ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Congmin Liu,Xiaowen Dou,Lei Zhang,Weijun Kong,Liu Wu,Yaping Duan,Meihua Yang
In the present study, molecular modeling and principle component analysis (PCA) were used to select appropriate haptens for group detection of triazine herbicides. Four new structures together with three reported triazine derivatives were chosen for the screening of immunizing and coating haptens . A total of 31 triazines coupled with a 3D-QSAR methodology were employed to investigate the relationship between antigen-antibody recognition and molecular structures, the results of which revealed that the antibodies may recognize triazines from the side of molecules with the distinguishing atom and a steric volume matching with the spatial structure of antibodies. Finally, a broad-specificity heterologous immunoassay was developed for determining 10 triazine herbicides in ginger, where the detection limits were 2.5–15.1 μg kg −1 and recoveries were 67.9–102.6%. This study may broaden insight into triazine-antibody interactions and benefit designing novel performance-enhanced antibodies. The developed immunoassay can be further used for triazine detection in other complicated matrices.
研究领域:
分析化学
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分子生物学
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环境科学
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农业化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiao Zhou,Tianlin Wang,Minyang Zhao,Pengxiang Wang,Zhe Hao,Xiaodong Lin,Shuo Wang,Yaqing Liu
Rapid detection and identification of multiple metal ions is significant in that toxic metal ions are harmful to public health and environmental safety. Herein, a fluorescent sensor array composed of three lanthanide-based fluorescent probes (Ce-ATP, Tb-GMP, and Eu-BOP) was developed as a novel tongue-mimic chemosensor array for rapid discrimination of multiple metal ions. The three fluorescent probes exhibit different binding affinities toward metal ions, resulting in diverse fluorescence behaviors and distinct fluorescence response patterns. The patterns can act as “fingerprints” for identification of multiple metal ions via principal component analysis (PCA) technology. The Ce-ATP/Tb-GMP/Eu-BOP sensor array achieved good discrimination of eleven kinds of metal ions (Cu 2+ , Pb 2+ , Co 2+ , Zn 2+ , Cd 2+ , Mn 2+ , Fe 3+ , Cr 3+ , Ag + , Cr 6+ , Mn 7+ ). It is still sensitive in mixtures of multiple metal ions and would be potentially fit for analysis of complex composition. Interestingly, the sensor array can discriminate metal ions with different valence, for example Cr 3+ and Cr 6+ . With tap water as real sample, the eleven metal ions can be well distinguished with excellent recovery, indicating promising potential application in evaluating water quality and environmental protection.
研究领域:
分析化学
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环境科学
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纳米技术
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传感器
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 6
·3503-3514
·
IF 3.612
Ya-Xing Li,Chan Zhong,Hui-Zhen Zhang,Yuan-Yang Zhao,Mei Shu,Guo-Ping Wu
Summary A new gelatin-protocatechuic acid (PCA) film with Escherichia coli O157:H7 phage JN01 was developed and characterised. After incorporated with JN01, swelling value, water vapour permeability, water solubility and elongation at break of gelatin-PCA film were not significantly different. The addition of JN01 increased b value and transparency of film, while it decreased L value, a value and tensile strength of film. Moreover, the gelatin-PCA-JN01 film presented antioxidant activity of 60.07%. Furthermore, JN01 could be steadily released from gelatin-JN01 and gelatin-PCA-JN01 films in aqueous solution, and their release rates were 7.56% and 0.12% after 11 h, respectively. The microstructure analysis showed that JN01 particles were clustered and uniformly distributed in film, and the aggregation would be attenuated in the presence of PCA. Meanwhile, E . coli O157:H7 counts were 1.13 log 10 CFU mL −1 and 0.45 log 10 CFU mL −1 lower in gelatin-JN01 and gelatin-PCA-JN01 films compared with pure gelatin film in vitro at 4 °C for 24 h, respectively. After stored at 4 °C for 7 days, Escherichia coli O157:H7 counts were 1.00 log 10 CFU g −1 and 0.80 log 10 CFU g −1 lower in beef packed with these two gelatin films, compared with beef without gelatin film, respectively. In conclusion, the developed gelatin-PCA-JN01 film has potential application in food preservation.
