Nanoscale
·
2023
·
IF 6.7
Jie Dai,Zhiyi Peng,Shuwei Shen,Binbin Huang,Lili Ren,Jia Liu,Chia Hung Chen,Guoguang Chen
The dysfunction of intracellular proteins is frequently associated with various diseases, such as cancer. The exogenous proteins in cells are usually assembled with specific configurations due to physiological confinement/crowding to exhibit novel features in protein structure, folding or conformational stability, distinguished with their behaviors in buffer solutions. Here, we synthesized exogenous proteins in a confined/crowded condition, to explore protein activity within the cells. The findings suggested that the confinement and crowding effects on protein activity are heterogeneous; they showed an inhibitory consequence on HRP by decreasing Km (from ~9.5- and ~21.7-fold) and Vmax (from ~6.8- and ~20.2-fold) lower than that of dilute solutions. Interestingly, the consequences on Cyt C seem more complicated, and the crowding exerts a positive effect by increasing Km ~3.6-fold and Vmax ~1.5-fold higher than that of dilute solutions; however, the confinement takes a negative consequence by decreasing Km ~2.0 and Vmax ~8.3 times. Additionally, in contrast to traditional nanoparticles-based confinement models, we synthesized a biodegradable nanoparticle to mimic the confined space, and the biggest advantage of this novel model is that the particles can be degraded and thus it can provide more intuitive observations of the target protein properties under the confinement and after release. Furthermore, we also evaluated protein activity in different cellular environments, indicating the exogenous protein activity was closely related to the crowdedness of cellular environments, and the inhibition of protein activity in MDA-MB-231 cancer cells was more obvious than HEK293 normal cells. Finally, SAXS analysis revealed the correlation between the protein conformation and the different environments. Our work will provide a unique way for precisely assessing whether the target cellular environments are native matrix in which specific exogenous protein drugs delivered to function or whether they display a therapeutic role, which is of great significance for screening and development of new drugs.
研究领域:
生物物理学
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细胞生物学
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药学
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纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Jianqiao Liu,Jinli Wang,Jiang Guo,Kai Yan,Jingdong Zhang
The coupling of photoelectrocatalysis (PEC) and enzymatic catalysis was explored for efficient treatment of practical phenol-containing coking wastewater outdoors. The developed enzymatic-photoelectrocatalytic (EPEC) system consisted of three-dimensional dual photoelectrodes fabricated with carbon fiber cloth (CFC), namely a CFC-supported WO 3 photoanode with oxygen vacancies and a CFC-supported Au-BiOBr photocathode with immobilized horseradish peroxidase (HRP). The experimental results and computational fluid dynamics study revealed that dual CFC-based photoelectrodes with three-dimensional network structures could optimize the fluid diffusion on the electrode surface and enhance the efficiency of pollutant degradation. Further investigation indicated that the introduction of oxygen vacancies promoted the PEC oxidation activity of the WO 3 photoanode while the Au nanoparticles modification was favorable to improving the immobilization of HRP and PEC generation of H 2 O 2 on BiOBr photocathode. Moreover, photovoltaic (PV) cells were integrated in the EPEC system to provide applied potential and promote the utilization of solar irradiation. The mineralization rate of coking wastewater reached 88.5% using the PV-integrated EPEC system driven by natural solar energy, which demonstrated potential for treating actual industrial wastewater.
研究领域:
环境科学
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电化学
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废水处理
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可再生能源
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催化
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 19
·9451-9458
·
IF 3.3
Xiujie Wang,Guoru Dai,Bianli Cheng,Jing Xu,Keming Xu,Wenying Zhong
Self-assembled peptide hydrogels are a class of versatile materials with inherent biocompatibility and sound biological activities; however, they tend to exhibit weak mechanical properties and unsatisfactory release profiles of drugs. To address these problems, we propose an enzymatic approach to synthesize an enzymatically cross-linked peptide hydrogel (Fmoc-Gly-Phe-Tyr-Tyr, or Fmoc-GFYY) on the basis of inter-cross-linking of tyrosines (Tyr) under the action of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Compared to Fmoc-GFYY without HRP or H2O2, the Fmoc-GFYY/enzymatically cross-linked hydrogels exhibited denser cross-linking networks, a more orderly secondary structure, and greater mechanical strength. Further characterizations using HPLC, UV, fluorescence, and LC-MS confirmed that the tyrosines of Fmoc-GFYY were inter-cross-linked to give dimeric tyrosines under the action of HRP and H2O2. By taking doxorubicin (DOX) and rhodamine B (RhB) as model drugs, the Fmoc-GFYY/enzymatically cross-linked hydrogel displayed slow release of DOX in vitro and a long retention time of RhB in vivo. Cell experiments revealed that the DOX-loaded enzymatically cross-linked hydrogel formulation exhibited higher anticancer efficacy compared to DOX solution alone. The utility of enzymatic cross-linking in this study represents a new approach for the structural optimization of peptide hydrogels, which could have broad prospects in the controlled release of various therapeutics.
