ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2020
·
IF 4.872
Yi Qian,Yunhao Zhang,Achuo Anitta Zuh,Weichuan Qiao
Per- and polyfluoroalkyl substances (PFASs) are toxic to microorganisms , thereby affecting microbial communities in sludge and soil, but how to repair the toxicity of microorganisms remains unclear. In this study, rutin, an antioxidant, was added into a culture medium with an aerobic denitrification bacteria, Pseudomonas stutzeri , under the exposure of sodium perfluorononyloxy-benzenesulfonate (OBS) to evaluate the repair mechanisms of rutin to the toxicity of OBS to the bacteria. The results showed that rutin could repair the damage of OBS to cell structures, and reduce the death rates of the bacteria under OBS exposure. The dosage of rutin reduced the effect on the inhibition of denitrification ability of P. stutzeri under OBS exposure. Compared with the bacteria exposed to single OBS, the dosage of rutin resulted in that the death rates recovered from 96.2% to 66.4%, the growth inhibition rate decreased from 46.5% to 15.8%, the total nitrogen removal rate recovered from 66.9% to 100%, and the NO 2 − content recovered from 34.5 mg/L to 0.22 mg/L. The expressions of key denitrification genes ( napA , nirS , norB , nosZ ) were recovered after adding rutin under OBS exposure. Rutin changed the positive rate of reactive oxygen species , the relative superoxide dismutase and catalase activities in the bacteria which exposed to OBS. The mechanism by which rutin repaired the toxicity of OBS to P. stutzeri related to inhibiting the activities of antioxidant and denitrification enzymes rather than affecting the expressions of genes involved in these enzymes. This study sheds light on the repair method of micro-organics and reveals the repair mechanisms under PFASs exposure.
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WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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2018
·Vol. 34
·期 9
·1-10
·
IF 2.1
Peng Huasong,Tan Jian,Bilal Muhammad,Wang Wei,Hu Hongbo,Zhang Xuehong
Phenazine-1-carboxamide (PCN) is one of the major biocontrol agents produced by plant growth-promoting rhizosphere (PGPR) pseudomonads including Pseudomonas chlororaphis . In this study, a combined strategy of genetic modification and statistical experimental designs was applied to obtain mutants of P. chlororaphis strains with high-yield PCN production. To achieve this, the lon gene was knocked out in wild-type P. chlororaphis HT66 and the breeding mutant P3 strain with a non-scar deletion strategy. The resulting HT66Δ lon and P3Δ lon mutants produced a significantly higher PCN production in shake-flask cultures which was 5- and 9-folds greater than their native counterparts. The potential ability of strain P3 Δlon for PCN production was further optimized by statistical designs. A two-level Plackett–Burman (PB) experimental design with six variables was employed to scrutinize medium components that significantly influence PCN production. Notably, glycerol, tryptone, and soy peptone were identified to be the most significant factors ( p < 0.05). Response surface methodology (RSM) based on the central composite design (CCD) was adopted to determine these factors optimal levels and their interactive effects between culture components for PCN production. The predicted maximum PCN production was 9002 mg/L, whereas an actual PCN production of 9174 mg/L was recorded in the validation experiments using the optimal medium containing glycerol 37.08 mL/L, tryptone 20.00 g/L, and soy peptone 25.03 g/L, which was nearly threefolds higher than without optimization and 20-folds higher than the wild-type strain. In conclusion, the results revealed that P. chlororaphis display a high potential for industrial-scale production for phenazine biopesticides.
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INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2014
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IF 2.235
Meng Lin,Xiaoke Hu,Weiwei Chen,Hui Wang,Chuanyuan Wang
A phenanthrene (PHE) degrading bacterium strain BZ-3 was isolated from the crude oil contaminated soil in Binzhou, China. The isolate was identified as Pseudomonas sp. BZ-3 on the basis of 16S rRNA gene sequence. Various experiments were conducted to investigate the effect of pH, salinity and PHE concentration on the degradation efficiency of PHE. The degradation efficiency and degradation metabolites of PHE were detected by using GC–MS and HPLC-MS analyses. The strain BZ-3 could degrade 75% of PHE at an initial concentration of 50 mg/L under 20 g/L salinity in 7 days. PHE degradation kinetics was estimated in a first-order degradation rate model and the rate coefficient was calculated as 0.108 d −1 . On the basis of the identified degradation metabolites, the strain BZ-3 could degrade PHE in the salicylate metabolic pathway. In a mixture system consisting of PHE and other PAHs including naphthalene (NA), anthracene (ANTH), and pyrene (PYR), the strain BZ-3 showed an efficiently degradation capability. Further study showed that the strain BZ-3 could also use NA, ANTH, PYR, xylene, 1-hydroxy-2-naphthoic acid, and hexane as the sole carbon and energy source, but did not grow on nitrobenzene-containing medium.
