Frontiers in Bioscience-Landmark
·
2024
·Vol. 29
·期 2
·83
·
IF 3.3
Linbang Wang,Xinyu Dou,Linzhen Xie,Xuchang Zhou,Yu Liu,Jingkun Liu,Xiaoguang Liu
Background: Lactic acid, previously regarded only as an endpoint of glycolysis, has emerged as a major regulator of tumor invasion, growth, and the tumor microenvironment. In this study, we aimed to explore the reprogramming of lactic acid metabolism relevant to osteosarcoma (OS) microenvironment by decoding the underlying lactic acid metabolic landscape of OS cells and intercellular signaling alterations. Methods: The landscape of OS metabolism was evaluated using single-cell gene expression data, lactic acid metabolism clustering, and screening of the hub genes in lactic acid metabolism of OS samples using transcriptome data. The role of the hub gene NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 6 (NDUFAF6) was validated with in vitro studies and patient experiments. Results: Single-cell RNA sequencing data validated a lactic acid metabolismhigh subcluster in OS. Further investigation of intercellular communications revealed a unique metabolic communication pattern between the lactic acid metabolismhigh subcluster and other subclusters. Next, two lactic acid metabolic reprogramming phenotypes were defined, and six lactic acid metabolism-related genes (LRGs), including the biomarker NDUFAF6, were screened in OS. In vitro studies and patient experiments confirmed that NDUFAF6 is a crucial lactic acid metabolism-associated gene in OS. Conclusions: The patterns of lactic acid metabolism in OS suggested metabolic reprogramming phenotypes relevant to the tumor microenvironment (TME) and identified NDUFAF6 as an LRG prognostic biomarker.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Yanke Zhang,Chi Zhang,Wei Chen,Zhengchun Liu
The detection of glucose in beverages is crucial for dietary management and healthcare. Excessive sugar consumption can lead to severe conditions, such as obesity, diabetes, cardiovascular diseases, and cancer. In this study, Cu-embedded laser-induced graphene (Cu/LIG) materials were fabricated for non-enzymatic glucose detection using a simple, direct, and cost-effective laser-patterning method on a Cu 2+ /ethyl-cellulose/polyimide membrane. The experimental results showed that the Cu/LIG electrode exhibited lower charge transfer resistance and a distinct diffusion process compared to the LIG electrode. This improvement can be attributed to the optimized graphene formation achieved by employing ethyl-cellulose (EC) and CuCl 2 as combustion promoters. Under optimal conditions, Cu/LIG-600 demonstrated excellent electrocatalytic activity for glucose oxidation, with a low response concentration (5 μM), fast response time (2.0 s), and detection ranges of 0.005–0.525 mM (I-t) and 0.5–4.0 mM (CV). Moreover, the sensor exhibited high selectivity, stability (retaining over 90 % activity after 28 days), and satisfactory recovery rates for spiked glucose in Cola, Sprite, Black Tea, and sugarless Soda Water, indicating its potential for non-enzymatic glucose detection in beverages.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jiamin Luo,Heng Lu,Bao-Ying Wen,Qiaowen Zheng,Junying Zhang,Yishan Li,Wenping Hong,Shanshan Zhao,Libo Shun,Feiming Li,Zhixiong Cai,Jin-Ming Lin,Qiang Chen,Maosheng Zhang,Jian-Feng Li
Methyl iodide (CH3I) gas as a toxic gas causes great harm to organisms due to its high volatility and high reactivity with biological nucleophiles. Unfortunately, the sensing and detection of CH3I gas are challenging because of the diffusive nature of the gases and its low concentrations in the environment. Herein, we have developed a fast, green, and sensitive CH3I gas visual sensing method based on the capture technology of toxic gases by natural deep eutectic solvents (NADESs) coupled to the halide rapid exchange capability of perovskite nanocrystals (PNCs). In this strategy, NADESs are used as an absorption solution to adsorb gaseous CH3I, while simultaneously exposing I– through the action of the nucleophilic reagent; then, CsPbBr3 PNCs were synthesized in NADESs and used as sensing material to achieve I– exchange. Benefiting from the capture and enrichment of CH3I gas, the sensitivity of the gas sensor was highly improved. The sensor exhibited the lowest detection limit (limits of detection) of 164.15 μmol/m3, below the minimum safe level for human inhalation, which is 200 μmol/m3. This breakthrough offers greater possibilities for the quantitative detection of CH3I gas.