FOOD CHEMISTRY
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2024
·
IF 8.5
Xin Zhou,Jie Shi,Na Yu,Xiuzhen Zhu,Qiqi Zhang,Lanlan Ma,Shan Mao,Wenbao Zuo,Xia Zhang,Jianhong Yang
Pickering emulsion gels received extensive attention in encapsulating fat-soluble substances such as Lycium barbarum seed oil (LBSO). However, the gels presented poor mechanical properties, otherwise, their physical encapsulation cannot inhibit lipid peroxidation. Herein, grape seed proanthocyanidins (OPCs) and casein (CAS) complexes interacted through hydrogen and covalent bonds were proposed to build Pickering emulsion gels and encapsulate LBSO, which changed the secondary structures of CAS and further enhanced emulsifying ability, oxidation resistance, and gelling performance. The CAS-OPCs gels had better microstructures and mechanical properties due to the enhancement of hydrogen and covalent interactions. Furthermore, gels with OPC contents of 8.00 mg/mL had performance in 3D printing. And gels reduced the peroxide value of LBSO (9.33 ± 0.20 to 1.39 ± 0.22 mmol/kg) after heating. This study helps reveal the possible mechanisms of OPCs on gels and provides a reference for the application and research of OPCs and CAS composites in Pickering emulsion gels.
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高影响因子
FOOD CHEMISTRY
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2025
·
IF 9.8
Lisi Liang,Lianzhu Lin,Mouming Zhao
Lycium barbarum polysaccharide (LBP) is a prebiotic that promotes the proliferation of beneficial bacteria, but lacks of regulatory function on harmful bacteria. In this study, chlorogenic acid (CGA) was used to achieve the functional enhancement of two LBPs (LBP-A and LBP-M). The combination of CGA resulted in changes in the solution properties of LBPs, manifested as increased pseudoplasticity, viscosity, turbidity, and decreased water mobility, absolute potential value, pH value. CGA was non-covalently bound to LBPs through hydrophobic interaction and hydrogen bond. LBP-A had strong affinity with CGA, mainly through hydrophobic interaction, while LBP-M had weak affinity with CGA, mainly through hydrogen bond. Both LBP-CGA complexes exhibited Bacteroides proliferative activity and gut pathogens inhibitory activity, among which LBP-A-CGA complex showed strong inhibitory effects on Escherichia coli and Bacillus cereus , and LBP-M-CGA complex showed strong inhibitory effect on Staphylococcus aureus . This study provided guidance for the development of LBP-CGA as novel prebiotic.
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Toxics
·
2025
·Vol. 13
·期 8
·677
·
IF 4.1
Zhen Zhang,Yitong Shang,Hong Yang,Liyang Ding,Yu Deng,Bo Xu,Xufeng Fu
Male infertility, as a globally significant reproductive health issue, remains idiopathic in over 40% of cases. Reproductive disorders in males induced by environmental pollutants, such as di(2-ethylhexyl) phthalate (DEHP), have garnered considerable attention in recent years. DEHP induces testicular oxidative stress and ferroptosis via its active metabolite MEHP, thereby leading to spermatogenic dysfunction.Lycium barbarumpolysaccharide (LBP), a traditional food and medicine homologous substance, exhibits potential antioxidant and reproductive protective properties. However, the underlying mechanism by which LBP intervenes in the toxicity induced by DEHP remains to be elucidated. This study explored the protective effect and molecular mechanism of LBP on DEHP-induced testicular injury through in vivo and in vitro experiments. The result showed that DEHP exposure (150 mg/L in free drinking water for 6 weeks) significantly decreased testicular weight, sperm concentration, and sperm motility in mice, while DEHP exposure induced pathological damage to testicular tissue, as evidenced by cavitation of seminiferous tubules, reduced numbers of spermatocytes, and vacuolar degeneration of Sertoli cells. However, LBP (450 mg/L) treatment significantly