PHARMACOLOGICAL RESEARCH
·
2020
·
IF 5.893
Ruocong Yang,Hao Liu,Chen Bai,Yingchao Wang,Xiaohui Zhang,Rui Guo,Siying Wu,Jianxun Wang,Elaine Leung,Hang Chang,Peng Li,Tiegang Liu,Yi Wang
The Coronavirus Disease 2019 (COVID-19) pandemic has become a huge threaten to global health, which raise urgent demand of developing efficient therapeutic strategy. The aim of the present study is to dissect the chemical composition and the pharmacological mechanism of Qingfei Paidu Decoction (QFPD), a clinically used Chinese medicine for treating COVID-19 patients in China. Through comprehensive analysis by liquid chromatography coupled with high resolution mass spectrometry (MS), a total of 129 compounds of QFPD were putatively identified. We also constructed molecular networking of mass spectrometry data to classify these compounds into 14 main clusters, in which exhibited specific patterns of flavonoids (45 %), glycosides (15 %), carboxylic acids (10 %), and saponins (5 %). The target network model of QFPD, established by predicting and collecting the targets of identified compounds, indicated a pivotal role of Ma Xing Shi Gan Decoction (MXSG) in the therapeutic efficacy of QFPD. Supportively, through transcriptomic analysis of gene expression after MXSG administration in rat model of LPS-induced pneumonia, the thrombin and Toll-like receptor (TLR) signaling pathway were suggested to be essential pathways for MXSG mediated anti-inflammatory effects. Besides, changes in content of major compounds in MXSG during decoction were found by the chemical analysis. We also validate that one major compound in MXSG, i.e. glycyrrhizic acid, inhibited TLR agonists induced IL-6 production in macrophage. In conclusion, the integration of in silico and experimental results indicated that the therapeutic effects of QFPD against COVID-19 may be attributed to the anti-inflammatory effects of MXSG, which supports the rationality of the compatibility of TCM.
研究领域:
药理学
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医学
·
中医药
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生物信息学
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分子生物学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Hao Tian,Hongpei Deng,Xinlong Liu,Chang Liu,Chuan Zhang,Kam W. Leong,Jing Ruan,Xianqun Fan
Uveal melanoma (UM) is the most prevalent primary ocular malignancy in adults, with high lethality and limited effective treatment options. Despite identified driver mutations in GNAQ , GNA11 , and BAP1 , therapeutic advancements have been minimal. This study highlights the pivotal role of N6-methyladenosine (m 6 A) modifications in UM pathogenesis and progression, focusing on the demethylase FTO as a therapeutic target. Elevated FTO expression in UM tissues correlates with decreased m 6 A levels, increased aggressiveness, and poor prognosis. The FTO inhibitor meclofenamic acid (MA) restored m 6 A levels, upregulated SLC7A11, and induced disulfidptosis, a unique form of cell death triggered by GSH depletion and NADPH consumption. To address MA's limitations in bioavailability and tumor targeting, we developed an MA-loaded nucleic acid nanodrug (SNAMA). SNAMA demonstrated effective tumor growth inhibition in orthotopic and metastatic UM models through GSH-responsive release and m 6 A-mediated disulfidptosis activation. Incorporating a PD-L1 aptamer into SNAMA further improved tumor targeting and immune modulation, enhancing therapeutic efficacy. This study identifies FTO as a critical target for UM therapy and introduces SNAMA-apt as a promising nanodrug. The findings offer a foundation for m 6 A-targeted approaches in UM and other malignancies, addressing bioavailability, targeting, and immune evasion challenges.
