SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Dongxu Wang,Yanhui Ao,Peifang Wang
Microcystis aeruginosa is one of the most famous harmful algae. In this work, Z -scheme g-C 3 N 4 -MoO 3 (Mo-CN) composite photocatalysts were successfully synthesized to alleviate the algae pollution. The obtained composites exhibited excellent performance for the inactivation of M. aeruginosa . The optimal photocatalysts (15Mo-CN) achieved a removal efficiency of 97% for the algal cells after 3 h visible light irradiation, which was attributed to their remarkable surface properties and ingenious structure design. The physiological status of Microcystis aeruginosa were evaluated by the chlorophyll a (chla), relative electron transport rate (rETR) and maximum photochemical efficiency (Fv/Fm), and all of them decreased in different degrees. Furthermore, the as-prepared photocatalysts also show a certain activity for the removal of leaked macromolecule organic compounds during the process of algal cells inactivation. Finally, a possible mechanism on the photocatalytic inactivation of algal cells by the Z -scheme photocatalysts was deduced based on the experimental and characterization results. We believe this study would provide new insights into the development of promising technology and basic theory for remediation of algae pollution.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Suiliang Huang,Wenwen Kong,Zhenjiang Yang,Hui Yu,Fengyuan Li
The ecological health of aquaculture water is threatened by wasted fish feed and herbicides. In order to study the effect of prometryn and fish feed on Microcystis aeruginosa growth based on Monod and Logistic functions, four different concentrations of prometryn (0, 50, 100 and 200 μg L −1 ) and two different dosages of fish feed (0.075 g, 0.15 g; d < 0.85 mm) were added into the culture medium, and the fish feed was the source of nitrogen and phosphorus in the MII medium. Results showed that Microcystis aeruginosa growth can be fitted well by Logistic and modified Logistic functions with 0–200 μg L −1 prometryn ( R 2 = 0.981–0.998 and R 2 = 0.989–0.999, respectively). With the same concentration of prometryn, the maximum algae density ( N max ) of Microcystis aeruginosa calculated by both Logistic and modified Logistic functions increased with increasing dosage of fish feed and with the same dosage of fish feed, N max declined with increasing concentrations of prometryn. Inhibition of prometryn on algae growth stimulated by fish feed is of double concentration-dependence, inhibition rates ( I ) are lower in 0.15 g fish feed medium than 0.75 g ones generally, implying that more nutrients can alleviate the stress caused by prometryn on algae. Derived formula for the specific growth rate, growth rate and inhibition rate using modified Logistic function agreed reasonably well with measured data. Jointly application of modified Monod and Logistic functions can better describe the relationship between specific growth rates and nutrients concentrations compared to combination of Monod and Logistic functions. In addition, equations for describing variations of nutrients concentrations (PO 4 3- -P and NH 4 + -N) with time were also derived based on both modified Monod and Logistic functions, which agree reasonably well with the measured data. In sum, the combination of modified Monod and Logistic functions provides a promising and robust method in studying algal growth stimulated by fish feed in incubator experiments.
研究领域:
水生生物学
·
环境科学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Mengzi Liu,Yanyan Wei,Muhammad Salam,Xiaobing Yuan,Bingsheng Liu,Qiang He,Xuebin Hu,Hong Li,Yixin He
In this study, a Microcystis aeruginosa -based photobioreactor ( M. aeruginosa -based PBR) was developed for the removal of cadmium (Cd 2+ ) from diluted actual mine wastewater (DW) and Cd 2+ -contained simulated wastewater (SW), with a uniform Cd 2+ concentration of 0.5 mg/L. For the DW and SW, both K + -abundant (DWA & SWA) and K + -insufficient (DWB & SWB) treatments were conducted. It was found that continuous supplementation of K + benefited Cd 2+ removal. The Cd 2+ removal efficiency in SWA reached 70% during the 41 days of operation, which was 20% higher than that in the SWB. The K + addition triggered great higher Cd 2+ removal efficiency (90%) in the DWA in comparison to the SWA. The Cd 2+ assimilation by M. aeruginosa and Cd 2+ retention on M. aeruginosa surface were the primary processes involved in the PBR system. The K + starvation triggered a 45% and 43% loss of M. aeruginosa biomass in the DWA and the DWB, respectively. Hence, the Cd 2+ removal efficiency in DWB increased significantly, and this was attributed to the increased abundance of non-living cells and enhanced bioretention of Cd 2+ . The results revealed that continuous K + supplementation enhanced the Cd 2+ removal efficiency in the M. aeruginosa -based PBR jointly by prompting algal cell growth, Cd 2+ assimilation and biosorption , as well as Cd 2+ retention on the algal cells.