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mBio
·
2024
·Vol. 15
·期 6
·
IF 5.1
Xiaolong Guo,Hua Yu,Junzhi Xiong,Qian Dai,Yuanyuan Li,Wei Zhang,Xiping Liao,Xiaomei He,Hongli Zhou,Kebin Zhang
Pseudomonas aeruginosa is one of the most common nosocomial pathogens worldwide, known for its virulence, drug resistance, and elaborate sensor-response network. The primary challenge encountered by pathogens during the initial stages of infection is the immune clearance arising from the host. The resident macrophages of barrier organs serve as the frontline defense against these pathogens. Central to our understanding is the mechanism by which bacteria modify their behavior to circumvent macrophage-mediated clearance, ensuring their persistence and colonization. To successfully evade macrophage-mediated phagocytosis, bacteria must possess an adaptive response mechanism. Two-component systems provide bacteria the agility to navigate diverse environmental challenges, translating external stimuli into cellular adaptive responses. Here, we report that the well-documented histidine kinase, LadS, coupled to a cognate two-component response regulator, PA0034, governs the expression of a vital adhesin called chaperone-usher pathway pilus cupA. The LadS/PA0034 system is susceptible to interference from the reactive oxygen species likely to be produced by macrophages and further lead to a poor adhesive phenotype with scantily cupA pilus, impairing the phagocytosis efficiency of macrophages during acute infection. This dynamic underscores the intriguing interplay: as macrophages deploy reactive oxygen species to combat bacterial invasion, the bacteria recalibrate their exterior to elude these defenses.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Mingliang Zhang,Weihao Zhu,Hongfei Liu,Kaihua Pan,Qian Li,Qian Zhu,Yanni Huang,Changchang Wang,Junqiang Hu,Mingli Jiang,Xin Yan,Qing Hong
Acetaminophen (APAP), an analgesic and antipyretic drug, is commonly detected in wastewater treatment plant (WWTP) effluents, surface water, and soil, indicating its status as an emerging environmental contaminant. In this study, we isolated a bacterium, Pseudomonas taiwanensis AP-1, capable of completely mineralizing APAP and utilizing it as the sole carbon source for growth. A newly identified metabolite, γ-glutamyl-4-aminophenol (γ-G4AP), was reported for the first time in the degradation of APAP by strain AP-1. Two amidases (ApaH1 and ApaH2), responsible for the conversion of APAP to 4-aminophenol (4-AP), were identified through a combination of genomic comparison, heterologous expression, and gene knockout. Notably, ApaH1 played a pivotal role in the degradation of APAP by strain AP-1. The catalytic triad of ApaH1 (K82-S161-S185) and ApaH2 (K85-S160-S184) were identified as by molecular docking and site-directed mutagenesis. Additionally, a gene cluster apd for the metabolism of 4-AP was also successfully identified in strain AP-1, consisting of the aniline dioxygenase gene cluster apdBCD1D2EF and the BT catabolic gene apdGH . Interestingly, the 4-AP metabolic gene cluster apd was highly conserved among other Pseudomonas strains capable of APAP degradation. Our results provide new insights into the mechanism of APAP biodegradation and strain AP-1 may be a promising bacterium for the bioremediation of APAP pollutions.
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IEEE TRANSACTIONS ON NANOBIOSCIENCE
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2026
·
IF 4.2
Wanlu Zheng,Weixin Kong,Na Zhang,Zhong Li,Ya-nan Zhang
reusable optical fiber surface plasmon resonance (SPR) sensor for quantitative detection of Pseudomonas aeruginosa is presented. To achieve repeatable measurement, this study used aptamer DNA as the functional monomer and polydopamine as the cross-linking agent to construct a molecularly imprinted nanofilm on the sensing surface. After elution treatment, molecularly imprinted cavities with high matching shape and size to the target molecule are formed in the nanofilm. Experimental results demonstrated that the sensors enhanced by molecular imprinting technology achieved an increase in resonance wavelength response from 10.677 nm to 24.98 nm, a reduction in response time from 22 minutes to 16 minutes, and an improvement in detection limit from 0.00985 OD to 0.00105 OD, compared with sensors only modified with functional monomers. Moreover, to address the problem of temperature interference in practical applications, a Fabry-Perot interferometer (FPI) is innovatively integrated. By taking advantage of the temperature sensitivity but bacterial insensitivity of FPI, the influence of temperature changes on SPR measurement is quantified, effectively providing temperature compensation and further improving the environmental adaptability of the sensor. This study provides a novel, reliable, and cost-effective detection solution for marine environmental microbial monitoring.
