MICROCHIMICA ACTA
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2017
·Vol. 184
·期 9
·3301-3308
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IF 4.58
Zhou Yunlei,Wang Minghui,Yin Huanshun,Ai Shiyun
The authors describe the fabrication of an electrochemical immunosensor for the determination of the activity of protein kinase A (PKA). The method involves (a) electrochemical deposition of gold nanoparticles (AuNPs) on a glassy carbon electrode, (b) PKA-induced catalytic phosphorylation of serine, and (c) the use of phosphoserine antibody and horseradish peroxidase conjugated to IgG on gold nanoparticles (HRP-IgG-AuNPs). The use of AuNPs and HRP-IgG-AuNPs results in large amplification so that the method, at a typical working potential as low as 0.08 V (vs. SCE), has a linear range that extends from 0.1 to 50 activity units per mL, and the detection limit is 0.026 units per mL (at an S/N ratio of 3). The assay is selective (not the least due to a rather low working potential) and well reproducible. It may also be applied to screening for PKA inhibitors and to quantify the PKA activity in human cell lysates.
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研究领域:
分析化学
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生物化学
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分子生物学
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电化学
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纳米技术
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DOI
高影响因子
OPTICS AND LASERS IN ENGINEERING
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2020
·
IF 4.273
Hang Song,Qi Wang,Wan-Ming Zhao
In this work, we proposed a novel surface plasmon resonance (SPR) sensitivity-enhancing method by adding MoS 2 nanosheets between gold film and fiber (fiber-MoS 2 -gold film). And we apply it to the detection of human IgG protein. The sensor was fabricated by attaching MoS 2 nanosheets to the U-shaped fiber surface and then covering a layer of gold film. The refractive index sensitivity of 6184.4 nm/RIU was achieved by the fiber-MoS 2 -gold film sensor which is 59 percent higher than the gold film SPR sensor and 25 percent than the fiber-gold film-MoS 2 SPR sensor.The presence of MoS 2 increases the absorption of light energy and excites a stronger SPR phenomenon. At the same time, since the monolayer MoS 2 is a direct bandgap semiconductor, the absorbed light energy promotes the transfer of electrons, generates strong coupling on the gold film surface, enhances the surface electric field, and improves the sensitivity of the sensor. Biofunctionalized sensor detect human IgG with the limit of detection as low as 19.7 ng/mL. This work proposed a novel method for enhancing the sensitivity of SPR sensor, it owns a higher sensitivity and a lower LOD which has broad application prospects in measuring low concentration biological analytes.
研究领域:
光学
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生物传感
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纳米技术
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分析化学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2019
·Vol. 43
·期 36
·14220-14223
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IF 3.069
Kai-Xin Xie,Shu-Shu Jia,Jin-Hua Zhang,Huan Wang,Qiang Wang
The enhancement of surface plasmon-coupled emission (SPCE) by CNT assistance was investigated with the factor of 12 and 65 as compared to normal SPCE and free space emission, respectively. This strategy designed as an immunosensor to detect IgG with a detection limit of 0.0025 ng mL−1 provides a universal way to improve the detection sensitivity in fluorescence-based sensing platforms.
研究领域:
纳米技术
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光学
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分析化学
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生物传感器
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DOI
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Xu-mei Wang,Zong-cai Tu,Yun-hua Ye,Guang-xian Liu,Yue-ming Hu,Hui Wang
This study aimed to isolate and evaluate the allergenicity of glycated α-lactalbumin (ALA) digestive products and identify its allergenic peptides. The digestive products of native-, alone glycated- and ultrasound-assisted glycated ALA (ALA-D, ALA-gal-D, 100ALA-gal-D) were isolated into three fractions (F1, F2 and F3). High-resolution mass spectrometry showed that the digestion-resistant peptides of F2 and F3 mainly distributed in amino acid sequence (AA) 25–31, AA32-53, AA40-53, AA54-60, AA80-90, AA94-104. The allergenicity of the three fractions of glycated ALA was lower than that in ALA-D, indicating glycation of ALA could indeed reduce its allergenicity after digestion. Furthermore, most fractions isolated from high glycation-degree ALA had the lowest allergenicity. The IgG/IgE binding abilities of synthesized peptides indicated that AA94-104 firstly identified by us embodied the strongest allergenicity and might be the potential allergenic peptide. This will provide a theory for preparing hypoallergenic products based on the identified allergenic peptides.
