ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Yu Luyao,Liu Meichen,Niu Ziyi,Zhang Jiarui,Deng Yue,Zhou Xinyue,You Jiansong,Xue Hongyu,Yin Lei,Shi Meiyun
Monodisperse polyethylene glycol (PEG) derivatives offer significant advantages over conventional polydisperse PEGs for biomedical applications due to their precisely defined molecular structures. This study establishes an eco-efficient, selective, and sensitive analytical assay integrating microscale solid-phase extraction (μ-SPE) with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to investigate the cellular uptake of HO-PEG 8 -OH polymers in MCF-7 cells. The method achieved greater than 91% recovery from 20 μL lysates using M-PEG 6 -OH as the internal standard, with validated linearity (10–1000 ng/mL, R > 0.997), accuracy (relative error < ± 7.49%), and precision (RSD < 7.50%). The novelty of the assay is the harmonization of analytical performance with green analytical chemistry principles. The validated method provides a robust platform for studying monodisperse PEG derivatives while addressing growing demands for sustainable analytical technologies. Green analytical chemistry metric assessments confirmed the environmental sustainability of the method. Cellular pharmacokinetic analysis revealed time-/concentration-dependent uptake kinetics of HO-PEG 8 -OH polymers in MCF-7 cells, showing 3.2-fold accumulation between 0.5 and 48 h exposure. These findings offer important insights for PEG-based drug delivery system optimization and establish a new standard for environmentally conscious bioanalytical method development. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2021
·
IF 4.833
Zhihuan Zhao,Pengfei Yang,Xiao Zhang,ShashaYang,Jianying Lin,Jimin Fan,Bing Zhang
MoS 2 /C@SiO 2 spheres were successfully synthesized by a one-step hydrothermal method using silica as the template, sodium molybdate as the Mo source and glucose as the carbon precursor. In this paper, the antitumor ability of MoS 2 /C@SiO 2 and C@SiO 2 nanospheres was evaluated through the photothermal effect induced by near infrared (NIR) spectroscopy and the drug loading and release behaviors of the model drug doxorubicin (DOX). The photothermal conversion efficiency (η) of MoS 2 /C@SiO 2 of 42.5% was 1.2 times that of C@SiO 2 (34.7%), which indicated its significant photothermal effect. The drug loading of MoS 2 /C@SiO 2 of 46.5% was much higher than that of C@SiO 2 (12.4%); it was 3.75 times that of C@SiO 2 . The cumulative drug release of MoS 2 /C@SiO 2 and C@SiO 2 under a simulated acidic tumor environment and NIR irradiation was 58.9% and 27.29%, respectively. Moreover, the cell viability assays verified that MoS 2 /C@SiO 2 -DOX had excellent antitumor ability under the co-stimulation of endogenous (pH) and exogenous (NIR) compared with C@SiO 2 -DOX. The experimental results showed that the survival rate of cancer cells in MoS 2 /C@SiO 2 -DOX under coordinated treatment was only 19.4%, while that of C@SiO 2 -DOX was 24.6%.
研究领域:
生物医学工程
·
纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 7
·期 6
·974-985
·
IF 4.776
Abid Hussain,Shengrong Guo
To prevent premature drug release from nanoparticles, it is vital to design and prepare controlled and site-specific drug release systems. We investigated a new controllable drug release mesoporous carbon nanoparticle (MCN)-based nano-system with the advantages of enhanced permeability and retention effect (EPR) to target tumors followed by the NIR-induced heat-triggered release of a chemotherapeutic drug to show anti-tumor effects. The pores of MCN with photo-thermal effects were filled with the chemotherapeutic agent doxorubicin (DOX) and the phase change material (PCM) 1-tetradecanol was used as a gatekeeper to trap DOX inside the pores of the mesoporous carbon nanoparticles and the release of DOX inside tumor cells was triggered using NIR irradiation. The surface of MCN was coated with natural sophorolipid (SLPD) to obtain nanoparticles (DOX-PCM@MCN-SLPD) with good biocompatibility, dispersibility and stability in aqueous solutions. The MCN-based nano-system had the ability to load 24% DOX trapped with the PCM before coating with SLPD. It was observed that the release of DOX was temperature-dependent above the melting point of PCM. Once DOXPCM@MCN-SLPD was delivered into MCF-7/ADR cells, the release of DOX was triggered by MCN-borne photo-thermal effects under NIR irradiation. The amount of DOX inside the tumor was visualized via confocal laser scanning microscopy, which showed a higher amount of DOX at a higher temperature compared to that at normal body temperature, further confirming the minimization of premature drug release at 37 °C.