研究领域:
材料科学
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药学
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生物医学工程
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药物递送系统
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DOI
高影响因子
MATERIALS & DESIGN
·
2021
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IF 7.991
Baolin Chen,Xun Wang,Xia Gao,Jie Jiang,Mancheng Hu,Shuni Li,Quanguo Zhai,Yucheng Jiang
In this work, TiO 2 nano particle was used for two purposes: it played as a role of a solid carrier for immobilization of HRP, and meanwhile acted as a photo-catalyst itself. In this way, the loss of enzymatic activity during enzyme immobilization can be compensated by the coupling of TiO 2 photo-catalysis. Then, the surface of TiO 2 nanoparticles were modified by phase-transitioned lysozyme (PTL) and connected with horseradish peroxidase (HRP) via a DNA helix ( [email protected] 2 ). The DNA double helix chain was utilized as a flexible spacer arm to keep the flexibility of the immobilized enzyme. The [email protected] 2 showed a high recovered activity (90.52%) of the original activity of free HRP. This data is better than most reported activity recovery of immobilized enzyme. Moreover, [email protected] 2 can maintain high catalytic activity, stability and reusability when degrading phenol in wastewater. The phenol of 0.5 mmol∙L −1 could be totally degraded within 10 min. Moreover, a visual colorimetric method for phenol concentration by [email protected] 2 was established, and the phenol concentration of 0–0.2 mmol∙L −1 can be visually detected with naked eyes. Furthermore, an enzymatic-photocatalytic synergetic effect of [email protected] 2 is found and investigated. The efficiency of [email protected] 2 ensures it a potential application in the wastewater treatment.
研究领域:
环境科学
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化学工程
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生物技术
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材料科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Chixuan Yao,Yaozhong Hu,Qisijing Liu,Jing-Min Liu,Xuemeng Ji,Huan Lv,Shuo Wang
To improve the performance of peanut allergen Ara h 3 detection, depending on boron and nitrogen carbon dots (B/ N -CDs), a nanobody (Nb) mediated dual-mode immunoassay was established, which combines the dominance of colorimetry with ratiometric fluorescence techniques. With the catalysis of Horseradish peroxidase (HRP), the oxidization of o-phenylenediamine (o-PD) in the presence of H 2 O 2 , leading to the production of yellow 2,3-diaminophenolazine (DAP) with an absorption peak at 431 nm. Owing to inner filter effect (IFE), DAP quenched the fluorescence of B/ N -CDs at 426 nm, and it emerged a new emission peak at 549 nm. The fluorescence intensity ratio and absorption intensity can be utilized for quantitative analysis of Ara h 3 concentration. Under optimal conditions, the detection limits were 6.61 and 9.79 ng·mL −1 , respectively. The dual-mode immunoassay was assessed containing specificity, stability, reproducibility, and practicability. This method paved the way for sensitive detection of Ara h 3 without background Interference.
研究领域:
过敏学
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分析化学
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纳米技术
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食品安全
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Lizhu Zeng,Chenjia Lin,Peiqing Liu,Duanping Sun,Jing Lu
Cardiac troponin I (cTnI), a principal biomarker of acute myocardial infarction, possesses superior cardiac specificity and can provide a long detection window for cardiac injury. Although the potential of cTnI as a cardiac biomarker is promising, sensitive and rapid detection of cTnI remains challenging. In this work, a novel colorimetric biosensor was developed for cTnI detection based on multifunctional hybrid nanoprobes coupled with triple-aptamer-modified tetrahedral DNA nanostructures (TAPT-TDNs). First, we evaluated the affinity of four TAPT-TDNs with three vertices modified by two kinds of aptamers in different proportions. The four TAPT-TDNs exhibited much better affinity than the single-stranded aptamer for cTnI, which was attributed to the fact that TAPT-TDNs could improve the capture efficiency of cTnI with excellent interface density and constant support. Second, the selected TAPT-TDNs with highest affinity were immobilized on Fe 3 O 4 -Au magnetic nanoparticles as capture probes (named Fe 3 O 4 -Au-TAPT-TDNs). TAPT-TDNs and HRP were both labeled on NH 2 -MIL-101(Fe) to form signaling probes (named MIL-101(Fe)-HRP-TAPT-TDNs). Then, with the help of capture probes and signaling probes, the target protein was recognized and captured to form capture probe-cTnI-signaling probe sandwich-like structures, causing a decrease in signaling probes in the supernatant with magnetic separation and eventually leading to a visual color change. This colorimetric aptasensor displays excellent analytical performance toward cTnI with a detection limit of 27 pg·mL −1 . The anisotropic aptamer-mediated colorimetric sensor may serve as an alternative to conventional immunoassays for quick and accurate detection of cTnI in clinical samples.
