IONICS
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2015
·Vol. 21
·期 10
·2911-2917
·
IF 1.754
Zhu Shuyun,Zhao Xian-en,Chen Guang,Wang Hua,Xu Guobao,You Jinmao
We report a simple method for the direct and quantitative determination of dihydronicotinamide adenine dinucleotide (NADH) using a single-walled carbon nanohorn (SWCNH) modified glassy carbon electrode (GCE). The electrochemically activated SWCNH modified GCE (SWCNH/GCE) substantially lowers the overpotential necessary for NADH oxidation compared to the inactivated SWCNH/GCE or bare GCE. We observe a 89-mV shift in the peak potential of NADH from GCE to SWCNH/GCE and another 101-mV shift from inactivated SWCNH/GCE to activated SWCNH/GCE in phosphate buffer (pH 7.0) at a scan rate of 50 mV/s. The activated SWCNH/GCE shows a linear response toward NADH between 1 and 100 μM with the detection limit of 0.2 μM. The activated SWCNH/GCE displays good reproducibility, high sensitivity, and excellent stability. Furthermore, the fabricated electrochemical sensors were used to detect NADH in serum by standard addition method and the recoveries are satisfactory.
研究领域:
分析化学
·
生物化学
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DOI
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2025
·
IF 2.5
Yao Zhang,Shiming Zhang,Li Luo,Sansan Hu,Xujuan Huang,Yongzheng Chen
Using visible light to drive NADH regeneration is an economically viable and environmentally sustainable technique. However, it necessitates a metal hydride (MH, [CpRh(bpy)(H 2 O)] 2+ ) as a synergist, and the high cost of the Rh noble metal significantly impedes the development and application of in-situ NADH regeneration. Therefore, in this study, single-atom Rh was immobilized onto the CdS QDs@SiO 2 combination via a consecutive ball-milling technique in combination with ionic layer adsorption and substitution. Subsequently, an enhanced photo-metal synergistic catalysis system for the recyclable regeneration of NADH was developed. In this composite, the single-atom Rh serves two main functions: It acts as an electrical medium and a metal catalyst, which regulates the activity and selectivity of the regenerated NADH. This study has successfully addressed the key scientific issues regarding the low electron transport rate and the recycling of the Rh noble metal during catalysis. Results confirm that single-atom Rh is successfully immobilized onto the CdS QDs@SiO 2 combination (Rh-CdS@SiO 2 ) and exhibits a faster electron transport and enhanced selectivity. Under blue light (LED, 420 nm) irradiation, the Rh-CdS@SiO 2 photo-metal catalyst shows a 25-fold increase in recyclable operability and achieves a 68% regeneration yield of NADH in just 4 min. Moreover, (S)-(+)-4-phenyl-2-butanol can be obtained with the regenerated NADH as the coenzyme of P450 enzyme catalysis.
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ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Yin Hua Cui,Qing Shan Shi,Dan Dan Zhang,Ling Ling Wang,Jin Feng,Yi Wen Chen,Xiao Bao Xie
Ionic liquids (ILs), also known as green solvents, are widely acknowledged in several fields, such as chemical separation, synthesis, and electrochemistry , owing to their excellent physiochemical properties. However, their poor biodegradability may lead to environmental and health risks, posing a severe threat to humans, thus requiring further research. In this study, the biotoxicities of the imidazolium-based ILs were evaluated in Tetrahymena pyriformis . Moreover, IL detoxification was investigated by addition of glutathione (GSH), cysteine, and nicotinamide adenine dinucleotide (NADH). Reactive oxygen species (ROS) initiated by different IL types caused damage to Tetrahymena , while glutathione, cysteine, and NADH eliminated ROS, achieving the detoxification purposes. Detoxification results showed that NADH exhibited the best detoxification ability, followed by glutathione and cysteine. Finally, RT-PCR results suggested that metallothionein might have participated in IL detoxification.
