Biomaterials Science
·
2020
·Vol. 8
·期 8
·2129-2142
·
IF 6.183
Xiaoshuang Guo,Zihan Yuan,Yang Xu,Minyan Wei,Zhiwei Fang,Wei-En Yuan
Hemangioma, one of the most common angiogenic diseases in infants and children, is characterized by the abnormal and aggressive proliferation of vascular endothelial cells. Advanced therapeutic strategies like RNA interference can inhibit the expression of target proteins at the translational level, but they are rarely used in hemangioma treatment owing to the lack of safe carriers. In this study, we showed for the first time that RNAi technology targeting HIF-1α (hypoxia-inducible factor-1 alpha) could benefit hemangioma therapy effectively. Heptafluorobutyric anhydride (HFAA) was used to modify low-molecular-weight PEI (PEI1.8k), and a novel fluorinated polycation carrier named fluorinated PEI (FPEI) was synthesized. Furthermore, HIF-1α-shRNA-pDNA was condensed by FPEI to fabricate FPEI polyplexes. Compared with PEI25k polyplexes, which are usually the gold standard used in gene delivery, FPEI polyplexes showed lower cytotoxicity and higher serum stability, transfection efficiency and gene silencing efficiency both in vitro and in vivo. In addition, we confirmed that FPEI polyplexes could efficiently inhibit the formation of new capillaries and tumor growth in vivo, which may provide a practicable strategy for clinical hemangioma treatment in the future.
研究领域:
药理学
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分子生物学
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生物医学工程
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治疗学
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DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Linlin Hao,Gewei Feng,Zengli Zhai,Mingxuan Li
It has been accepted that limiting phosphate is a prerequisite for bacterial inactivation in tap water. La 2 O 3 nanoparticles were loaded onto polypropylene (PP) fibers through the method of melt spinning. Hydrophilic modification of the adsorbent surface was performed using polyethyleneimine (PEI) to prepare PEI/La 2 O 3 /PP. The results indicated that the optimal loading amount of La 2 O 3 was 1 %, and the optimal concentration of PEI was 1 %. The successful loading of La 2 O 3 resulted in a roughened fiber surface and increased fiber diameters . After PEI modification the water contact angle was reduced from 98° to 50°. The pH zpc of PEI/La 2 O 3 /PP was determined to be 8.3. The co-existing ions have little effect on phosphate adsorption. Phosphate adsorption can be well described by Redlich-Peterson model with a maximum K R-P of 3.98 × 10 −5 L/mg. Adsorption kinetics fit well with the pseudo-second-order reaction equation. The activation energy (E a) is determined to be 57.9 kJ/mol, providing the evidence of chemical nature of the reaction involving phosphate ions on the surface of PEI/La 2 O 3 /PP. The PEI/La 2 O 3 /PP adsorbent can reduce the phosphate concentration in tap water to below 0.01 mg/L, leading to a reduction in Escherichia coli concentration from approximately 10,000 CFU/mL to around 10 CFU/mL. The survival rates of Escherichia coli in a multi-nutrient environment are higher than in a single phosphate source environment. Our research provides a new feasible approach for ensuring microbial water quality safety in household water purifiers.
研究领域:
环境科学
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材料科学
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微生物学
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产品关联
DOI
高影响因子
Gas Science and Engineering
·
2025
·
IF 5.5
Chenyang Lin,Yijun Wang,Yihang Liu,Takeshi Hagio,Baowen Zhou,Xinling Li,Zhen Huang
Direct air capture (DAC) is a technology that captures low concentrations of CO 2 directly from the air. In recent years, amine-modified solid adsorbents have been widely utilized to facilitate an efficient CO 2 capture process. In industrial applications, the significant pressure drop reduced by powder accumulation results in economic losses for fans. However, previous studies on monoliths have failed to simultaneously satisfy the industrial requirements for both adsorption capacity and mechanical strength. In this work, a novel honeycomb monolith was proposed to address this issue. The seven uniformly distributed channels effectively reduced pressure drop, while the wall thickness satisfied the mechanical strength requirements under industrial application conditions. And the structure was optimized by using activated carbon. After high-temperature treatment, the new pore formed in the space previously occupied by the activated carbon. Through the secondary loading approach, the amine loading was comparable to the powder. Compared with the monoliths without activated carbon, the pore volume significantly increased (from 5.31 % to 96.26 %). The pore volume of 20AC-SiO 2 -M reached 0.84 cm 3 g −1 . At 35 °C and 400 ppm, 20AC-SiO 2 -M exhibited the best CO 2 adsorption capacity (1.94 mmol/g) and the fastest CO 2 adsorption rate (0.0395 mmol/(g.min)). Additionally, the structure strength (1.46 MPa) and pressure drop losses (0.002 psi) were tested, and both parameters met the requirements for industrial applications.