研究领域:
生物传感器
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纳米技术
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分析化学
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分子生物学
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心脏病学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Bin Cao,Mengyu Li,Ye Zhao,Huimin Zhou,Ting Tang,Mengran Li,Chuan Song,Wei Zhuang
In recent years, two-dimensional nanosheet metal-organic frameworks (2D MOFs) have been widely considered as promising carriers for enzyme immobilization owing to their large surface area, designable and tunable structures, and other properties that enhance enzyme loading and modulate interactions with enzymes. In this study, a series of ultrathin 2D M-TCPP (M = Co, Ni, Zn, Cu) nanosheets were synthesized employing a surfactant-assisted bottom-up approach, and the effect of solvent ratio on the morphology and properties of 2D MOFs was explored. After systematic characterization, Cu-based 2D MOFs with large specific surface areas and excellent water stability was selected as the carrier for the co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP). The effects of adsorption and covalent immobilization strategies on bis-enzyme loading and enzyme activity , as well as their applications in rapid glucose detection, were systematically investigated. The results showed that A-CTGH and C-CTGH owned enzyme loadings of 187.9 and 249.1 mg/g, respectively, and exhibited superior enzymatic activity when exposed to harsh environments compared to free enzymes. In addition, the covalently immobilized biocatalyst based on GOx demonstrated a more sensitive glucose detection performance, including a wide linear range from 5.0 to 16 μM with a detection limit of 0.6 μM.
研究领域:
纳米材料
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生物催化
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2020
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IF 10.257
Lizhen Chen,Jie Wu,Feng Yan,Huangxian Ju
This work designed a facile sensing strategy for quantitation of glycans on cell surface using organic electrochemical transistors (OECTs). The sensing strategy was performed by covalently binding target cells on mercaptopropionic acid modified gate electrode for the recognition of horseradish peroxidase (HRP) labeled lectins to specific glycans on cell surface, which led to sensitive channel current signal responding to the captured HRP and thus the expression of cell surface glycans. The quantitation of glycans on cell surface was achieved by using glycan modified microspheres to simulate the cells, on which the amounts of glycans were detected with enzyme linked immunosorbent assay . The proposed sensing strategy had been used for the detection of mannose and galactose on HeLa cells with Concanavalin A and peanut agglutinin as the specific lectins, which showed 1.37 × 10 8 mannose and 1.13 × 10 8 galactose on each cell, respectively. By treating HeLa cells with exoglycosidases , the mannose and galactose expression levels showed the same changes as those detected with flow cytometric analysis, indicating the practical application of the OECT-based biosensor in cell surface glycan expression analysis.
研究领域:
生物传感器
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分析化学
·
细胞生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Ming Li,Weipeng Teng,Wenying Lu,Mingna Sun,Jinsheng Duan,Xuchun Qiu
The improvement of dual-mode techniques was of particular interest to researchers, which might enhance the detection performance and applicability. Here, a dual-mode optical aptasensor (DO-aptasensor) platform based on exonuclease I (Exo I) cyclic digestion and synergistic enhancement strategy had proposed for zearalenone (ZEN). Following the preparation of dumbbell-shaped signal probe, the Exo I-based cyclic digestion amplification performed, and then the synergistic enhancement effect carried out to achieve the Poly-HRP-based colorimetry and FAM-SGI-based fluorescence. The efficient homogeneous system realized through the magnetic separation, while the signal interference further eliminated by the graphene oxide (GO). The LOD values were as low as 0.067 ng mL −1 for colorimetry mode and 0.009 ng mL −1 for fluorescence mode, which reduced 23-fold and 172-fold than ELASA by same ZEN-Apt. This promising platform gave rise to a dual-mode optical readout, improved sensitivity and positively correlated detection. Meanwhile, the DO-aptasensor also exhibited the acceptable specificity, desirable reliability and excellent practicability. This novel avenue of aptasensor platform hold great potential for dual-mode optical monitoring of other targets, which can further expand the application scope of Exo I-based signal amplification and synergistic enhancement effect.
