SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Qiaoqiao Ci,Hong Yang,Yuanzhi Bian,Zheng Li,Jinhua Liu,JiFu Wei,Naidi Yang,Juhong Chen,Lin Li,Chengwu Zhang
Currently, the development of simple, fast, low-cost, and environmentally friendly MAO-A specific detection methods remains a huge challenge. Herein, a simple in situ fluorescent copolymer dots (FCDs)-based kit is constructed for the specific detection of monoamine oxidase-A in cell, tissue and human prostate cancer. The developed kit is depended on the high reaction activity between epinephrine and polyethyleneimine to form FCDs and the selective catalyzing activity of MAO-A on epinephrine. The gained FCDs are identified and characterized by high-resolution TEM, absorption and fluorescence spectrum. The present kit achieves highly sensitive detection of MAO-A with a limit of detection (LOD) of 20 ng/mL as well as screening of inhibitor with an IC50 (the concentration responding to 50% inhibition) of 1.071 μM by using clorgyline as a model. The MAO-A and MAO-B as the two isoforms of MAO are discriminated successfully, demonstrating the excellent selectivity of our kit. Noteworthy, the proposed method is applicable for measuring MAO-A not only from serum samples, but also for endogenous MAO-A in cells, mouse tissues and human prostate tumors with satisfied results. The proposed kit will be a great potential tool for human prostate cancer (PCa) diagnosis and the development of anti-PCa drug screening.
研究领域:
癌症生物学
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分析化学
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生物医学工程
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药理学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Chucheng Wang,Junshan Han,Danni Xue,Chenjie Gu,Shuwen Zeng,Junhui Jiang,Tao Jiang,Xing Li,Kerong Wu
Surface-enhanced Raman scattering (SERS) technique with high sensitivity, reliable specificity, and rapid recognition ability exhibits attractive promise for the effective fast-monitoring of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein, a novel SERS-active immunoassay kit for SARS-CoV-2 nucleocapsid (N) protein was prepared by in-situ growing gold (Au) nanowire forests (NFs) onto Ti 3 C 2 T x , which was then modified onto polymethyl methacrylate (PMMA) matrix and encapsulated into kit. It was noted that the Au nanowires with fibrous structures which vertically anchored on Ti 3 C 2 T x served as perfect channels to promote photo-induced charge transfer. The synergistic action of electromagnetic and chemical effects resulted in an enhancement factor (EF) of 1.27 × 10 7 . Furthermore, the unreliable fluctuation of the enhanced signal was eliminated by using the intrinsic Raman signal of the flexible PMMA platform, achieving an improved correlation coefficient (R 2 ) value from 0.950 to 0.990. Moreover, the as-designed immunoassay kit with both high sensitivity and remedied quantitative ability rendered by the Ti 3 C 2 T x @Au NFs-PMMA composite exhibited a powerful performance in the practical detection of N-protein with concentration low to 5.0 × 10 −8 mg/mL.
研究领域:
分析化学
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材料科学
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生物医学工程
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病毒学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-12
·
IF 5.3
Meng Xiao,Liu Lingfei,Wang Haitong,Zhang Mengping,Zou Wei,Wu Hongmin,Chen Wenwen,Li Nianlu
A portable kit was developed for on-site high-throughput detection of methyl parathion (MPT) in vegetables by integrating solid-phase microextraction and SERS technology. This portable kit consists of a 96-well plate coated with a copper foil modified by silver nanocrystals, which was fabricated by a galvanic displacement method. The 96-well plate allows multiple samples to be pretreated simultaneously, with each well featuring small pits that isolate individual samples, effectively preventing cross-contamination. Furthermore, the SERS substrate allows the enrichment of MPT on its surface, enhancing detection sensitivity and specificity. The method detection limits calculated from the characteristic peak of MPT is 0.27 ng/cm 2 ( S / N = 3). MPT on spinach, green pepper, and tomato surfaces were directly determined rapidly and the sensitivities were 1.42 ng/cm 2 , 0.84 ng/cm 2 , and 0.87 ng/cm 2 , respectively. The versatility and reliability of this approach underscore its potential for routine pesticide residue screening. Looking ahead, further optimization of this platform could extend its applicability to a broader spectrum of organophosphorus pesticides, bolstering food safety monitoring and reducing potential health risks.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Can Chen,Shiyu Zhang,Chengwu Zhang,Lin Li,Jixin Zhu,Jinhua Liu
Detection of H 2 O 2 is significant for the applications in environmental protection, pharmaceutical industries , food production, and clinical control. Current colorimetric kit of H 2 O 2 based on enzyme or nanomaterials always needs TMB or other peroxidases substrate as coloration species. Herein, we report a simple hydrothermal method to synthesize of TMB dot as the nanosubstrate construction colorimetric kit for highly selective and sensitive detection of H 2 O 2 based on Fenton reaction . Compared with TMB as the substrate, the synthesized TMB dots (TMBDs) show higher reaction activity in the presence of the same low-level Fe 2+ and H 2 O 2 , resulting in highly sensitive sensing H 2 O 2 down to 0.8 nM. Additionally, the obtained TMBDs can well disperse in solution, while generally TMB as substrate needs to be dissolved in organic solvents such as DMF and DMSO. Furthermore, the developed colorimetric kit is applied in detection of Mao-A, due to the reaction between epinephrine and Mao-A producing H 2 O 2 . More importantly, the proposed method has been successfully applied to the analysis of Mao-A in spiked human serum Hence, the TMBDs as the nanosubstrate provide an efficient, fast and sensitive approach for colorimetric kit of H 2 O 2 and Mao-A, promoting more extensive application in early diagnosis of disease and drug discovery.