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Peng Gan,Kai Zhang,Qixi Xu,Yu Zhao,Jinze Li,Guihua Yang,Yunlai Zhang,Yifan Wang,Lei Zhang,Baobin Wang,Minghui Shang,Jiachuan Chen
The development of non-waste biorefinery technology is conducive to the efficient utilization of waste biomass resources. In this study, the microwave-assisted deep eutectic solvent (DES) was used to pretreat sugarcane bagasse for efficient separation of chemical components and upgrading each component into industrial products, including paper, lignin microspheres (LMS) and furfural . After rapid and mild DES pretreatment at 135 °C for 20 min, the retention rate of cellulose in the solid residue was 95.2 %, while the removal rates of hemicellulose and lignin were 84.1 % and 88.3 %, respectively. The whiteness of handmade paper derived from solid residue could reach 63.8 %ISO. During DES fractionation, the gradual increase of water reduced the collision probability of lignin fragments, inhibited condensation reactions , and achieved graded precipitation of lignin. Then, the self-assembly method enabled the fabrication of both small solid LMS and large hollow LMS. Furthermore, AlCl 3 was added to the diluted DES to convert the separation system into a catalytic system. Under the synergistic catalysis of lactic acid, AlCl 3 , and choline chloride, biomass sugars were efficiently converted into furfural with a yield of 68.81 %. In a word, the high-value utilization of all components of sugarcane bagasse was achieved through a sustainable biorefining process, resulting in an impressive comprehensive utilization rate of 70.6 %.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Hailiang Sheng,Rongli Li,Rui Li,Long Li,Shizhao Li,Yunqi Li,Jili Yuan,Jun Huang,Qinqin Xu,Qiang Zheng,Lihua Zhang,Haibo Xie
Polymeric conductive hydrogels have been highlighted for use in flexible electronics, and there is still a challenge to design and fabricate a high-performance hydrogel electrolyte with integrated and multiple properties, such as sustainability, stretchability and compressibility, ionic conductivity, antibacterial property, strain sensitivity, and low-temperature tolerance. Herein, chitosan (CS) derived poly(aprotic/protic ionic liquids) (CS-PAPILs) are facilely prepared by taking the structural and sustainable features of chitosan and betaine hydrochloride, which are used as a functional component for the fabrication of CS-PAPILs/polyacrylamide (PAM)/LiCl (CS-PAPILs/PAM/LiCl) double-network (DN) hydrogel electrolyte via an in situ polymerization of AM and then a soaking strategy in LiCl aqueous solution. The achieved DN hydrogels exhibit tunable mechanical performance (tensile strength of 70–900 kPa, elastic modulus of 31.5–484 kPa, high compressibility (4450 kPa at 80 % strain), superior low-temperature tolerance (freezing point < -85 °C), anti-dehydration performance, high ionic conductivity at 25/-50 °C (94.8/4.6 mS cm −1 ), and excellent antibacterial activity. As a proof of concept, an assembled flexible and anti-freezing supercapacitor by the as-prepared DN hydrogel electrolyte demonstrated a high specific capacitance of 108.4F/g (at 2 A/g) and impressive cycling stability over 50,000 cycles at temperature as low as −50 °C. Furthermore, a wearable strain sensor based on the DN hydrogel was also demonstrated, and the sensor can be successfully attached to the human joints to monitor various human motions.