研究领域:
分析化学
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生物传感器
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食品安全
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2019
·
IF 13.903
Peiru Wang,Weitao Yang,Shuzhan Shen,Chao Wu,Long Wen,Qian Cheng,Bingbo Zhang,Xiuli Wang
Elevated hydrogen peroxide (H2O2) in biological tissues is generally recognized to be relevant to the carcinogenesis process that regulates the proliferative activity of cancer cells and the transformation of malignant features. Inspired by this observation, it can be hypothesized that imaging H2O2 in the tumor microenvironment (TME) could help diagnose tumor types and malignancy, and even guide precise therapy. Thus, in this study, a noninvasive photomedicine strategy is demonstrated that leverages the different levels of H2O2 in the TME, and two representative skin cancers, malignant melanoma (MM, clinically higher incidence of metastasis and recurrence) and cutaneous squamous cell carcinoma (cSCC, relatively less dangerous), are differentially diagnosed. The working probe used here is one we previously developed, namely, intelligent H2O2 responsive ABTS-loaded HRP@Gd nanoprobes (iHRANPs). In this study, iHRANPs have advantages over ratiometric imaging due to their bimodal imaging elements, in which the inherent magnetic resonance imaging (MR) mode can be used as the internal imaging reference and the H2O2 responsive photoacoustic (PA) imaging modality can be used for differential diagnosis. Results showed that after intravenous injection of iHRANPs, the tumor signals on both MM and cSCC are obviously enhanced without significant difference under the MR modality. However, under the PA modality, MM and cSCC can be easily distinguished with obvious variations in signal enhancement. Particularly, guided by PA imaging, photothermal therapy (PTT) can be precisely applied on MM, and a strong antitumor effect was achieved owing to the excessive H2O2 in the TME of MM. Furthermore, exogenous H2O2 was injected into cSCC to remedy H2O2 deficiency in the TME of cSCC, and an evident therapeutic efficacy on cSCC can also be realized. This study demonstrated that MM can be differentially diagnosed from cSCC by noninvasive imaging of H2O2 in the TME with iHRANPs; meanwhile, it further enabled imaging-guided precision PTT ablation, even for those unsatisfactory tumor types (cSCC) through exogenously delivering H2O2.
研究领域:
肿瘤学
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光医学
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生物医学工程
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分子影像
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诊疗一体化
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Qiong-Lin Wang,Hui-Fang Cui,Xiaojie Song,Shuang-Fei Fan,Li-Li Chen,Meng-Meng Li,Zong-Yi Li
Carcinoembryonic antigen (CEA), a glycoprotein, is a wide-spectrum tumor marker for cancer diagnosis. Developing simple, stable, and low-cost methods for CEA detection is of great importance. In this work, a novel, label-free, and antibody-free electrochemical sandwich CEA biosensor was developed based on concanavalin A (ConA) and a DNA aptamer against CEA. Horse radish peroxidase (HRP) was labeled on the sandwich structure for signal production and amplification. Both the CEA and the HRP bind to ConA through sugar-lectin interactions. Under optimum conditions, the detection linear range was from 5 to 40 ng mL −1 CEA, with a detection limit of 3.4 ng mL −1 , lower than the threshold level in human serum for cancer patients (∼10 ng mL −1 ). The biosensor is reproducible, accurate, specific, and stable, with RSD value of 3% for inter-biosensors, and relative error of <13% compared with those obtained by a commercial diagnostic kit. No obvious responses were observed toward interfering proteins. Storage did not cause obvious signal diminishment for the biosensor. The simple and low-cost aptasensor is promisingly applicable for CEA detection in cancer diagnosis, and could be able to serve as a general platform for detection of other interested glycoproteins.
研究领域:
生物传感器
·
电化学
·
癌症诊断
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分析化学
·
生物技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ling-Xin Kong,Yan-Xin Zhang,Wei-Gang Zhang,Yun-Shan Zhang,Tie-Yi Yan,Peng-Cheng Geng,Biao Wang
Lab-on-tip technology opens a new door for optical fiber surface plasmon resonance (SPR) sensors. In this paper, we first demonstrate an ultra-compact reflective SPR microtip probe on the flat fiber end in experiment, which has a length of tens of microns and a diameter of several microns. Simulations and experiments show that this new fiber SPR microtip probe coated with a nano-goldfilm has excellent refractive index (RI) sensing characteristic and great potential for biochemical detection at single cell level. Based on the high RI sensitivity, a bio-probe assembled with Chitosan/Polysodium Styrene Sulfonate (Cs/PSS) nano-polyelectrolyte film and the sheep anti-human IgG-HRP is prepared to recognize human IgG specifically. Due to the high surface sensitivity, the SPR microtip probe exhibits an ultra-low detection limit for human IgG. Due to the high bulk sensitivity and high thermo-optical coefficient (TOC) of polydimethylsiloxane (PDMS), a reference temperature probe based on SPR microtip is proposed, which is used to compensate for the temperature cross-sensitivity of the bio-probe. This new SPR microtip probe can be used not only in optical fiber sensor, but also in Tip-Enhanced Near-Field Raman Microscopy and Scanning Near-Field Optical Microscope.
研究领域:
光纤传感器
·
表面等离子体共振(SPR)技术
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生化传感
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纳米技术
·
显微镜
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）