JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Menghua Dou,Siwei Wang,Wei Li,Qianjin Li,Jianhong Xu,Jianlin Li
Development of selective enrichment materials for the accurate analysis of ochratoxin a (OTA) in environmental and food samples is an effective way to protect human health. Here, a molecularly imprinted polymer (MIP) known as plastic antibody was synthesized onto the magnetic inverse opal photonic crystal microsphere (MIPCM) using a low-cost dummy template imprinting strategy targeting OTA. The MIP@MIPCM exhibited ultrahigh selectivity with an imprinting factor of 130, high specificity with cross-reactivity factors of 3.3–10.5, and large adsorption capacity of 60.5 μg/mg. Such MIP@MIPCM was used for selective capture of OTA in real samples which was quantified in combination with high-performance liquid chromatography, giving a wide linear detection range of 5–20,000 ng/mL, a detection limit of 0.675 ng/mL, and good recovery rates of 84–116%. Moreover, the MIP@MIPCM can be produced simply and rapidly and is very stable under different environmental conditions and easy to store and transport, so it is an ideal substitute of biological antibody modified materials for the selective enrichment of OTA in real samples.
研究领域:
分析化学
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食品安全
·
材料科学
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DOI
PubMed
高影响因子
MACROMOLECULAR RESEARCH
·
2022
·Vol. 30
·期 10
·719-730
·
IF 2.127
Zhang Xing,He Juan,Wang Huige,Xu Pengfei,Wang Mingyu,Li Yuanyuan,Chen Jing,He Lijun
In this study, surface molecularly imprinted polymers were synthesized with the metal-organic framework NH 2 -MIL-53 as carrier and naringenin as alternative template of Ochratoxin A (OTA). The amino groups on the surface of NH 2 -MIL-53 reacted with the epoxy groups in glycidyl methacrylate (GMA). Subsequently, the double bonds of the GMA grafting on the surface of the material were used for polymerization to prepare the surface molecularly imprinted polymers. The obtained polymers were characterized by scanning electron microscope, transmission electron microscope, energy spectrometer, Fourier infrared spectrometer, X-ray diffraction, and laser particle size analyzer. Synthetic polymer was combined with solid phase extraction for separation and enrichment of trace OTA in grains. The spiked recoveries were found to be 78.95–106.45%, with relative standard deviations ranging from 5.16% to 6.31%. The detection limit and the quantification limit of the method were 0.04 µg·kg −1 and 0.12 µg·kg −1 , respectively. Comparing the pretreatment effects of the self-made cartridge and the immunoaffinity column on the OTA in the sample, it showed that the self-made column had excellent performance and provided a new pretreatment method for the treatment of OTA in grains.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 45
·455501
·
IF 3.874
Hao Huang,Dan Wang,Ying Zhou,Dongping Wu,Xiaoning Liao,Wanming Xiong,Juan Du,Yanping Hong
Electrochemical sensor has great potential in the detection of small molecules by virtues of low cost, fast response, and easy to miniaturization. However, electrochemical sensing of ochratoxin A (OTA) was seriously hindered by the heavy electrode-fouling effect and poor electrochemical activity inherent from OTA molecular. Herein, two-dimensional titanium carbide (2D Ti3C2) MXene incorporated with carboxylic multiwalled carbon nanotubes (cMWCNTs) was developed as a glassy carbon electrode modifier for rapid and sensitive detection of OTA. Physical characterizations combined with electrochemical techniques revealed that cMWCNTs can not only prevent the restacking of 2D Ti3C2 nanosheets but also facile its electron transfer, leading to a nanohybrid with a high specific surface and good electrocatalytic activity to OTA. Under optimal conditions, the electrochemical sensor showed a good linear response to OTA in a concentration range from 0.09 to 10 μmol·l−1 and a low detection limit (LOD) of 0.028 μmol·l−1. The proposed sensor was impelled successive times to detect OTA, a good repeatability was obtained, indicating the constructed sensor possessed good anti-fouling property. Moreover, satisfactory recoveries between 91.8% and 103.2% were obtained in the real sample analysis of grape and beer, showing that the developed sensing technique is reliable for the screening of trace OTA in food resources.