研究领域:
环境科学
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毒理学
·
生物化学
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DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Lu Zhao,Wenhe Zhang,Qi Wang,Huibin Wang,Xiao Gao,Bin Qin,Xian Jia,Song You
L -Phosphinothricin ( L -PPT) is the effective constituent in racemic PPT (a high-efficiency and broad-spectrum herbicide), and the exploitation of green and sustainable synthesis route for L -PPT has always been the focus in pesticide industry. In recent years, “one-pot, two-step” enzyme-mediated cascade strategy is a mainstream pathway to obtain L -PPT. Herein, Rg DAAO and Bs LeuDH were applied to expand “one-pot, two-step” process. Notably, a NADH-dependent leucine dehydrogenase from Bacillus subtilis ( Bs LeuDH) was firstly characterized and attempted to generate L -PPT, achieving an excellent enantioselectivity (99.9% ee ). Meanwhile, a formate dehydrogenase from Pichia pastoris ( Pp FDH) was utilized to implement NADH cofactor regeneration and only CO 2 was by-product. Sufficient amount of the corresponding keto acid precursor PPO was obtained by oxidation of D -PPT relying on a D -amino acid oxidase from Rhodotorula gracilis ( Rg DAAO) with content conversion (46.1%). L -PPT was ultimately prepared from racemized PPT via oxidative deamination catalyzed by Rg DAAO and reductive amination catalyzed by Bs LeuDH, achieving 80.3% overall yield and > 99.9% ee value.
研究领域:
生物化学
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农药合成
·
酶工程
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DOI
PubMed
高影响因子
ACS Catalysis
·
2025
·
IF 13.1
Shihao Li,Jiafu Shi,Yu Chen,Xinyu Mao,Shusong Liu,Yang Yang,Hong Wu,Zhongyi Jiang
Amino alcohols are widely used in chemical and pharmaceutical industries, while conventional synthesis methods face drawbacks such as low selectivity and high energy consumption. Multienzyme catalysis combined with light-driven cofactor regeneration affords a platform of light-driven synthesis of chemicals, where the transfer of electrons and protons as well as the route of enzymatic reactions dominates the energy conversion efficiency and atomic economy of carbon utilization. Herein, we construct a light-driven synthesis system comprising an energy-supplying module and a multienzyme catalytic module for 2-aminoethanol synthesis from formaldehyde and ammonia. First, a S-scheme heterojunction photocatalysts with a metal–organic framework core and a bipyridine-based conjugated polymer shell are prepared as an energy-supplying module. Through synergistic intensification of proton transfer and electron transfer, the photocatalysts render cofactor (NADH) regeneration efficiency with a turnover frequency of 30.49 h–1. Second, a multienzyme catalytic module is constructed for 2-aminoethanol synthesis in the presence of NADH. Under optimal conditions, this module produces 2-aminoethanol with an average reaction rate of 76.34 μmol L–1 h–1 within 24 h. Finally, the energy-supplying module is coupled with the multienzyme catalytic module for continuous synthesis of 2-aminoethanol from formaldehyde and ammonia, generating 0.55 mmol L–1 2-aminoethanol after 4 light–dark cycles. In addition, triethanolamine (TEOA) and glucose, a biomass-derived carbon source, are both confirmed as effective electron donors for NADH regeneration and light-driven synthesis system. This work opens a pathway for the synthesis of amino alcohols and demonstrates a paradigm for the intensification of reaction processes.
研究领域:
生物化学
·
绿色化学
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DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 8
·1716-1721
·
IF 5.227
Xiaobin Wang,Yuqing Zhao,Qing Hua,Jiaojiao Lu,Feiyan Tang,Wenjie Sun,Feng Luan,Xuming Zhuang,Chunyuan Tian
In this paper, the electrochemiluminescence (ECL) of sulfur quantum dots (SQDs) in a potassium persulfate cathodic co-reactant was studied. Based on the selective quenching of the ECL emission from the SQDs by β-nicotinamide adenine dinucleotide (NADH), an ultrasensitive ECL biosensor with NADH as an important parameter was established for the highly sensitive and selective detection of total bacterial count (TBC). A linear response between the ECL intensity and the NADH concentration in the range of 1 pM to 10 μM was obtained, thus achieving a detection limit of 1 pM. As the content of NADH in cells is positively correlated with the TBC, a sensor has been successfully applied to detect the TBC in actual water samples with a good recovery rate of 103–107 CFU mL−1. This study provides a green and feasible method for TBC detection in the environment.