研究领域:
分析化学
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生物化学
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环境科学
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药学
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临床化学
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DOI
高影响因子
Advanced Science
·
2023
·
IF 15.1
Long Fang,Ruofei Zhang,Lin Shi,Jiaying Xie,Long Ma,Yili Yang,Xiyun Yan,Kelong Fan
The success of arsenic in degrading PML-RARα oncoprotein illustrates the great anti-leukemia value of inorganics. Inspired by this, the therapeutic effect of inorganic selenium on t(8; 21) leukemia is studied, which has shown promising anti-cancer effects on solid tumors. A leukemia-targeting selenium nanomedicine is rationally built with bioengineered protein nanocage and is demonstrated to be an effective epigenetic drug for inducing the differentiation of t(8;21) leukemia. The selenium drug significantly induces the differentiation of t(8;21) leukemia cells into more mature myeloid cells. Mechanistic analysis shows that the selenium is metabolized into bioactive forms in cells, which drives the degradation of the AML1-ETO oncoprotein by inhibiting histone deacetylases activity, resulting in the regulation of AML1-ETO target genes. The regulation results in a significant increase in the expression levels of myeloid differentiation transcription factors PU.1 and C/EBPα, and a significant decrease in the expression level of C-KIT protein, a member of the type III receptor tyrosine kinase family. This study demonstrates that this protein-nanocaged selenium is a potential therapeutic drug against t(8;21) leukemia through epigenetic regulation.
研究领域:
肿瘤学
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分子生物学
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纳米技术
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表观遗传学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Yingjing Fan,Weilun Pan,Qi Xiu,Yue Qiao,Peng Zhang,Tong Liao,Junjie Feng,Chengtao Nie,Shuai Chu,Ningcen Li,Xiuhua Wu,Lei Zheng,Zhen Cai,Bo Li
Tumor immunotherapy has significantly improved cancer treatment, yet immune checkpoint inhibitors only benefit a subset of patients. A critical challenge is non-invasive detection of tumor PD-L1 mRNA, a key biomarker that dynamically reflects PD-L1 expression and correlates with therapeutic outcomes. Current methods are hindered by the instability of circulating mRNA and the need for invasive tissue biopsies. Herein, we introduce the Cationic LIposome capsulated CRISPR-Cas13a Kit (CLICK) for in-situ detection of circulating PD-L1 mRNA in extracellular vesicles (EVs). CLICK achieves direct target identification in 10 μL plasma within 2 hours without EV purification or RNA extraction, demonstrating a detection limit of 10 3 EVs/mL for PD-L1 mRNA. By integrating membrane fusion-mediated CRISPR delivery with nanoflow cytometry, CLICK resolves PD-L1 mRNA (+) EV subpopulations at single-vesicle resolution, revealing heterogeneous distributions. Clinically validated in 53 immunotherapy patients, CLICK accurately stratifies progressive disease (PD) from stable disease (SD) patients (AUC = 0.8236; 95% CI: 0.7054–0.9417; p= 0.0006) based on PD-L1 mRNA (+) EV percentages. This work establishes a rapid, blood-based paradigm for therapy response monitoring, advancing liquid biopsy through precise EV RNA characterization.