研究领域:
肿瘤学
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分子生物学
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药理学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2023
·
IF 5
Tang Xinjing,Wang Zhirong,Zheng Jiong,Kan Jianquan,Chen Guangjing,Du Muying
To enhance the content of adlay bran soluble dietary fiber (SDF) and improve its functionality, we investigated the influences of steam explosion (SE) on the physicochemical, structural properties, and in vitro hypoglycemic activities of adlay bran SDF. The cellulose, hemicellulose, and lignin contents of adlay bran decreased significantly after SE treatment. When the SE strength was 0.8 MPa for 3 min, the SDF content was 9.37%, which was a significant increase of 27.48% compared to the control. Under these conditions, SDF showed the highest oil-holding capacity (OHC) (2.18 g/g), cholesterol adsorption capacity (CAC) (27.29 mg/g), glucose adsorption capacity (GAC) (15.54 mg/g), glucose dialysis retardation index (GDRI) (36.57%), and α-Amylase activity inhibition ratio (α-AAIR) (74.14%). Compared with SDF from untreated adlay bran, SDF from SE-treated adlay bran showed lower weight molecular. In addition, differential scanning calorimetry (DSC) measurement showed that the peak temperature of SDF from adlay bran treated by SE increased by 4.19°C compared to the untreated SDF sample. The structure of SDF from adlay bran treated by SE showed that the SDF surface was rough and poriferous and the specific surface areas increased. In conclusion, SE pretreatment increases the content of SDF in adlay bran and improves its physicochemical, structural properties, and biological activities, which will be beneficial for the further exploitation of adlay bran.
研究领域:
食品科学
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生物技术
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营养学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Chasina Wang,Fengqing Jiao,Liyuan Zhang,Yishi Wu,Chuanlang Zhan
One of the key issues of lead-free halide double perovskite nanocrystals (DPNCs) is their low photoluminescence quantum yields (PLQYs), and the PLQYs of nondoped pristine DPNCs are currently below 2%, though they can be enhanced via strategies such as ion doping or surface passivation. Herein, we report an organic–inorganic hybrid approach to significantly increase the PLQYs of nondoped pristine DPNCs. With Cs2NaBiCl6 NCs as the model, we selected methylammonium (MA+) and formamidine (FA+) as organic A-site ions and for the first time successfully synthesized the organic–inorganic hybrid in-direct bandgap DPNCs, e.g., MA2NaBiCl6 and FA2NaBiCl6. The PLQYs of the prepared Cs2NaBiCl6, MA2NaBiCl6, and FA2NaBiCl6 are 1.9%, 6.2%, and 15.4%, respectively. The enhancement of PLQYs can be due to the lattice distortion on using the unsymmetric rod-like MA+ and V-like FA+ to replace the symmetric Cs+, which yields faster charge transfer, enhanced excitation, and reduced nonradiative recombination, which are combined to make the self-trapped excited state brighter with significantly enhanced PLQYs. Overall, our results clearly demonstrate that the organic–inorganic hybrid strategy is an effective way to tune the lattice distortion and significantly enhance the emission of nondoped DPNCs, which will open doors for designing functional optoelectronic materials.
研究领域:
材料科学
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纳米技术
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光电子学
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无机化学
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无机-有机杂化材料
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半导体纳米晶体
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光物理
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Caiying Zhang,Zhisheng Hu,Ruiming Hu,Shaoxing Pi,Zejing Wei,Chang Wang,Fan Yang,Chenghong Xing,Gaohui Nie,Guoliang Hu
Pyroptosis and autophagy are two different biological processes that determine cell fates. Our previous studies revealed that pyroptosis and autophagy were involved in cytotoxicity co-induced by molybdenum (Mo) and cadmium (Cd) in duck renal tubular epithelial cells, but crosstalk between them is unclear. Hence, the cells were treated with 500.0 μM Mo, 4.0 μM Cd, 10.0 μM Z-YVAD-fluoromethylketone (YVAD), 2.5 μM 3-methyladenine (3-MA) and 10.0 μM chloroquine (CQ) alone or in combination for 12 h (CQ for the last 4 h). Under Mo and Cd co-stress, data evidenced that YVAD addition decreased the number of autophagosomes, LC3 puncta, and AMPKα-1, Atg5, Beclin-1, LC3A, LC3B mRNA levels and LC3-II/LC3-I, Beclin-1 protein levels, and increased p62 expression levels. Besides, both 3-MA and CQ addition increased NLRP3, Caspase-1, NEK7, ASC, GSDMA, GSDME, IL-1β, IL-18 mRNA levels, NLRP3, Caspase-1 p20, ASC, GSDMD protein and ROS levels, and NO, LDH, IL-1β, IL-18 releases. Collectively, our results revealed that pyroptosis and autophagy co-induced by Mo and Cd were interrelated in duck renal tubular epithelial cells, and inhibiting pyroptosis might attenuate Mo and Cd co-induced autophagy, but inhibiting autophagy might promote Mo and Cd co-induced pyroptosis.
