TALANTA
·
2018
·
IF 4.244
Ke Li Lin,Tong Yang,Hong Yan Zou,Yuan Fang Li,Cheng Zhi Huang
Here we first reported that graphitic carbon nitride nanosheet (g-C 3 N 4 NS) could effectively quench the chemiluminescence (CL) of luminol-hydrogen peroxide (H 2 O 2 ) system. According to the new discovery, a label-free and homogeneous CL aptasensing platform was designed for sensitive detecting of biomarkers. In the absence of target, DNA probe containing hemin/G-quadruplex DNAzyme structure was adsorbed on the surface of g-C 3 N 4 NS, causing the CL quenching of luminol through an electron transfer process. However, in the presence of the target, a DNA-DNA duplex was formed due to DNA hybridization reaction and target recognition effect, which could not be adsorbed onto the g-C 3 N 4 NS surface because of its weak affinity. Thus, the electron transfer was blocked and the CL emission of luminol could be enhanced. The proposed CL aptasensor could detect carcinoembryonic antigen (CEA) with a detection limit of 63.0 pg/mL and it can also be used as a general detecting strategy for adenosinetriphosphate (ATP) detection. This aptasensing platform exhibited high sensitivity toward biomarkers and the probe need not be labeled, showing great promise for disease diagnosis.
研究领域:
生物化学
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分析化学
·
医学诊断
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DOI
PubMed
高影响因子
ChemPlusChem
·
2018
·Vol. 83
·期 11
·1038-1043
·
IF 3.205
Song Wang,Hejin Jiang,Li Zhang,Jian Jiang,Minghua Liu
Graphical Supramolecular gels formed from hemin and histidine amphiphiles were tested as artificial enzymes to catalyse the oxidation of the chiral substrate L-DOPA to L-dopachrome. The supramolecular gel made up of left-handed helices enhanced the catalytic activity of hemin effectively, and more interestingly, it exhibited enantioselectivity. Supramolecular co-assembly provides an efficient way to combine structural and functional units through noncovalent interactions and produce materials with enhanced performance. Here, using a chiral amphiphilic histidine derivative (LHC18 or DHC18) as a gelator, we have successfully co-assembled the gelator and hemin through gelation. During the co-assembly, the chiral information of the gelator was transcribed into the assemblies, and thus right- and left-handed helical ribbons from LHC18-Hemin and DHC18-Hemin co-assemblies were obtained, respectively, which were confirmed by CD spectra and SEM observations. These helical ribbons were used as artificial enzymes to catalyse the oxidation reaction of 3,4-dihydroxy-L-phenylalanine (L-DOPA). The catalytic activity of hemin in supramolecular gels can be effectively enhanced over that of free hemin. Moreover, by virtue of induced chirality, the supramolecular gels of LHC18-Hemin and DHC18-Hemin exhibited obvious enantioselective activity. This work provides a way to combine chirality transfer and supramolecular asymmetric catalysis.
研究领域:
生物化学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2013
·
IF 3.925
Yuyuan Yao,Yajun Mao,Qunfeng Huang,Lie Wang,Zhengfu Huang,Wangyang Lu,Wenxing Chen
In this study, we evaluated a novel heterogeneous catalytic system (hemin-ACF/H 2 O 2 ) based on activated carbon fiber supported hemin for the rapid removal of dyes. Contrast experiments, effects of pH and temperature, and sustained catalytic stability of hemin-ACF for the catalytic decomposition of azo dye RR195 used as a model pollutant were investigated. Surprisingly, the introduction of ACF significantly enhanced the decomposition of dyes by hemin with an obvious improvement in pH tolerance and stability. Inhibition and probe studies combined with electron paramagnetic resonance (EPR) were conducted to ascertain the role of several radicals ( OH, O 2 − /HO 2 , 1 O 2 and Fe IV O) on dye degradation. ACF are rich in free electrons, and the π-conjugated macrocyclic structure of hemin may present convenient channels for the transfer of free electrons from the ACF, promoting the generation of hydroxyl radicals and high-valence iron species. These results are promising because they offer new insight for the application of hemin as a catalyzer upon treatment of organic pollutants and contribute to identification of the interaction between support material and catalyzer from a new perspective.
