CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Yibo Zhang,Peng Hu,Yaseen Muhammad,Ying Tang,Shan Shao,Zhu Gao,Jiaxing Wang,Ruimeng Wang,Yang Hu,Lihan Kuang,Zhongxing Zhao,Zhenxia Zhao
Hollow nano-sphere NH 2 -MIL88(Fe) (HS NMIL88(Fe)) with interfacial defects was fabricated to load laccase as a host. Through metal multi-coordination, dopamine engineered large amount of defective pores with large size on NH 2 -MIL88(Fe) interface. This defect-interfacial structure provided a high-density host for laccases, which efficiently protected enzyme from structure deformation and then inhibited inactivation. Besides, the unique hollow structure will help to fasten reactant diffusivity, and thus improved the catalytic reaction process. Experimental results showed that the maximum immobilized capacity and maximum activity recovery for laccase reached up to 213.2 mg/g and 75.8% beyond many state-of-the-art enzyme hosts, respectively. Meanwhile, the immobilized laccase exhibited better pH, thermal and storage stabilities on HS NMIL88(Fe) than free and loaded on NMIL88(Fe) ones. Thermal deactivation kinetics analysis showed that HS NMIL88(Fe)-Lac had much higher apparent activation energies of (105.2 kJ/mol) than that of free laccase (89.2 kJ/mol). Catalyzing decolorization performance of dye Remazol Brilliant Blue R (RBBR) was finally carried out and exhibited the decolorization rate of 1.8 times and 2.6 times faster than solid NMIL88(Fe)-Lac and free laccase, respectively. Moreover, the interaction mechanism of laccase for RBBR was investigated and revealed by molecular docking.
研究领域:
生物化学
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环境科学
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材料科学
✓ 已验证
DOI
高影响因子
WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 236
·期 10
·1-14
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IF 3
Liu Jingliang,An Fengxia,Zheng Di,Li Chao,Sun Jingya
In this study, Mn-Ni-layered double hydroxides (Mn-Ni-LDHs) were synthesized and utilized to activate peroxymonosulfate (PMS) for degrading reactive brilliant blue KN-R (KN-R) in aqueous solutions. The characterization results demonstrate that the catalysts synthesized are layered structures, and the surface area is 169.3 m 2 /g. The Mn-Ni-LDHs exhibited significant efficiency effective for the degradation of KN-R via activating PMS, achieving 96.5% degradation of KN-R within 90 min under optimal conditions (0.20 g/L catalyst, 10 mM PMS, pH=10.0 and 25°C). The reaction followed pseudo-first-order kinetics with a rate constant of 0.0351 min -1 , and the apparent activation energy was calculated to be 11.94 kJ/mol. Electron paramagnetic resonance (EPR) and free radical scavenging experiments identified ·O 2 − and 1 O 2 as the primary reactive species involved in the reaction for KN-R degradation. The proposed catalytic mechanism suggests that redox cycling between Mn 4+ /Mn 3+ and Ni 3+ /Ni 2+ within Mn-Ni-LDHs plays a critical role in enhancing PMS activation, thereby promoting the continuous generation of active oxygen species.