研究领域:
环境科学
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分析化学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2018
·
IF 4.019
Chen Xin,Tang Hongzhi,Liu Yongdi,Xu Ping,Xue Yong,Lin Kuangfei,Cui Changzheng
3-Hydroxybenzoate 6-hydroxylase, an NADH-dependent flavoprotein, can convert 3-hydroxybenzoate which is an important intermediate in the biodegradation of many aromatic hydrocarbons. 3-Hydroxybenzoate is metabolized by entering the TCA cycle through the gentisate pathway. We found a putative 3HB6H gene from a cluster that potentially encodes for gentisic acid degradation from a halophilic Martelella sp. strain AD-3. The corresponding protein was expressed with an N-terminal His-tag and purified by Ni2+-nitrilotriacetic acid affinity chromatography. The protein showed an overexpressed band of about 46 kDa by SDS–PAGE, and it was also proven that the enzyme contains FAD by absorption spectroscopy and HPLC analysis. The optimal activity of 3HB6H from strain AD-3 was observed in phosphate buffer (pH 8.0) at 37∘C without salinity (NaCl) and metal salts. The Km values of 3-hydroxybenzoate 6-hydroxylase were determined to be 72.6 ± 10.1 μM and 104.1 ± 18.2 μM for 400 μM NADH and 3-hydroxybenzoate, respectively. Site-directed mutagenesis showed that residues 305, 306 and 308 are important for FAD binding. In addition, we found that Tyr221 and Gln305 of 3HB6H from strain AD-3 are involved in substrate binding.
研究领域:
微生物学
·
酶学
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2018
·Vol. 8
·期 2
·127
·
IF 3.504
Sen Lin,Shiyong Sun,Ke Wang,Kexuan Shen,Biaobiao Ma,Yuquan Ren,Xiaoyu Fan
The bioinspired design and construction of enzyme@capsule microreactors with specific cell-like functionality has generated tremendous interest in recent years. Inspired by their fascinating complexity, scientists have endeavored to understand the essential aspects of a natural cell and create biomimicking microreactors so as to immobilize enzymes within the hierarchical structure of a microcapsule. In this study, simultaneous encapsulation of alcohol dehydrogenase (ADH) was achieved during the preparation of microcapsules by the Pickering emulsion method using amphiphilic modified TiO2nanoparticles (NPs) as building blocks for assembling the photocatalytic microcapsule membrane. The ADH@TiO2NP microreactors exhibited dual catalytic functions, i.e., spatially confined enzymatic catalysis and the membrane-associated photocatalytic oxidation under visible light. The sustainable cycling of nicotinamide adenine dinucleotide (NAD) coenzyme between NADH and NAD+was realized by enzymatic regeneration of NADH from NAD+reduction, and was provided in a form that enabled further photocatalytic oxidation to NAD+under visible light. This bioinspired ADH@TiO2NP microreactor allowed the linking of a semiconductor mineral-based inorganic photosystem to enzymatic reactions. This is a first step toward the realization of sustainable biological cycling of NAD+/NADH coenzyme in synthetic functional microsystems operating under visible light irradiation.Keywords:enzyme encapsulation;bioinspired microreactors;nicotinamide coenzymes cycling;alcohol dehydrogenase;TiO2nanoparticles
研究领域:
生物化学
·
材料科学
·
光催化
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DOI
PubMed
高影响因子
AICHE JOURNAL
·
2026
·
IF 4
Shihao Li,Jiafu Shi,Yu Chen,Hong Wu,Zhongyi Jiang
In photocatalytic hydrogenation, non-directional transfer of electrons and indiscriminate oxidation of products by holes often result in severe electron–hole recombination and unintended product degradation, limiting overall efficiency. Herein, we engineer both the substrate reduction and product oxidation pathways through an interface–pore co-confinement strategy, exemplified by a rationally designed covalent organic framework (COF)-bridged core–shell photocatalyst (U@TpBpy-Rh) and photocatalytic NADH synthesis. The resultant U@TpBpy-Rh functions through dual mechanisms: (1) the confined S-scheme heterojunction interface facilitates directional electron transfer from MOF core to Rh sites in COF, significantly enhancing the photocatalytic NADH synthesis with productivity of 2.82 mmol g −1 h −1 ; (2) the confined pores of the COF shell restrict NADH diffusion toward highly oxidative MOF regions, reducing its oxidation degradation by 63% compared to U@TpBpy. This work demonstrates simultaneous spatiotemporal management of competitive photocatalytic reduction and oxidation pathways and will offer a general strategy for optimizing other redox-coupled reactions.
