JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2025
·
IF 2.1
Dong-qin Bi,Shu-han Li,Xue-jia Zhang,Zi-xiao Zhao,Qi-chao Yang,Zhan-ping Qiao
Two-phase equilibrium of ternary systems of CdBr2–l-proline/(4-OH-l-proline)–H2O at T = 298.15 K is studied. Their isothermal solution equilibrium phase diagrams have been drawn based on the solubility data. Two double salts (Cd(C5H9NO2)Br2·H2O and Cd(C5H9NO3)Br2) have been found by the Schrienemakers method in phase diagrams, and they are all congruently soluble in water. Both of them have been synthesized and characterized by powder XRD and TG-DTG. For the first time, enthalpies of solution of Cd(C5H9NO2)Br2·H2O and Cd(C5H9NO3)Br2 are measured in water, and their standard molar enthalpies of formation at 298.15 K are calculated by designing thermochemical cycles according to the Hess law. By comparison, it is found that the region of infinite dissolution appears in the ternary system of CdBr2–l-proline–H2O at 298.15 K. If the composition of the solution is in this region, there is no crystal when the water evaporates and only a viscous mixture can be obtained.
研究领域:
物理化学
·
无机化学
·
热力学
·
材料科学
·
晶体学
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2020
·Vol. 22
·期 18
·5984-5988
·
IF 9.48
Yunchao Feng,Zheng Li,Sishi Long,Yong Sun,Xing Tang,Xianhai Zeng,Lu Lin
We report here that L-rhamnose, a less popular renewable and commercially available carbohydrate, can be used as a potential raw material to generate 5-methylfurfural (MF). In particular, nearly 100% yield of MF was achieved over 7 consecutive cycles in NaCl solution containing AlCl3 within 30 min, thus providing an alternative and industrializable way for the production of the potential bio-platform compound MF.
研究领域:
生物化学
·
可再生能源化学
✓ 已验证
DOI
高影响因子
MATERIALS RESEARCH BULLETIN
·
2021
·
IF 4.641
Rujia Hu,Wenjing Hou,Gaoyi Han,Ting Ou,Yunzhen Chang,Yaoming Xiao
The layered structure of perovskite solar cells determines the importance of interfaces. However, the differences in compositions and energy level always cause the undesired interface contact and defects fatally, especially for the SnO 2 /perovskite interface. These poor contacts and defects can influence the interface electron transfer and recombination dynamics. Therefore, modifying the SnO 2 /perovskite interface is necessary. Herein, we incorporate the L -cysteine as the interfacial chemical bridge between SnO 2 and perovskite. The higher electron concentration at -COOH of L -cysteine depends it can link to -OH on SnO 2 preferentially, and then the outward facing -NH 2 and -SH can coordinate with Pb, realizing the tight connection between SnO 2 and perovskite and accelerating the interface charge extraction. Besides, the coordination between L -cysteine and Pb depends that L -cysteine modification can regulate the crystallization and growth dynamics of perovskite and reduce the interface Pb defects. The performance parameters suggest that L -cysteine modification can promote the device performances.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Min Hong,Haobin Chen,Lei Li,Xiaoyan Liu,Qinghong Ji,Shuang Cheng,Chenzhong Li
A glutathione (GSH)-responsive amphiphilic camptothecin (CPT) prodrug, Lc-ss-CPT, was synthesized by conjugating hydrophobic CPT with hydrophilic L-carnitine (Lc) via a disulfide carbonate linker. The prodrug self-assembled into spherical nanoparticles (Lc-ss-CPT NPs, hydrodynamic diameter: ∼180 nm). In simulated gastrointestinal fluids and FBS-containing PBS, the NPs maintained stability over 24 h, with <10 % CPT leakage after 8 h. In vitro transcytosis studies using Caco-2 monolayers revealed an 188.5-fold higher apparent permeability coefficient for Lc-ss-CPT NPs than free CPT. The confocal fluorescence imaging studies demonstrated that the specific affinity between L-carnitine and organic cation transporter 2 (OCTN2) enhanced intestinal absorption of Lc-ss-CPT NPs and then transported into the bloodstream through transcytosis, facilitated by the Golgi apparatus and endoplasmic reticulum, enabling tumor accumulation. Pharmacokinetic analysis in rats showed oral administration of Lc-ss-CPT NPs achieved a higher bioavailability of 49.6 %. In HCT-116 cells, Lc-ss-CPT NPs reduced intracellular GSH to 5.4 % of baseline at 6 h, elevated ROS levels by 4.2-fold, and induced 71.2 % apoptosis. In MC38 tumor-bearing mice, oral Lc-ss-CPT NPs suppressed tumor growth by 86 % (vs. 12 % for free CPT). These data demonstrate the potential of Lc-ss-CPT NPs as an orally administrable platform for targeted CPT delivery.
