CARBOHYDRATE POLYMERS
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2025
·
IF 12.5
Yue Ming,Zhuangzhuang Shi,Yufei Zhao,Jue Li,Wei Sha,Weilong Wang,Guoqiang Li,Mengmeng Wu,Ting Ma
The oral delivery of probiotics faces numerous challenges, particularly the acidic gastric environment. This study aimed to construct microcapsules using acid-resistant polysaccharide sanxan gum (SG) combined with sodium alginate (SA) and chitosan (CS), and to evaluate their protective effects on the probiotic bacteria Lactobacillus plantarum ( Lp ) during in vitro simulated gastrointestinal digestion. Sanxan gum, an edible polysaccharide produced by Sphingomonas sanxanigenens strain, is composed of mannose, glucuronic acid, rhamnose, and glucose. Sodium alginate has been widely used to protect probiotics because it is cheap, safe, and easy to form into microcapsules. Chitosan is a cationic polysaccharide with excellent mucoadhesive properties and film-forming capabilities. Herein, the microcapsules were prepared using the extrusion drop method. Compared with microcapsules based solely on sodium alginate, those with a ratio of sanxan gum to sodium alginate of 4:6 demonstrated enhanced encapsulation efficiency (97.66 ± 0.79 %) and freeze-dried cell viability (9.97 ± 0.03 log CFU/g). Furthermore, the incorporation of chitosan yielded the highest encapsulation efficiency for the Lp @SG-SA@CS microcapsules (99.14 ± 1.46 %) and significantly enhanced their tolerance to heat, bile salts, and gastric acid. Our findings suggest that sanxan gum can confer acid resistance to microcapsules, highlighting its potential for applications in diverse acidic environments.
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Cuicui Duan,Yi Zhang,Hongru Wang,Siyang Sui,Hui Liu,Cheng Wu,Lin Ma,Fumin Ma,Xiaolei Li,Dan Li
β-LG-EPS-EGCG complex could effectively stabilize W 1 /O/W 2 emulsion during long-time storage. • W 1 /O/W 2 emulsion stabilized by β-LG-EPS-EGCG complex was used to encapsulate Lactobacillus plantarum. • Lactobacillus plantarum encapsulated in W 1 /O/W 2 emulsion was clearly observed under CLSM. • Flow cytometry was used to analyze the survival rate of strains under different treatments. The efficacy of probiotics is limited by their low survival capacity in human gastrointestinal tract. In this study, a water-in-oil-in-water (W 1 /O/W 2 ) emulsion delivery system stabilized by a ternary complex composed of β-lactoglobulin (β-LG), exopolysaccharide (EPS) and epigallocatechin gallate (EGCG) was constructed to encapsulate L. plantarum A81. Compared with native β-LG and binary β-LG-EPS complexes, the β-LG-EPS-EGCG ternary complex significantly decreased emulsion droplet size, improved interfacial protein adsorption efficiency, and formed a denser, elastic interfacial structure. Confocal laser scanning microscopy confirmed more uniform droplet distribution and favorable bacterial encapsulation in ternary complex-stabilized emulsions. Furthermore, the emulsion stabilized by β-LG-EPS-EGCG complex significantly improved the survival rate of strains during 30-day storage (from 27.62% ± 1.35% to 47.33% ± 1.23%), after pasteurization (from 10.55% ± 1.24% to 18.87% ± 1.56%), and after in vitro simulated gastrointestinal digestion (from 3.01% ± 0.81% to 8.97% ± 1.37%). These results demonstrate that β-LG-EPS-EGCG ternary complex is a good stabilizer for W1/O/W2 emulsion. Download: Download high-res image (295KB) Download: Download full-size image
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高影响因子
ANIMAL REPRODUCTION SCIENCE
·
2025
·
IF 3.3
Xi Huang,Zhangshan Gao,Peichao Gao,Helong Ji,Laizhi Xu,Dengfei Feng,Wentao Fan,Suquan Song