研究领域:
食品科学
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微生物学
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生物技术
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3388
·
IF 5.561
Xijuan Tu,Yunmin Tao,Jiaxu Chen,Chunping Du,Qian Jin,Yuchang He,Ji Yang,Shaokang Huang,Wenbin Chen
In the present study, a lanthanide fluorescence sensor array was developed for the discrimination of honey’s botanical origin. Dipicolinic acid (DPA) was used as the antenna ligand for sensitizing the fluorescence of Tb3+and Eu3+to prepare the DPA-Tb3+/Eu3+complex. This lanthanide fluorescence sensor showed a cross-reactive response to the major constituents of honey, which led to the result that different classes of honey solution exhibited distinct quenching effects on the fluorescence of the DPA-Tb3+/Eu3+complex. Furthermore, a fluorescence sensor array composed of ten sensors was constructed by adjusting the pH and the component of the DPA-Tb3+/Eu3+complex to show multivariate responses towards honey. The visual fluorescence image of the sensor array was recorded by using a smartphone under excitation with portable UV lamp. Results indicated that the pattern of the visual image was related with the botanical origin. After extracting the RGB value of each sensor in 96-well plate, the ratio of R/G was used for principal component analysis (PCA). The results showed that three classes of honey (astragalus, logan, and litchi) were well distinguished. Moreover, the value of principal component 1 (PC1) showed good linearity with the composition of mixing honey and could be used for semi-quantitative analysis. The proposed lanthanide fluorescence sensor array presents a visual and portable method for the discrimination of a honey’s origin without the use of analytical instruments, and might provide a novel and simple strategy for the measurement of food origin.Keywords:honey;botanical origin;lanthanide complex;fluorescence;sensor array
研究领域:
食品科学
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分析化学
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光谱学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ORGANOMETALLIC CHEMISTRY
·
2024
·
IF 2.1
Xueyi Chang,Yongfu Qiu,Zhiyu Cheng,Guiping Tan,Qun Luo,Muqing Chen
This study reports the synthesis of a novel block copolymer, PEO-PFc-PCA, comprising cinnamic acid and ferrocene units via Atom Transfer Radical Polymerization (ATRP), and its application as a positive electrode material in pseudocapacitors. Ferrocene functions as redox-active sites for energy storage, while cinnamic acid, when crosslinked under UV light, forms a nano-network that reduces ferrocene leaching, thereby enhancing the stability and lifespan of the pseudocapacitors. Comprehensive structural characterization, including nuclear magnetic resonance and UV–visible spectroscopy, was conducted on PEO-PFc-PCA. Electrochemical tests—cyclic voltammetry, galvanostatic charge/discharge, and electrochemical impedance spectroscopy—revealed that UV crosslinking improved the electrochemical performance, indicated by a slight increase in current and extended charging plateaus. Specific capacitance and energy density increased by 10 % post-crosslinking, demonstrating improved energy storage capacity. Nyquist plots showed reduced internal and charge transfer resistance after crosslinking, enhancing the electrochemical pathway. Stability tests indicated that crosslinked PEO-PFc-PCA retained 90.1 % of its capacitance after 3000 cycles, compared to 86.6 % pre-crosslinking, highlighting its superior longevity. This research presents a promising approach to enhancing pseudocapacitor energy storage through cinnamic acid photopolymerization, potentially benefiting broader pseudocapacitive electrode designs.
研究领域:
材料科学
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电化学
·
能量存储
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Bingsong Ma,Jiacai Wang,Binxing Zhou,Zihao Wang,Youyi Huang,Cunqiang Ma,Xinghui Li
To explore the impact of harvest season in white tea, high-performance liquid chromatography (HPLC) and amino acid analyzer were developed for the determinations of 17 phenolic compounds, 3 purine alkaloids, 19 amino acids, γ-aminobutyric acid (GABA) and 4 main quality components in 18 Bai mudan sub-type of Xinyang white teas harvested in spring, summer and autumn seasons, respectively. Additionally, the in vitro antioxidant capacity was evaluated by 5 various assays. Principal component analysis (PCA), hierarchical cluster analysis (HCA) and partial least squares-discriminant analysis (PLS-DA) could completely classify these white teas by the harvest season, and indicated its profound impact on chemical composition in white tea. 14 Characteristic compounds (VIP ≥1.0), such as (−)-epigallocatechin (EGC), gallic acid, caffeine, l -theanine, conduced to identify the harvest season of white tea. Through comparison, the spring tea was observed to possess the highest amino acids, catechins contents and in vitro antioxidant capacity. (−)-Epicatechin (EC), EGC, (−)-epicatechin gallate (ECG) and (−)-epigallocatechin gallate (EGCG) were considered as the major antioxidants in white tea due to their highly significantly ( p < 0.01) positive correlations. Overall, the research results provided support for the identification of harvest season in white tea.
