BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xiangqin Meng,Sijia Zou,Dandan Li,Jiuyang He,Ling Fang,Haojue Wang,Xiyun Yan,Demin Duan,Lizeng Gao
The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS 2 nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS 2 nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS 2 nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections.
研究领域:
分子生物学
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病毒学
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即时诊断
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纳米技术
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2024
·
IF 1.3
Haifan Chen,Gongwei Xia,Jianfang Chen,Hao Zheng
Phenolic compounds are considered to be major environmental pollutants because of their toxicity and hazard on the marine ecosystem and human health even at low concentrations. An ultrasensitive biosensor was fabricated by immobilizing polyphenol oxidase into a composite of transition metal carbides (MXene)-yttrium oxide (Y 2 O 3 ) for the detection of catechol in environmental water bodies. The morphology and electrochemical performance of the as-prepared biosensors were investigated using scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV), respectively. Under the optimal pH value and applied potential, the biosensor exhibited a linear response range from 0.04 to 3.2 µM (R 2 = 0.9974) with an excellent sensitivity of 2294.8 mA·M −1 . The limit of detection (LOD) was found to be 7 nM (S/N = 3) with acceptable repeatability and reproducibility. The interference from ascorbic acid, uric acid, CuSO 4 , MgSO 4 , FeCl 3 , CaCl 2 , and ZnSO 4 was also investigated. The proposed biosensor was used for the determination of trace catechol in real water samples with satisfactory results compared with that of HPLC. The construction of MXene-Y 2 O 3 composite is expected to be a platform for immobilizing enzymes to prepare versatile electrochemical biosensors.
研究领域:
环境科学
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分析化学
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材料科学
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电化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Miao Zhang,Haowei Zhang,Yunlei Zhou,Huanshun Yin,Zhengkun Yu,Xinyue Zhang,Shiyun Ai,Minghui Wang
The OPECT biosensing platform, which connects optoelectronics and biological systems, offers significant amplification and more possibilities for research in biological applications. In this work, a homogeneous organic photoelectrochemical transistor (OPECT) biosensor based on a Bi2S3/Bi2MoO6 heterojunction was constructed to detect METTL3/METTL14 protein activity. The METTL3/METTL14 complex enzyme was used to catalyze adenine (A) on an RNA strand to m6A, protecting m6A-RNA from being cleaved by an E. coli toxin (MazF). Alkaline phosphatase (ALP) catalyzed the conversion of Na3SPO3 to H2S through an enzymatic reaction. Due to the adoption of the strategy of no fixation on the electrode, the generated H2S was easy to diffuse to the surface of the ITO electrode. The Bi2S3/Bi2MoO6 heterojunction was formed in situ through a chemical replacement reaction with Bi2MoO6, improving photoelectric conversion efficiency and realizing signal amplification. Based on this “signal on” mode, METTL3/METTL14 exhibited a wide linear range (0.00001–25 ng/μL) between protein concentration and photocurrent intensity with a limit of detection (LOD) of 7.8 fg/μL under optimal experimental conditions. The applicability of the developed method was evaluated by investigating the effect of four plasticizers on the activity of the METTL3/METTL14 protein, and the molecular modeling technique was employed to investigate the interaction between plasticizers and the protein.
研究领域:
生物化学
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生物传感器
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光电化学
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蛋白质活性检测
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Shi Qiaoqiao,Huang Jie,Sun Yaning,Deng Ruiguang,Teng Man,Li Qingmei,Yang Yanyan,Hu Xiaofei,Zhang Zhijun,Zhang Gaiping
The authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for QNS), (b) surface-enhanced Raman scattering (SERS) detection, and (c), a portable lateral flow assay (LFA). The two probes consist of gold nanoparticles (AuNPs) conjugated to the Raman active molecule 4-aminothiophenol (PATP), and to monoclonal antibody against NEO (NEO mAb) or against NOR (NOR mAb). Quantitative detection of NEO and QNS was realized via SERS of the PATP-coated AuNPs captured in the test line of a LFA. Under optimized condition, the visual limits of LFA are 10 ng·mL −1 for NEO and 200 ng·mL −1 for NOR, and with LODs down to 0.37 pg·mL −1 and 0.55 pg·mL −1 by using SERS. The NEO test line is not interfered by the NEO analogues gentamycin, streptomycin and tobramycin, but the NOR test line suffers from different degrees of cross-reactivity (CR) to 12 common other QNS, the CRs ranging from 1.5% to 136%. The recoveries of NEO and NOR from spiked milk samples ranged between 86% and 121%, with relative standard deviations (RSD) from 3% to 6%. The method is highly sensitive, accurate and effective. It may be applied to simultaneous detection of NEO and 8 QNS, including NOR, enoxacin, ciprofloxacin, ofloxacin, fleroxacin, marbofloxacin, enrofloxacin, and pefloxacin.