reversed testicular damage and sperm parameters. In vitro, MEHP reduced the viability of GC2 cells (spermatocyte cell line) and TM4 cells (Sertoli cell line), and LBP significantly restored cell activity. Mechanistically, exposure to DEHP/MEHP results in iron overload (elevated levels of free Fe2+), lipid peroxidation (increased MDA and reduced GSH), and dysregulated expression of key proteins involved in ferroptosis and iron homeostasis within the testis and cells. Furthermore, it was demonstrated that when NRF2 was specifically inhibited by ML385 or silenced via siRNA, the protective effects of LBP were abrogated, thereby validating the critical role of NRF2 in the regulation of iron homeostasis by LBP. In conclusion, LBP mitigates DEHP-induced testicular injury by activating NRF2 to regulate iron homeostasis in Sertoli cells and spermatocytes cells. This study not only offers a potential strategy for the prevention and treatment of male reproductive disorders caused by DEHP exposure, but also underscores the reproductive protective effects and application prospects of LBP in this context.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hong Yang,Liyang Ding,Bo Xu,Zhen Zhang,Wenjie Dai,Tiantian He,Ling Liu,Xing Du,Xufeng Fu
Di-(2-ethylhexyl)phthalate (DEHP) is a common plasticizer that has been shown to significantly negatively affect male reproductive health. On the other hand, Lycium barbarum polysaccharide (LBP) has been shown to improve reproductive function. Therefore, we hypothesized that LBP may ameliorate DEHP-induced male reproductive damage. Herein, we found that LBP could alleviate DEHP-induced testicular damage and sperm abnormalities. Furthermore, histomorphological analysis of mice testis revealed that LBP primarily ameliorated the DEHP-induced male reproductive damage by targeting Sertoli cells. Moreover, the detection of the function-related genes of Sertoli cells confirmed this finding. The serum of mice in the Control, DEHP, and DEHP+LBP groups was analyzed using non-targeted metabolomics to further elucidate the mechanism of action of LBP in improving DEHP-induced male reproductive damage. According to the results, the differential metabolites were mainly enriched in the glutamate metabolism pathway, implying that LBP may alleviate the ferroptosis-related DEHP-induced testicular injury. Related ferroptosis markers were also found in mice testis. These findings collectively suggest that LBP may ameliorate DEHP-induced testicular injury via alleviating ferroptosis in Sertoli cells. To clarify the specific mechanism, we constructed a cell model in vitro by treating TM4 cells (the Sertoli cell line) with LBP and MEHP (the in vivo DEHP metabolite). Our findings revealed that LBP can improve the function of DEHP-affected Sertoli cells. Furthermore, the analysis of lipid peroxidation, Fe 2+ content, and ferroptosis-related protein expressions demonstrated that LBP could ameliorate MEHP-induced ferroptosis in TM4 cells. To clarify the specific mechanism, glutamate metabolism-related proteins involved in the ferroptosis pathway were detected. According to the results, there were significant changes in the expression of NRF2, SLC7A11 and GPX4 proteins, which are involved in the ferroptosis glutamate metabolism pathway. Furthermore, supplementation of NRF2, SLC7A11, and GPX4 inhibitors (ML385, Erastin, and RSL3, respectively) blocked the therapeutic effect of LBP in alleviating MEHP-induced ferroptosis in TM4 cells, implying that LBP could also ameliorate MEHP-induced ferroptosis via the NRF2/SLC7A11/GPX4 pathway. In summary, these findings show that LBP can alleviate DEHP/MEHP-induced ferroptosis through the NRF2/SLC7A11/GPX4 pathway, ameliorating Sertoli cell dysfunction and improving the DEHP-induced male reproductive damage. Therefore, the clinical administration of LBP could be an effective strategy for preventing DEHP-induced male reproductive injury.