研究领域:
环境工程
·
生物技术
·
水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Weizhen Zhang,Jing Liu,Qi Li,Yunxing Xiao,Yumiao Zhang,Ningfei Lei,Qianchao Wang
Microplastics (MPs) and per- and polyfluoroalkyl substances (PFASs) have drawn significant attention as emerging threats to aquatic ecosystems . There are currently just a few investigations on the combined toxicity of PFAS and MP on freshwater microalgae. In this research, the combined toxicity of polyvinyl chloride (PVC) and perfluorooctanoic acid (PFOA) to Microcystis aeruginosa was investigated. The results indicated that the combination of these pollutants inhibited the growth of M. aeruginosa and promoted the synthesis and release of Microcystin-LR (MC-LR). Individual and combined exposure caused different responses to cellular oxidative stress . Under the Individual exposure of PFOA, when the concentration was greater than 20.0 mg/L, the catalase (CAT) activity increased significantly, and when it was greater than 100.0 mg/L, the malondialdehyde (MDA) content increased significantly, but there is no significant change under combined exposure. PVC and PFOA exposure also caused physical damage to the algal cells and reduced the content of extracellular polymer substances (EPS) based on analysis of cell morphology. Metabolic analysis revealed that carbohydrate metabolism and amino acid metabolism of the algae were affected. The current study offers a fresh theoretical framework for MPs and PFASs environmental risk evaluations.
研究领域:
环境科学
·
毒理学
·
水生生态学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Xing Gao,Weiying Feng,Hanxiao Zhang,Nanyan Weng,Shouliang Huo
Harmful cyanobacterial blooms in eutrophic water bodies have frequently occurred worldwide and become a major environmental concern. Therefore, it is imperative to develop a stable and efficient algaecide to solve this issue. In this study, our purpose was to investigate the efficacy and mechanism of a newly developed Cu 2+ doped Fe 3 O 4 @Biochar magnetic composite (Cu-Mag-BC) in in-situ inactivation of Microcystis aeruginosa ( M. aeruginosa ) . We successfully synthesized the Cu-Mag-BC by coating Cu 2+ onto Fe 3 O 4 @Biochar. Cu-Mag-BC exhibited superparamagnetic behavior and was uniformly impregnated by Cu 2+ . Cu-Mag-BC (5 mg/L), rapidly inactivated chlorophyll-a (Chl-a) in M. aeruginosa with low Fe and Cu leaching, during which time the OD 264 value and malondialdehyde (MDA) content increased, while the activities of superoxide dismutase (SOD) and catalase (CAT) first increased and then decreased, due to oxidative stress induced by over-generated reactive oxygen species (ROS). Quantitative results showed that ·O 2 − and ·OH were the main ROS species produced from Cu-Mag-BC. Inactivation efficiency was maintained at approximately 80 % after three consecutive runs and total Chl-a removal efficiency reached 2.84 g/g, indicating good reusability and stability. A possible inactivation mechanism is proposed; amino groups and adipose chain were the primary oxidation sites. Thus, Cu-Mag-BC shows potential as a candidate for simultaneously inactivating harmful cyanobacteria and preventing secondary pollution.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2026
·
IF 3.7
Wenlong Liu,Jin Dang,Yanguang Zhang,Jing Tian,Weibing Li