研究领域:
环境科学
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化学工程
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材料科学
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水处理技术
✓ 已验证
产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Peipei Zhou,Ying Kuang,Lihua Zuo,Shuhong Liang,Zhuolun Li,Lei Chen,Jingxue Li,Jun Zhang,Chenhan He,Xiaobao Wang,Yaojuan Chu,Jianying Pei,Zhi Sun,Shuzhang Du
Background Danshen Ligustrazine Injection (DSLI), composed of the extract of Salvia miltiorrhiza Bunge and Conioselinum anthriscoides 'Chuanxiong', is commonly used in the clinical treatment of ischemic stroke, which is the most common type of cerebrovascular disease, affected by multiple factors. The brain-gut axis is a new type of influencing factor for ischemic stroke, however, there is a lack of systematic research on the relationship between microbiota and host metabolic phenotype. This study aimed to investigate the host metabolic phenotype-microbiome interface in ischemic stroke and the immunomodulatory effects of DSLI. Methods In the study, liquid chromatography-mass spectrometry was used to primarily analyze the quality markers (Q-markers) and the polyphenols of DSLI. Based on 16S rRNA gene sequencing and metabolomics, the variations of microbial composition in different groups were characterized. In addition, a network diagram was constructed to reveal the interrelationship between DSLI-regulated gut microbiota changes and DSLI-regulated brain metabolites in MCAO/R rats. The expression of the aryl hydrocarbon receptor (AhR) in the colon and brain by immunofluorescence and suppressors of cytokine signaling 2 (SOCS2) by western blot were further detected. Results Diminished metabolic diversity and alterations in the tryptophan metabolism pathway were a feature of IS in the study. The results revealed that Clostri_UCG-014 was positively correlated with indoleacrylic acid, indole-3-methylacetate, and Ruminococcaceae was strongly related with L-tryptophan, 3-methylindole, and indole. The results of immunofluorescence showed that the expression of AhR in the colon and brain tissues of rats in the model group was greatly reduced compared with that in the sham-operated group, and was heavily activated with the treatment of DSLI. The levels of aryl hydrocarbon nuclear receptor ARNT2 and SOCS2 were down-regulated in the brains of Model rats, whereas increased by DSLI, further confirming that the brain-gut microbiota-Trp metabolism exists in the pathological changes of stroke and the intervention mechanism of DSLI. Conclusions The present study findings suggest Clostri_UCG-014, Ruminococcaceae, and Firmicute_RF39 play an important role in improving the regulation of immune function in stroke through tryptophan metabolites by acting on the AhR-SOCS2 axis.
研究领域:
药理学
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神经科学
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微生物学
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代谢组学
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免疫学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Composites and Hybrid Materials
·
2022
·Vol. 5
·期 3
·2138-2153
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IF 11.806
Ding Zhaodong,Tian Zhongjian,Ji Xingxiang,Dai Hongqi,Si Chuanling
Inspired by the hydrophobic waxy layer structure on the back of the desert beetle, a super-hydrophobic material was prepared via the sol–gel silanisation of porous cellulose membrane (CM) using triethoxysilanes with different carbon chain lengths (R-siloxane) and (CH 3 CH 2 O) 4 Ti as a catalyst in 3 min. The R-siloxane CM was both super-hydrophobic and oleophilic (water contact angle (CA) > 150°, dodecane CA ≈ 0°) and resisted liquids with surface tensions as low as 15 mN·m −1 , correlated with the length of the R-siloxane and roughness of the CM. Instructed by the water collection behavior of the desert beetle, a pristine filter CM was added on the R-siloxane filter CM to accelerate separation of Span 80–stabilised water-in-oil emulsions by generating a surface energy gradient (SEG). The composite membrane with SEG separated the emulsions with higher surfactant concentrations and provided a higher efficiency up to 99.98% compared with single-layer R-siloxane membrane. This bio-inspired catalytic one-step approach for preparing R-siloxane cellulose composite membranes can potentially replace petroleum-based products for oil purification. Graphical Abstract Bio-inspired preparation of R-siloxane composite cellulose membrane with surface energy gradient for highly efficient water-in-oil emulsion separation.
研究领域:
材料科学
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化学工程
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环境科学
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仿生学
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Xiaolin Pei,Jiapao Wang,Haoteng Zheng,Qinjie Xiao,Anming Wang,Weike Su
The catalytically-active inclusion bodies (CatIBs) represent a promising strategy for immobilizing enzyme without additional carriers and chemicals, which has aroused great attention in academic and industrial communities. In this work, we discovered two natural parallel right-handed coiled-coil tetramer peptides from PDB database by a structural mining strategy. The two self-assembling peptides, NSPdoT from rotavirus and HVdoT from human Vasodilator-stimulated phosphoprotein, efficiently induced the CatIBs formation of a ( R )-Hydroxynitrile lyase from Arabidopsis thaliana ( At HNL) in Escherichia coli cells. This is convenient to simultaneously purify and immobilize the target proteins as biocatalysts. As expected, HVdoT- At HNL and NSPdoT- At HNL possessed drastically increased tolerance toward lower pH values, which will be very critical to synthesize cyanohydrins under acidic condition for suppressing the non-enzymatic side reaction. In addition. At HNL-CatIBs are produced at high yield in host cells as bioactive microparticles, which exhibited high thermal and pH stabilities. Therefore, the CatIBs method represent a promising application for the immobilization of enzymes in the biocatalysis field.
