COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Shihong Zhu,Daohang Du,Shuli Yu,Ting Tao,Xianfeng Chu,Yunhua Jiang,Jia Yang,Jiefu Li,Zhifei Wang
Chemiluminescent immunoassays utilizing magnetic nanoparticles exhibit critical importance in in vitro diagnostics, attributed to their remarkable superparamagnetic properties, high specificity, and uniform monodispersity. Current surface functionalization strategies for antibody conjugation predominantly rely on carboxyl or amino groups, requiring pre-activation steps for both amino- and carboxyl-modified nanoparticles prior to antibody labeling. However, the complex activation protocols and persistent challenges in developing efficient chemiluminescent detection methods highlight significant technical obstacles. To address these limitations, we developed vinyl sulfone-functionalized magnetic beads (VSF-GMP), enabling rapid antibody conjugation via specific reactions between vinyl sulfone groups and lysine residues exposed on the surface of creatine kinase MB isoenzyme antibodies (CK-MB). This approach facilitates highly efficient chemiluminescent immunoassays. The engineered VSF-GMP magnetic beads demonstrated molecular weight-dependent adsorption profiles, achieving maximal binding capacity (86.32 μg/mg) for mouse IgG (150 kDa) compared to β-lactoglobulin (18.2 kDa) and bovine serum albumin (66.4 kDa). Moreover, compared with the protein coupling method using EDC/NHS-activated carboxylated magnetic beads, our VSF-GMP beads exhibited superior coupling efficiency. Additionally, bis-functionalized poly(ethylene glycol) (NH 2 -PEG-NH 2 ) was employed as a blocking agent to optimize the reactivity and stability of antibody conjugation, reducing non-specific protein labeling on the magnetic beads by 97.8 %. The analytical performance of the proposed chemiluminescent immunoassay (CLIA) was rigorously evaluated. Finally, the practical utility of VSF-GMP magnetic beads for CK-MB determination in plasma samples was investigated. These engineered magnetic beads showcase exceptional analytical sensitivity, specificity, and operational simplicity, establishing a robust platform for advanced chemiluminescent diagnostics with enhanced throughput capabilities.
研究领域:
生物医学工程
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分析化学
·
免疫学
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产品关联
DOI
高影响因子
Materials
·
2021
·Vol. 14
·期 11
·3040
·
IF 3.623
Wei Zhang,Kaikai Wang,Xiaodan Hu,Xiaohong Zhang,Shuquan Chang,Haiqian Zhang
A shortage in the supply of3He used for thermal neutron detector makes researchers to find3He alternatives for developing new neutron detectors. Here, we prepared a neutron-sensitive composite liposome with tributyl borate and encapsulating with Fe3O4@oleic acid nanoparticles (Fe3O4@OA NPs), methylene blue (MB), or anti-albumin from bovine serum (anti-BSA). The tributyl borate compound was characterized by Fourier transform infrared spectroscopy (FT-IR). In addition, the morphology, element compositions, and magnetic properties of the composite liposome were investigated with transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), and vibrating sample magnetometer (VSM), respectively. The results indicated that a typical ellipsoidal magnetic liposome structure was obtained, and the lengths of the minor axis and major axis were 49 ± 1 nm and 87 ± 3 nm, respectively. Under thermal neutron irradiation, the structure of composite liposome was destroyed, and encapsulated reporter molecules were released, which was detected by ultraviolet–visible (UV–vis) spectroscopy and surface plasmon resonance (SPR) technology. The response of this sensor based on a destructive assay shows a good correlation with neutron doses. Besides, the sensor has a neutron to gamma-ray rejection ratio of 1568 at a thermal neutron flux rate of 135.6 n/cm2·s, which makes it a promising alternative to3He.Keywords:neutron detector;magnetic liposome;methylene blue (MB);anti-BSA;surface plasmon resonance
研究领域:
核物理
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材料科学
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纳米技术
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辐射检测
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Ke Yang,Xiang Yang,Xiang Zhao,Marc Lamy de la Chapelle,Weiling Fu
Simple, rapid, and efficient cell viability assays play a fundamental role in much of biomedical research, including cell toxicology investigations and antitumor drug screening. Here, we demonstrate for the first time a rapid and label-free cell viability assay using THz spectroscopy in combination with a new optical clearing agent (OCA) and microfluidic technology. This strategy uses a considerably less absorptive OCA to replace the highly absorptive water molecules around the living cells and thus to decrease the background signal interference. Three low-viscosity oils were screened as potential OCA candidates, among which fluorinated oil was selected because of its lower absorption and lowest cytotoxicity. After the liquid medium was replaced with fluorinated oil in a microfluidic chip, an obvious THz spectral difference was observed between the fluorinated oils with and without living cells. This change in THz response was preliminarily attributed to the distinguishable signals between the cells and the fluorinated oil. In addition, we applied this method to cell viability assays of human breast cancer cells (MDA-MB-231) after treatment with different antitumor drugs. The results indicated that THz spectroscopy with the aid of the proposed water-replacement strategy presented excellent quantification of cell viability with the advantages of a rapid, label-free, nondestructive microassay, which offers significant potential to developing a convenient and practical cell analysis platform.