The limited recovery rate of powdered photocatalysts and potential secondary pollution issues have constrained the practical application of photocatalytic technology. Therefore, it is urgent to develop easily recoverable photocatalysts to address harmful algal blooms. In this study, a simple sol-gel method was used to synthesize a recyclable TiO 2 /FeTiO 3 composite photocatalyst on ceramic membrane for photocatalytic inactivation of Microcystis aeruginosa . TiO 2 /FeTiO 3 photocatalysts exhibit stable algal removal performance under various conditions (natural organic matter content, alkalinity levels et al. ), the TiO 2 /FeTiO 3 -4 photocatalyst with the best performance can achieve 100% photocatalytic inactivation of Microcystis aeruginosa (OD 680 =0.8) within 4 h of light irradiation. TiO 2 /FeTiO 3 photocatalysts can not only inactivate Microcystis aeruginosa , but also degrade intracellular and extracellular organic matter. Free radical scavenging experiments prove that holes play a major role in the photocatalytic inactivation of Microcystis aeruginosa , additionally, cyclic stability tests indicate that the photocatalyst is easy to recover and maintains a degradation rate of 97.19% even after three cycles. The process of preparing recyclable photocatalysts on ceramic membranes provides new ideas for the application of photocatalytic technology in pollutant treatment.
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
·
IF 6.6
Jie Cheng,Zhenxia Zhu,Shuohan Wang,Shuxian Guo,Jiashun Li,Chengshuai Xu,Yanhanjin Wang,Chaobo Zhang,Kaidian Zhang
PLA inhibits the biomass accumulation of M. aeruginosa FACHB-915. • High PLA exposure impairs photosystem II photochemistry. • Electron transport at donor and acceptor side of PSII are disrupted. • PLA induces a selective antioxidant enzyme response. • Growth inhibition is accompanied by extracellular microcystins accumulation. Phenyllactic acid (PLA) has been reported as a bioactive organic acid with anticyanobacterial potential, but its direct ecotoxicological effects on toxin-producing Microcystis aeruginosa remain insufficiently understood. In this study, purified PLA was used to evaluate its effects on the growth, photosynthetic physiology, antioxidant enzyme response and microcystin distribution of M. aeruginosa FACHB-915. Results indicated that PLA inhibited algal growth in a concentration-dependent manner after 48 h exposure with an estimated IC 50 value of 158.3 mg·L −1 , and high PLA exposure markedly reduced photosynthetic pigment production. Meanwhile, fast chlorophyll a fluorescence analysis further showed that exposure to 150.0 mg·L −1 PLA markedly altered PSII coordination, donor-side electron supply, and acceptor-side electron transport in M. aeruginosa . In addition, 150.0 mg·L −1 PLA decreased total protein content and significantly increased SOD activity, whereas POD activity did not show a parallel increase, suggesting distinct responses between the two assessed antioxidant enzymes. Notably, PLA exposure altered microcystin partitioning, and extracellular microcystin concentration increased significantly from 108.01 ng·mL −1 in the control to 126.48 ng·mL −1 at 150.0 mg·L −1 PLA, representing an approximate increase of 17.1%. These findings demonstrate that the growth suppression under PLA exposure was accompanied by pronounced PSII photochemical disturbance and physiological stress, and extracellular microcystin accumulation was also simultaneously enhanced, highlighting the need for careful evaluation of PLA as a potential anticyanobacterial compound.