研究领域:
食品科学
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过敏学
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蛋白质组学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Pan Cheng,Mingyue Li,Tiange Chen,Yuxi Wang,Qihao Guo,Qin Cheng,Tao Mei,Yang Xiang,Ke Liu,Dong Wang
Constructing a nanofibrous membrane with high flow rate surface pore structure and high-density ligand chemical structure is a promising strategy to balance the trade-off between high flow rates and high adsorption capacity for protein separation and purification. Herein, a nanofiber-based ion-exchange chromatography membrane with a periodic diagonal surface structure and high ionic strength ligands was fabricated using dispersion in situ cross-linking, wet coating, and template printing with a three-wire diagonal woven mesh. For this membrane, EVOH nanofibers were used as skeleton, glutaraldehyde (GA) as cross-linking agent, and quaternized chitosan (QCS) as binder and functional ligand. The results show that when QCS content was 35%, the morphology, pore size, and comprehensive performance of NFM-QCS were optimized. NFM-QCS demonstrated high flow rates under low pressure, a high static adsorption capacity of 1285.16 mg/g, and a high dynamic adsorption capacity of 135.63 mg/mL (1 mm/min) for bovine serum albumin (BSA), which is superior to reported state-of-the-art and commercial ion-exchange chromatography columns. Based on the excellent adsorption performance, NFM-QCS can efficiently separate and purify IgG from the mixed solution of BSA and IgG. Our work provides a facile approach for the preparation of a high-performance ion-exchange chromatography membrane for protein separation.
研究领域:
生物工程
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材料科学
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化学工程
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2017
·Vol. 410
·期 6
·1817-1824
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IF 3.431
Jiang Jia,Tian Sizhu,Wang Kun,Wang Yang,Zang Shuang,Yu Aimin,Zhang Ziwei
With the help of iron oxide nanoparticles, electron spin resonance spectroscopy (ESR) was applied to immunoassay. Iron oxide nanoparticles were used as the ESR probe in order to achieve an amplification of the signal resulting from the large amount of Fe 3+ ion enclosed in each nanoparticle. Rabbit IgG was used as antigen to test this method. Polyclonal antibody of rabbit IgG was used as antibody to detect the antigen. Iron oxide nanoparticle with a diameter of either 10 or 30 nm was labeled to the antibody, and Fe 3+ in the nanoparticle was probed for ESR signal. The sepharose beads were used as solid phase to which rabbit IgG was conjugated. The nanoparticle-labeled antibody was first added in the sample containing antigen, and the antigen-conjugated sepharose beads were then added into the sample. The nanoparticle-labeled antibody bound to the antigen on sepharose beads was separated from the sample by centrifugation and measured. We found that the detection ranges of the antigen obtained with nanoparticles of different sizes were different because the amount of antibody on nanoparticles of 10 nm was about one order of magnitude higher than that on nanoparticles of 30 nm. When 10 nm nanoparticle was used as probe, the upper limit of detection was 40.00 μg mL −1 , and the analytical sensitivity was 1.81 μg mL −1 . When 30 nm nanoparticle was used, the upper limit of detection was 3.00 μg mL −1 , and the sensitivity was 0.014 and 0.13 μg mL −1 depending on the ratio of nanoparticle to antibody.
Schematic diagram of procedure and ESR spectra
研究领域:
分析化学
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纳米技术
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2018
·
IF 8.097
Dan-Dan Zhang,Zhi-Yong Guo,Peng-Fei Guo,Xue Hu,Xu-Wei Chen,Jian-Hua Wang
Polyoxometalate [{a-PW11O39Zr(μ-OH)(H2O)}2]8– (POM1) is first prepared by sandwiching ZrIV among 2 mono-lacunary α-Keggin polyoxometalates, and then novel magnetic nanoparticles (NPs), Fe3O4@polyethyleneimine (PEI)@POM1, are fabricated by coating POM1 onto the surface of magnetic Fe3O4@PEI NPs under electrostatic interaction. The obtained Fe3O4@PEI@POM1 NPs are characterized by Fourier transform infrared, zeta potential, vibrating sample magnetometer, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction. Ascribed to the hydrogen-bonding and electrostatic interactions, the NPs exhibit high adsorption selectivity toward IgG, and the adsorption capacity is high up to 304 mg g–1 under optimal adsorption conditions. By using 0.01% cetyl trimethylammonium bromide to strip the adsorbed protein species, an elution efficiency of 95% is achieved. The feasibility of Fe3O4@PEI@POM1 NPs in real-world sample assay has been demonstrated by the selective isolation of IgG heavy chain and light chain from human serum, as confirmed by the sodium dodecyl sulfate polyacrylamide gel electrophoresis assay.