研究领域:
生物催化
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酶工程
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蛋白质科学
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
Antioxidants
·
2022
·Vol. 11
·期 8
·1530
·
IF 7.675
Qingxiu Liu,Wenxiang Li,Shimeng Huang,Lihong Zhao,Jianyun Zhang,Cheng Ji,Qiugang Ma
The development of single enantiomers with high efficiency and low toxic activity has become a hot spot for the development and application of drugs and active additives. The aim of the present study was to investigate the effectiveness of the application of α-lipoic acid with a different optical rotation to alleviate the inflammation response and oxidative stress induced by oxidized fish oil in laying hens. Sixty-four 124-week-old Peking Red laying hens were randomly allocated to four groups with eight replicates of two birds each. The normal group was fed basal diets supplemented with 1% fresh fish oil (FO), and the oxidative stress model group was constructed with diets supplemented with 1% oxidized fish oil (OFO). The two treatment groups were the S-form of the α-lipoic acid model with 1% oxidized fish oil (OFO + S-LA) and the R-form of the α-lipoic acid model with 1% oxidized fish oil (OFO + R-LA) added at 100 mg/kg, respectively. Herein, these results were evaluated by the breeding performance, immunoglobulin, immune response, estrogen secretion, antioxidant factors of the serum and oviduct, and pathological observation of the uterus part of the oviduct. From the results, diets supplemented with oxidized fish oil can be relatively successful in constructing a model of inflammation and oxidative stress. The OFO group significantly increased the levels of the serum inflammatory factor (TNF-α, IL-1β, IL-6, and IFN-γ) and the oxidative factor MDA and decreased the activity of the antioxidant enzyme (T-AOC, T-SOD, GSH-Px, GSH, and CAT) in the oviduct. The addition of both S-LA and R-LA significantly reduced the levels of serum inflammatory factors (TNF-α, IL-1β, IL-6, and IFN-γ), increased the activity of antioxidant indexes (T-AOC, T-SOD, GSH-Px, GSH, and CAT), and decreased the MDA contents in the serum and oviduct. Meanwhile, the supplementation of S-LA and R-LA also mitigated the negative effects of the OFO on the immunoglobulins (IgA and IgM) and serum hormone levels (P and E2). In addition, it was worth noting that the R-LA was significantly more effective than the S-LA in some inflammatory (IL-1β) and antioxidant indices (T-SOD, GSH, and CAT). Above all, both S-LA and R-LA can alleviate the inflammation and oxidative damage caused by oxidative stress in aged laying hens, and R-LA is more effective than S-LA. Thus, these findings will provide basic data for the potential development of α-lipoic acid as a chiral dietary additive for laying hens.Keywords:S-form of α-lipoic acid;R-form of α-lipoic acid;oxidized fish oil;antioxidant;laying hen
研究领域:
动物科学
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营养学
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免疫学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Chao-ping He,Wen-juan Quan,Ya-ling Zeng,Hong-yan Zhou,Pei-dong You,Zhen-xian Li,Ya-mei Li,Li-mei Lin,Bin Liu,Duan-fang Liao,Qin-hui Tuo
Reducing lipid uptake of macrophages and stimulating cholesterol efflux are two necessary steps for atherosclerotic plaque regression. In this study, a compound of curcumin nicotinate (CN) was synthesized from nicotinic acid which can raise raising high-density lipoprotein (HDL) and curcumin which can lower lipid. However, the shortcomings of CN, such as poor water solubility and low bioavailability, limit its clinical application. In this article, a CN loaded biomimetic nanosystem was constructed by using Prussian blue nanoparticles (PB NPs) to improve its solubility, thereby changing its administration route. In addition, hyaluronic acid (HA) modification on the biomimetic PB NPs was adopted to prolong circulation time and improve the accumulation of drugs in the plaque region. Mechanism studies have shown that the constructed nanosystem could exert anti-AS effects through the pathway of Proprotein Convertase Subtilisin/Kexin Type 9(PCSK9) /Low-density lipoprotein receptor (LDL-R), ATP Binding Cassette Transporter A1(ABCA1) /Caveolin-1 /Liver X Receptor (LXR). These findings indicated that the designed nano-platform is expected to be used for prevention and targeted therapy of atherosclerosis.
