IF 4.412
Ya Wang,Xiao-Jing Shen,Fa-Wu Su,Yin-Rong Xie,Li-Xia Wang,Ning Zhang,Yi-Long Wu,Yun Niu,Dong-Ying Zhang,Cheng-Ting Zi,Xuan-Jun Wang,Jun Sheng
Lung cancer is one of the most commonly occurring cancer mortality worldwide. The epidermal growth factor receptor (EGFR) plays an important role in cellular functions and has become the new promising target. Natural products and their derivatives with various structures, unique biological activities, and specific selectivity have served as lead compounds for EGFR. D-glucose and EGCG were used as starting materials. A series of glucoside derivatives of EGCG (7–12) were synthesized and evaluated for their in vitro anticancer activity against five human cancer cell lines, including HL-60, SMMC-7721, A-549, MCF-7, and SW480. In addition, we investigated the structure-activity relationship and physicochemical property–activity relationship of EGCG derivatives. Compounds11and12showed better growth inhibition than others in four cancer cell lines (HL-60, SMMC-7721, A-549, and MCF), with IC50values in the range of 22.90–37.87 μM. Compounds11and12decreased phosphorylation of EGFR and downstream signaling protein, which also have more hydrophobic interactions than EGCG by docking study. The most active compounds11and12, both having perbutyrylated glucose residue, we found that perbutyrylation of the glucose residue leads to increased cytotoxic activity and suggested that their potential as anticancer agents for further development.Keywords:EGCG;synthesis;cytotoxicity;EGFR;molecular docking
研究领域:
药理学
·
肿瘤学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2020
·
IF 3.067
Aijing He,Xiao Guan,Hongdong Song,Sen Li,Kai Huang
(−)-epigallocatechin-gallate (EGCG), a biologically active compound in green tea, has been found to have antimicrobial, anti-carcinogenic, and anti-oxidative activities, but it can easily be degraded when exposed to light and high temperature. A liquid-liquid dispersion method was used to prepare EGCG-hordein nanoparticles to better protect EGCG from degradation. The effects of different processing variables on the storage stability and interaction mechanisms between EGCG and hordein from those nanoparticles were investigated. Results showed that the solution pH was the most significant processing factor in terms of determining the stability of EGCG-hordein nanoparticles. Specifically, the EGCG-hordein nanoparticles showed an average particle size of 160 ± 10 nm, Zeta potential of 20.7 ± 0.4 mV, encapsulation efficiency of 91.2%, and loading efficiency of 15.2%. EGCG-hordein nanoparticles had better storage stability at 4 °C. The fluorescence quenching mechanism between EGCG and hordein was found to be due to static quenching and Förster non-radiative energy transfer.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Chenlu Ma,Youfa Xie,Xin Huang,Lu Zhang,David Julian McClements,Liqiang Zou,Wei Liu
A novel phospholipid-based nanovesicle (PBN) was developed to encapsulate (-)-epigallocatechin gallate (EGCG), a major polyphenol in green tea, to mask its bitter taste and expand its application in food products. The PBN was formed using W/O emulsion-transfer methods and showed a multilayer membrane nanovesicle structure (around 200 nm) observed with TEM. The PBN possessed a high encapsulation efficiency (92.1%) for EGCG. The bitterness of EGCG was significantly reduced to 1/12 after encapsulation. Fourier transform infrared spectroscopy (FTIR) indicated the EGCG mainly interacted with the upper chain/glycerol/head group region of the lipid bilayer in PBN. Quartz crystal microbalance with dissipation (QCM-D) showed the addition of γ-cyclodextrin in PBN enhanced EGCG's adsorption with phospholipids and allowed for its good sustained release. Encapsulating EGCG in PBN inhibited its complexation with mucin, reducing bitterness and astringency. This provides a new method to improve EGCG's flavor, potentially expanding its application in the food industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 9
·1273
·
IF 5.561
Jie-Qiong Wang,Ying Gao,Dan Long,Jun-Feng Yin,Liang Zeng,Yan-Qun Xu,Yong-Quan Xu
Hydrogen peroxide has a significant effect on the flavor of tea beverages. In this study, the yield of hydrogen peroxide in (–)-epigallocatechin gallate (EGCG) solution was first investigated and found to be significantly enhanced under specific conditions, and the above phenomenon was amplified by the addition of linalool. Then, an aqueous hydrogen peroxide solution was added to a linalool solution and it was found that the concentration of linalool was significantly reduced in the above-reconstituted system. These findings were verified by extending the study system to the whole green tea infusions. The results suggested that the production of hydrogen peroxide in tea beverages may be dominated by catechins, with multiple factors acting synergistically, thereby leading to aroma deterioration and affecting the quality of tea beverages. The above results provided a feasible explanation for the deterioration of flavor quality of green tea beverages with shelf life.Keywords:green tea beverages;EGCG;hydrogen peroxide;linalool;flavor
研究领域:
食品科学
·
化学
·
饮料技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 17
·6344
·
IF 4.6
Shuanghui Wang,Chuhong Chen,Yan Lu
Cadmium (Cd), a harmful heavy metal that has no biological purpose, can harm healthy fetal and child development. Epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in tea, has been shown to increase cell viability under Cd exposure and ameliorate Cd-induced kidney injury in adult male rats. Using theCaenorhabditis elegans(C. elegans) model, we demonstrated that EGCG mitigated Cd-induced body size developmental toxicity through a mechanism that did not involve chelation with EGCG and was not associated with Cd accumulation and efflux. Our research indicated that the beneficial effects of EGCG on Cd-induced body size developmental toxicity were associated with the mitigation of endoplasmic reticulum stress. Furthermore, our observations indicate that EGCG reduced Cd-induced developmental toxicity inC. elegansvia the PEK-1/eIF-2α/ATF-4 pathway. Our results provide important evidence for the potential benefits of consuming tea as a detoxification agent.Keywords:tea;EGCG;cadmium;Caenorhabditis elegans;developmental toxicity;endoplasmic reticulum stress
研究领域:
毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Meng Shi,Dan-Yang Ying,Mya Myintzu Hlaing,Jian-Hui Ye,Luz Sanguansri,Mary Ann Augustin
Novel delivery systems for epigallocatechin gallate (EGCG) were developed using broccoli by-products and their fractions as carriers. Puree and pomace from broccoli by-products had higher adsorption capacities for EGCG than juice at 25 °C (43.20 mg g −1 , 39.47 mg g −1 and 25.22 mg g −1 dry weight for pomace, puree and juice respectively). Chemical sorption is the rate-controlling step for EGCG-broccoli interactions. Langmuir and Freundlich models well described the adsorption of EGCG onto puree and pomace. FTIR results indicated that EGCG-puree had stronger interaction than EGCG-pomace. When the same level of EGCG (∼26 mg) was added to different matrices, more EGCG (∼20%) was recovered from the in vitro digestion system of EGCG-loaded puree than from the EGCG-loaded pomace (14%) and neat EGCG (9%). The antioxidant capacity of the whole digesta was positively correlated with the EGCG levels. Broccoli by-products are promising carriers for delivering and stabilizing EGCG through gastrointestinal digestion.