研究领域:
生物医学工程
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细胞生物学
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药学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 4
·e202201203
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IF 2.9
Weirong Song,Jie Zhang,Pengxiang Zhao,Yongchun Huang,Shoude Zhang
A novel monoterpene alkaloid, named incarvine G, was isolated from the Incarvillea sinensis Lam. Its chemical structure was elucidated using comprehensive spectroscopic methods. Incarvine G is an ester compound comprised of a monoterpene alkaloid and glucose. This compound showed evident inhibition on cell migration, invasion, and cytoskeleton formation of human MDA-MB-231 with low cytotoxicity.
研究领域:
药用植物学
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癌症研究
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化学
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产品关联
DOI
PubMed
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2022
·Vol. 31
·期 6
·936-948
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IF 2.351
Hu Shijia,Li Yunqi,Kan Weiqiong,Ding Tingting,Gu Haijun,Zhang Ting,Yi Zhengfang,Chen Yihua
A series of diphenyl- N -heteroaromatic compounds were designed, synthesized, and evaluated for anticancer efficacies (antimigration and antiproliferation) against human cancer cell lines. The favorable 2 H -1,2,3-triazole compound 4a was optimized, leading to the discovery of compounds 4b and 4c with more potent antimigratory and antiproliferative activities. The transwell assay showed that compounds 4b and 4c dose-independently inhibited the migration of the two cancer cell lines (human breast cancer cell line MDA-MB-231 and pancreatic cancer cell line PANC1) with the low IC 50 values of 0.24 and 0.47 µM, respectively. The sulforhodamine-B (SRB) assay showed that compounds 4b and 4c inhibited the proliferation of PANC1 cells with the IC 50 values 0.86 µM and 1.41 µM, respectively. These results indicate that compounds 4b and 4c with 1,2,3-triazole scaffold may be further developed as potent anticancer agents in the future.
A series of novel diphenyl- N -heteroaromatics based on our previous published research work were synthesized and evaluated for their antimigration efficacies against MDA-MB-231 and PANC1 human cancer cell lines. Compound 4c dose-independently inhibited the migration of the two cancer cell lines (MDA-MB-231 and PANC1) with low IC 50 values 0.32 µM and 0.24 µM respectively.
研究领域:
药理学
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肿瘤学
·
有机化学
✓ 已验证
DOI
高影响因子
ONCOLOGY REPORTS
·
2020
·Vol. 44
·期 5
·2231-2240
·
IF 3.417
Jinhong Feng,Ting Zheng,Zhaohua Hou,Cui Lv,Anqi Xue,Tingting Han,Beibei Han,Xin Sun,Yunbo Wei
Estrogen receptor (ER)‑negative breast tumors are associated with low survival rates, which is related to their ability to grow and metastasize into distal organs. The aryl hydrocarbon receptor (AhR), a ligand‑activated transcription factor that is involved in several biological processes, is a promising anti‑metastatic target. Luteolin, a non‑toxic naturally occurring plant flavonoid with diverse biological activities, has been demonstrated to be effective against certain types of cancer, and has also been described as a ligand of AhR. In the present study, various cancer cell lines were first investigated following treatment with luteolin, and luteolin exhibited the lowest IC50 in MDA‑MB‑231 cells. Then, the efficiency of luteolin in suppressing the metastasis of ER‑negative breast cancer in vitro was assessed. MDA‑MB‑231 cells were treated with luteolin in vitro. Subsequently, MTT assay and flow cytometry were used to detect cell viability, the cell cycle and apoptosis, and a Transwell assay was used to evaluate cell invasion. In addition, reverse transcription‑semi‑quantitative PCR and western blot were performed to detect the mRNA and protein expression levels of matrix metalloproteinase (MMP)‑2 and MMP‑9. In addition, the number of surface tumor nodules was measured in vivo, in mice bearing B16‑F10 tumors, following treatment with luteolin. Luteolin inhibited the viability and induced the apoptosis of MDA‑MB‑231 cells, which was accompanied by cell cycle arrest. This was associated with a decrease in the expression of the pro‑metastatic markers C‑X‑C chemokine receptor type 4 (CXCR4), MMP‑2 and MMP‑9, which was reversed by AhR inhibition. Furthermore, it was identified that luteolin could inhibit the metastasis in a B16F10 mouse xenograft model, and the levels of MMP‑9, MMP‑2 and CXCR4 were significantly decreased in the lung tissues isolated from tumor‑bearing nude mice following luteolin treatment. In conclusion, luteolin is a potential molecule for inhibiting breast cancer invasion and metastasis, which could have promising clinical applications.