Stress is a well-established disruptor of reproductive function in poultry. Gamma-aminobutyric acid (GABA), a notable postbiotic derived from Lactobacillus plantarum ( L. plantarum ), has attracted increasing attention as a dietary supplement. While existing research predominantly focuses on GABA applications in broilers and other species, its effects on laying hens remain underexplored. This study investigated whether GABA and L. plantarum 1-2-3 supplementation mitigated corticosterone (CORT)-induced chronic stress in laying hens. Results demonstrated that both GABA and L. plantarum 1-2-3 effectively mitigated CORT-induced declines in laying performance and ovarian damage. They reduced ovarian oxidative stress, inflammation, and atretic follicles by enhancing antioxidant capacity and normalizing expression of inflammatory factors ( IL18 , LITAF , IL6 , IL1B) , and apoptosis-related factors ( BCL2 , BAX , CASP9 , CASP3 ). Furthermore, GABA and L. plantarum 1-2-3 markedly increased the serum reproductive hormone levels, as well as the transcription level of ovarian steroidogenic genes ( LHCGR, FSHR, StAR, CYP11A1 ), while modulated ovarian cholesterol metabolism via PPARα-mediated pathways. These findings elucidate the mechanisms by which GABA and L. plantarum 1-2-3 alleviate stress in laying hens, providing a theoretical and practical basis for their use as functional feed additives in stress-exposed flocks.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xinsheng Qin,Qingli Bo,Panzhu Qin,Sufang Wang,Kaiyong Liu
In this study, we demonstrated the role of whey protein isolate (WPI)/(−)-epigallocatechin-3-gallate (EGCG) covalent conjugates/gellan gum double network emulsion gels by duo-induction of glucono-δ-lactone (GDL) and CaCl 2 on the digestion viability of Lactobacillus Plantarum powders. We employed a novel one-step continuous cold gelation strategy to product double network emulsion gels. The gellan gum in continuous phase was first induced by CaCl 2 to form a gel in a short time. Besides, the GDL was improved covalent cross-linking between WPI-EGCG covalent conjugate Pickering particles or particle-coated oil droplets because of the slowly pH reduction. The first cross-linked gellan gum gels could be more closely integrated into the GDL-induced second gel networks with synergistic role, which promoted the formation of compact and homogeneous networks through hydrophobic interactions and disulfide bonds. Thus, the results provide a promising strategy for probiotic powders encapsulation of double network emulsion gels in oral applications of gel type foods.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Han Wu,Hao-Nan Liu,Ai-Min Ma,Jian-Zhong Zhou,Xiu-Dong Xia
In this study, Lactobacillus plantarum strains, namely 70810, B1-6, and ZR were used to confirm the synergetic effects of co-inoculation with Lactobacillus and Rhizopus oryzae on whole-oat solid state fermentation (SSF). The results indicated that number of viable bacterial cells increased more quickly (10.72–11.23-fold higher) during co-inoculated SSF than that during pure Lactobacillus fermentation. Fungal ergosterol content reached slightly less during co-inoculation (137.04–155.12 mg/kg) than that during R. oryzae fermentation (193.64 mg/kg). However, compared to R. oryzae , R. oryzae combined with Lactobacillus produced more obvious hydrolysis of macromolecules and accelerated more water transformation from confined to free form. As for co-inoculated SSF of oat, the reducing sugar content reached the highest at 41.09–47.42 g/100g and then dropped, and free amino acid nitrogen content varied gradually until 40.66–41.67 mg/100g. Fermented oats (FOs) had higher total phenolic content than unfermented oat (UO) (2.56–2.72-fold, FO vs . UO), and their DPPH radical scavenging activity, ferric reducing capacity, and hydroxyl scavenging activity were increased 2.04-, 2.10-, and 1.99-fold at maximum (FO vs . UO), respectively. The Lactobacillus showed strain dependence during co-inoculated SSF, among which B1-6 displayed the best to enhance oat's overall quality.