研究领域:
环境科学
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材料科学
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化学工程
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吸附分离
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碳捕集与封存
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DOI
高影响因子
International Journal of Nanomedicine
·
2019
·
IF 4.471
Fei Tong,Xiangyuan Tang,Daojun Liu
Background Hepatic ischemia/reperfusion-induced pancreatic islet injury (HI/RIPII) was an important pathophysiological phenomenon in clinics. In the present study, we observed the effects of phycocyanin on HI/RIPII. However, the half-life of phycocyanin was extremely short and limited its use in vivo.Materials and methods In order to overcome this shortcoming, poly(ethylene glycol)-b-(poly(l-glutamic acid)-g-polyethylenimine) (PEG-b-(PG-g-PEI)) was synthesized and estimated as a nanocarrier for lengthening delivery of phycocyanin through the abdominal subcutaneous injection in rats. Phycocyanin (isoelectric point=4.3) was encapsulated with PEG-b-(PG-g-PEI) via electrostatic interactions at pH 7.4.Results In vitro phycocyanin was fast and efficiently encapsulated and showing efficient loading and sustained release. In vivo the anti-HI/RIPII function of phycocyanin/PEG-b-(PG-g-PEI) complex was surveyed in rats using free phycocyanin as the controls, and the results showed that phycocyanin/PEG-b-(PG-g-PEI) complex reduced HI/RIPII property and enlarged islet functionality.Conclusion These results suggested that PEG-b-(PG-g-PEI) might be treated as a potential phycocyanin nanocarrier.
研究领域:
药理学
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纳米技术
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医学
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产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2023
·
IF 9.9
Shao-Lu Li,Yaxu Guan,Yiwen Qin,Youcai Chen,Dandan Cheng,Genghao Gong,Yunxia Hu
In this study, a novel antifouling loose nanofiltration (LNF) membrane was prepared by the cross-linking and grafting of hydrolyzed polyacrylonitrile ultrafiltration (HPAN UF) substrate with a polyethylenimine (PEI) based zwitterionic polymer PEI-CA through the covalent amide bond. The successful introduction of PEI-CA was verified by various techniques. The tailored membrane HPAN-PEICA exhibited ultra-high water permeance (73.5 L m −2 h −1 bar −1 ) and meanwhile maintained excellent rejection (~99.9 %) for dye Brilliant Blue R (BBR), Methyl Blue (MB), Congo red (CR) and Chrome Black T (CBT). In addition, it exhibited ultralow rejection (below ~6 %) towards the inorganic salts (NaCl, Na 2 SO 4 , MgCl 2 and MgSO 4 ), with excellent dye/salt selectivity of ~65. The as-fabricated membrane HPAN-PEICA was proved having good long-term operation stability and pressure resistance properties. Dynamic fouling experiments confirmed that the membrane HPAN-PEICA presented excellent antifouling performance towards four typical model foulants (LYZ, SA , HA and BSA). Over all, we believed that the tailored LNF membrane HPAN-PEICA having great application prospects in textile effluent treatment.
研究领域:
材料科学
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环境工程
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 6
·1-10
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IF 5.3
Ren Dongxia,Wang Lin,Wei Hua,Mu Shijie,Yang Longfei
Bacterial infection is one of the leading causes of human death globally and is considered to be an urgent public health challenge. It is important to accurately and rapidly detect and kill pathogenic microorganisms. Therefore, a multifunctional platform for sensitive detection and efficient elimination of pathogenic bacteria should be developed to reduce the threat of bacteria. In this study, an integrated strategy based on Fe 3 O 4 -quaternary ammonium chitosan-polyetherimide-Cu-aptamer (Fe 3 O 4 -QCS-PEI-Cu-aptamer) and ladder-branch hybridization chain reaction (HCR) was proposed for the detection and inactivation of Staphylococcus aureus ( S. aureus ). The aptamer-complementary chain (aptamer-CP) conjugated to the surface of Fe 3 O 4 -QCS-PEI-Cu induces CP release in the presence of S. aureus , further initiating the ladder-branch HCR reaction and achieving signal amplification. Under optimal conditions, the developed sensor demonstrated a limit of detection (LOD) of 3 CFU/mL for S. aureus and exhibited high selectivity against several other pathogenic bacteria. Moreover, the Fe 3 O 4 -QCS-PEI-Cu-aptasensor showed potent antibacterial properties under near infrared (NIR) irradiation, achieving an antimicrobial efficiency of 99.946%. This strategy synergistically combined the high-sensitivity molecular detection with the antibacterial activity of novel nanomaterials activated by near infrared light, providing a new insight into the prevention of bacterial infections. Graphical