研究领域:
食品科学
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化学
·
农业科学
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DOI
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Yanwen Zhuang,Yu Ouyang,Li Ding,Miaowen Xu,Fanfeng Shi,Dan Shan,Dawei Cao,Xiaowei Cao
Early diagnosis of drug-induced kidney injury (DIKI) is essential for clinical treatment and intervention. However, developing a reliable method to trace kidney injury origins through retrospective studies remains a challenge. In this study, we designed ordered fried-bun-shaped Au nanocone arrays (FBS NCAs) to create microarray chips as a surface-enhanced Raman scattering (SERS) analysis platform. Subsequently, the principal component analysis (PCA)-two-layer nearest neighbor (TLNN) model was constructed to identify and analyze the SERS spectra of exosomes from renal injury induced by cisplatin and gentamycin. The established PCA-TLNN model successfully differentiated the SERS spectra of exosomes from renal injury at different stages and causes, capturing the most significant spectral features for distinguishing these variations. For the SERS spectra of exosomes from renal injury at different induction times, the accuracy of PCA-TLNN reached 97.8% (cisplatin) and 93.3% (gentamicin). For the SERS spectra of exosomes from renal injury caused by different agents, the accuracy of PCA-TLNN reached 100% (7 days) and 96.7% (14 days). This study demonstrates that the combination of label-free exosome SERS and machine learning could serve as an innovative strategy for medical diagnosis and therapeutic intervention.
研究领域:
医学诊断
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治疗干预
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纳米技术
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光谱学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Binbin Zhang,Qi Zhou,Xinwei Zhang,Jiao Ma,Xin Cong,Qin Wang,Yonggui Liao,Yajiang Yang,Hong Wang
In this work, 5,5′,5′′-(1,3,5-triazine-2,4,6-triyl)tris(azanediyl)triisophthalate (H 6 L) was used as a gelator to prepare thermostable supramolecular gels (H 6 L gels). The lowest critical gelation concentration of H 6 L was found to be only 0.14 wt%. H 6 L gels remained stable after being placed at 85 °C for 1 h, indicating that its gel-sol phase transition temperature ( T gel ) was not<85 °C. H 6 L gels can be formed in just a few seconds in comparison with the longer gelation time of a conventional thermostable gel. Moreover, a thermostable H 6 L/Fe 3+ gel was also prepared by the coordination interactions of H 6 L and Fe 3+ ions. The studies of FT-IR, UV–vis, XRD and SEM reveal that multiple hydrogen bonds, π-π interactions as well as coordination interactions were the main driving forces for the formation of thermostable three-dimensional network structure of H 6 L gels. In addition, the fluorescence spectra of H 6 L gels showed that the dual emission at 396 nm and 469 nm was derived from the structure of melamine and 5-aminoisophthalic acid contained in H 6 L structure. The gel formed at high temperature had relatively high transparency and strong fluorescence signal, and the fluorescence intensity is affected by the external H + concentration. Using this characteristic, 14 organic acids can be effectively identified with the help of principal component analysis (PCA). L-cysteine can be identified with the naked eye by the color and state changes of H 6 L/Fe 3+ gels.