研究领域:
食品科学
·
营养学
·
天然产物化学
·
药物递送
·
生物利用度
·
分子互作
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2012
·
IF 3.655
Yali Wang,Yu Zhao,K. Andrae-Marobela,H. Okatch,Jianbo Xiao
The crude polysaccharides from tea leaves (TPS) looks like have good antioxidant activities. However, whether or not the antioxidant abilities of crude TPS depend on tea polyphenols in the crude TPS is not understand. Herein, TPS fractions were isolated from crude TPS and their antioxidant activities were compared. The crude TPS showed stronger antioxidant activities than that of TPS fractions. However, EGCG (6.15 μg/mL) significantly enhanced DPPH radical scavenging potential of TPS fractions for each dose. Moreover, EGCG (8.0 μg/mL) significantly improved the reducing power of TPS fractions. EGCG causes a synergistic increase in the antioxidant activities of TPS fractions. The antioxidant activities of dextrans in the absence and presence of EGCG were investigated to illustrate that the antioxidant activities of TPS is similar to dextrans. These results illustrated that TPS fractions hardly exhibited antioxidant activities and tea polyphenols are the major antioxidant in the crude TPS. TPS as food antioxidants is an old woman’s tale.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Caiying Zhang,Yuelong Li,Abdus Samad,Hongliang He,Huan Ma,Yang Chen,Tengchuan Jin
Kyasanur Forest disease virus (KFDV), a tick-borne flavivirus prevalent in India, presents a serious threat to human health. KFDV NS3 helicase (NS3hel) is considered a potential drug target due to its involvement in the viral replication complex. Here, we resolved the crystal structures of KFDV NS3hel apo and its complex with three phosphate molecules, which indicates a conformational switch during ATP hydrolysis . Our data revealed that KFDV NS3hel has a higher binding affinity for dsRNA , and its intrinsic ATPase activity was enhanced by dsRNA while being inhibited by DNA . Through mutagenesis analysis, several residues within motifs I, Ia, III, V, and VI were identified to be crucial for NS3hel ATPase activity. Notably, the M419A mutation drastically reduced NS3hel ATPase activity. We propose that the methionine-aromatic interaction between residues M419 and W294, located on the surface of the RNA-binding channel, could be a target for the design of efficient inhibitor probes. Moreover, epigallocatechin gallate (EGCG), a tea-derived polyphenol, strongly inhibited NS3hel ATPase activity with an IC 50 value of 0.8 μM. Our computational docking data show that EGCG binds at the predicted druggable hotspots of NS3hel. Overall, these findings contribute to the development and design of more effective and specific inhibitors.
研究领域:
病毒学
·
结构生物学
·
药物设计
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2019
·
IF 7.149
Jinxiu Lou,Wei Wang,Lizhong Zhu
Halobenzoquinones (HBQs) are frequently detected disinfection byproducts (DBPs) in drinking water with high toxicity and relevance to public health. In this study, we characterized the occurrence, formation, and oxidative stress of the HBQs in tea. 2,6-DCBQ and TetraC-1,2-BQ were identified in all prepared teas at total concentrations of 1.3–2.0 ng/L. 2,6-DCBQ originated from drinking water DBPs, while TetraC-1,2-BQ originated from tea leaves or were generated during tea polyphenol chlorination. HBQs in tea induced the formation of reactive oxygen species and semiquinone radicals, and the oxidative stress could be depleted by tea polyphenols, e.g., (−)-epigallocatechin gallate (EGCG). High-resolution mass spectrometry analysis indicated that the HBQs combined with EGCG and formed adducts at a ratio of 1:1 or 2:1 with the binding sites on the A ring and B ring of EGCG. The viability of HepG2 cells exposed to 50 μM 2,6-DCBQ was increased from 20.0% to 65.2% when 50 μM of EGCG was added. These results demonstrated that various HBQs can occur in tea due to the HBQ DBPs in drinking water, the leachate from tea leaves, and the chlorination of tea polyphenols; furthermore, the oxidative stress and cellular toxicity induced by HBQs in tea could be decreased by tea polyphenols. This is the first study to report HBQs in tea, elucidate the sources of HBQs, and assess relevant health risks.
研究领域:
环境科学
·
公共卫生
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）