研究领域:
药剂学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2015
·
IF 0.476
S.E. Eltayeb,Zhigui Su,Yanyu Xiao,Qineng Ping
Diverse lines of research show that the cellular response to artemether (ART) is multi-factorial in nature. The cytotoxicity of ART is specific to cancer cells because most of them over expressed; transferrin receptors and have high level of intracellular iron and ART is mainly toxic after; interaction with iron ion. Our aim is to investigate the effect of some formulation characteristics on; the cytotoxicity of ART in C6 and MCF-7 cells lines. In this study, the cytotoxicity of ART-loaded; anionic, cationic or neutral (transferrin modified) lipid nanospheres was studied by MTT and; apoptosis tests. Characterizations of apoptosis were done. The effect on the mitochondria and the; nucleus was qualitatively characterized after using different types of cell tracker dyes. The cellular; uptake, accumulation and distribution of the formulations were characterized after loading; different hydrophobic fluorescence probes instead of ART. The relation between the; accumulated amount of ART and its cytotoxicity was defined. Our study shows that ART can be highly toxic to the tumor cells if accumulated in a large amount in the cell. Lipid nanosphere containing tween80 and transferrin can highly accumulate ART in brain cells and can be; formulated as a promising potent, safe and inexpensive drug carriers for brain tumors.
研究领域:
药理学
·
肿瘤学
·
纳米技术
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Jing Li,Na Fan,Xin Wang,Zhonggui He
The present work initially evaluated cellular toxicity and uptake of our previous biomimetic bimodal nanoporous silica (B-BNS) and applied it as lysozyme adsorbent, which aimed to study potential ability of B-BNS as antitumor biological macromolecules carrier. To highlight the advantage of bimodal mesopores, comparisons were made between single mesoporous silica nanoparticles (S-MSN) and B-BNS. Cell evaluation work was conducted using MCF-7 cells and lysozyme adsorption process was studied with pH and lysozyme concentration as independent variables. The results indicated that the toxicity of S-MSN and B-BNS on MCF-7 cell could be neglected. In addition, S-MSN and B-BNS had the ability to be uptaken into cells and even nucleus evidenced by inverted fluorescence microscope and confocal laser scanning microscopic. Compared to S-MSN, B-BNS adsorbed larger amount of lysozyme due to its bimodal mesopores. Lysozyme adsorption was favorably approximated by the pseudo-second order model. The equilibrium data of lysozyme adsorption were fitted to the Langmuir isotherm model much better than the Freundlich isotherm model, suggesting that lysozyme adsorption on B-BNS via the monolayer adsorption process. Overall, B-BNS can be considered as good antitumor biological macromolecules carrier.
研究领域:
材料科学
·
生物医学工程
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2013
·Vol. 15
·期 12
·1-11
·
IF 2.175
Li Zhi,Zhang Fei-long,Wang Zhiyuan,Pan Li-li,Shen Ying-ying,Zhang Zhen-zhong
The photocytotoxicity of water-dispersed 100–300 nm fullerene amino acid derivatives nanoparticles was studied. The nanoparticle solution of fullerene derivatives, l -phenylalanine (C60-phe) and glycine (C60-gly), suppressed the in vitro growth of MCF-7 cells lines, induced cancer cells apoptosis, and caused a perturbation of the cell cycle. These nanoparticle solutions increased intracellular reactive oxygen species after irradiation. C60-phe or C60-gly upregulated the expression of phosphorylated (p)p38 mitogen-activated protein kinase (MAPK). N -Acetyl- l -cysteine significantly depressed the composite-induced activation of p38MAPK, and the kinase inhibitor SB203580 significantly prevented C60 derivative-induced cell apoptosis. This study revealed that p38MAPK is activated by C60 nanoparticles through triggering reactive oxygen species generation, leading to cancer cell injuries.