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npj Materials Degradation
·
2025
·Vol. 9
·期 1
·1-10
·
IF 7.6
Shi Xin,Zhang Yimeng,Zhang Ruiyong,Li Chengpeng,Wang Can,Wang Xiao,Sand Wolfgang,Duan Jizhou
Biocides are widely employed to mitigate microbiologically influenced corrosion (MIC) in oil and gas fields. However, improper biocide dosing may result in changes to the MIC. This study investigated the corrosion behavior of the biofilm-forming Pseudomonas stutzeri on X70 pipeline steel under sublethal concentrations of tetrakis(hydroxymethyl) phosphonium sulfate (THPS) using cell counting, weight loss measurements, surface characterization, and transcriptomic analysis. The results revealed that THPS accelerated the corrosion of X70 steel in the presence of P. stutzeri , compared to THPS-free conditions. While THPS inhibited the growth of planktonic cells, it promoted biofilm formation on the X70 steel surface. Transcriptomic data suggested that this corrosion acceleration could be attributed to metabolic alterations in P. stutzeri , potentially enhancing electron transfer between bacterial cells and the steel surface. These findings provide guidance for the rational application of biocides in industrial settings.
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Nature Communications
·
2026
·
IF 18.1
Ye Yingmin,Zhang Kai,Wang Yanmin,Li Yang,Zhao Nana,Xu Fu-Jian
The rapid emergence of antimicrobial resistance is a critical global health challenge that renders conventional antibiotics ineffective. Developing innovative strategies to resensitize drug-resistant pathogens to existing antibiotics represents a promising therapeutic approach. Here, we present a biomimetic nanoplatform (Ce-Car@EV NPs) through the rational integration of ginger-derived extracellular vesicles (EVs) and a pH-responsive cerium-carbenicillin coordination nanoparticles (Ce-Car NCPs). This design enables prolonged circulation and targeted degradation in acidic infection sites, releasing Ce 4+ ions and carbenicillin. The released Ce 4+ ions penetrate the bacterial cells, where they disrupt ATP synthesis, impede oxidative phosphorylation, and inhibit the activity of efflux pump. By depleting ATP, blocking efflux pumps, and thereby reversing bacterial resistance, Ce 4+ ions act as a potent adjuvant to carbenicillin. Here we show that this strategy effectively restores carbenicillin efficacy against drug-resistant Pseudomonas aeruginosa both in vitro and in vivo, establishing a therapeutic strategy leveraging metallic adjuvants to counteract antimicrobial resistance.
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FUEL
·
2023
·
IF 7.4
Chen Shi,Xiangrong Liu,Shun-Sheng Zhao,Zaiwen Yang
The biodegradation of Inner Mongolia coal by Pseudomonas japonica ( P. japonica ) was investigated, showing that the biodegradability of P. japonica was related to its secretions: esterase (Es) and rhamnolipid (Rh). Therefore, the efficiencies of coal biodegradation were explored through additions of Es, Rh and the mixture of Es and Rh (Es-Rh) to the biodegradation system, respectively. It was found that the coal biodegradation rate all increased in the presence of Es-Rh, Es or Rh, among which Es-Rh significantly increased the coal biodegradation rate. When the ratio of Es-Rh was 150 mg/mL:1200 mg/mL, the coal biodegradation rate reached a maximum of 65.3% higher than the control group 18.8%, it was because the optical density and esterase activity of P. japonica were the highest, while the electronegativity and adsorption rate of coal samples were the optima. GC–MS of the degradation liquid products showed that the compositions of degradation liquid products in the presence of Es-Rh mainly contained long-chain alkanes, alcohols and ethers, of which the highest content of long-chain alkanes was 81.13%. FTIR analysis confirmed that the C H, C C and -COOR groups in Inner Mongolia coal were degraded in the presence of Es-Rh. XRD analysis demonstrated that the L c and N ave decreased in the presence of Es-Rh, indicating that the crystallization degree of Inner Mongolia coal decreased. This study indicated that the addition of mixture of Es and Rh could significantly promote the degradation of Inner Mongolia coal by P. japonica .