研究领域:
细胞生物学
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毒理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Jibo Dou,Qiang Huang,Hongye Huang,Defu Gan,Junyu Chen,Fengjie Deng,Yuanqing Wen,Xiaoli Zhu,Xiaoyong Zhang,Yen Wei
A novel ternary composite consisting of Mg/Al layered double hydroxides (LDH), polydopamine (PDA) and poly(methyl vinyl ether- alt -maleic anhydride) (PMVE-MA) was fabricated by a facile combination of mussel-inspired chemistry and a ring-opening reaction. Dopamine can serve as a “minimalist mimic” of mussel adhesive protein to form a layer of polydopamine (PDA) on the LDH surface under rather mild conditions (including air atmosphere, aqueous solution, and catalyst free). Subsequently, the PMVE-MA brushes were immobilized onto the PDA modified LDH via a ring-opening reaction. The morphology and chemical compositions of the as-prepared samples were characterized by SEM, TEM, FT-IR, TGA , and XPS. To evaluate the adsorption performance of the PMVE-MA modified LDH ( [email protected] @PMVE-MA) composites, the obtained samples were used as adsorbents for the removal of copper ions (Cu 2+ ) from an aqueous solution. The results demonstrated that the [email protected] @PMVE-MA composites showed a significant improvement in the adsorption efficiency towards Cu 2+ , and the adsorption capacity of the [email protected] @PMVE-MA composites was found to be 2 times higher than that of pristine LDH. Adsorption kinetics showed that the experimental data were fitted well by the pseudo-second-order kinetic model. Equilibrium data could be best described by the Langmuir isotherm model, with the maximum monolayer adsorption capacity of 193.78 mg/g. Thermodynamic studies indicated that the adsorption of Cu 2+ onto the [email protected] @PMVE-MA composites is an endothermic and spontaneous process. Importantly, it can be easily regenerated by low-cost reagents, and exhibited high removal efficiencies after four cycles of adsorption-desorption. These results suggest that the [email protected] @PMVE-MA nanocomposites are good candidate for Cu 2+ removal from aqueous solutions.
研究领域:
材料科学
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环境化学
·
表面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER SCIENCE SERIES A
·
2017
·Vol. 59
·期 5
·708-717
·
IF 0.822
Zhang Q. L.,Tian X. H.,Sun J. Y.,Yuan Y. Z.,Zhang K. T.
Nanoparticles including starch- graft -methylmethacrylate, starch- graft -(methylmethacrylate/methyl acrylate), starch- graft -(methyl methacrylate/butyl methacrylate) were synthesized via emulsifier-free emulsion polymerization and were blended with natural rubber latex at various mass ratios. Chemical structure of graft copolymers was confirmed by Fourier transform infrared. Transmission electron microscopy demonstrated the core-shell structures of the nanoparticles distributed uniformly around the natural rubble particles. The tensile strength of blend films was significantly enhanced by addition of graft copolymers. Besides, scanning electron microscopy and atomic force microscopy showed the blend film had smooth surface.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2025
·Vol. 18
·期 17
·4163
·
IF 3.2
Fei Gao,Peng Xu,Yongqing Zhang,Hui Zhang
To address scaling issues in oilfield water injection, a degradable terpolymer scale inhibitor MA-AA-AMPS (terpolymer)was synthesized via aqueous solution polymerization using maleic anhydride, acrylic acid, and 2-acrylamido-2-methylpropanesulfonic acid as monomers. Characterization confirmed the presence of carboxyl, sulfonic, and amide groups in the copolymer with good thermal stability. Scale inhibition tests showed that at 2% dosage, its scale inhibition efficiency exceeded 80%, remaining above 80% in the pH range of 3–8 and over 50% at 150 °C, with excellent tolerance to high-calcium environments. Biodegradation tests revealed BOD5/COD > 0.3, with a biodegradation rate exceeding 50% in 15 days and reaching 83.4% in 30 days, indicating environmental friendliness. This scale inhibitor effectively solves scaling problems in oilfield water injection systems.