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高影响因子
Frontiers in Microbiology
·
2018
·
IF 4.019
Muhammad Zafarullah,Ramzan Rabia,Zhang Shanshan,Hu Haijuan,Hameed Ahsan,Bakry Amr M.,Dong Yongzhen,Wang Lufeng,Pan Siyi
Lead (Pb) is a well-recognized and potent heavy metal with non-biodegradable nature and can induce the oxidative stress, degenerative damages in tissues, and neural disorders. Certain lactic acid bacterial strains retain the potential to mitigate the lethal effects of Pb. The present work was carried out to assess the Pb bio-sorption and tolerance capabilities of Lactobacillus plantarum spp. Furthermore, potato resistant starch (PRS)-based microencapsulated and non-encapsulated L. plantarum KLDS 1.0344 was utilized for bioremediation against induced chronic Pb toxicity in mice. The experimental mice were divided into two main groups (Pb exposed and non-Pb exposed) and, each group was subsequently divided into three sub groups. The Pb exposed group was exposed to 100 mg/L Pb(NO3)2 via drinking water, and non-Pb exposed group was supplied with plain drinking water during 7 weeks prolonged in vivo study. The accumulation of Pb in blood, feces, renal, and hepatic tissues and its pathological damages were analyzed. The effect of Pb toxicity on the antioxidant enzyme capabilities in blood, serum, as well as, on levels of essential elements in tissues was also calculated. Moreover, KLDS 1.0344 displayed remarkable Pb binding capacity 72.34% and Pb tolerance (680 mg/L). Oral administration of both non- and PRS- encapsulated KLDS 1.0344 significantly provided protection against induced chronic Pb toxicity by increasing fecal Pb levels (445.65 ± 22.28 μg/g) and decreasing Pb in the blood up to 137.63 ± 2.43 μg/L, respectively. KLDS 1.0344 microencapsulated with PRS also relieved the renal and hepatic pathological damages and improved the antioxidant index by inhibiting changes in concentrations of glutathione peroxidase, glutathione, superoxide dismutase, malondialdehyde, and activated oxygen species, which were affected by the Pb exposure. Overall, our results suggested that L. plantarum KLDS 1.0344 either in free or encapsulated forms hold the potentiality to deliver a dietetic stratagem against Pb lethality.
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yejun Zhong,Hailin Zheng,Ruiyun Chen,Wei Liu,Shan Sun,Liqiang Zou,Er Sheng Gong
Dual-phase gelation in W/O/W emulsions enables robust, tunable probiotic delivery. • Oil-phase gelation using PGPR and wax reduces droplet size and raises viscosity. • Aqueous-phase gelation using CaCO₃-pectin/WPI network increases bound water. • Dual-phase gelation yields highest mechanical strength and probiotic survival. To overcome the poor stability and low probiotic survival associated with traditional delivery systems, this study systematically developed and compared water-in-oil-in-water (W/O/W) emulsions with different phase gelation strategies. Three emulsion types were constructed: oil-phase (O-W/O/W), external aqueous-phase (A-W/O/W), and dual-phase gelled (B-W/O/W) emulsions. The results revealed that dual-phase gelation (B-W/O/W) exhibited a superior synergistic effect compared to single-phase gelation. Both aqueous- and oil-phase gelation increased apparent viscosity and restricted internal water mobility, but their combination provided the most robust physical barrier. Specifically, oil-phase gelation reduced particle size by immobilizing free water, while aqueous gelation formed a dense protective network. This synergistic protection significantly enhanced probiotic viability during pasteurization, in vitro digestion, and long-term storage. This study demonstrates that dual-phase gelation is a highly effective and novel strategy for the development of high-performance delivery vehicles, providing a theoretical basis for the commercial application of sensitive probiotics in functional foods.