研究领域:
纳米技术
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材料科学
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2022
·
IF 6.556
Lizhong Zhang,Qiang Huang,Xiaonan Zhang,Zhen Zeng,Hailong Zhang,Tian Guan,Yang Xu,Chongqi Zhou,Lingqin Meng,Gengyu Liang,Zhangyan Li,Bei Wang,Le Liu,Cuixia Guo,Yonghong He
In this study, an interfacial biosensing scheme with ultra-precision is proposed. The scheme uses weak measurement techniques to ensure ultra-high sensitivity of the sensing system while improving the stability of the system through self-referencing and pixel point averaging, thus achieving ultra-high detection accuracy of biological samples. In specific experiments, we have used the biosensor in this study to perform specific binding reaction experiments for protein A and Mouse IgG with a detection line of 2.71 ng/mL for IgG. In addition, the sensor is non-coated, simple in structure, easy to operate, and low in cost of use.
研究领域:
生物传感器
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生物技术
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Cancers
·
2022
·Vol. 14
·期 5
·1333
·
IF 6.575
Jichen Sha,Rongrong Zhang,Jiteng Fan,Yong Gu,Yiqing Pan,Jing Han,Xiaoyan Xu,Shifang Ren,Jianxin Gu
Simple SummaryAs serum IgG glycosylation is associated with various cancers, our goal is to explore whether serum IgG galactosylation and its associated glycans could be used as tumor markers associated with hepatocellular carcinoma (HCC). At the same time, we explore the effect of the B-cell-specific ablation of B4GALT1 on HCC and finally analyze whether the low incidence of female cancer was related to the findings from the above perspective. The results demonstrate that the tumor marker of serum IgG glycosylation is galactosylation and its associated glycans and that the B-cell-specific ablation of B4GALT1 reduces HCC formation by reducing serum IgG galactosylation levels and by modulating the associated glycans, meaning that the lower incidence of cancer in women may be related to minor changes in the B-cell B4GALT1 and unchanged serum IgG galactosylation levels. This study aims to provide a theoretical basis for the early diagnosis and prevention of HCC and to determine why it has such a high incidence in males.AbstractSerum immunoglobulin G (IgG) glycosylation, especially galactosylation, has been found to be related to a variety of tumors, including hepatocellular carcinoma (HCC). However, whether IgG glycan changes occur in the early stages of HCC formation remains unclear. We found that the galactosylation level increased and that the related individual glycans showed regular changes over the course of HCC induction. Then, the effect of the B-cell-specific ablation of β1,4galactosyltransferase 1 (CKO B4GALT1) and B4GALT1 defects on the IgG glycans that were modified during the model induction process and HCC formation is investigated in this study. CKO B4GALT1 reduces serum IgG galactosylation levels and reduces cancer formation. Furthermore, insignificant changes in the B-cell B4GALT1 and unchanged serum IgG galactosylation levels were found during cancer induction in female mice, which might contribute to the lower cancer incidence in female mice than in male mice. The gender differences observed during glycan and B4GALT1 modification also add more evidence that the B4GALT1 in B cells and in serum IgG galactosylation may play an important role in HCC. Therefore, the findings of the present research can be used to determine the methods for the early detection of HCC as well as for prevention.Keywords:hepatocellular carcinoma;immunoglobulin G;glycan;β1,4-galactosyltransferase 1;mouse
研究领域:
医学
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分子生物学
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DOI
PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2026
·Vol. 51
·期 1
·73
·
IF 4.5
Zhou Juan,Li Haipeng,Wen Ying,He Lingli,He Yangli,Meng Heng
Neuromyelitis optica spectrum disorder (NMOSD) is a rare but serious inflammatory demyelinating disease. A key characteristic of NMOSD is the presence of a pathogenic autoantibody in serum called aquaporin-4 immunoglobulin G (AQP4-IgG). This study investigates the mechanism of astrocyte-derived small extracellular vesicles (EVs) carrying mitochondrial DNA (mtDNA) to promote AQP4-IgG-induced microglial activation in neuromyelitis optica (NMO) via the toll-like receptor 9 (TLR9)/myeloid differentiation primary response 88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway. Serum IgG was isolated from NMOSD patients (AQP4-IgG) and healthy controls (Con-IgG). Astrocytes were treated with AQP4-IgG or Con-IgG. EVs were isolated via ultracentrifugation, characterized, and examined for internalization. Microglia were exposed to EVs, and mtDNA levels were assessed. An NMO mouse model was established, with neurological damage, mouse behaviors, tissue damage, and microglial characterization evaluated using modified neurological severity score, open-field test, rotarod test, luxol fast blue staining, and flow cytometry. Inflammatory cytokines, TLR9, MyD88, p65, IκBα, p-p65, and p-IκBα in BV2 cells and spinal cord tissues were analyzed via ELISA, RT-qPCR, and western blot. AQP4-IgG-induced astrocyte-derived EVs increased Iba1-high-expressing and CD86/tumor necrosis factor-α-high-expressing cells, reduced CD206/transforming growth factor-β-high-expressing cells, and boosted inflammatory responses. AQP4-IgG-induced EVs carried mtDNA to activate microglia via the TLR9/MyD88/NF-κB pathway. TLR9/MyD88/NF-κB pathway inhibition reversed AQP4-IgG-induced EVs’ promotion on microglial activation. In vivo, AQP4-IgG-induced EVs-mtDNA exacerbated microglial activation and NMO through the TLR9/MyD88/NF-κB pathway. AQP4-IgG-induced EVs carried mtDNA to upregulate TLR9, further activating the MyD88/NF-κB pathway, thereby promoting microglial activation and transition toward pro-inflammatory gene-high-expressing cells to drive NMO progression.