研究领域:
石油工程
·
高分子化学
·
环境科学
·
阻垢技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Stem Cell Research & Therapy
·
2022
·Vol. 13
·期 1
·1-15
·
IF 8.079
Liu Fengyi,Wang Xu,Li Yuzhou,Ren Mingxing,He Ping,Wang Lu,Xu Jie,Yang Sheng,Ji Ping
Background Cartilage defects pose a significant burden on medical treatment, leading to an urgent need to develop regenerative medicine approaches for cartilage repair, such as stem cell therapy. However, the direct injection of stem cells can result in insufficient delivery or inaccurate differentiation. Hence, it is necessary to choose appropriate stem cell delivery scaffolds with high biocompatibility, injectability and chondral differentiation induction ability for cartilage regeneration.Methods In this study, the photocrosslinked gelatin methacrylate (GelMA) hydrogel with high cell affinity and plasticity was selected and strengthened by incorporating methacrylic anhydride-modified poly(amidoamine) (PAMAM-MA) to fabricate an adipose-derived stromal/stem cells (ASCs) delivery scaffold for cartilage repair. The physiochemical properties of the GelMA/PAMAM-MA hydrogel, including the internal structure, stability and mechanical properties, were tested. Then, ASCs were encapsulated into the hydrogels to determine the in vitro and in vivo chondrogenic differentiation induction abilities of the GelMA/PAMAM-MA hydrogel.Results Compared with the GelMA hydrogel, the GelMA/PAMAM-MA hydrogel exhibited more uniform structure, stability and mechanical properties. Moreover, on the basis of good biocompatibility, the hybrid hydrogel was proven to exert a sufficient ability to promote cartilage regeneration by in vitro three-dimensional (3D) culture of rASCs and in vivo articular cartilage defect repair.Conclusion sThe injectable photocrosslinked GelMA/PAMAM-MA hydrogel was proven to be a capable stem cell carrier for cartilage repair and provides new insight into the design strategy of stem cell delivery scaffolds.
研究领域:
再生医学
·
组织工程
·
干细胞生物学
·
生物医学材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Siru Liu,Haixiao Guo,Yufen Wang,Jiaqi Hou,Tingting Zhu,Yiwen Liu
Iron-based peracetic acid (PAA) advanced oxidation process (AOP) is widely used in water purification because of its high efficiency and low toxicity. In this study, for the first time, ferrous iron (Fe 2+ ) and PAA were dosed jointly into the rising main sewer reactor, to verify the feasibility of sulfide and methane control as well as investigate the comprehensive mechanism of Fe 2+ /PAA on sewer biofilm. Results demonstrated the superior biocidal effect of Fe 2+ /PAA dosing than that of PAA alone. Intermittent Fe 2+ /PAA dosing showed that the average inhibitory rate of sulfide production rate (SPR) and methane production rate (MPR) was 52.0% and 29.9%, respectively, at a Fe 2+ /PAA molar ratio of 1:1 and PAA concentration of 3 mmol/L (i.e., the mass-based concentrations of Fe 2+ and PAA were 6.79 mg-Fe/L and 228 mg/L, respectively). Beside, sewer biofilm was found to be resistant to PAA during repeated dosing events. However, resistance could be alleviated by introducing sulfide in situ in the Fe 2+ /PAA process, and SPR and MPR were further reduced to 27.39% and 67.32% of the control, respectively. LIVE/DEAD Staining showed that Fe 2+ /PAA exhibited a strong destructive effect on microbial cells, with the proportion of viable cells being 26.34%. Electron paramagnetic resonance (EPR) and free radical quenching results indicated that the inhibitory order was R-O• > •OH > Fe(IV), which led to the disruption of cellular integrity (i.e., 17.24% increase in LDH) and intracellular enzyme system (i.e., cellular metabolic disorders). Microbial analysis revealed that long-term Fe 2+ /PAA dosing decreased the sulfate-reducing bacteria (SRB) abundance, and the dominant genus of methanogenic archaea (MA) shifted from Methanofastidiosum, Methanobacterium to Methanosaeta . The cost of Fe 2+ /PAA dosing on methane and sulfide control in rising main sewers was $1.81/kg-S, economically and environmental-friendly attractive for practical applications.