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Current Research in Food Science
·
2025
·
IF 7
Meilin Cui,Keke Qiu,Wei Zhao,Jiali Wang,Xiuhong Zhang
Brown rice has great nutritional values and gradually becomes an excellent health-focus staple to consumers. While it seriously suffers from starch retrogradation, resulting in poor textural quality and limited shelf life. This study investigated the effects of combined treatment of dielectric barrier discharge (DBD) and Lycium barbarum peptides (LBP) on the structural and physicochemical properties of brown rice starch (BRS). The results revealed that DBD, LBP, or their combined treatments significantly affected the structural features of BRS, including a decrease in the amylose content, relative crystallinity, molecular weight distribution of amylose and amylopectin, short-range ordering, and the ratio of double-helix structure; while the proportion of amorphous structure increased. We also found that amylopectin branch chain length distribution shifted to shorter chains (A and B1 chains), especially in DBD- and combined-treated samples. Moreover, these modifications reduced the gel structure strength, pasting viscosity, and disrupted water-binding capacity shifting to more free water, drastically retarding the retrogradation behaviors of BRS. Significantly, dual modification outperformed individual treatments and showed more noticeable and functional properties. This enhancement could be ascribed to the depolymerization and etching effects of DBD treatment improved the accessibility for hydrogen bond interactions between LBP and BRS, highlighting a green strategy to fundamentally inhibit BRS retrogradation. The combination of DBD and LBP offers a promising approach for designing stable, high-quality starchy foods with extended shelf-life and advances the application of physical-biological combinatorial starch modification.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Jinghong Wei,Wangting Zhou,Wei Yi,Jiameng Shi,Yamei Yan,Jia Mi,Lu Lu,Youlong Cao,Xiaoxiong Zeng
Polysaccharides from the fruits of Lycium barbarum L. (LBPs) are the principal active constituents of L. barbarum , and it has been reported that LBPs exert regulatory effects on the gut microbiota, particularly in promoting the growth of Bacteroides . In this study, the in vitro anaerobic fermentation revealed that LBPs-4, a purified fraction of the crude LBPs, increased the relative abundances of 11 Bacteroides species, particularly B. ovatus . Results of the pure cultures indicated B. ovatus , B. thetaiotaomicron , B. fragilis and B. uniformis could effectively utilize LBPs-4 for their own growth. Transcriptome analysis of B. ovatus indicated that the degradation of LBPs-4 involved the up-regulation of three polysaccharide utilization loci (PULs). Among them, Bovatus _00022 in PUL1, which belongs to the GH28 family and is annotated as a polygalacturonase, is one of the major carbohydrate-active enzymes in the metabolism of LBPs-4. BO PG00022 was preliminarily confirmed to be effective in the degradation via the hydrolysis analysis. In conclusion, this study provided significant insights into the degradation mechanism of LBPs-4 employing gut microflora or enzymes as targets, providing a theoretical basis for the precise utilization and structural verification of complex glycans.
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Foods
·
2022
·Vol. 11
·期 22
·3731
·
IF 5.561
Ziying Hu,Yanan Ma,Jun Liu,Yijun Fan,Anran Zheng,Pengyan Gao,Liang Wang,Dunhua Liu
Goji berry (Lycium barbarumL., LBL) is a good source of carotenoids, while the bioaccessibility of various types of LBL carotenoids has not been explored. In the study, eight carotenoids, three carotenoid esters and two carotenoid glycosylated derivatives were identified by a non−targeted metabolomics approach. The dried LBL (DRI), LBL in water (WAT), and LBL in “Baijiu” (WIN) were used to recreate the three regularly chosen types of utilization, and the in vitro digestion model showed that the bioaccessibility of the carotenoids increased significantly from the oral to the gastric and intestinal phase (p< 0.05). The bioaccessibility of LBL carotenoids was the most elevated for DRI (at 28.2%), followed by WIN and WAT (at 24.9% and 20.3%, respectively). Among the three carotenoids, zeaxanthin dipalmitate showed the highest bioaccessibility (51.8–57.1%), followed by β−carotene (51.1–55.6%) and zeaxanthin (45.2–56.3%). However, the zeaxanthin from DRI exhibited significantly higher bioaccessibility (up to 58.3%) than WAT and WIN in both the gastric and intestinal phases (p< 0.05). Results of antioxidant activity tests based on DPPH, FRAP, and ABTS showed that the addition of lipids improved the bioaccessibility of the carotenoids. (p< 0.05).Keywords:goji berry;bioaccessibility;carotenoids;metabolomics;zeaxanthin dipalmita
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Food Chemistry-X
·
2026
·
IF 9.2
Ying Liu,Linlin Yu,Shuxiang Yan,Xiaowei Fang,Tenggao Zhu,Huanwen Chen,Dong Wu
Metabolic changes during the ripening of wolfberry ( Lycium barbarum L.) fruits reflect their quality and pharmacological properties. To decipher the metabolic evolution underlying this physiological process, a systematic analysis of wolfberry fruits at different ripening stages (7–35 days after anthesis) was conducted by theta-nano-extractive electrospray ionization mass spectrometry. Multivariate analysis effectively discriminated the various stages and identified proline, γ-aminobutyric acid, arginine, and N 1 -caffeoyl- N 10 -dihydrocaffeoyl spermidine as potential differential metabolites. Polyamine and flavonoid metabolism were significantly activated during the early ripening stages, leading to the rapid accumulation of antioxidant components such as spermidine, rutin, and chlorogenic acid. In the late ripening stages, amino acid metabolism was markedly enhanced, resulting in a substantial increase in betaine, serine, and threonine. This study elucidates the key stages and metabolic foundation of quality formation in wolfberry fruits at the metabolomic level, providing a theoretical basis for determining the optimal harvest time and regulating nutritional quality.