研究领域:
药理学
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肿瘤学
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纳米医学
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS AND CORROSION-WERKSTOFFE UND KORROSION
·
2017
·Vol. 68
·期 9
·1004-1012
·
IF 1.26
P. Qiu,H. F. Yang,L. J. Yang,Z. S. Chen,L. J. Lv,Y. Song,C. F. Chen
The heteroatoms such as nitrogen, oxygen, and sulfur of organic inhibitor play a crucial role on its corrosion inhibition behavior. In order to reveal their adsorption behavior and inhibition performance under illumination condition on steel surface, this study selects L-cysteine as study object. On the basis of electrochemical measurement, surface analysis, and theoretical calculation, a promising increase in corrosion inhibition efficiency is observed when visible-light is impinged on steel surface in sodium chloride solution with L-cysteine. The enhanced performance is attributed to the sample surface photovoltaic effect, thereby facilitating both the thiol and the amino groups to tilt toward the surface.
研究领域:
材料科学
·
电化学
·
理论计算
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zhenqiang Zhao,Jiajia You,Xuanping Shi,Rongshuai Zhu,Fengyu Yang,Meijuan Xu,Minglong Shao,Rongzhen Zhang,Youxi Zhao,Zhiming Rao
Exploring effective remodeling strategies to further improve the productivity of high-yield strains is the goal of biomanufacturing. However, the lack of insight into host-specific metabolic networks prevents timely identification of useful engineering targets. Here, multidimensional engineering strategies were implemented to optimize the global metabolic network for improving l-threonine production. First, the metabolic bottleneck for l-threonine synthesis was eliminated by synergistic utilization of NADH and an enhanced ATP supply. Carbon fluxes were redistributed into the TCA cycle by rationally regulating the GltA activity. Subsequently, the stress global response regulator UspA was identified to enhance l-threonine production by a transcriptomic analysis. Then, l-threonine productivity was improved by enhancing the host’s stress resistance and releasing the inhibitory reaction of glucose utilization. Eventually, the l-threonine yield of THRH16 reached 170.3 g/L and 3.78 g/L/h in a 5 L bioreactor, which is the highest production index reported. This study provides rational guidance for increasing the productivity of other chemicals.
研究领域:
生物工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2014
·Vol. 6
·期 10
·1758-1767
·
IF 5.368
Qinghua Xu,Chaoliang He,Chunsheng Xiao,Shuangjiang Yu,Xuesi Chen
Facile synthesis of biopolymers that facilitate versatile post-polymerization modification is of great interest for biotechnological and biomedical applications. In this study, a methacryloyl-substituted L-lysine N-carboxyanhydride (LysMA-NCA) monomer was designed and synthesized, and methacryloyl-functionalized polypeptides were prepared through the ring opening polymerization (ROP) of the L-lysine-based monomer. The post-polymerization functionalization of the methacryloyl-containing polypeptides with various thiol-containing molecules was achieved with high efficiency through facile radical-mediated thiol–ene chemistry. Moreover, a block copolypeptide bearing both methacryloyl and alkynyl pendants was developed through successive ROP of LysMA-NCA and γ-propargyl-L-glutamate (PPLG-NCA). The sequential modification of the block copolypeptide with hydrophilic and hydrophobic molecules, respectively, was achieved by the successive alkyne–azido and thiol–ene “click” reactions. Overall, the facile synthesis of polypeptides bearing functional substituents and their versatile post-polymerization modification may serve as a useful platform for the development of various functional polymers.
研究领域:
生物材料学
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高分子化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
PROTEIN EXPRESSION AND PURIFICATION
·
2024
·
IF 1.4
Junzhang Chang,Yuxin Zhang,Zhiwei Li,Yunfeng Ma,Xueqin Hu,Jingwen Yang,Hongbin Zhang
Chiral amino acids and their deamination products, α-keto acids, have important applications in food, medicine, and fine chemicals. In this study, two l -amino acid deaminase genes from Proteus mirabilis , PM473 of type Ⅰ and PM471 of type Ⅱ were cloned and expressed in Escherichia coli respectively, expected to achieve the chiral separation of amino acids . Extensive substrate preference testing showed that both deaminases had catalytic effects on the d -amino acid component of the D, l -amino acids, and PM473 has a wider catalytic range for amino acids. When D, L-Cys was used as the substrate, all L-Cys components and 75.1 % of D-Cys were converted to mercapto pyruvate , and the remaining D-Cys was a single chiral enantiomer . Molecular docking analysis showed that the interaction between the substrate and the key residues affected the stereoselectivity of enzymes . The compatibility of hydrophobicity between the binding pocket and substrate may be the basic factor that affects the substrate selectivity. This work provides an alternative method for the production of α-keto acids and the resolution of chiral amino acids.