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-12
·
IF 3.8
Cheng Jie,Xu Chengshuai,Sun Yang,Yu Qiuhan,Ding Shuo,Wang Yucai,Wei Wenxue,Xu Wei,Zhang Chaobo,Gong Donghui
Water eutrophication has emerged as a pressing concern for massive algal blooms, and these harmful blooms can potentially generate harmful toxins, which can detrimentally impact the aquatic environment and human health. Consequently, it is imperative to identify a safe and efficient approach to combat algal blooms to safeguard the ecological safety of water. This study aimed to investigate the procedure for extracting total flavonoids from Z. bungeanum residue and assess its antioxidant properties. The most favorable parameters for extracting total flavonoids from Z. bungeanum residue were a liquid–solid ratio (LSR) of 20 mL/g, a solvent concentration of 60%, an extraction period of 55 min, and an ultrasonic temperature of 80 °C. Meanwhile, the photosynthetic inhibitory mechanism of Z. bungeanum residue extracts against M. aeruginosa was assessed with a particular focus on the concentration-dependent toxicity effect. Z. bungeanum residue extracts damaged the oxygen-evolving complex structure, influenced energy capture and distribution, and inhibited the electron transport of PSII in M. aeruginosa . Furthermore, the enhanced capacity for ROS detoxification enables treated cells to sustain their photosynthetic activity. The findings of this study hold considerable relevance for the ecological management community and offer potential avenues for the practical utilization of resources in controlling algal blooms.
研究领域:
环境科学
·
植物化学
·
水生生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Weicheng Tang,Jianqiong Mou,Yujuan Chen,Yunli Yu,Xiaoyang Yu,Qiaolian Cheng,Zihao Wan,Wenfeng Tan,Mingming Liu
The quality of microcystin-LR (MC-LR) standards is frequently low and varies considerably due to the quite complex and challenging isolation and purification of MC-LR from Microcystis aeruginosa lysates. To streamline the way of standard acquisition, we describe an approach that combines rational design and directed chemical evolution for discovering synthetic hydrogel copolymers, which are potential abiotic affinity materials for MC-LR. A small focused polymer library was engineered by incorporating various combinations and ratios of candidate N-acryloyl-(l)-amino acid monomers, including N-acryloyl-glycine (AGly), N-acryloyl-l-phenylalanine (APhe), and N-acryloyl-l-glutamic acid (AGlu), that were selected based on the analysis of structural and physicochemical properties of MC-LR. A hydrogel copolymer AGlu-NP13 (58% AGlu, 40% TBAm, and 2% BIS) that had a high and specific affinity to MC-LR was screened from the polymer library. The hydrogen bond interaction may dominate the AGlu-NP13–MC-LR binding. The external conditions that influence the hydrogen bond interaction, including pH and solvation environments, controlled the catch and release of MC-LR on AGlu-NP13. The uptake of MC-LR is maximum under a saturated ethyl acetate (10%, v/v) solution at pH 3. The bound MC-LR can be completely desorbed from the hydrogel copolymer with a pH 9.5 and 70% methanol aqueous solution. The screened polymer affinity ligand was successfully applied for the isolation and purification of MC-LR from the lyophilized cell extracts of M. aeruginosa 905, providing a potential application of this material in standard preparation, sample pretreatment, and adsorption removal of MC-LR in complicated environmental and biological samples.
研究领域:
环境科学
·
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2025
·
IF 8.4
Yaxin Guo,Zongcheng Yao,Xiating Zhao,Yuting Wang,Qiming Xian
The structural specificity of organic nitrogen sources in modulating cyanobacterial physiology and toxin production remains poorly understood. This study systematically evaluated the bioavailability of exogenous glycine peptides in Microcystis aeruginosa ( M. aeruginosa ) and their regulatory roles in algal growth and microcystins (MCs) synthesis through an integrated physiological and transcriptomic approach. Glycine peptides demonstrated hierarchical bioavailability: glycine (Gly) > Glycylglycine (Gly2) > Tetraglycylglycine (Gly4) > Hexaglycylglycine (Gly6) > Cycloglycylglycine (CGly2). Gly and Gly2 were rapidly assimilated by M. aeruginosa , supporting robust algal growth comparable to the control group with nitrate as nitrogen source, while CGly2 and Gly6 induced nitrogen stress, damaging biomass and photosynthetic pigments. Notably, M. aeruginosa cultured with Gly4 as sole nitrogen source uniquely enhanced intracellular MCs production, and shifted MCs variants toward the highly toxic MC-LR. The shift in MCs variants ratios (MC-RR/MC-LR) reflected nitrogen availability, demonstrating a trade-off between nitrogen storage and toxin potency. Transcriptomic profiling unveiled nitrogen-centric metabolic rewiring, resulting in enhanced nitrogen assimilation, mcy genes for MCs biosynthesis, photosynthetic efficiency, and resource reallocation. While exogenous glycine peptides represent only a minor component of organic nitrogen in natural systems, these findings provide mechanistic insights into how peptide structure can influence cyanobacterial physiology and toxin profiles, contributing to a more nuanced understanding of organic nitrogen utilization by harmful cyanobacteria.