Schematic of a lateral flow assay (LFA) based on an indirect competitive model. By using two test lines, the LFA can detect the neomycin and quinolones antibiotics simultaneously. Based on the surface-enhanced Raman scattering (SERS), the LFA shows high sensitivity to antibiotics with low limit of detection.
研究领域:
分析化学
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纳米技术
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药物分析
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食品安全
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2023
·
IF 5
Wenjing Qian,Jingming Zhou,Yumei Chen,Hongliang Liu,Peiyang Ding,Yankai Liu,Chao Liang,Xifang Zhu,Ying Zhang,Enping Liu,Aiping Wang,Gaiping Zhang
Virginiamycin (VIR), a feed additive, is used to promote pig and poultry growth. However, it is hazardous to human health. This work described a label-free electrochemical immunosensor based on silver nanoparticles-reduced graphene oxide (AgNPs-rGO) nanocomposites and staphylococcal protein A (SPA) for the first time to directly detect the residual marker VIR M1. Good catalytic currents for oxygen reduction reaction were apparently obtained after the modification of nanocomposites on gold electrode. Nanocomposites were characterized using UV–Vis, X-ray diffraction (XRD) patterns, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). SPA was targeted to immobilize VIR M1 monoclonal antibody (mAb) by binding to Fc region of antibody. The proposed immunosensor showed a wide linear range from 0.25 ng mL −1 to 100 ng mL −1 , providing detection limit (LOD) of 0.18 ng mL −1 of VIR M1. Recovery rates ranged from 92.27% to 98.84%, and relative standard deviation (RSD) was not above 6.6%, indicating the immunosensor could detect VIR M1 in actual samples with high accuracy. The sensor showed good selectivity, reproducibility and stability and could be considered as a potential tool for detection of VIR M1 in feed and animal derived food.
研究领域:
分析化学
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电化学
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生物传感器
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食品安全
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yaqi Yang,Jiejun Li,Zhiwang Luo,Xingnian Li,Jiayi Zou,Chunyan Li,Chao Chen,Yixi Xie,Pengcheng Zhao,Junjie Fei
One-dimensional (1D) ordered mesoporous carbon hybrid nanotubes loaded with bimetallic oxide hollow nanospheres are expected to be very attractive electrocatalysts . In this study, through high-temperature pyrolysis , we generated Mo 2 C@C PNTs with outstanding electrical conductivity , a significant surface area , and effective catalytic activity by wrapping ordered mesoporous polypyrroles on the surface of 1D MoO 3 nanotubes using block polymers that self-assembled. FeMnO 3 hollow porous nanospheres have a large specific surface area, many electroactive sites, and excellent molecular/charge diffusion properties, which can be compounded with 1D ordered mesoporous nitride-rich carbon to solve the problem of poor electrical conductivity of metal oxides and to prevent the accumulation of 1D nanotubes. The composite showed excellent electrocatalytic activity towards rutin due to the synergistic interaction between Mo 2 C@C PNTs and FeMnO 3 . In the best case, rutin has a limit of detection (LOD) as low as 0.35 × 10 −9 M and a wide linear range (3 × 10 −9 to 3.1 × 10 −6 M). The sensor displays a remarkable level of selective response to rutin in the presence of other co-interfering species. Furthermore, it has superb repeatability and reproducibility. The sensor has shown outstanding recovery (95.7–104.1 %) in the practical viability of detecting rutin in beverages. This work not only provides a new candidate for the accurate detection of rutin but also provides insight into the exploration of advanced sensing materials.