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Food Science & Nutrition
·
2026
·
IF 5
Shuzhen Fang,Kangyi Zhang,Xiang Fang,Danqing Liu,Yulong Yang,Guijie Chen,Wenming Yang
Wilson's disease (WD) is a rare autosomal recessive disorder characterized by impaired copper metabolism and progressive multi‐organ damage. Emerging evidence suggests that gut microbiota dysbiosis and reduced microbial fermentation capacity may contribute to the progression of liver diseases. However, whether the gut microbiota can serve as a therapeutic target in WD remains unclear. This study aimed to investigate the modulatory effects of Lycium barbarum polysaccharides (LBPs), a bioactive component of the traditional Chinese medicine Gan‐Dou‐Fu‐Mu decoction, on gut microbiota composition and short‐chain fatty acids (SCFAs) production in WD, and to explore their potential as microbiota‐targeted prebiotic interventions. WD‐associated microbiota exhibited decreased microbial diversity and significantly lower SCFAs production, particularly acetic acid and total SCFAs, compared to healthy controls. LBPs supplementation significantly increased the relative abundances of beneficial genera, including Lactobacillus , Lacticaseibacillus , and Paraeggerthella , as well as SCFAs‐producing species such as Bacteroides fragilis and Lactobacillus delbrueckii . Notably, LBPs markedly enhanced acetic acid and total SCFA concentrations in WD microbiota, outperforming the standard treatment agent penicillamine. These findings highlight the promising prebiotic potential of LBPs and support their application as a novel microbiota‐targeted adjunctive therapy for WD. Schematic overview of the experimental workflow investigating the effects of LBPs on gut microbiota.
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Agriculture-Basel
·
2025
·Vol. 16
·期 1
·83
·
IF 3.6
Yunfei Zhang,Xinyi Hu,Junjie Yin,Jiabin Chen,Shujing Zhang,Fang Wang
Chinese wolfberry (Lycium barbarumL.), a specialty crop with ecological, medical, and economic value in Ningxia province of China, is subject to severe damage fromAphis gossypiiGlover. Currently,A. gossypiipopulations show extremely high-level resistance to beta-cypermethrin in the major wolfberry planting areas in Ningxia. The specific resistance mechanisms, however, are still not known. In this work, we collected a fieldA. gossypiistrain (HSP) from a wolfberry orchard in Ningxia in 2021 using a single-time sampling method, and its resistance to beta-cypermethrin was determined to be extremely high (994.74-fold) as compared with that of a susceptible strain (SS). Then we explored the potential resistance mechanisms from two aspects, namely, metabolic detoxification and target-site alterations. Bioassays of beta-cypermethrin with or without a synergist showed that piperonyl butoxide (PBO) significantly increased the toxicity of beta-cypermethrin (4.72-fold) to the HSP strain, while triphenyl phosphate (TPP) and diethyl maleate (DEM) exhibited no significant synergistic effects. Correspondingly, theO-demethylase activity of cytochrome P450s in the HSP strain was 1.68-fold higher than that in the susceptive strain (SS), whereas changes in carboxylesterases and glutathioneS-transferases activities were unremarkable. Also, fifteen upregulated P450 genes were identified by both RNA-Seq and qRT-PCR technologies, containing eleven CYP6 genes, three CYP4 genes, and one CYP380 gene. Especially, five CYP6 genes with high relative expression levels (>3.00-fold) were intensively expressed by beta-cypermethrin induction in the HSP aphids. These metabolism-related results indicate the key role of P450-mediated metabolic detoxification in HSP resistance to beta-cypermethrin. Sequencing of voltage-gated sodium channel (VGSC) genes identified a prevalent M918L mutation and a new G1012D mutation in HSPA. gossypii. Moreover, heterozygous 918 M/L and 918 M/L + G1012D mutations were the dominant genotypes with frequencies of 60.00% and 36.67% in the HSP population, respectively. Overall,VGSCmutations along with P450-mediated metabolic resistance contributed to the extremely high resistance of the HSP wolfberry aphids to beta-cypermethrin, providing support forA. gossypiicontrol and resistance management in the wolfberry planting areas of Ningxia using insecticides with different modes of action.