研究领域:
食品科学
·
分析化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Zongwen Tang,Xing Liu,Benchao Su,Qi Chen,Hongmei Cao,Yonghuan Yun,Yang Xu,Bruce D. Hammock
Ochratoxin A (OTA) is a major concern for public health and the rapid detection of trace OTA in food is always a challenge. To minimize OTA exposure to consumers, a nanobody (Nb)-mediated förster resonance energy transfer (FRET)-based immunosensor using quantum dots (Nb-FRET immunosensor) was proposed for ultrasensitive, single-step and competitive detection of OTA in agro-products at present work. QDs of two sizes were covalently labeled with OTA and Nb, acting as the energy donor and acceptor, respectively. The free OTA competed with the donor to bind to acceptor, thus the FRET efficiency increased with the decrease of OTA concentration. The single-step assay could be finished in 5 min with a limit of detection of 5 pg/mL, which was attributed to the small size of Nb for shortening the effective FRET distance and improving the FRET efficiency. The Nb-FRET immunosensor exhibited high selectivity for OTA. Moreover, acceptable accuracy and precision were obtained in the analysis of cereals and confirmed by the liquid chromatography-tandem mass spectrometry. Thus the developed Nb-FRET immunosensor was demonstrated to be an efficient tool for ultrasensitive and rapid detection of OTA in cereals and provides a detection model for other toxic small molecules in food and environment.
研究领域:
食品安全
·
生物技术
·
分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 3
·1-10
·
IF 5.833
Zhu Hongshuai,Liu Chuanhe,Liu Xinxin,Quan Zhu,Liu Weipeng,Liu Yingju
A multi-colorimetric immunosensor basing on the mimetic enzyme etching of gold nanobipyramids (Au NBPs) was established to detect ochratoxin A (OTA). Octahedral Cu 2 O nanoparticles were successfully synthesized through a selective surface stabilization strategy, which can exhibit a peroxidase-like ability to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB). Au NBPs can be etched by the product, TMB 2+ , to form a significant longitudinal peak blue shift of local surface plasmon resonance. During the construction of the immunosensor, the microplate was coated with dopamine to immobilized OTA antigens, followed by the immunoreaction of OTA antibody and the Cu 2 O-labled secondary antibody. A linear relationship can be found between the local surface plasmon resonance (LSPR) peak changes with the logarithm of OTA concentration in a wide range from 1 ng/L to 5 μg/L, while the detection limit was 0.47 ng/L. Meanwhile, the approximate OTA concentration can be conveniently and intuitively observed by the vivid color changes. Benefiting from the high specificity, the proposed multi-colorimetric immunoassay detection of OTA in millet samples was achieved, indicating the available potential of the immunoassay for the determination of OTA in real samples. Graphical abstract
研究领域:
分析化学
·
纳米技术
·
食品科学
·
毒理学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Hairong Guo,Yanan Zhao,Shanghong Ji,Tingtiao Pan,Weizhen Xu,Xumei Zhou,Yu Zhou
Eu-CP self-assembled at room temperature enables reverse regulation of dual fluorescence signals. • OTA LOD as low as 0.0273 ng/mL (0.4883–62.5 ng/mL linear range) with excellent sensitivity. • High specificity, good probe and inter-batch stability. • Qualified grain sample recovery, suitable for on-site primary screening. Ochratoxin A (OTA) contamination severely threatens global food safety. Herein, a ratiometric fluorescent immunoassay method was established for trace OTA detection based on an alkaline phosphatase (ALP)-catalyzed Eu 3+ coordination polymer (Eu-CP). The Eu-CP probe is self-assembled from ciprofloxacin, adenosine monophosphate, and Eu 3+ . Upon activation by the Mg 2+ cofactor, ALP drives the dephosphorylation and subsequent structural disassembly of the Eu-CP framework, which synchronously regulates dual-emission fluorescent signals. In indirect competitive reaction, OTA competes with bovine serum albumin-OTA for limited antibodies, and the bound ALP-labeled secondary antibody is inversely correlated with OTA concentration. Based on the ratiometric fluorescence signal (F 618 /F 418 ), the proposed method exhibited a reliable linear range of 0.4883–62.5 ng/mL (R 2 = 0.9909) and a limit of detection as low as 0.0273 ng/mL. Recoveries in corn, oat and rice were 97.07%–119.11% with inter-batch CV < 10%. This study provides a simple and sensitive method for trace OTA detection. Download: Download high-res image (194KB) Download: Download full-size image