研究领域:
环境科学
·
催化化学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2013
·
IF 3.925
Ruiling Sun,Yong Wang,Yongnian Ni,Serge Kokot
Phenols are well known noxious compounds, which are often found in various water sources. A novel analytical method has been researched and developed based on the properties of hemin–graphene hybrid nanosheets (H–GNs). These nanosheets were synthesized using a wet-chemical method, and they have peroxidase-like activity. Also, in the presence of H 2 O 2 , the nanosheets are efficient catalysts for the oxidation of the substrate, 4-aminoantipine (4-AP), and the phenols. The products of such an oxidation reaction are the colored quinone-imines (benzodiazepines). Importantly, these products enabled the differentiation of the three common phenols – pyrocatechol, resorcin and hydroquinone, with the use of a novel, spectroscopic method, which was developed for the simultaneous determination of the above three analytes. This spectroscopic method produced linear calibrations for the pyrocatechol (0.4–4.0 mg L −1 ), resorcin (0.2–2.0 mg L −1 ) and hydroquinone (0.8–8.0 mg L −1 ) analytes. In addition, kinetic and spectral data, obtained from the formation of the colored benzodiazepines, were used to establish multi-variate calibrations for the prediction of the three phenol analytes found in various kinds of water; partial least squares (PLS), principal component regression (PCR) and artificial neural network (ANN) models were used and the PLS model performed best.
研究领域:
环境科学
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分析化学
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纳米材料科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 8
·1-9
·
IF 6.408
Liu Gengjun,Guan Xiaohan,Li Binxiao,Zhou Hong,Kong Na,Wang Haiyan
An ultrasensitive luminol electrochemiluminescence (ECL) immunosensor was constructed for the detection of prostate specific antigen (PSA) using glucose oxidase–decorated hemin-graphene oxide-gold nanoflowers ternary nanocomposites as probes. Graphene oxide was first modified with hemin and then with gold nanoflowers through an in situ growth method, which has significantly boosted the catalytic activity of this graphene oxide–based peroxidase mimetics. The biocatalytical activity of this ECL immunosensor was thoroughly investigated to achieve selective recognition of the analyte molecules (PSA) by specific binding between antigens and antibodies. The limit of detection was calculated to be 0.32 pg mL −1 with a signal-to-noise ratio of 3. A broad linear range from 7.5 × 10 −4 to 2.5 ng mL −1 was obtained. Such step-by-step assembled biosensor showed controlled nanostructure and exhibited promising application in analysis of human serum samples with a recovery range of 90.6–111.8% and a RSD range of 3.9–5.5%. Graphical abstract
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 31
·13231-13240
·
IF 7.79
Qing Wang,Naijie Wei,Jingru Guo,Kai Feng,Yin Kwan Wong,Jingwei Zhang,Jigang Wang,Xiaolian Sun
Although artemisinin (ART) has shown initial promise in cancer therapy, its therapeutic efficacy is limited by its low tumor inhibitory efficacy and unfavorable distribution. Considering the important role of heme in the specific parasite-killing effect of ART, we designed a liposomal nanostructure self-assembled from hemin-lipid (Hemesome) to co-deliver ART and hemin for cancer therapy. The synergistic chemotherapeutic and immunotherapeutic effects of hemin and ART were demonstrated both in vitro and in vivo. The liposome-like structure was relatively stable in the blood circulation and gastrointestinal tract environment, but dissociated in the tumor cell environment. The folic acid (FA) modification not only increased their efficiency for transport across the epithelium, but also increased their tumor accumulation. In mouse models, following oral administration of FA-Hemesome-ART nanoparticles (5 mg kg−1 ART in total) every other day and intraperitoneal injection with a programmed death-ligand 1 antibody (aPD-L1, 70 μg per mouse in total), MC38 tumors were completely inhibited within 30 days. The cured mice remained tumor-free 30 days after rechallenging them with another inoculation of MC38 cells due to the strong immune memory effect.