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-15
·
IF 4.6
Zheng Xiaoran,Gao Min,Wu Liangquan,Lu Xin,Lin Qiuqi,Zhong Hai,Lu Yingfei,Zhang Yunlei,Zhang Xiuwei
Pseudomonas aeruginosa ( P. aeruginosa ) infections present a grave threat to immunocompromised individuals, particularly those with cystic fibrosis due to the development of bacterial biofilms. In this study, we engineered self-assembling chitosan-ceftazidime nanoparticles (CSCE) capable of effectively penetrating biofilms and eradicating P. aeruginos a. The CSCE nanoparticles were synthesized through ionic cross-linking, combining negatively charged ceftazidime with positively charged chitosan, resulting in uniform nanoparticles measuring approximately 40 nm in diameter, exhibiting high dispersity and excellent biocompatibility. Remarkably, these nanoparticles exhibited significant inhibition of P. aeruginosa growth, reduced pyocyanin production, and diminished biofilm formation, achieving a maximum inhibition rate of 22.44%. Furthermore, in vivo investigations demonstrated enhanced survival in mice with abdominal P. aeruginosa infection following treatment with CSCE nanoparticles, accompanied by reduced levels of inflammatory cytokines Interleukin-6 (125.79 ± 18.63 pg/mL), Interleukin-17 (125.67 ± 5.94 pg/mL), and Tumor Necrosis Factor-α (135.4 ± 11.77 pg/mL). Critically, mice treated with CSCE nanoparticles showed no presence of bacteria in the bloodstream following intraperitoneal P. aeruginosa infection. Collectively, our findings highlight the potential of these synthesized nanoparticles as effective agents against P. aeruginosa infections.
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Frontiers in Cellular and Infection Microbiology
·
2022
·
IF 6.073
Yin Lujun,Shen Wang,Liu Jun-Sheng,Jia Ai-Qun
Siderophore is necessary for the survival of microorganisms and is interregulated with quorum sensing (QS) systems. It is related to growth, proliferation, virulence, and other bacterial social activities as a virulence factor. Thus, we speculated that the QS system could be occluded by inhibiting siderophore production. 2-Hydroxymethyl-1-methyl-5-nitroimidazole (HMMN), one siderophore inhibitor of Pseudomonas aeruginosa PAO1 (P. aeruginosa PAO1), was obtained by using the Chromeazurol S (CAS) method. We found that HMMN inhibited siderophore production and influenced the biological effects of QS regulation, including biofilm formation and pyocyanin production. HMMN (150 μg/ml) inhibited the siderophore production of P. aeruginosa PAO1 by 69.37%. In addition, HMMN could inhibit pyocyanin production and biofilm formation and erase the formed biofilm of P. aeruginosa PAO1. HMMN (150 μg/ml) inhibited the biofilm formation of P. aeruginosa PAO1 by 28.24%. The erasure rate of the formed biofilm reached 17.03%. Furthermore, HMMN (150 μg/ml) inhibited P. aeruginosa PAO1 pyocyanin production by 36.06%. Meanwhile, positive-control hordenine (500.0 μg/ml) reduced the biofilm formation and pyocyanin production of P. aeruginosa PAO1 by 14.42% and 34.35%, respectively. The erasure rate of hordenine to the formed biofilm is 11.05% at 500 μg/ml. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that HMMN downregulates not only siderophore-related genes but also QS-related genes, as well as hordenine. These results suggest that a siderophore inhibitor could be used as a QS inhibitor to occlude the QS system and reduce virulence.
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Advanced Healthcare Materials
·
2022
·Vol. 11
·期 13
·2200299
·
IF 11.092
Jingjing Hu,Sijia Chen,Yongxin Yang,Lin Li,Xuejing Cheng,Yiyun Cheng,Quan Huang
Biofilm is the main culprit of refractory infections and seriously threaten to the human health. Here, a smart hydrogel consisted of norspermidine, aminoglycosides, and oxidized polysaccharide is prepared via the formation of acid-labile imine linkage to treat Pseudomonas aeruginosa biofilm infections in several animal models. The increased acidity caused by bacterial infection triggers the release of norspermidine and aminoglycosides covalently bound with the polymer scaffold. The released norspermidine inhibits biofilm formation and virulence production by regulating the quorum sensing of P. aeruginosa , while the aminoglycoside antibiotics effectively kill the released bacteria. The gel thoroughly inhibits biofilm formation on various medical devices and decreases bacteria pathogenicity. It efficiently inhibits implantation-associated biofilm infections and chronic wound infections, and shows great promise to prevent and treat biofilm-induced refractory infection in clinics.
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