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Quan Chen,Lunan Wu,Xiaoli Zhao,Xiu-Jie Yang
Zn-Ti layered double oxide (LDO) nanosheets were synthesized by a simple co-precipitation-calcination process for the enhanced photocatalytic degradation of methyl orange (MO), rhodamine B (RhB) and methylene blue (MB). ZnTi-LDOs nanosheets present higher photocatalytic efficiency for MO, RhB and MB removal, which is 5.4, 4.4 and 6.7 times of that of Zn-Ti layered double hydroxide (LDH), respectively. The photocatalytic activity of synthesized ZnTi-LDOs is greatly affected by the mole ratio of Zn/Ti, calcination temperature and pH value. It is found that the formation of ZnO/ZnTiO 3 heterojunction in ZnTi-LDOs could effectively accelerate the rapid separation of charge carriers, and thus enhance the photocatalytic activity via enlarging the photoresponse range. The possible charge transfer mechanism for photocatalytic degradation of organic dyes by ZnTi-LDOs was proposed based on the active species test and UV–Vis diffuse reflectance spectra. The results of active species trapping experiments showed that ‧OH, ‧O 2 − , h + , e − all had effect on the degradation of RhB , and ‧OH played a major role. This study provides a new insight for the photocatalytic degradation of organic pollutants from wastewater by ZnTi-LDOs.
研究领域:
材料科学
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环境科学
✓ 已验证
DOI
高影响因子
ACS Catalysis
·
2016
·
IF 9.307
Jian-Ping Zou,Dan-Dan Wu,Jinming Luo,Qiu-Ju Xing,Xu-Biao Luo,Wen-Hua Dong,Sheng-Lian Luo,Hong-Mei Du,Steven L. Suib
A strategy was developed to couple photocatalytic oxidation with photocatalytic reduction technology to realize one-pot conversion of MB into hydrocarbons for the first time. In this approach, organic pollutants were first decomposed into CO2 by photodegradation and then the as-obtained CO2 was converted into CH3OH, C2H5OH, and CH4 through photocatalytic reduction of CO2 under solar spectrum irradiation by using GQDs/V-TiO2 catalysts. The experimental results show that 5%GQDs/V-TiO2 has the best photocatalytic activity and the product rates of CH3OH, C2H5OH, and CH4 are 13.24, 5.65, and 0.445 μmol g–1 h–1, respectively. The corresponding apparent quantum efficiency is 4.87% at 420 nm. The one-pot conversion of MB into hydrocarbons was demonstrated by a series of experiments. The photocatalytic mechanisms of one-pot conversion of MB into hydrocarbons were proposed to explain the detailed photocatalytic process.