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DOI
高影响因子
Materials Chemistry Frontiers
·
2026
·
IF 8.1
Xuetong Cai,Chi Meng,Yajie Zhang,Fude Feng
Artificial NADH oxidase (NOX) has distinct advantages in cost, stability and manufacture as compared to the natural oxidases. Methylene blue is a redox-active phenothiazine derivative with weak NOX-like activity in mediating electron transfer. Herein, we applied a one-pot iron-catalyzed oxidative polymerization method to prepare a methylene blue backboned polymer (MBPN-10) with pendant quaternary ammonium groups and compared with methylene blue in the catalytic activity of NADH oxidation. MBPN-10 showed increased NADH binding affinity, improved catalytic performance, and was compatible with long-term on-shelf storage and organic solvents. The catalytic reaction by MBPN-10 is efficient to afford H2O2 without the need of metals or light. These features make MBPN-10 a highly promising synthetic NOX mimic.
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高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2026
·
IF 3.1
Pan Jianzhen,Liu Xinting,Yang Minghui
By in situ reduction of Pt nanoparticles (Pt NPs) onto the surface of multi-walled carbon nanotubes (MWCNTs), Pt NPs@MWCNTs nanocomposite was successfully synthesized. Experimental results indicate the nanocomposite exhibits good NADH oxidase activity, which can generate •O 2− through O 2 and efficiently catalyze the oxidation of nicotinamide adenine dinucleotide (NADH) to generate biologically active NAD + . Based on this characteristic, we developed an NAD + /NADH dependent dehydrogenase electrochemical cascade biosensor for the detection of β-hydroxybutyric acid (HB) via β-hydroxybutyrate dehydrogenase that does not require additional redox mediators. This biosensor exhibited good performance for HB with high sensitivity and good specificity, and a wide linear range of 10 µM to 10 mM. To verify its practical application, the biosensor was applied to the detection of HB in human serum samples, which indicate the biosensor has potential for clinical diagnosis of diabetes ketoacidosis.
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高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2019
·
IF 3.553
Fei-Long Li,Wen Bin Su,Qing-Lan Tao,Liao-Yuan Zhang,Ye-Wang Zhang
Enzyme-catalyzed cofactor regeneration is a significant approach to avoid large quantities consumption of oxidized cofactor, which is vital in a variety of bioconversion reactions. NADH: FMN oxidoreductase is an ideal regenerating enzyme because innocuous molecular oxygen is required as an oxidant. But the by-product H 2 O 2 limits its further applications at the industrial scale. Here, novel NADH: FMN oxidoreductase (LrFOR) from Lactobacillus rhamnosus comprised of 1146 bp with a predicted molecular weight of 42 kDa was cloned and overexpressed in Escherichia coli BL21 (DE3). Enzyme assay shows that the purified recombinant LrFOR has both the NADPH and NADH oxidation activity. Biochemical characterizations suggested that LrFOR exhibits the specific activity of 39.8 U·mg −1 with the optimal pH and temperature of 5.6 and 35 °C and produces H 2 O instead of potentially harmful peroxide. To further study its catalytic function, a critical Thr29 residue and its six mutants were investigated. Mutants T29G, T29A, and T29D show notable enhancement in activities compared with the wild type. Molecular docking of NADH into wild type and its mutants reveal that a small size or electronegative of residue in position29 could shorten the distance of NADH and FMN, promoting the electrons transfer and resulting in the increased activity. This work reveals the pivotal role of position 29 in the catalytic function of LrFOR and provides effective catalysts in NAD + regeneration.
研究领域:
生物化学
·
酶学
·
微生物学
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DOI
PubMed
高影响因子
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