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DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Maosheng Sang,Yang Han,Heng Zhang,Zhongyu Li,Quanlong Zhou,Xiaoya Meng,Yuhao Zhang,Yemin Guo,Xia Sun,Surayo Rakhmatzhanovna Usmanova,Jamshed Bobokalonov,Wenping Zhao
An “on–off” electrochemiluminescence (ECL) aptasensor was proposed for the highly sensitive and selective detection of ochratoxin A (OTA) in cereals. The substrate nanomaterial employed in the ECL experiments was titanium carbide (Ti3C2Tx) MXene, while cadmium sulfide quantum dots (CdS QDs) were used as coreactants of the ECL reagent tris (2,2′-bipyridyl) ruthenium(II) (Ru(bpy)32+). Compared to tripropylamine, a more toxic conventional coreactant, CdS QDs can react efficiently with Ru(bpy)32+ in the ECL reaction, and the reaction principles of both were also investigated. The effective connection between CdS QDs and Ru(bpy)32+ via the aptamer resulted in a shortened electron transfer distance, which in turn led to an improvement in ECL intensity and the achievement of the first “on” state. The introduction of OTA resulted in the loss of the effective connection between the CdS QDs and Ru(bpy)32+, which caused the sensor to enter an “off” state. The high ECL intensity of the sensor in the “on” state, in contrast with the low ECL intensity of the “off” state, resulted in an increase in the sensitivity and stability of the sensor. The established aptasensor exhibited a low limit of detection (LOD) of 0.03 ng/mL within a linear range of 0.1 ∼ 100 ng/mL, with spiked recoveries ranging from 93.7% to 105.1%, showing good selectivity, stability, and reproducibility for the detection of OTA in cereals.
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Yihao Li,Yulu Cao,Junjie Huang,Jiachen Zhang,Yonghong Wang,Yuanqing Wang,Ge Ning
Ochratoxin A (OTA), a fungal toxin, induces various toxic effects in animals and humans through the enrichment of toxin residues. In this work, a dual-modal biosensor based on gold nanoparticles (AuNPs) and branched hybridization chain reaction (bHCR) was proposed for the detection of OTA. The strategy is contingent on OTA aptamer-bridged occurrence of bHCR and the salt-induced aggregation of AuNPs. OTA-apt/cDNA could be used to specifically identify the OTA and trigger bHCR reactions, producing a long-branched dsDNA polymer. The electroactive molecule-methylene blue (MB) can be inserted into the superstructure of branched DNA due to the formation of DNA polymers, leading to dynamic changes in MB redox signaling. The residual DNA hairpins were added and adhered to the surface of AuNPs, but they were inadequate to prevent the AuNPs from salt-induced aggregation. The dual-modal yields limits of detection of 4.8 pM (electrochemical assay) and 0.25 nM (colorimetric assay), respectively. It exhibited excellent specificity against common mycotoxins (AFB1, DON, FB1, ZEN), with satisfactory recoveries in corn flour (92.9–108.3 %). This aptasensor, which adopts a dual-modal strategy, features self-calibration to reduce false-positive results and improve accuracy. It demonstrates significant advantages in mycotoxin detection.
研究领域:
分析化学
·
食品安全
·
生物传感
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-9
·
IF 5.3
Yang Wei,Ni Lanxiu,Zhu Mingzhen,Zhang Xiaobo,Feng Liang
It has been observed that polyvalent metal ions can mediate the adsorption of DNA on polydopamine (PDA) surfaces. Exploiting this, we used two divalent metal ions (Mg 2+ or Ca 2+ ) to promote the adsorption of fluorescence-labelled ochratoxin A (OTA) aptamers on PDA-coated magnetic nanoparticles (Fe 3 O 4 @PDA). Based on the different adsorption affinities of free aptamers and OTA-bound aptamers, a facile assay method was established for OTA detection. The aptamers adsorbed on Fe 3 O 4 @PDA were removed via simple magnetic separation, and the remaining aptamers in the supernatant exhibited a positive correlation with the OTA concentration. The concentrations of Mg 2+ and Ca 2+ were finely tuned to attain the optimal adsorption affinity and sensitivity for OTA detection. In addition, other factors, including the Fe 3 O 4 @PDA dosage, pH, mixing order, and incubation time, were studied. Finally, under optimized conditions, a detection limit (3σ/s) of 1.26 ng/mL was achieved for OTA. Real samples of spiked red wine were analysed with this aptamer-based method. This is the first report of regulating aptamer adsorption on the PDA surface with polyvalent metal ions for OTA detection. By changing the aptamers, the method can be easily extended to other target analytes. Graphical abstract
研究领域:
分析化学
·
食品科学
·