研究领域:
药学
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肿瘤学
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纳米技术
·
免疫学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Kunming Sun,Tinghui Deng,Jing Sun,Shuo Gao,Huwei Liu,Shuyun Zhu,Xian-En Zhao
Artemisinin (ART) is a type of frontline drug to treat drug-resistant falciparum malaria. Simple, accurate and selective determination of ART is significant to monitor its clinical pharmaceutical efficacy. Herein, a new ratiometric fluorescence method has been designed for the determination of ART with Zn-MOF as fluorescence reference and hemin as catalyst, respectively. Zn-MOF possesses intrinsic fluorescence at 443 nm owing to 2-aminoterephthalic acid ligand. When o -phenylenediamine (OPD) is mixed with hemin, a weak fluorescent signal at 570 nm ascribed to oxidized product of OPD (oxOPD) is observed. In the presence of ART, hemin can catalyze ART to break its peroxide bridge and release a large number of reactive oxygen species, which effectively oxidize OPD into luminescent oxOPD. Therefore, the fluorescence at 570 nm is enhanced significantly while the fluorescence of Zn-MOF remains basically unchanged. Thus, a ratiometric fluorescence sensing platform has been constructed for the detection of ART. This method exhibits wider linear range (0.15 μM-150 μM) with detection limit of 50 nM. This novel and selective method has been used to detect ART in compound naphthoquinone phosphate tablets.
研究领域:
药学
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分析化学
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生物医学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Shiguan Xu,Lijun Yan,Hui Cheng,Hui Xie,Lina Zeng,Lin Li,Shuhai He,Wei Sun
In this paper an electrochemical sensor was fabricated by dropping a graphdiyne (GDY) and hemin suspension solution on the surface of carbon ionic liquid electrode (CILE) followed by casting the Nafion film (Nafion/hemin-GDY/CILE). The morphology of GDY was observed by scanning electron microscopy with the biomolecular structure of hemin-GDY and hemin characterized by UV-Vis absorption spectrophotometry, which proved that hemin maintained the original structure after mixing with GDY. Direct electrochemistry of hemin on the GDY modified electrode was realized with a pair of well-defined redox peaks and electrochemical performances were checked in detail with the electrochemical parameters calculated. Nafion/hemin-GDY/CILE showed excellent electrocatalytic ability towards the reduction of bromate with the detection range from 5.00 to 50.0 mmol L -1 and the detection limit of 3.3 mmol L -1 (3 σ ). In addition, the modified electrode possessed good reproducibility and stability, which was applied to the analysis of seawater samples.
研究领域:
分析化学
·
电化学传感器
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 8
·087517
·
IF 4.386
Shengnan Li,Xinhao Li,Liping Cao,Chaoxian Wang,Jintao Liang,Zhide Zhou,Guiyin Li
Glypican-3 (GPC3) is a serum marker for hepatocellular carcinoma (HCC) and has excellent specificity and sensitivity. Herein, the construction of an electrochemical aptasensor based on reduced graphene oxide-carboxymethyl chitosan-hemin-palladium nanoparticles (RGO-CMCS-Hemin/Pd NPs) and GPC3 aptamer (GPC3apt) has the function of GPC3 identification and analysis. RGO-CMCS-Hemin/Pd NPs has excellent biocompatibility, large specific surface area and good electrical conductivity, which is not only used as advanced sensing materials to modify the electrode surface to reduce the resistance and increase the signal amplitude, but also used as an electroactive substance to provide electrical signals. GPC3apt as a biometric element makes the aptasensor has good specificity and sensitivity. The GPC3apt can combine with GPC3 and form GPC3-aptamer conjugation, which increases the electron transfer impedance and changes the electrical signal. Under the optimum conditions, the aptasensor had a good linear relationship with the GPC3 concentration in the range of 1.0–100.0 ng ml−1 with R2 of 0.9573. The lower limit of detection was 1.0 ng ml−1. When the aptasensor was applied to GPC3 detection in human serum samples, the recovery was 93.73%–118.83%. In addition, the GPC3 aptasensor has excellent specificity, reproducibility and stability, which is anticipated to be applied for the clinical detection of GPC3.