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高影响因子
Molecular Biomedicine
·
2026
·Vol. 7
·期 1
·129
·
IF 13
Wang Zhengshi,Jia Chengyou,Liu Yongqiang,Rixiati Youlutuziayi,Zhang Wentao,Mao Shiyu,Chen Haotian,Zou Libin,Ye Chen,Shen Bing,Yao Xudong
Prostate cancer (PCa) is one of the most common cancers in males, and its treatment remains challenging due to the tumor microenvironment (TME) with immunosuppressive properties and limited response to anti-PD-1 therapy. Gut microbiota-derived metabolites have recently emerged as modulators of cancer immunometabolism, however, their role in PCa progression and immunotherapy is poorly understood. Here we found that indole-3-lactic acid (ILA), a metabolite produced by Lactobacillus plantarum , exerted dual anti-tumor effects on PCa cells and the TME. Mechanistically, ILA activates the aryl hydrocarbon receptor (AHR), and the resulting AHR/ARNT heterodimer translocates into the nucleus and binds to the promoter of ASF1B. This heterodimer then recruits the HDAC1/2-NuRD complex to reduce H3K27ac levels and suppress ASF1B expression. ASF1B binds to specific residues of ENO1 via its N-terminal core domain and enhances ENO1 enzymatic activity. ILA-induced downregulation of ASF1B impairs this interaction, reduces ENO1 activity, and suppresses the PI3K/Akt pathway, thereby inhibiting the malignant phenotypes of PCa cells. Concurrently, ILA decreased CXCL8 secretion by inhibiting the PI3K/Akt/NF-κB pathway, enhancing CD8 + T cell infiltration and M1 macrophage polarization, thereby remodeling the TME. Additionally, ILA synergized with anti-PD-1 therapy to more effectively suppress tumor growth. These findings reveal a novel mechanism by which gut microbiota-derived metabolites regulate PCa progression and immunometabolism, positioning ILA as a potential therapeutic agent to improve PCa treatment.
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高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Xin-Sheng Qin,Qun-Yu Gao,Zhi-Gang Luo
In this study, we demonstrated the role of Pickering high internal phase emulsions (HIPEs) stabilized with the whey protein isolate (WPI)/(-)-epigallocatechin-3-gallate (EGCG) covalent conjugates on the storage and GIT passage viability of probiotics powder ( Lactobacillus Plantarum ). At pH 9 value, the WPI-EGCG covalent conjugates by free-radical induction reaction formed hydroxyquinone or hydroxyquinone dimers with better wettability, and digestive enzyme inhibition effect and smaller particle size. Then, the WPI-EGCG covalent conjugates were used to stabilize the O/W type Pickering emulsion. The oil fraction ( ϕ ) addition led to an increase in the droplet size and modulus of Pickering emulsions toward to generate gel-like structure. On the other hand, at a constant ϕ value, the droplet size of these Pickering HIPEs reduced with increasing particle concentration ( c ) from 0.5% to 5%, indicating that high c can stabilize more oil droplets. Encapsulation of Lactobacillus Plantarum powder with the Pickering HIPEs was successful to enhance the viable cell count after 14 days storage and GIT digestion. The protective effect of Pickering emulsions was improved with the addition of the c and/or ϕ value. Therefore, Pickering HIPEs can be applied as a promising carrier for enhancing the viability of probiotic powder during storage and GIT digestion by reason of the high oil phase fraction and the adsorption of the WPI-EGCG covalent conjugates on the droplet interface.