生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 6
·H182
·
IF 3.12
Jing Zhang,Ke Yang,Lu Chen
This work proposed a novel and facile strategy for in-situ deposition of silver nanoparticles (AgNPs) on polydopamine nanospheres (PDANSs), and used the as-synthesized nanocomposite (AgNPs/PDANSs) as electrochemical tracer for an ultrasensitive aptasensor of ochratoxin A (OTA). The morphology and composition of PDANSs and AgNPs/PDANSs were characterized by electron microscopies and energy dispersive X-ray spectroscopy, and the results showed that AgNPs uniformly on PDANSs surface. AgNPs/PDANSs were combined with streptavidin (SA) to obtain signal probe for constructing of OTA aptasensor. Sequentially, the thiolated capture DNA (cDNA) was firstly immobilized on the gold electrode (GE) to capture the OTA aptamer, and then SA/AgNPs/PDANSs nanoprobes could bind with the end biotin of the aptamer through biotin-streptavidin biorecognition. The monitoring of the proposed OTA aptasensor was realized by the electrochemical stripping signal of AgNPs on PDANSs, and PDANSs was used for signal amplification. In the presence of OTA, the aptamer was preferentially bonded with OTA because of their high affinity, and resulted in the decrease of signal tags on the aptasensor surface, which reduced the electrochemical response signal of AgNPs. Under optimal experimental conditions, the proposed aptasensor offered a wide linear range from 1.0 × 10−6 μM to 1.0 × 10−2 μM and a low detection limit of 0.57 pM (S/N = 3). Meanwhile, This AgNPs/PDANSs-based OTA aptasensor had excellent specificity, stability and reproducibility, and was successfully employed for OTA detection in red wine samples, which showed promising application in electrochemical analysis of other toxic molecules.
研究领域:
分析化学
·
食品科学
·
毒理学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Ya-Juan Yang,Ying Zhou,Yang Xing,Guo-Mei Zhang,Yan Zhang,Cai-Hong Zhang,Peng Lei,Chuan Dong,Xu Deng,Yujian He,Shao-min Shuang
In this study, a novel aptasensor based on Aptamer/NH 2 Janus particles is developed for the detection of Ochratoxin A(OTA). By coating gold on the hemispherical surface of the aminated polystyrene particles, Ochratoxin A aptamer is immobilized on the surface of the gold layer for selective identification and the other hemispherical able to bind to Glassy carbon electrode via peptide bond. Under optimum conditions, the sensor exhibited a wide dynamic range of OTA concentration from 1 × 10 −5 nM to 10 nM, and the detection limit is 3.3 × 10 −3 pM on condition of acceptable stability and reproducibility. The sensors were showed excellent performance in the detection of OTA in red wine sample with recoveries between 95.7% and 100.18%, which studied by the standard addition spiking technique. This work provides a new idea and method for preparing immune electrochemical sensors and is expected to be used for the OTA detection in red wine sample analysis.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-7
·
IF 5.705
Liu Yufei,Yan Huijuan,Shangguan Jingfang,Yang Xue,Wang Meili,Liu Wei
A fluorometric aptamer-based assay for ochratoxin A (OTA) is described. It is making use of magnetic separation and a cationic conjugated fluorescent polymer. Amino-tagged aptamer (Apt) against OTA is immobilized on magnetic beads (MBs) to form a conjugate of type Apt-MBs. The immobilized aptamer is partially complementary to carboxyfluorescein-labeled DNA which binds to the Apt-MBs via hybridization if OTA is absent. Only few FAM-DNA will remain in the supernatant after magnetic separation, and only weak fluorescence resonance energy transfer (FRET) occurs on addition of the fluorescent polymer. If, however, OTA is present, it will bind to the aptamer and prevent the hybridization between Apt-DNA and FAM-DNA. This results in the presence of large amounts of FAM-DNA in the supernatant after magnetic separation. On addition of fluorescent polymer, efficient FRET occurs from the polymer to FAM-DNA. Fluorescence, best measured at excitation/emission peaks of 370/530 nm, increases with increasing concentrations of OTA. This assay is highly sensitive and selective. The detection limit is as low as 0.11 ng mL −1 . This is 6 times lower than the aptamer assay without using the fluorescent polymer. Conceivably, this method has a wider scope in that it may be extended to other mycotoxins by simply changing the aptamer.
Schematic of a fluorometric aptamer assay for ochratoxin A (OTA). It is based on magnetic separation coupled with a cationic conjugated polymer (PFP).
研究领域:
分析化学
·
毒理学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）