研究领域:
生物医学工程
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分析化学
·
纳米技术
·
癌症诊断
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 20
·6779
·
IF 4.927
Zhaoshen Li,Xiaochun Deng,Xiaoping Hong,Shengfa Zhao
Compared with natural enzymes, nanozymes have the advantages of good catalytic performance, high stability, low cost, and can be used under extreme conditions. Preparation of highly active nanozymes through simple methods and their application in bioanalysis is highly desirable. In this work, a nanozyme based on dispersion of hemin by graphene quantum dot (GQD) is demonstrated, which enables colorimetric detection of glutathione (GSH). GQD was prepared by a one-step hydrothermal synthesis method. Hemin, the catalytic center of heme protein but with low solubility and easy aggregation that limits its catalytic activity, can be dispersed with GQD by simple sonication. The as-prepared Hemin/GQD nanocomplex had excellent peroxidase-like activity and can be applied as a nanozyme. In comparison with natural horseradish peroxidase (HRP), Hemin/GQD nanozyme exhibited a clearly reduced Michaelis–Menten constant (Km) when tetramethylbenzidine (TMB) was used as the substrate. With H2O2being the substrate, Hemin/GQD nanozyme exhibited a higher maximum reaction rate (Vmax) than HRP. The mechanisms underlying the nanozyme activity were investigated through a free radical trapping experiment. A colorimetric platform capable of sensitive detection of GSH was developed as the proof-of-concept demonstration. The linear detection range was from 1 μM to 50 μM with a low limit of detection of 200 nM (S/N = 3). Determination of GSH in serum samples was also achieved.Keywords:nanozyme;graphene quantum dot;hemin;peroxidase-like activity;colorimetric determination of glutathione
研究领域:
生物化学
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纳米技术
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Small
·
2023
·
IF 13.3
Ke Cheng,Henggang Wang,Shan Sun,Mingyu Wu,Hongzhe Shen,Ke Chen,Zhiyuan Zhang,Si Li,Hengwei Lin
Antibacterial photodynamic therapy (aPDT) is a promising antibiotics-alternative strategy for bacterial infectious diseases, which features broad-spectrum antibacterial activity with a low risk of inducing bacterial resistance. However, clinical applications of aPDT are still hindered by the hydrophobicity-caused inadequate photodynamic activity of conventional photosensitizers and the hypoxic microenvironment of bacterial infections. To address these problems, herein, a promising strategy is developed to achieve specific chemiluminescence (CL) imaging and enhanced PDT of bacterial infections using hemin-modified carbon dots (H-CDs). The H-CDs can be facilely prepared and exhibit favorable water solubility, augmented photodynamic activity, and unique peroxidase-mimicking capacity. Compared with the free CDs, the photodynamic efficacy of H-CDs is significantly augmented due to the increased electron–hole separation efficiency. Moreover, the peroxidase catalytic performance of H-CDs enables not only infection identification via bacterial infection microenvironment-responsive CL imaging but also oxygen self-supplied aPDT with hypoxia-relief-enhanced bacteria inactivation effects. Finally, the enhanced aPDT efficiencies of H-CDs are validated in both in vivo abscess and infected wound models. This work may provide an effective antibacterial platform for the selective imaging-guided treatment of bacterial infections.
研究领域:
生物医学工程
·
纳米技术
·
药学
·
传染病
·
治疗诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Xiaojuan Yi,Yonghua Yuan,Min Qing,Lulu Wang,Huizhen Li,Lijuan Bai
Glucose management is an important part of disease control for diabetes patients, thus the development of a rapid and real-time point of care testing (POCT) device for monitoring blood glucose is of great significance. In this work, a paper-based analytical device (PAD) is constructed by combining acetylene black (AB)-hemin complex modified filter paper as sensing platform with a smartphone as signal detector. Large specific surface area of AB decreases the self-associate and aggregate of hemin in aqueous solution, resulting in improved peroxidase-like activity of hemin. Compared with graphene oxide supported hemin, AB-hemin exhibits superior signal response on paper. Glucose oxidase (GOx) catalyzes the conversion of blood glucose to hydrogen peroxide, and then AB-hemin complex catalyzes the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue TMB oxidized products (TMB + ) in the presence of hydrogen peroxide, thus achieving the visual detection of blood glucose. In optimal conditions, PAD provides an applicable linear range from 0.2 mM to 30 mM and a low limit of detection (LOD) (0.06 mM). Notably, the detection accuracy of the developed paper-based sensor is in good agreement with that of the commercially available blood glucose meter (p > 0.05). Moreover, the proposed PAD presents high recoveries from 95.4% to 112% (RSD ≤ 3.2%), and therefore holds great potential for glucose monitoring and diabetes diagnosis.
研究领域:
生物医学工程
·
分析化学
·
即时检测
·
糖尿病管理
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）