研究领域:
纳米技术
·
癌症生物学
·
细胞生物学
·
药理学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 19
·14858-14870
·
IF 3.84
Qingqing Yang,Lian Li,Xi Zhu,Wei Sun,Zhou Zhou,Yuan Huang
Thanks to the enhanced cancer cell affinity, modification of polymeric drug carriers with an active targeting ligand has been one of the most effective strategies to achieve better therapeutic efficacy. However, hydrophobic ligands (e.g. folate), when conjugated to hydrophilic carriers, encounter the problem of reduced targeting effectiveness due to the fact that the ligand may be buried inside the carrier. In the present study, we studied the impact of the hydrophilic polymer structure on the targeting efficiency of the conjugated folate (FA). After modification with folate, the linear N-(2-hydroxypropyl)-methacrylamide (HPMA) polymer (P-FA, 27.3 kDa) exhibited a neutral surface charge (1.5 mV), while the graft HPMA polymer (GP-FA, 117.4 kDa) possessed a mild negative charge (−7.2 mV), which suggests different exposure levels of negatively charged folate on the surface of P-FA and GP-FA. Notably, on folate receptor positive MCF-7 cells, P-FA exhibited comparable cell uptake with the unmodified linear polymer, whereas GP-FA resulted in 2-fold higher cell internalization over the untargeted graft polymer. Meanwhile, the involvement of folate receptor mediated endocytosis and augmented folate binding affinity of GP-FA were observed in comparison with P-FA. Moreover, GP-FA exhibited significantly enhanced tumor accumulation relative to GP, while marginally improved tumor accumulation was observed for P-FA compared with P. In summary, the structure of the hydrophilic polymer plays a vital role in the targeting efficiency of the conjugated hydrophobic ligand.
研究领域:
药学
·
聚合物化学
·
癌症研究
✓ 已验证
DOI
高影响因子
Biomedical Materials
·
2021
·Vol. 16
·期 5
·055001
·
IF 3.715
Junting Tao,Shixuan Feng,Bing Liu,Jiaqi Pan,Chaorong Li,Yingying Zheng
Accurate distinguish of cancer cells through fluorescence plays an important role in cancer diagnosis. Here we synthesized a blue fluorescent nitrogen-doped graphene quantum dots (N-GQDs) from citric acid and diethylamine via one-step hydrothermal synthesis method which was simple and quick to avoid by-products, and highlighted the binding sites to achieve precise combination. Due to the nitrogen element doping, amide II bond was amply obtained and abundant binding sites were provided for hyaluronic acid (HA) conjugation. N-GQDs solution with different pH value was then conjugated to HA via an amide bond for the recognition of human breast cancer cells (MCF-7 cells), and the formation of amide bond was more favorable under alkaline conditions. HA conjugated N-GQDs (HA-N-GQDs) were combined with CD44 which was over expressed on the surface of MCF-7 cells, resulting in MCF-7 cells performing stronger fluorescence. HA-N-GQDs showed high fluorescence, low toxicity, and good cytocompatibility, which held it play a role in fluorescence imaging for accurate identification of cancer cells.
研究领域:
癌症诊断
·
材料科学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Hong Li,Xiaodan Shi,Wei Cui
Asparaginase, widely distributed in bacteria, plants, and mammals, has attracted much attention for the biomedical applications ranging from acute lymphoblastic leukemia, melanomas, colorectal cancer to hematologic cancers. Herein, a facile and simple strategy is developed to fabricate protein assemblies with nano-spherical morphology through electrostatic interactions of asparaginase (Asp) and heparin (Hep). As-prepared nanoparticles exhibited high loading content (43.7%) of Asp while preserving the bioactivity. Importantly, thanks to the anticoagulant activity of the component of Hep, the assembled nanoparticles could effectively avoid the risk of thromboembolism, a common side effect caused by the usage of Asp. Moreover, the in vitro anti-cancer measurement demonstrated that these nanoparticles showed dose-dependent inhibitory effect towards MCF-7 cells in 1640 medium. These results pave the way to guide protein assembly with enhanced anti-cancer efficacy and reduced side effects.