研究领域:
材料科学
·
化学
·
分析化学
·
超分子化学
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产品关联
DOI
高影响因子
Foods
·
2020
·Vol. 9
·期 4
·505
·
IF 4.092
Qi Zhou,Guijie Li,Zhu Ou-Yang,Xin Yi,Linhua Huang,Hua Wang
An efficient and practical method for identifying mandarin juice over-blended into not from concentrate (NFC) orange juice was established. Juices were extracted from different cultivars of sweet orange and mandarin fruits. After being pasteurized, the volatile organic compounds (VOCs) in the juice samples were extracted using headspace solid-phase microextraction, and qualitatively and quantitatively analyzed using gas chromatography–mass spectrometry detection. Thirty-two VOCs contained in both the sweet orange juice and mandarin juice were used as variables, and the identification model for discriminating between the two varieties of juice was established by principal component analysis. Validation was applied by using common mandarin juices from Ponkan, Satsuma and Nanfengmiju cultivars blended at series of proportions into orange juices from Long-leaf, Olinda, and Hamlin cultivars. The model can visually identify a blending of mandarin juice at the volume fraction of 10% or above.Keywords:mandarin juice;not from concentrate (NFC);principal component analysis (PCA);headspace solid-phase microextraction (HS-SPME)
研究领域:
食品科学
·
分析化学
·
农业化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2024
·
IF 4.6
Ruiyuan Zhang,Rui Hao,Jixiang Fang
Tumor exosomes, known as maternal cell messengers, play an important role in cancer occurrence, proliferation, metastasis, immune escape, drug resistance, and other processes and are an entry point for cancer research. However, there is still a lack of an efficient detection technology for exosomes. In this study, the ultrahigh sensitivity SERS nanoprobe with a three dimensional (3D) magnetic core/Au nanocolumn/Au nanoparticles shell strongly coupling multistage structure (Fe3O4@NR-NPs) was constructed by crystal growth of nanocrystals in the confined space of a central radiating single particle mesoporous molecular sieve channel and strong coupling secondary growth of gold particles. The exosomes were confined onto the “hot spot” of plasmonic nanoparticles and rapidly enriched by CD63 antibody functional-Fe3O4@NR-NPs to achieve high sensitivity detection, with the limit of detection of 1 × 103 particles/mL (S/N = 3). The spectral data set of different exosomes is applied to train for multivariate classification of cell types and to estimate how the normal exosome data resemble cancer cell exosomes by principal component analysis (PCA). Finally, this detection method has also been successfully employed for the detection of exosomes in complex samples; this proves that the proposed SERS-based method is a promising tool for clinical cancer screening.
研究领域:
纳米技术
·
癌症生物学
·
分析化学
·
生物标志物研究
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Morui Wang,Haoxuan Diao,Cheng Dou,Zhenye Wu,Liguang Ruan,Zhixin Wang,Zhong Wang,Weichun Ye,Jiangong Duan,Yuee Li
This work aims to face the challenge of label-free and quantitative detection for pathogenic microorganisms concerning on food safety and medical diagnosis. Ag NPs@PDMS sponge SERS substrates were developed through the "mold transfer-surface embedding" method for pathogenic microbial detection. The pore structure provided large specific surface area and critical contact sites, which endowed the detection limit of only 1 CFU/mL for E.coli . Importantly, six different microorganisms could be identified using this SERS substrate. The classification models combining three types of data dimension reductions and five types of machine learning algorithms were constructed to achieve the automatic classification of microorganisms. Among these algorithms, the PCA-SVC model held the highest accuracy of 92.17%. Different from labeled SERS, label-free SERS technique is conducive to the discovery of unknown microorganisms in water-environment. Therefore, this SERS substrate is expected for microbial system assessment and microbial safety monitoring, combined with handheld Raman spectrometer and artificial intelligence.
研究领域:
分析化学
·
微生物学
·
生物医学工程
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机器学习
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食品安全
·
医学诊断
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Chengna Xu,Ji Zhou,Yong Ye,Bin Tang
Peroxidase-like reaction process involving o-phenylenediamine (OPD) and silver nanoprisms in the presence of hydrogen peroxide (H 2 O 2 ) was monitored using time-resolved ultraviolet–visible (UV–Vis) absorption spectroscopy. The oxidation of OPD and etching of silver nanoprisms were investigated by analyzing the dynamic spectral data. Two-dimensional correlation spectroscopy (2D-COS) and principal component analysis (PCA) were employed to gain insights into the correlation between catalytic oxidation of OPD and etching of silver nanoprisms. It was found that OPD offered significant protection effect for silver nanoprisms so that morphologies of silver nanoprisms maintained at the beginning period after addition of H 2 O 2 . Moreover, silver nanoprisms accelerated the oxidation of OPD by H 2 O 2 , demonstrating enzymatic mimicking activity of silver nanoprisms. The combination of time-resolved UV–Vis absorption spectroscopy and spectral calculation methods could be used for exploration of complex reaction systems with spectral variations.
研究领域:
纳米技术
·
催化
·
光谱学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）