研究领域:
生物医学工程
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微生物学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ruonan Wang,Baoshan He,Zhengyong Liang,Yao Liu,Jun Yang,Huali Jin,Min Wei,Wenjie Ren,Zhiguang Suo,Yiwei Xu
This study presents an integrated dual-mode sensing strategy, in which qualitative fluorescence screening is followed by quantitative electrochemical detection, improving detection efficiency and enabling rapid PCM analysis. It develops a novel fluorescence electrochemical aptasensor that combines in vitro “Dark-box” applications with CRISPR/Cas12a system electrode surface sensing technology. PCM activates the DNA walker, and the DNAzyme induces cyclic cleavage of DNA strands bearing the Carboxyfluorescein (FMA) group. After magnetic separation, the fluorescence reaction combined with the “Dark box” enables the preliminary qualitative analysis of procymidone (PCM). Following the preliminary qualitative detection, the solution is introduced to the electrochemical aptasensor platform integrated with the CRISPR/Cas12 system. The Cas12a system triggers cyclic cleavage, producing a signal change that enables the electrochemical quantitative detection of PCM. An fluorescence (FL) response occurs when the PCM concentration in spiked samples is at or above 1 pg·mL −1 , allowing for qualitative fluorescence detection. The EC platform's detection limit is 8.51 × 10 −6 ng·mL −1 , with a range of 1 × 10 −2 ng·mL −1 to 1 × 10 4 ng·mL −1 . The designed dual-mode sensor provides reliable monitoring of PCM in real samples.
研究领域:
生物传感技术
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食品安全检测
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分子生物学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2013
·Vol. 4
·期 2
·794-804
·
IF 2.562
Han Zhu,Ming Zhang,ShengYing Cai,YingTing Cai,Pan Wang,ShiYong Bao,MeiLing Zou,MingLiang Du
A facile approach for the synthesis of uniform, small size and well-dispersed rhodium nanoparticles (RhNPs) on cross-linked polyvinyl alcohol–polyethyleneimine (PVA–PEI) nanofibers has been demonstrated. Various methods were firstly employed to cross-link PVA nanofibers and the cross-linked PVA–PEI nanofibers exhibited good water stability and porous structures after immersing in water for 72 h. Because of the strong chelate effects among the amine groups, hydroxyl groups and Rh3+ ions, uniform RhNPs with an average diameter of about 2.5 ± 0.2 nm can evenly and densely grow throughout the PVA–PEI nanofibers via an in situ reduction. Meanwhile, the better dispersion and smaller size of the RhNPs grown on the nanofibers in comparison with the pre-synthesized RhNPs directly deposited on the nanofibers exhibit the advantages of in situ reduction for size and dispersion control. The successful fabrication of the RhNPs/(PVA–PEI) nanofibers with various densities of well-dispersed RhNPs demonstrates that the strong chelate effects and stabilization of the PVA–PEI nanofibers also play an essential role in the size and dispersion control of RhNPs. The crystal structures, chemical bonding and interactions of the prepared nanofibers were verified using XPS and FTIR spectra and XRD patterns. These novel nanomaterials were fabricated as non-enzymatic electrochemical sensors and exhibit highly electrocatalytic activity towards H2O2.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Min Wu,Jian Yang,Shunli Chen,Peng Lu,Ruifang Wang
A nanocellulose-based film with excellent antimicrobial and antioxidant activity was developed by adding Pickering emulsion which was stabilized by functionalized particles. First, TOCNC- g -PEI nanoparticles were prepared by grafting polyethyleneimine (PEI) onto TEMPO-oxidized cellulose nanocrystals (TOCNCs) and used to stabilize oregano essential oil (OEO) Pickering emulsions. The contact angle of TOCNC- g -PEI nanoparticles was 79.7°, and the minimum inhibitory concentration against L.monocytogenes and E.coli was 0.50 mg/mL. Second, the emulsion droplets were stably dispersed in cellulose nanofibril (CNF) suspensions owing to the depletion stability of CNFs. Finally, the films were dried and emulsion droplets formed oil core/CNF shell microcapsules in active CNF films and completely encapsulated OEO in active CNF films. The inhibition rates of the film against L.monocytogenes and E.coli were 97.28% and 97.23%, respectively. The influence of Pickering emulsion on the active CNF films was discussed. The developed active CNF films have promising application in food preservation and active packaging.