研究领域:
生物医学工程
·
癌症研究
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Chen Zhenzhen,Liu Zhihong,Zhang Qian,Huang Sheng,Zhang Zaizhong,Feng Xianquan,Zeng Lingjun,Lin Ding,Wang Lie,Song Hongtao
Drug resistance is a huge hurdle in tumor therapy. Tumor hypoxia contributes to chemotherapy resistance by inducing the hypoxia-inducible factor-1α (HIF-1α) pathway. To reduce tumor hypoxia, novel approaches have been devised, providing significant importance to reverse therapeutic resistance and improve the effectiveness of antitumor therapies. Herein, the nanosystem of bovine serum albumin (BSA)-templated manganese dioxide (MnO2) nanoparticles (BSA/MnO2 NPs) loaded with doxorubicin (DOX) (DOX-BSA/MnO2 NPs) developed in our previous report was further explored for their physicochemical properties and capacity to reverse DOX resistance because of their excellent photothermal and tumor microenvironment (TME) response effects. The DOX-BSA/MnO2 NPs showed good biocompatibility and hemocompatibility. Meanwhile, DOX-BSA/MnO2 NPs could greatly affect DOX pharmacokinetic properties, with prolonged circulation time and reduced cardiotoxicity, besides enhancing accumulation at tumor sites. DOX-BSA/MnO2 NPs can interact with H2O2 and H+ in TME to form oxygen and exhibit excellent photothermal effect to further alleviate hypoxia due to MnO2, reversing DOX resistance by down-regulating HIF-1α expression and significantly improving the antitumor efficiency in DOX-resistant human breast carcinoma cell line (MCF-7/ADR) tumor model. The hypoxia-ameliorated photothermal MnO2 platform is a promising strategy for revering DOX resistance.
研究领域:
癌症治疗
·
纳米技术
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Zhiqiang Bai,Lu Zhao,Haidi Feng,Hui Xu,Nianping Zhang,Yanjun Li,Jinping Song,Yunfeng Bai,Ronghua Yang,Feng Feng
Aptamer (Apt) is a kind of recognition molecule with excellent affinity and high specificity. Transmembrane glycoprotein mucin (MUC1) is an important tumor biomarker overexpressed in MCF-7 tumor cells. MUC1 Apt (Apt-M) could specifically target MCF-7 tumor cells. Ti 3 C 2 nanosheets with high photothermal conversion efficiency were considered as a promising therapeutic nanoplatform for tumor therapy. Doxorubicin (DOX) is a common anti-tumor drug. Herein, a novel DOX/Ti 3 C 2 /Apt-M therapeutic nanoplatform was successfully fabricated, which could specifically target MCF-7 tumor cells. As expected, the temperature of DOX/Ti 3 C 2 /Apt-M therapeutic nanoplatform raised rapidly under laser irradiation. Meanwhile, chemotherapy was triggered through multimodal stimuli-responsive drug release from the DOX/Ti 3 C 2 /Apt-M therapeutic nanoplatform under acidic environment and laser-induced local high temperature. Encouragingly, the MCF-7 cell viability of DOX/Ti 3 C 2 /Apt-M with laser irradiation was only 26.9%. The tumor surface temperature of MCF-7 xenograft mice in the DOX/Ti 3 C 2 /Apt-M + Laser group rose to about 58.3 °C, which was significantly higher than other laser irradiation groups. The experiments in vitro and in vivo indicated that the DOX/Ti 3 C 2 /Apt-M therapeutic nanoplatform can rapidly accumulate in MCF-7 tumors with excellent active targeting properties, and display superior tumor suppressive ability through combination therapy. This work provides a new idea of targeted tumor therapy based on Ti 3 C 2 nanosheets.
研究领域:
纳米技术
·
癌症治疗
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Meiyun Shi,Xinyue Zheng,Yuncheng Ge,Ning Zhang,Luyao Yu,Xujian Duan,Yajun Liu,Hongyu Xue,Jiansong You,Lei Yin
Unraveling the cytotoxicity and cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in cells is important for evaluation of therapeutic efficacy and toxicity of PEGylated drug delivery systems . In this study, cellular uptake of PEG600, PEG2K, PEG4K and PEG10K in MCF-7 cells was first studied by an UPLC-MS/MS assay coupled with collision induced dissociation (CID) in source technique. The CID of PEG in source with high values of declustering potentials generates numerous PEG-related product ions. These PEG-related fragment ions can be further broken into specific product ions in the collision cell as alternative ions for detection of PEG. The quantification of PEG was finally performed with the MRM transition ( m/z 221.0 → 89.0). The experimental results indicated that the toxicity of PEG600, PEG2K, PEG4K and PEG10K was not significant at concentrations of 5–1200 μg/mL and the amounts of PEG polymers entry into MCF-7 cells at was small. The greenness of the developed analytical methods was also assessed by Analytical Eco-Scale, Analytical Greenness calculator (AGREE) and Green Analytical Procedure Index (GAPI) in this study.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）