✓ 已验证
产品关联
DOI
高影响因子
Matter
·
2024
·
IF 17.3
Pingping Fan,Kui Li,Tian Li,Panke Zhang,Shuo Huang
Alcoholic beverages, such as wine, beer, and distilled spirits, are widely produced and consumed in different nations. Different types of alcoholic beverages contain different combinations of flavor compounds. However, rapid and simultaneous analysis of a large variety of compounds in alcoholic beverages by a miniatured and portable device remains a challenge. In this paper, a Mycobacterium smegmatis porin A (MspA) nanopore modified with a phenylboronic acid (PBA) adapter is applied for rapid analysis of a variety of alcoholic beverages. By utilizing custom machine learning algorithms, various cis -diols are identified simultaneously in both distilled and fermented alcoholic beverages, generating unique barcodes for each sample type. Nanopore analysis of alcoholic beverages has also never been carried out previously. Rapid grading of wine sweetness and detection of additives, including sucrose and D-tartaric acid, are also demonstrated, showcasing the significance of this technique in the administration of wine production.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Cun-Duo Tang,Hong-Ling Shi,Jian-He Xu,Zhu-Jin Jiao,Fei Liu,Peng-Ju Ding,Hong-Fei Shi,Lun-Guang Yao,Yun-Chao Kan
d-Mandelate dehydrogenase (DMDH) has the potential to convert d-mandelic acid to phenylglyoxylic acid (PGA), which is a key building block in the field of chemical synthesis and is widely used to synthesize pharmaceutical intermediates or food additives. A novel NAD+-dependent d-mandelate dehydrogenase was cloned from Lactobacillus harbinensi (LhDMDH) by genome mining and expressed in Escherichia coli BL21. After being purified to homogeneity, the oxidation activity of LhDMDH toward d-mandelic acid was approximately 1200 U·mg–1, which was close to four times the activity of the probe. Meanwhile, the kcat/Km value of LhDMDH was 28.80 S–1·mM–1, which was distinctly higher than the probe. By coculturing two E. coli strains expressing LhDMDH and LcLDH, we developed a system for the efficient synthesis of PGA, achieving a 60% theoretical yield and 99% purity without adding coenzyme or cosubstrate. Our data supports the implementation of a promising strategy for the chiral resolution of racemic mandelic acid and the biosynthesis of PGA.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jiatao Zhang,Bowen Guan,Yuan Zhang,Jian Hu,Tao Sun,Tungalag Dong,Xueyan Yun
An new high-barrier packaging films for meat preservation were obtained by depositing graphene oxide (GO) layer-by-layer (LbL) on poly( l -lactic acid)(PLLA)-poly (butylene itaconate) (PBI) copolymers (PLBI) with chitosan (CS) as bonding layer in this study. The 1 H NMR results revealed that PLBI copolymer was successful synthesized. The introduction of PBI reduced the T g and crystallization capacity of PLLA film, which made the copolymer films exhibit extremely high flexibility (up to 186.1% elongation at break). The film also exhibited high hydrophilicity, which was favorable for the effective attachment of CS. With CS as an intermediate layer, the FTIR and SEM results showed that the GO was evenly distributed onto the PLBI film surface due to the electrostatic interaction. The oxygen, water vapor and UV light barrier properties of PLBI/CS/(GO-CS) x films were improved and increased with the GO-CS layers. The PLBI/CS/(GO-CS) 10 films, with extremely low oxygen permeability coefficients about 0.4 × 10 −8 cm 3 m/m 2 ·h·Pa, delayed the increase in weight loss, hardness, pH, TVB-N, TBARS, and total viable counts of chilled meat during storage. It also decreased the discoloration, and loss of springness and cohesiveness of chilled meat caused by long-term refrigeration. The LbL films even showed better freshness-keeping effect than commercial PA/PE films due to its superior barrier and certain antibacterial properties, suggesting great potential application in fresh meat preservation.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2025
·
IF 3.6
Jiatao Zhang,Tungalag Dong,Yuan Zhang,Jia Guo,Yulu Li,Xueyan Yun
Although poly(L-lactic acid) (PLLA) is biocompatible and degradable, its inherent brittleness, susceptibility to aging, low ultraviolet light (UV) resistance, and moderate gas barrier properties limit its diverse applications. In this study, graphene oxide (GO) was used as an initiator, unsaturated poly (butylene itaconate) (PBI) was used as a flexible chain segment, and GO-grafted P(LA- g -BI) (GO- g -PLBI) copolymer was synthesized by in situ melt polycondensation of lactic acid. Nuclear magnetic resonance, Fourier transform infrared, and X-ray photoelectron spectroscopy results confirmed the successful synthesis of GO-g-PLBI and the formation of a multi-branched structure. The GO- g -PLBI film demonstrated excellent ductility and oxygen barrier properties, with elongation at break increased by 73.8 times compared with PLLA, while oxygen permeability (OP) decreased by 40.3%. The incorporation of PBI facilitated micro-crosslinking within the copolymer, thereby enhancing its thermal stability. More gg and gt conformations were formed in the copolymer, while the crystallization of the copolymer induced by aging was inhibited, thus showing excellent anti-aging properties. Interestingly, the OP value of this micro-crosslinked GO-g-PLBI film decreased by 60.9% after physical aging, demonstrating extremely high oxygen barrier properties. This study offers a viable approach to developing PLLA/graphene nanocomposites that exhibit adjustable flexibility, enhanced oxygen barrier, heat and UV resistance, anti-aging properties, and high transparency. Graphical