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PubMed
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 22
·4052-4059
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IF 4.543
Heping Wang,Fenglong Jiao,Fangyuan Gao,Junjie Huang,Yan Zhao,Yehua Shen,Yangjun Zhang,Xiaohong Qian
Protein glycosylations play important roles in various biological processes and in the disease progression of organisms. The development of specific enrichment materials and strategies before mass spectrometric analysis was a prerequisite to glycoproteomic analysis due to the difficulty caused by substoichiometric levels of glycoproteins. In this work, novel magnetic covalent organic frameworks (denoted as Fe3O4@TpPa-1) were first developed using only a two-step solvothermal reaction and then applied in the hydrophilic enrichment of glycopeptides. Sea urchin-type composites with super-paramagnetic properties were constructed by in situ growth of TpPa-1 covalent organic frameworks on the surface of magnetic nanoparticles. A total of 37 and 22 glycopeptides could be easily detected from IgG and HRP digests, respectively, by hydrophilic enrichment with the newly developed materials. An ultralow detection limit (28 fmol), satisfactory selectivity and high recovery could be achieved using Fe3O4@TpPa-1. The material's excellent enrichment performance was also demonstrated using glycopeptide analysis in real complex samples. Within three independent replicates, 228 glycopeptides corresponding to 114 glycoproteins could be detected from human serum digests, which is better than the performance obtained by commercial HILIC materials. The results suggest that covalent organic frameworks show potential for application in glycoproteomic studies.
研究领域:
分析化学
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生物化学
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材料科学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 20
·6861-6868
·
IF 7.059
Peili Zhao,Yaofei Chen,Yu Chen,Shiqi Hu,Hui Chen,Wei Xiao,Guishi Liu,Yong Tang,Jifu Shi,Zhendan He,Yunhan Luo,Zhe Chen
Biosensors based on surface plasmon resonance (SPR) have shown significant applications in drug screening, environmental monitoring, immunoassay, etc.; however, improving the sensitivity of these biosensors is one of the prime tasks at present. In this study, taking advantage of the special optical and morphological characteristics of MoS2 nanoflowers, we synthesized a sensitivity-improved SPR biosensor by successively depositing MoS2 nanoflowers and gold nanoparticles (AuNPs) on a traditional SPR sensor and then applied this biosensor to the immunoassay of mouse IgG. It was found that the deposition of the MoS2 nanoflowers could improve the bulk refractive index sensitivity from 2212.2 to 2857.2 nm per RIU (RIU: refractive index unit); however, the further addition of AuNPs resulted in a decrease in the bulk refractive index sensitivity to 2149.7 nm per RIU. Moreover, when the proposed biosensor was applied to the IgG immunoassay, a 0.047 nm (μg mL−1)−1 sensitivity of the MoS2–AuNP-modified sensor was achieved, which was ∼3 times that of the unmodified sensor (0.016 nm (μg mL−1)−1). This improvement in the sensitivity of the biosensor towards IgG can be attributed to the flower-like morphology of MoS2 that provides more sites for the loading of AuNPs, thus facilitating the immobilization of a larger amount of IgG antibody on the surface of the sensor. Because of its simple fabrication process and compatibility with the standard bio-activation method of gold surface, the proposed biosensor provides an easily-accessible platform for biological analysis and detection with improved sensitivity.
研究领域:
生物技术
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纳米技术
·
分析化学
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生物传感
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产品关联
DOI
高影响因子
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