研究领域:
材料科学
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电化学
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纳米技术
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分析化学
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传感器
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Tianyu Zeng,Shuai Wu,Qizhi Liang,Hai Shi,Jue Gong,Ningjun Duan,Wei Li,Genxi Li,Yongmei Yin
The emergence of antibody-drug conjugates (ADCs) targeting trophoblast cell-surface antigen-2 (Trop-2) has reshaped the therapeutic landscape of advanced breast cancer. Accurate profiling of the Trop-2 status of tumor cells can facilitate the identification of patients who will benefit from Trop-2-targeting therapy; however limited analytical method has hindered this process. In this study, we have proposed a specific and sensitive biosensor for visual tracking of the Trop-2 status of breast cancer cells based on tetrahedral DNA nanostructure (TDN)-decorated Fe-based metal-organic framework nanoparticles (TDN-PCN-222 (Fe)). In the design, a dual-aptamer-assisted biomimetic capture strategy shows high capture efficiency while maintaining the viability and original phenotype of captured cells, ensuring the accurate profiling of the Trop-2 status. Meanwhile, by using the high intrinsic peroxidase activity and excellent targeting ability of Trop-2-specific aptamer-linked TDN-PCN-222 (Fe), specific detection of Trop-2-positive tumor cells can be achieved with a limit of detection (LOD) of 10 cells/mL, and the Trop-2 status of tumor cells can be visually tracked. Moreover, the proposed biosensor has been successfully used for tracking the Trop-2 status of tumor cells in breast cancer tissues, suggesting that our method has great promise for clinical applications.
研究领域:
生物技术
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癌症生物学
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纳米技术
·
分子诊断
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 20
·13824-13833
·
IF 3.9
Yani Zheng,Yin Dai,Junqiang Hong,Huizhu Fan,Qing Zhang,Wei Jiang,Wei Xu,Jianwen Fei,Junli Hong
The compound 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), one of the tobacco specific nitrosamines (TSNAs), is widely recognized as a major carcinogen found in tobacco products, environmental tobacco smoke and wastewater. Thus, a selective enrichment and sensitive detection method for monitoring the risk of NNK exposure is highly desirable. In this study, a magnetic molecularly imprinted polymer (MMIP) functionalized with dendritic nanoclusters was synthesized to selectively recognize NNK via the dummy template imprinting strategy, aiming to avoid residual template leakage and increase the imprinting efficiency. The nanocomposites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, as well as vibrating sample magnetometry (VSM) and nitrogen adsorption/desorption analysis. The resulting MMIPs exhibited high adsorption capacity, fast binding kinetics and good selectivity for trace amounts of NNK. A rapid, low cost and efficient method for detecting NNK in tobacco products was established using magnetic dispersive solid-phase extraction coupled with HPLC-DAD with a good linear range of 0.1–250 μg mL−1. The limit of detection (LOD) and limit of quantification (LOQ) of NNK were 13.5 and 25.0 ng mL−1, respectively. The average recoveries were 87.8–97.3% with RSDs lower than 3%. The results confirmed that the MMIPs could be used as an excellent selective adsorbent for NNK, with potential applications in the pretreatment of tobacco products.
研究领域:
分析化学
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环境科学
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烟草科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ziwei Hu,Xia Zhou,Jun Duan,Xueqiang Wu,Jiamin Wu,Pengcheng Zhang,Wanzhen Liang,Jialiang Guo,Huaihong Cai,Pinghua Sun,Haibo Zhou,Zhengjin Jiang
The design of a rapid, simple, and ultrasensitive detection platform of protein biomarkers has become an urgent need in early diagnosis and treatment. Surface-enhanced Raman scattering (SERS) technology, with the unique advantages of ultra-sensitivity and fingerprint recognition, has attracted much attention in biomarker detection. An innovative aptamer SERS biosensor for highly sensitive protein biomarker detection was presented. This biosensor contains a SERS tag (reporter-labeled Au nano-bridged nanogaps particles, Au NNPs) and novel magnetic capture substrate (Ag-coated Fe 3 O 4 -Au NPs, Ag MNPs). C-reactive protein (CRP), a representative biomarker for diagnosing, was used to assess the SERS performance of this biosensor. Aptamers against CRP were modified on both SERS tag and magnetic capture substrate for specific recognition by Au NNPs-CRP-Ag MNPs sandwich assay. This strategy achieved sensitive detection of CRP with a limit of detection (LOD) of 10 fM (1.14 pg/mL), and is much lower than reported analytical methods. Besides, this method exhibits excellent selectivity and specificity for CRP under the interference of other proteins and displays high accuracy in the detection of actual human serum samples. This proposed detection strategy can be extended to the sensitive detection of other biomarker proteins and hold great promise in the clinical diagnosis of diseases.