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jian Hu,Xueyan Yun,Yan Zheng,Tao Sun,Lijun Song,Pengju Pan,Tungalag Dong
High costs and low performance have constrained the application of bio-based materials in food packaging. Herein, a series of ultra-thin poly(L-lactic acid-iconic acid N-diol) (P(LA-NI)) copolymer films were developed using a “one-step” polycondensation process with integrated toughness, barrier properties, gas selectivity, and quality control features. The massive branched structure and gg conformers in P(LA-NI) act as “internal chain expansion” and “internal plasticization”. Meanwhile, P(LA-NI) contains numerous polar groups and unique nanoscale microphase structures to realize excellent CO 2 , O 2 barrier, CO 2 /O 2 selectivity, anti-fogging, and UV shielding functions. The atmosphere within the package spontaneously achieves the desirable low O 2 and high CO 2 levels when packaging button mushrooms with high respiratory metabolism. Eventually, the shelf life of button mushrooms reached 24 days, >3-fold extended. This PLLA-based film meets “dual carbon” and “food safety” goals and has vast potential for fresh food preservation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemCatChem
·
2025
·
IF 3.9
Long Zhang,Linjie Guan,Jiashu Liu,Song Song,Ye Tian,Xingang Li
Graphical N-ZSM-5–40 nanosheet zeolites prepared by seed-induced growth coupled with mineralization agent assisted method exhibited remarkable activity and stability in conversion of lactic acid to lactide. Enhanced diffusion and accessible Brønsted acid sites amount benefit the production of lactide. Lactide (LD) is the key building block of high-quality polylactic acid (PLA), a biodegradable polymer with broad industrial applications. Developing an efficient catalyst for one-step conversion of lactic acid to LD is critical for the PLA industry. Herein, N-ZSM-5–40 nanosheets with a thickness of about 20 nm were successfully synthesized and first applied to this esterification reaction. The yield of LD over N-ZSM-5–40 reaches 60.5% after reaction at an oil bath temperature of 140 °C for 5 h. Systematic characterization revealed that reducing the b-axis thickness of MFI-type nanosheet zeolites improves Brønsted acid sites (BASs) accessibility, which was identified as a critical factor governing the yield of LD. Time-dependent product distribution experiments demonstrate that the nanosheet structure of N-ZSM-5–40 facilitates faster cyclization of L 2 A to LD, compared to commercial C-ZSM-5–40. In-situ FTIR results reveal that N-ZSM-5–40 nanosheets have a strong ability to adsorb and activate carbonyl groups, which promotes the rapid conversion of lactic acid to LD. Given the aforementioned advantages, ZSM-5 nanosheets hold great potential for guiding the development of industrial catalysts for sustainable LD synthesis.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Qing Li,Congnan Wu,Jiaxing Zhang,Rongxin Su,Yuefei Wang,Wei Qi
The development of enantioselective nanozymes holds profound significance in synthesizing chiral compounds and elevating analytical precision. Herein, we have synthesized chiral gold/iron hierarchically organized particles (Cys@Au/Fe Hops) possessing peroxidase-like catalytic activity, through a one-pot method using cysteine as a chiral inducer. The nanozymes exhibited remarkable enantioselective catalytic activity to 3,4-dihydroxy-phenylalanine (DOPA) enantiomer. Notably, L -Cys@Au/Fe Hops, comprising right-handed helical nanopetals, exhibited elevated catalytic efficiency for L -DOPA over D -DOPA, whereas the reverse held for D -Cys@Au/Fe Hops. The molecular dynamics simulations demonstrated hydrogen bonding interactions between the active site and substrate, thereby revealing how steric effects affected the enantioselective catalysis of chiral DOPA by L/D -Cys@Au/Fe Hops. Moreover, the chiral nanozymes were developed into a test paper sensor due to their exceptional stability, which can achieve the visual detection of DOPA enantiomer combined with a smartphone platform. This work is significant in advancing the design and practical utility of highly selective enzyme mimics.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）