研究领域:
环境工程
·
水处理
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2020
·Vol. 11
·期 35
·5619-5629
·
IF 5.342
Yechun Zhou,Zhe Wang,Yuqing Wang,Lishan Li,Nianchen Zhou,Yuanli Cai,Zhengbiao Zhang,Xiulin Zhu
Azobenzene-based polymeric nanoparticles have emerged as promising candidate for biological detection and controlled drug release in an anaerobic environment for colon disease treatment. Herein, we report a novel and efficient polymerization-induced self-assembly (PISA) strategy for fabricating polymeric nanovehicles to load drugs for triggered release and fluorescence detection based on a TPE (tetraphenylethylene)-azobenzene fluorescence probe sensitive to hypoxic microenvironments. In this study, a series of PISA-nanoparticles, including micelles and vesicles, were synthesized by reversible addition–fragmentation transfer (RAFT) dispersion copolymerization of monomers butyl methacrylate (BMA) and reductive-responsive TPE-AZO-MA (TPE-azobenzene derived methacrylate) in a 1,4-dioxane/water mixture. Subsequently, polymeric micelles loaded with doxorubicin (DOX) in situ were successfully obtained by PISA. Furthermore, the release and fluorescence change of PISA-formed micelles loaded with DOX were studied in a simulated colon environment under the action of azoreductase. As expected, the drug-loaded micelles showed an effective DOX release during enzyme hydrolysis within 24 h owing to azo bond cleavage. Meanwhile, significant fluorescence enhancement was observed, accompanied by the release process. This was due to the aggregation-induced emission (AIE) performance of TPE being efficiently activated by elimination of the fluorescence resonance energy transfer (FRET) process, also owing to azo bond cleavage. Finally, the cytotoxicity and cellular uptake of the blank micelles and DOX-loaded micelles in vitro were evaluated.
研究领域:
药学
·
材料科学
·
纳米技术
·
生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 25
·10359-10369
·
IF 4.5
Liu Shujuan,Liu Lijuan,Yang Kangli,Yuan Zhiqing,Li Xinyi,Li Cancheng,Meng Shoutong
In order to improve the environmental problems caused by the extensive use of plastics, this paper introduced a new strategy to add and modify the physical structure, which could not only reduce the use of plastics, but also improve the mechanical properties of bamboo plastic composites. In this work, the bamboo-woven structure was introduced into the bamboo fiber/low density polyethylene (LDPE) composites to enhance the mechanical properties. Besides, to enhance the interfacial compatibility, maleic anhydride (MA) and polyvinyl alcohol (PVA) were used to modify LDPE and bamboo-woven structure, respectively. The mechanical properties of the prepared PVA-bamboo-woven structure/bamboo fiber/MA-LDPE composites were greatly improved. The bamboo plastic composites were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, water contact angle (WCA) test and mechanical property test. The results showed that the bamboo-woven structure/bamboo fiber/MA-LDPE composite has superior properties at 45% bamboo fiber content. The tensile strength reached to 33.842 MPa, which was increased by 65.01% compared to the bamboo fiber/MA-LDPE composites, and the bending strength reached to 36.6585 MPa increased by 81.46% in relation to the bamboo fiber/MA-LDPE composites. In addition, even a large number of hydrophilic bamboo fibers and bamboo-woven structure were carried by the composites, a good hydrophobicity was still maintained and the WCA reached to 93.71°. The composites with excellent mechanical properties and hydrophobicity provided a reference for the use of bamboo fibers in environmentally friendly packaging materials.
研究领域:
材料科学
·
高分子科学
·
环境工程
·
生物复合材料
·
机械工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）