研究领域:
环境科学
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化学工程
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材料科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Zhu Mengting,Tonni Agustiono Kurniawan,Song Fei,Tong Ouyang,Mohd Hafiz Dzarfan Othman,Mashallah Rezakazemi,Saeed Shirazian
Methylene blue (MB) is a dye pollutant commonly present in textile wastewater. We investigate and critically evaluate the applicability of BaTiO 3 /GO composite for photodegradation of MB in synthetic wastewater under UV–vis irradiation. To enhance its performance, the BaTiO 3 /GO composite is varied based on the BaTiO 3 weight. To compare and evaluate any changes in their morphologies and crystalline structures before and after treatment, BET (Brunauer–Emmett–Teller), XRD (X-ray diffraction), FTIR (Fourier transform infrared spectroscopy), SEM (scanning electron microscopy) and TEM (transmission electron microscopy) tests are conducted, while the effects of reaction time, pH, dose of photocatalyst and initial MB concentration on its photodegradation by the composite are also investigated under identical conditions. The degradation pathways and removal mechanisms of MB by the BaTiO 3 /GO are elaborated. It is evident from this study that the BaTiO 3 /GO composite is promising for MB photodegradation through ·OH. Under optimized conditions (0.5 g/L of dose, pH 9.0, and 5 mg/L of MB concentration), the composite with 1:2 dose ratio of BaTiO 3 /GO has the highest MB degradation rate (95%) after 3 h of UV vis irradiation. However, its treated effluents still could not comply with the discharge standard limit of less than 0.2 mg/L imposed by national environmental legislation. This suggests that additional biological treatments are still required to deal with the remaining oxidation by-products of MB, still present in the wastewater samples such as 3,7-bis (dimethyl-amino)-10H-phenothiazine 5-oxide.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2023
·
IF 4
Ye Haiyang,Yu Kun,Li Bing,Guo Jianzhong
Heavy metal pollution and organic wastewater treatment have attracted extensive attention of researchers. Adsorption method is widely used in wastewater treatment because of its low price and high efficiency. As a natural, cheap biomass material, bamboo powder is of great significance to develop it into an economical and efficient biochar for wastewater treatment. A new type of ball-milled biochar (BMBC) adsorbent was prepared by pyrolysis of bamboo powder under an inert atmosphere, activated with sodium hydroxide solution and ball milling. The specific surface area of BMBC increased from 29.21 to 60.49 m 2 /g, and the carboxyl and hydroxyl groups on the pyrolyzed carbon surface also obviously increased after ball milling. The results of bath adsorption experiments showed that BMBC exhibited excellent adsorption capacity towards both Pb(II) (170.52 mg/g) and methylene blue (MB, 419.11 mg/g) at 308 K. Based on a systematic study on the effect of various working conditions (including concentration, temperature, pH value, and ionic strength), it was demonstrated that pseudo-second-order kinetic equation might match the adsorption process ( R 2 > 0.9934) and Langmuir model ( R 2 > 0.9924); adsorptions were thermodynamically endothermic and occurred spontaneously. The ball milled alkalization-modified bamboo biochar can potentially be applied to remove heavy metal and cationic pollutants from wastewater.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 17
·8170-8181
·
IF 3.3
Shuanghui Liu,Changle Wang,Yao Song,Binglin Yan,Bing Ai,Kefeng Pan,Lipeng Zhang
The heterojunction structure of semiconductor photocatalysts is significant for making full use of their capabilities for organic molecules and dye degradation. In this paper, we present a heterogeneous junction composite fabricated with hybrid phase TiO2 and g-C3N4 for methylene blue (MB) removal through adsorption and photocatalytic degradation. The heterogeneous junction system was prepared by hydrothermal treatment with a subsequent pyrolysis process. The variation of the proportion and lattice structure of hybrid phase TiO2 in the synthesized catalysts was analyzed by refinement. Adsorption experiments showed that the maximum adsorption capacity of TiO2/g-C3N4 (1 : 10) was 1667 mg g−1, which was 45.97 times and 63.78 times higher than that of TiO2 and g-C3N4, respectively. In the photodegradation process, the TiO2/g-C3N4 (1 : 10) photocatalyst shows a removal rate of MB of 100 mg L−1, which was as high as 96.262%, significantly higher than that of pure TiO2 and g-C3N4. Brunner–Emmett–Teller (BET) measurements and Fourier transform infrared spectroscopy (FTIR) showed that the adsorption of MB onto TiO2/g-C3N4(1 : 10) was chemical adsorption, and ˙OH, which acts as an active substance, was found to play a major role in the photocatalytic degradation process. Overall, the developed hybrid phase TiO2/g-C3N4 heterojunction composite may be beneficial for the treatment of water pollution.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yao Wang,Dan Li,Junfeng Kang,Shiye Guan,Daoxin Wu
In this work, graphene (GR) and Ag-ZnO nanocomposites were prepared and used together with methylene blue (MB) as electrode materials to modify a glassy carbon electrode (GCE). The electrochemical behavior of L-tryptophan (L-Trp) on the fabricated MB/Ag-ZnO/GR/GCE was investigated by cyclic voltammetry and differential pulse voltammetry. MB/Ag-ZnO/GR clearly enhanced the electrochemical oxidation of L-Trp. In a 0.1 mol/L phosphate buffer solution at pH 4.0, the peak current response at the modified electrode increased linearly with an increase in the L-Trp concentration from 2.0 x 10 -6 to 1.4 x 10 -4 mol/L, and the detection limit (S/N = 3) was 1.0 × 10 -6 mol/L. The electrode showed good reproducibility, stability, and selectivity. The sensor was used for the determination of L-Trp in biofluid samples (pig serum and pig urine), and the recovery rate ranged from 97.1% to 104.2%. The fabricated electrode was proposed for the electrochemical analysis of L- Trp, indicating the potential for practical applications in the biological field.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）