研究领域:
生物化学
·
生物催化
·
酶学
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药物化学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Wenjing Wang,Yumin He,Suxiang He,Xiaoying Liu,Qing-wen Gui,Lei Deng,Hui Wang,Zhong Cao,Zemeng Feng,Benhai Xiong,Yulong Yin
L - Tryptophan (L-Trp), one of the essential amino acids, is crucial for human physiological homeostasis and a limiting amino acid in animal feed. In addition, L-Trp is a precursor of some important biomolecules in the body, such as pentraxin and melatonin. Disorders of L-Trp metabolism in the body may cause Alzheimer's or Parkinson's disease. Therefore, monitoring L-Trp levels in the body fluids quickly and accurately is essential. A colorimetric biosensor was developed for the rapid detection of L-Trp in solutions by coupling a novel, screened, and validated peptide aptamer with Au nanoparticles via the Au-S bond. The biosensor showed a wide linear detection range of 1 µM − 1000 µM, and in addition, it was simple to construct, and the reaction time was as short as 10 s. In the analysis of L-Trp in actual porcine serum samples, the relative standard deviations were 4.52 % to 3.01 % compared with those of the high-performance liquid chromatography method, and the spiked recoveries were 99.2 % to 100.2 %. This new type of recognition probe is likely to gain attention in the field of bioanalysis owing to its biocompatibility, ease of modification, and easy linkage to nanomaterials.
研究领域:
生物化学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Weijie Gao,Zijian Jing,Yifang Meng,Qinghai Liu,Hualei Wang,Dongzhi Wei
Lysine cyclodeaminase (LCD)-mediated synthesis of l-pipecolic acid (l-PA) from l-lysine (l-Lys) is a promising approach. However, only one LCD has been reported, and its inadequate activity limits industrial applications. To address this problem, a substrate analogue-guided enzyme mining strategy was employed. A novel ornithine cyclodeaminase (OCD) from Rhizobium leguminosarum (RlOCD) was identified in combination with directed macrogenomic approaches. RlOCD displayed a conversion rate of 28% at a substrate loading as high as 1000 mM. A multiregion synergy strategy consisting of pocket reshaping, dynamical cross-correlation matrix-guided coevolutionary design, and surface modification was used to design RlOCD from the inside-out. A quadruple mutant (V93C/L119C/I170T/R90L) designated Mu4 with significantly increased activity was obtained, which showed a 28.46-fold increase in the catalytic efficiency. The conversion of Mu4 was 91% within 10 h at 1000 mM (146.19 g L–1) loading. The space–time yield of 282.1 g L–1 d–1 is the highest level ever reported. Molecular dynamics simulations and interaction analyses revealed that efficient pocket expansion and unique conformational rearrangements increased the affinity for the substrate, resulting in a more catalytically active conformation. This study expands the toolbox for the production of l-PA and demonstrates the effectiveness and potential of Mu4 for its production.
研究领域:
生物化学
·
酶工程
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Hongwei Zhao,Ling Sun,Huanhuan Chen,Lili Xiang,Dongying Chen
L-α-Glycerylphosphorylcholine (L-α-GPC) is effective to control the symptoms of cognitive decline for the patients of Alzheimer’s disease. In this study, an HPLC method coupled with a refractive index detector was developed to evaluate the intrinsic stability of L-α-GPC. The separation of L-α-GPC and its major potential degradation products was achieved on a normal-phase silica gel column (4.6 mm × 250 mm) with the mobile phase consisting of methanol–20 mM ammonium formate aqueous solution (pH 3.2) (65:35, v/v) in isocratic mode. The HPLC method was validated satisfactorily with respect to precision, accuracy and robustness. It is found that L-α-GPC is stable under the photolytic, thermal, oxidative and acidic conditions, while relatively sensitive to alkaline condition due to the specific breakage of phosphate ester bond in the moiety of L-α-GPC. A preliminary kinetics study for the alkaline degradation was conducted with the corresponding kinetics parameters obtained. It can be concluded that the developed HPLC method is capable of distinguishing the stability difference between the two phosphate ester bonds characterized on the L-α-GPC chemical structure.
研究领域:
药学
·
分析化学
·
神经科学
✓ 已验证
DOI
高影响因子
FIBERS AND POLYMERS
·
2017
·Vol. 18
·期 11
·2204-2211
·
IF 1.113
Xu QingBo,Gu JiaYuan,Zhao Yang,Ke XiaTing,Liu XiangDong
L-cysteine (Cys) and silver nanoparticles (Ag NPs) were successfully linked onto the cotton fabric surfaces. The Cys molecules were covalently linked to the cotton fibers via esterification with the cellulose hydroxyl groups, and the Ag NPs tightly adhered to the fiber surface via coordination bonds with the Cys thiol groups. As a result, the Ag NPs coating on the cotton fabric showed an excellent antibacterial function with an outstanding laundering durability. The bacterial reduction rates (BR) efficiency reached 100 % for Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). After 50 consecutive laundering cycles, the bacterial reduction rates (BR) against E. coli and S. aureus were maintained over 97 %. It has potential applications in a wide variety of fields such as sportswear, socks, and medical textile.
研究领域:
材料科学
·
生物医学工程
·
纺织科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）