研究领域:
环境微生物学
·
水生生态学
·
毒理学
·
分子生物学
·
藻类学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Heshan Zheng,Yongjie Zheng,Le Yuan,Shuo Li,Junfeng Niu,Xu Dong,Yoong Kit Leong,Duu-Jong Lee,Jo-Shu Chang
The study investigated the inactivation of Microcystis aeruginosa using a combined approach involving thermally activated peroxyacetic acid (Heat/PAA) and thermally activated persulfate (Heat/PDS). The Heat/PDS algal inactivation process conforms to first-order reaction kinetics. Both hydroxyl radical (•OH) and sulfate radical (SO 4 - •) significantly impact the disruption of cell integrity, with SO 4 - • assuming a predominant role. PAA appears to activate organic radicals (RO•), hydroxyl (•OH), and a minimal amount of singlet oxygen ( 1 O 2 ). A thorough analysis underscores persulfate's superior ability to disrupt algal cell membranes. Additionally, SO 4 - • can convert small-molecule proteins into aromatic hydrocarbons, accelerating cell lysis. PAA can accelerate cell death by diffusing into the cell membrane and triggering advanced oxidative reactions within the cell. This study validates the effectiveness of the thermally activated persulfate process and the thermally activated peroxyacetic acid as strategies for algae inactivation.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Gongduan Fan,Banghao Du,Jinjin Zhou,Zhongsen Yan,Yifan You,Jing Luo
The practical application of photocatalytic technology in the natural water body is limited by the low reuse capability and potential secondary pollution risk of powder catalysts. Consequently, it is highly desirable to develop the stable floating photocatalytic composites for efficient alleviating harmful algal blooms. In this study, the self-floating Ag 2 O/g-C 3 N 4 /hydrogel composites with a porous three-dimension (3D) network nanostructure via the cross-linking of chitosan and polyvinyl alcohol have been synthesized. The effects of doping load, initial algal density and dissolved organic matter on Microcystis aeruginosa ( M . aeruginosa ) removal were investigated by Ag 2 O/g-C 3 N 4 /hydrogel under visible light irradiation. Among the synthesized photocatalysts, the 20-Ag 2 O/g-C 3 N 4 /hydrogel showed the highest adsorption, good reusability performance and excellent photocatalytic activity within 4 h for the removal of M . aeruginosa . Algal cell membrane and phycobiliprotein were severely damaged during the photocatalytic process. The inactivation mechanisms are attributed to the water-adsorbing swelling of the hydrogel and the generated reactive oxygen species (mainly ·O 2 − and ·OH) by Ag 2 O/g-C 3 N 4 . Moreover, the antioxidant system of the algae cells was also collapsed under the effects of reactive oxygen species. The superoxide dismutase (SOD) and catalase (CAT) content of algal cells decreased rapidly after photocatalytic reaction by Ag 2 O/g-C 3 N 4 /hydrogel, while the malondialdehyde (MDA) activity was higher than that of the control group. The self-floating Ag 2 O/g-C 3 N 4 /hydrogel photocatalyst would have real practical applications for harmful algae in the fields of natural water body.
研究领域:
环境科学
·
微生物学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）