研究领域:
肿瘤学
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分子诊断
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转化医学
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免疫学
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RNA检测
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液体活检技术
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精准医学
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DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2013
·
IF 4.814
Bin Hu,Huan Liu,Kai Tao,Chunrong Xiong,Shenghu Zhou
Tungsten-doped mesoporous KIT-6 (W-KIT-6), mesoporous silica supported WO3/KIT-6, and traditional silica supported WO3/SiO2 catalysts have been successfully synthesized and tested for catalytic metathesis of 1-butene and ethene to propene. The resultant materials were comprehensively characterized by XRD, BET, TEM, UV–DRS, IR, XPS, H2-TPR, and TGA. For W-KIT-6 catalysts, high concentration of W5+ species by XPS and the difficulty of reduction of W species by TPR suggested the incorporation of W species into the KIT-6 framework. The studies of small-angle XRD, BET, and TEM illustrated that the 3D ordered mesoporous structure and their high surface area of KIT-6 were maintained in W-KIT-6. The doped W-KIT-6 illustrated superior catalytic performance to the supported WO3/KIT-6 and WO3/SiO2 catalysts. The origin of catalytic performance enhancement for W-KIT-6 was preliminarily discussed and was assigned to the incorporation of W species into KIT-6 framework. This study demonstrated the influence of neighboring environment of active components on catalytic performance and was helpful to design metathesis catalysts.
研究领域:
催化化学
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材料科学
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化学工程
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无机化学
·
多孔材料
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DOI
高影响因子
Chemical and Biological Technologies in Agriculture
·
2025
·Vol. 12
·期 1
·1-22
·
IF 5.2
Wu Jiahui,Yuan Yonglei,Qu Liping
As an edible algae with rich nutrients and high medicinal value, Durvillaea antarctica has certain potential in anti-aging, but there is currently limited research on the separation, purification and activity of its anti-aging active ingredients. We successfully extracted and purified a new β-(1,3)-D-glucan with a special branched structure from Durvillaea antarctica using subcritical water extraction combined with ultrafiltration membrane (SWE-UFM). The polysaccharide, named DAG-1, consists of glucose (92.70%) and has a weight-average molecular weight of 10149 Da. The GC–MS and 1D/2D NMR confirmed that DAG-1 was composed of 3)-β-D-Glcp-(1 → , → 3,6)-β-D-Glcp-(1 → , β-D-Glcp-(1 → and → 6)-α-D-Glcp-(1 → , and β-glycosidic linkages between these sugar units. Animal experiments showed that DAG-1 has significant anti-skin aging effects by inhibiting the reduction of superoxide dismutase (SOD) activity, accumulation of malonic dialdehyde (MDA), and decline in hydroxyproline (HYP) content in collagen fibers of D-gal-induced mouse skin. To further elucidate the mechanism of DAG-1, we found that DAG-1 can alleviate senescence in human dermal fibroblasts (HDFa), inhibit degranulation of human cutaneous mast cells (HCMC), and modulate inflammatory factors (TNF-α, IL-6) production. Additionally, DAG-1 may bind to c-KIT receptors and downregulate the expression of KITLG/KIT, regulating the growth and activation of mast cells, and thus alleviate skin aging. These findings suggest that DAG-1 is a potential anti-aging ingredient for functional food, cosmetic, and medicine development.
研究领域:
海洋生物学
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药理学
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功能性化妆品
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生物化学
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DOI
高影响因子
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 1.9
Sarmad Soomro,Zhenkun Sun,Dennis Y. Lu,Zhihao Zhou,Lin Li,Lunbo Duan
Catalyst deactivation due to carbon deposition and sintering poses a significant challenge to the efficient dry reforming of CH 4 and CO 2 into syngas, particularly at high Ni loadings. To tackle this difficulty, ordered mesoporous silicas with varied pore symmetries were employed as supports in this study to synthesize the Ni-based catalysts using hydrothermal treatment. The 10% Ni/MCM-41 catalyst exhibited the highest conversion rates for CO 2 (81%) and CH 4 (77%), with favourable H 2 /CO (0.92) ratios indicating efficient syngas production Higher Ni concentrations increase active sites and catalytic performance, they also increase carbon deposition, reducing catalyst durability. At lower Ni loadings (4% Ni), catalysts with a 3D pore structure showed improved activity and stability, effectively reducing carbon deposition. This study demonstrates that optimized mesopore symmetries and Ni loadings can enhance catalyst efficiency and durability for syngas production.
研究领域:
化学工程
·
材料科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ming Shang,Haiyan Wei,Ge Gao,Nianlu Li,Wei Zou,Ranran Liu,Mengping Zhang,Xiao Meng,Wenwen Chen,Yaxin Sun,Cuijuan Wang
Bromadiolone is the most frequently detected anticoagulant rodenticide in human biofluids, resulting in increased cases of poisoning. Here, we present a portable kit that could realize rapid fingerprint detection of DQ in human biofluids. This strategy was based on surface-enhanced Raman scattering (SERS) and salt-induced liquid-liquid phase separation (SILLPS) using a gold/silver core-shell nanoparticle (Au@Ag NPs) substrate. The kit included a portable Raman spectrometer and five tubes that contained Au@Ag NPs colloid, KI solution, saturated NaCl solution, acetonitrile , and Mg(NO 3 ) 2 solution. This strategy did not require pretreatment for urine samples. For blood samples, a 2-min SILLPS method was proposed with satisfactory recovery, providing unique insights into efficient pretreatment of complex biomatrix. The detection limits for urine and blood were calculated to be 3.21 ppb and 6.86 ppb, respectively. A clear trend could be observed between signal intensity and logarithmic concentration of bromadiolone ranging from 0 to 2 ppm with high reproducibility. Such a portable kit based on SILLPS-SERS can rapidly provide fingerprint information of poison. This simple-yet-practical “Pretreat & Detect” SERS kit may open up new opportunities for point-of-care detection of poisons and has great potential as a tool in field applications.