研究领域:
生物传感
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纳米技术
·
分析化学
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生物医学工程
·
诊断医学
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产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 36
·期 6
·681-685
·
IF 1.618
Liu Wenwen,Ma Ligang,Guo Zhuangzhuang,Liu Tao,Liu Yanju,Wang Dazhong,Kong Jinming
We propose a novel, ultrasensitive and low-cost sensor using reversible addition-fragmentation chain transfer (RAFT) polymerization as a signal amplification strategy for the detection of CYFRA 21-1 DNA fragment, a tumor marker of non-small cell lung carcinoma. The peptide nucleic acid (PNA) probes were firstly immobilized on magnetic beads (MBs) to capture the CYFRA 21-1 DNA specifically. After hybridization, CPAD was tethered to the hetero duplexes through carboxylate-Zr 4+ -phosphate chemistry. Subsequently, a number of fluorescent tags were introduced to the heteroduplexes through RAFT polymerization, leading to an amplification of the fluorescence signal. The sensor demonstrates a low limit of detection (LOD) of 0.02 fM. It has great selectivity with respect to base mismatch DNA, and high anti-interference ability in normal human serum. Overall findings of the study suggest that proposed sensor holds enormous potential to be used as a tool for the early-stage diagnosis of lung cancers.
研究领域:
生物技术
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癌症诊断
·
纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Dan Wang,Guanchen Xu,Xingshuang Zhang,Hongyu Gong,Li Jiang,Guanliang Sun,Yu Li,Guoran Liu,Yong Li,Shikuan Yang,Xiu Liang
A novel approach is developed to fabricate an ultra-thin layered flexible wearable Surface-Enhanced Raman Scattering (SERS) substrate named silk fibroin-anodic aluminum oxide-Au nanoparticles (SF-AAO-Au), which consists of biocompatible SF film as flexible support, ultrathin cavity AAO as template, and plasmonic Au NPs. The SF-AAO-Au sensor exhibits excellent SERS mechanical flexibility, homogeneity and sensitivity for its effective enhancement ability and highly ordered 3D periodic porous particle-in-cavity (PIC) arrays structure. The humidity-driven interface structure of biocompatible SF film provides good skin adhesion under various fitness tests and excellent mechanical flexibility as a wearable SERS sensor on natural skin (deformation within 5%). The flexible SF-AAO-Au can be developed as quantitative sweat glucose sensor in the concentration range of 10 − 7 to 10 − 3 M with a limit of detection (LOD) of 1.68 × 10 − 7 M and qualitative human sweat glucose detection ability. This SF-AAO-Au substrate can be further designed as lab-on-glove SERS sensing platform for ultrasensitive (LOD ~5.7 ppt) quantitative on-site detection of agricultural residues in a wide range. The effective dual-functional SERS wearable sensor realized for the first time paves a new way to develop smart wearable and non-destructive devices in environmental safety, forensic identification and point-of-care testing (POCT) diagnosis of physiological disease.
研究领域:
材料科学
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纳米技术
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可穿戴技术
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分析化学
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生物医学工程
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环境科学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xi Chen,Qian Huang,Shuyan Ruan,Feili Luo,Ruiyun You,Shangyuan Feng,Lanjin Zhu,Yang Wu,Yudong Lu
Hyaluronidase expression is known to be upregulated in various pathological conditions. A method based on a combination of ratiometric surface-enhanced Raman scattering (SERS) and magnetic separation is described for the determination of hyaluronidase (HAase) activity. Gold nanospheres (AuNPs) functionalized by 4-mercaptophenylboronic acid (4-MPBA) form stable cyclic esters with diol on hyaluronic acid (HA) by the boronic acid group, while Fe 3 O 4 @ [email protected] modified with mercaptoethylamine (MEA) was used as a capture substrate to bind to the carboxyl group on the surface of HA, forming the “ [email protected] @HA/Fe 3 O 4 @ [email protected] @MEA” “core-satellite” structure. When HAase is present, HA is enzymatically disrupted, resulting in the destruction of the “core-satellite” structure, the SERS signal of 4-MPBA is subsequently weakened. The gold shell in the substrate protects the 5,5′-Dithio bis-(2-nitrobenzoic acid) (DTNB) from the external environment, which makes it become an ideal internal standard (IS) molecule for subsequent calibration. Under optimal conditions, the I 1075 /I 1324 varied in the range of 10 −3 - 10 U‧mL −1 HAase activity, with a limit of detection (LOD) of 0.32 mU‧mL −1 ，below the level of HAase in normal human body fluids. This method has been successfully applied to the determination of HAase activity in urine and is expected to provide a new method in disease detection, especially in the non-invasive detection of bladder cancer.
研究领域:
分析化学
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纳米技术
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生物标志物检测
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癌症诊断
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）