研究领域:
食品科学
·
材料科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2023
·
IF 9.9
Tixi He,Fengjun Chen
Thin-film composite (TFC) membranes with an interlayer were prepared extensively for desalination , but the hydrophilicity and permeability were not well improved. In this work, a smooth and hydrophilic interlayer was constructed by electrostatic air spray deposition (EASD) of poly (sodium 4-styrenesulfonate) (PSS) and polyethyleneimine (PEI) on the polyethersulfone substrate and an ultra-thin separation layer was prepared using interfacial polymerization of piperazine (PIP) and trimesoyl chloride (TMC) to acquire high-performance composite nanofiltration (NF) membranes. The polymer solution was atomized into tiny particles under a high-pressure electrostatic air spray effect, facilitating the deposition of PSS/PEI with good homogeneity and enabling a denser and smoother interlayer surface. The introduction of PSS/PEI enhanced the hydrophilicity of TFC membranes and increased the repulsion of divalent cations to improve the salt rejection. The impacts of PEI concentration and EASD time on the separation performance of NF membrane were explored. The optimized TFC-3 NF membrane had a permeate flux of 74.5 ± 1.6 L·m −2 ·h −1 under 0.5 MPa, which was 2.5 times higher than the water flux of conventional TFC-0 membrane, while retaining up to 99.2 ± 0.5 % of Na 2 SO 4 . This work provides a prospective strategy to develop high-performance TFC membranes with great extensive progress for seawater purification.
研究领域:
膜科学
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水处理
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiubing Zhang,Hongye Wu,Zhili Li
Traditional homogeneous Fenton systems face limitations, including a narrow pH range, potential secondary pollution, and poor repeatability. In this study, these bottlenecks in tetracycline wastewater treatment were addressed with using carbonized porous polyethyleneimine-grafted lignin microspheres (PLMs) supported Fe-loading catalysts (PLMs/Fe-C). An optimized PLMs/Fe-C catalyst under specific conditions (carbonization temperature: 350 °C, PLMs: Fe = 1:1, and alkali lignin: PEI = 1:4) was developed, which proved to be an efficient Fenton-like catalyst for tetracycline (TC) degradation. TC degradation results showed that the PLMs/Fe-C + H 2 O 2 system achieved a high removal efficiency of 100 % within 150 min (50 mL TC with an initial concentration of 50 mg/L, 0.015 g catalyst, 0.5 mL H 2 O 2 ), demonstrating its applicability over a wide pH range (3−10) and the capability to remove multiple pollutants (2,4-D, BPA, Lev, etc.). This efficiency was attributed to the stronger electron-donating ability and mass transfer properties derived from the porous structure and functional groups. Arrhenius fitting analysis revealed a lower activation energy (E = 52.98 kJ/mol) for the PLMs/Fe-C, indicating a more accessible reaction. Additionally, the removal efficiency marginally decreased from 100 % to 98.32 % after five cycles, demonstrating excellent reusability. Mechanistic investigations indicated that the PLMs/Fe-C can activate H 2 O 2 to produce reactive oxygen species •OH, 1 O 2 , effectively degrading TC.
研究领域:
环境科学
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催化材料
·
废水处理
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 37
·4320-4327
·
IF 2.896
Ying Zhang,Yuhan Ning,Wanliang Liao,Weiping Wang,Ai-Jun Wang
In this work, COF-LZU1@PEI@Fe3O4 was synthesized by immobilization of COF-LZU1 onto the surface of polyethyleneimine-functionalized Fe3O4 nanoparticles (PEI@Fe3O4) and employed as an adsorbent for magnetic dispersive micro-solid phase extraction of tetracyclines (TCs). COF-LZU1@PEI@Fe3O4 was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and nitrogen adsorption–desorption isotherms analysis. The optimal extraction and desorption conditions were as follows: 15.00 mL sample solution (pH 7.0) extracted with 5.0 mg of adsorbent for 30 min at 30 °C, and then desorbed with 1.50 mL methanol/acetonitrile/0.02 mol L−1 oxalic acid solution (v/v, 1 : 2 : 7). Good linearities were obtained between the peak area and TC concentration ranging from 5–500 μg L−1 with correlation coefficients (R2) higher than 0.9992 and limits of detection lower than 0.51 μg L−1. The relative standard deviations (RSDs) of intra-day and inter-day were less than 5.7% and 7.4%, respectively. The developed method was successfully applied to the determination of TCs in environmental water samples with recoveries in the range of 87.0–113.8% and RSDs less than 5.1%, suggesting great potential of COF-LZU1@PEI@Fe3O4 for efficient extraction and analysis of trace TCs in water samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）