研究领域:
分析化学
·
毒理学
·
纳米技术
·
医学诊断
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产品关联
DOI
高影响因子
CLINICAL AND EXPERIMENTAL ALLERGY
·
2021
·Vol. 52
·期 1
·46-58
·
IF 5.018
Nan Wang,Jue Wang,Yongjing Zhang,Shiling Hu,Tianxiao Zhang,Yuanyuan Wu,Xiuzhen Sun,Tao Zhang,Shuanying Yang,Langchong He
Background Allergic asthma is a common inflammatory lung disease and a major health problem worldwide. Mast cells (MCs) play a key role in the early-stage pathophysiology of allergic asthma. Substance P (SP) functions in neurogenic inflammation by activating MCs, and therefore, it may to participate in the occurrence and development of asthma. Objective We examined the relationship between SP and lung inflammation, and also whether SP can directly trigger asthma. Methods We measured the number of peripheral blood eosinophils, neutrophils and basophils and evaluated the levels of IgE and SP in blood samples of 86 individuals with allergic asthma. Serum IgE and SP levels were also determined in 29 healthy individuals. C57BL/6 mice were subjected to different doses of SP, and bronchoalveolar lavage fluid (BALF) was collected to count the inflammatory cells. Lung tissues were analysed using histopathological methods to evaluate lung peribronchial inflammation, fibrosis and glycogen deposition. Levels of IgE, interleukin (IL)-1, IL-2, IL-4, IL-5, IL-13, IL-17 and IFN-γ were determined in mouse serum. Results Substance P levels were increased in the serum samples of patients with asthma. SP induced mouse lung peribronchial inflammation, fibrosis and glycogen deposition, with high levels of Th2-related cytokines such as IL-4, IL-5 and IL-13 observed in the BALF. Furthermore, low level of total IgE was noted in the serum, and SP had little effect on MC-deficient kit W-sh/W-sh mice. Conclusions & clinical relevance Substance P levels increased significantly in serum of asthmatic patients and independently associated with the risk of asthma. Furthermore, SP induced Th2 lung inflammation in mice, which was dependent on MCs. Graphical Here, we found that SP levels were increased in the serum samples of patients with asthma, which was independently associated with the risk of asthma. In mice model, SP induced lung peribronchial inflammation, fibrosis, and glycogen deposition, with high levels of Th2-related cytokines in the BALF. Furthermore, low level of total IgE was noted in the serum, and SP had little effect on MC-deficient kit W-sh/W-sh mice, which indicated that SP induced Th2 lung inflammation in mice was dependent on MCs.
研究领域:
免疫学
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肺科学
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过敏学
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分子生物学
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DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 14
·6513-6525
·
IF 6.1
Tianxu Liu,Mengting Zhang,Ismail Muhammad Asif,Yonglin Wu,Bin Li,Ling Wang
Gastrointestinal dysmotility is a common cause of functional dyspepsia. As two kinds of polysaccharides derived from brown algae, fucoidan and laminarin possess many physiological properties; however, their relative abilities in regulating gastrointestinal motility have not been illustrated yet. In this study, we aimed to investigate the regulatory effect of fucoidan and laminarin on functional dyspepsia mice induced by loperamide. Mice with gastrointestinal dysmotility were treated with fucoidan (100 and 200 mg per kg bw) and laminarin (50 and 100 mg per kg bw). As a result, fucoidan and laminarin reversed the dysfunction mainly through regulating gastrointestinal hormones (motilin and ghrelin), the cholinergic pathway, the total bile acid level, c-kit protein expression, and gastric smooth muscle contraction-related gene expression (ANO1 and RYR3). Moreover, fucoidan and laminarin intervention modulated the gut microbiota profile including the altered richness of Muribaculaceae, Lachnospiraceae, and Streptococcus. The results indicated that fucoidan and laminarin may restore the rhythm of the migrating motor complex and regulate gut microecology. In conclusion, we provided evidence to support that fucoidan and laminarin might have potential abilities to regulate gastrointestinal motility.
研究领域:
药理学
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胃肠病学
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微生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）