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高影响因子
JOURNAL OF AGRICULTURAL SCIENCE
·
2024
·Vol. 161
·期 6
·877-886
·
IF 1.7
Xinbao Li,Haopeng Liu,Tongtong Dai,Dong Dong,Guofeng Xu,Yushan Jia,Tao Shao
The work aimed to evaluate the effect of lactic acid bacteria, natamycin and hexanoic acid on the fermentation quality, aerobic stability and in vitro digestibility of total mixed ration (TMR) silages containing water bamboo sheath leaves. The treatments were as follows: (1) no additives (control, C), (2) lactic acid bacteria (L), (3) natamycin (N), (4) hexanoic acid (H), (5) lactic acid bacteria + natamycin (LN), (6) lactic acid bacteria + hexanoic acid (LH). Silos were opened and silages were evaluated to assess fermentation quality, aerobic stability and in vitro digestibility. All TMR silages were well-preserved as indicated by dominant lactic acid content, low ammonia nitrogen/total nitrogen, trace propionic acid and negligible butyric acid contents. All additives improved the fermentation quality and in vitro digestibility, evidenced by higher lactic acid/acetic acid ratio, lactic acid, acetic acid, water-soluble carbohydrate contents and lactic acid bacteria count, and lower pH, aerobic bacteria and yeast counts. The LN had the highest lactic acid, acetic acid, lactic acid/acetic acid ratio, in vitro gas production and in vitro digestibility. During aerobic exposure, the LN silage had the highest lactic acid and acetic acid contents, and the lowest pH among all TMR silages. The LN and LH prolonged aerobic stability, reaching 85.5 and 81.5 h, respectively. The LN is recommended to improve the fermentation quality, aerobic stability and in vitro digestibility of TMR prepared with water bamboo sheath leaves.
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Food & Function
·
2024
·
IF 5.1
Zhaojuan Shi,Jun Wu,Xingting Wang,Tongqiao Nie,Qingmei Zeng,Chuanxun Yuan,Risheng Jin
Probiotics have considerable interest due to their inseparable link to human health. However, probiotic products are seriously challenged during processing, preservation, and intake. Food-grade probiotic delivery systems need to be further explored as an effective way to enhance cell viability. In this study, water-in-oil (W/O) Pickering emulsions were fabricated by adding candelilla wax (CLW) as a network stabilizer based on acylated EGCG derivatives in the crystalline form as a Pickering stabilizer. The effects of acylated EGCG derivatives’ concentration, CLW concentration, and oil phase volume fraction on the droplet size distribution, microstructure, and physical stability of Pickering emulsions were explored. The presence of CLW reduced the particle size and improved the physical stability of acylated EGCG-based emulsions, and the effect was more positive with increasing concentration. The protective effect of emulsions with different oil phase volume fractions on Lactobacillus plantarum during freeze-thaw cycles, storage, and gastrointestinal digestion was evaluated. The outer-phase physical barrier of W/O emulsions co-stabilized with acylated EGCG derivatives and CLW facilitated the sensitivity of probiotics to ice crystal growth, temperature changes, acidic environments, and digestive enzymes. The emulsions formulated with 40% oil phase volume fractions allowed Lactobacillus plantarum to survive up to 7.75 log CFU g−1 in the harsh gastrointestinal environment. The results offer promising strategies for applying W/O emulsion probiotic delivery systems in food processing, storage, and oral administration.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yingjie Zhou,Lijie Zhu,Yingyan Li,Feng Guo,Lei Chen,Guozhen Wang,Qian Shen,Xiuying Liu,Wenping Ding
In this study, soyasaponin (Ssa), corn oil, and soybean protein isolate (SPI) were used to prepare a water-in-oil-in-water (W/O/W) double emulsion system that can protect Lactobacillus plantarum (L.P) in the upper gastrointestinal tract for delivery to the small intestine and colon. Incorporating different amounts of L.P (0.1–0.6% (w/v)) in the W/O/W double emulsion caused the viscoelastic modulus and droplet size of the L.P-embedded emulsion system to change, and the emulsion system transformed from a non-Newtonian fluid to a “solid-like” gel. These results revealed that L.P encapsulation led to significantly improved stability of the W/O/W emulsion. In addition, different concentrations of L.P had different effects on the survival rate of L.P in simulated digestive fluids, and after in vitro digestion, L.P was naturally delivered from the stomach into the intestines. The optimal concentration of L.P for obtaining a stable emulsion was found to be 0.3%, and the survival rate of the encapsulated L.P in the small intestine was as high as 78.96% after 1 h. Thus, choosing an appropriate amount of L.P was crucial. The W/O/W emulsion system effectively maintained the viability of L.P and successfully delivered it to the small intestine and colon.
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高影响因子
危险品化学品经营许可证（带存储）