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Frontiers in Pharmacology
·
2026
·
IF 5.4
Shuai Zhao,Zhenyao Tan,Jiaxin Suo,Yang Bu
Introduction Colorectal cancer liver metastasis (CRLM) is the leading cause of death in colorectal cancer, and nonalcoholic fatty liver disease (NAFLD) promotes CRLM. Lycium barbarum polysaccharides (LBPs), bioactive metabolites of the traditional medicinal plant Lycium barbarum L, inhibit the progression of colorectal cancer and NAFLD by regulating gut microbiota composition. However, their roles in preventing CRLM under NAFLD conditions remain unclear. This study aimed to investigate the preventive effect of LBPs on liver metastasis of colorectal cancer in the context of NAFLD and explore its potential mechanisms. Methods An NAFLD mouse model was established, followed by prophylactic oral administration of LBPs by gavage for 28 days before splenic injection of MC38 colorectal cancer cells to establish liver metastasis. Pseudo-germ-free mice combined with fecal microbiota transplantation were constructed to explore the role of the gut microbiota in the preventive effect of LBPs on CRLM. Gut microbiota and fecal short-chain fatty acids were analyzed by 16S rRNA sequencing and liquid chromatography–mass spectrometry. Spearman’s correlation analysis was used to explore the correlation between bacterial genera and liver lipid metabolism indicators. Serum non-targeted metabolomic profiling and transcriptomic analysis of CRLM cells were performed to elucidate metabolic and molecular mechanisms. Results Under NAFLD conditions, LBPs markedly reduced hepatic metastatic burden, liver weight, and liver-to-body weight ratio. LBPs ameliorated hepatic lipid metabolism and restored colonic barrier integrity in NAFLD mice. The gut microbiota was identified as a critical mediator of LBPs-induced protection against CRLM, and depletion of the microbiota completely abrogated the anti-metastatic effects of LBPs. LBPs enhanced microbial diversity and richness, enriched of short-chain fatty acid-producing bacterial genera, such as Cryptobacteroides, Evtepia, and Bacteroides -H, and elevated colonic butyrate levels. Metabolomic profiling revealed reduced serum acylcarnitines and increased organic acids. Transcriptomic profiling showed upregulation of fibroblast growth factor 21, activation of the PI3K-AKT signaling pathway, and promotion of epithelial–mesenchymal transition in colorectal cancer cells, while LBPs reverse these changes. Discussion LBPs prevent CRLM associated with NAFLD by modulating the gut microbiota, enhancing butyrate production, improving hepatic metabolic homeostasis, and suppressing prometastatic signaling pathways. These findings highlight LBPs as promising preventive agents against CRLM in the setting of metabolic liver disease.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2026
·
IF 5.2
Xiumei Liu,Zi Yan,Jing Li,Hong Zou,Haoyue Zhong,Peixin Lee,Yaqi Lyu,Nianping Feng
First study of LB-Exos in modulating ocular disease. • LB-Exos present as natural, multi-target nanotherapeutics for diabetic retinopathy. • LB-Exos protect against oxidative stress, restore blood-retinal barrier integrity. • LB-Exos downregulate the HIF-1α/VEGF/VEGFR2 axis. • LB-Exos exhibit synergistic effect with standard anti-VEGF therapy. The growing interest in plant-derived extracellular vesicles (EVs) as nanotherapeutics has positioned Lycium barbarum L. EVs (LB-Exos) as a promising candidate for treating diabetic retinopathy (DR), a condition characterized by pathological angiogenesis and blood-retinal barrier (BRB) dysfunction. This study investigates the multifaceted therapeutic effects of LB-Exos, isolated from fresh goji berries and characterized for their bioactive metabolite cargo. In vitro, LB-Exos protected ARPE-19 cells from apoptosis by modulating the MAPK pathway and the Bcl-2/BAX/caspase-3 axis. In HUVECs, LB-Exos suppressed angiogenesis by downregulating the HIF-1α/VEGF/VEGFR2 axis and ANGPT2, showing synergistic effects with the standard therapy of bevacizumab (BVZ). These mechanisms were validated in a streptozotocin-induced diabetic mouse model, where LB-Exos treatment alleviated retinal pathology, reduced neovascularization, and restored BRB integrity by upregulating tight junction proteins. Our study establishes LB-Exos as a novel, natural nanoplatform that concurrently targets multiple pathological pathways in DR. This work elucidates the multifaceted therapeutic potential of PDEVs in ophthalmology and highlights their complementary mechanism to enhance current anti-VEGF therapy. Download: Download high-res image (321KB) Download: Download full-size image
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