研究领域:
纳米技术
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药学
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心血管疾病
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
·
2019
·
IF 3.197
Jianzhong Zhu,Bin Zhang,Bingxu Wang,Chao Li,Xiong Fu,Qiang Huang
Inhibitory effects (IEs) of flavonoids from Rosa roxburghii and R. sterilis fruits against α-glucosidase were investigated. Crude extract of R. roxburghii fruits ( Rr CE) and R. sterilis fruits ( Rs CE) were extracted with ethanol. The n-butanol extraction of aqueous Rr CE dispersion ( Rr CE - B) showed the greatest inhibition of α-glucosidase. The most abundant three components in Rr CE - B were kaempferol hexose, rutin, and catechin (Cat). The predominant active compounds were characterized as flavonoids by UV–vis spectra. Combination analysis suggested that Rr CE was a favorable choice to combine with acarbose (Aca). After simulated gastric digestion, IEs of flavonoids decreased greatly, especially when sodium nitrite was supplemented in the simulated gastric fluid (SGF). The UV–vis spectra indicated that flavonoids were basically stable in the SGF without sodium nitrite, but were oxidized by the HNO 2 , a natural component in the gastric fluid. The results of this study will help food scientists design special foods for diabetic patients.
研究领域:
药理学
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食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 25
·11380-11389
·
IF 3.591
Rui Yang,Yifei Liu,Zhen Dong,Haiyan Li,Zhiwen Ye
As an environmentally friendly energetic group, the azide group can not only control the melting point but also increase the energy of the compound. Therefore, the design and synthesis of energetic compounds with azido groups have long been a focus of research in the field of energetic materials. 3-R-4-(5-methyleneazide-1,2,4-oxadiazol-3-yl)furazan energetic materials were synthesized and fully characterized. Using computational chemistry methods, the interactions of some compounds were studied, and the physical and chemical properties of all of compounds were examined. The results show that 3-amino-4-(5-methyleneazide-1,2,4-oxadiazol-3-yl)furazan (2) has a melting point similar to that of TNT and can be a potential carrier for molten-cast explosives. Hydroxylamine salt (4) has an excellent detonation velocity and detonation pressure of 9090 m s−1 and 35.76 GPa, respectively, and its energy is significantly higher than that of RDX, enabling replacement of RDX as a high-energy explosive. Although the detonation performance of compound 4 is slightly lower than that of HMX, its sensitivity is better than that of HMX, which shows that compound 4 still has a high application value. Non-covalent interactions (NCIs) and Hirshfeld surface models indicate that hydrogen bonding and π–π interactions are the key factors that reduce the sensitivity of compounds. Therefore, our results for 3-R-4-(5-methyleneazide-1,2,4-oxadiazol-3-yl)furazan energetic materials suggest that it may be competitive for application in both molten-cast explosives and high-energy explosives.
研究领域:
化学
·
材料科学
·
爆炸物研究
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2022
·Vol. 70
·期 2
·697-706
·
IF 2.724
Shijin Wu,Bijiao Wang,Hongde Yan
Asymmetric reduction of ( R )-carvone and ketoisophorone by an engineered ene-reductase from Galdieria sulphuraria (GsOYE) combined with glucose dehydrogenase for NADPH regeneration were studied. A semi-rational protein engineering was used to enhance the activity and selectivity of GsOYE. Upon the sequence alignment and molecular docking results, two amino acid residues at positions 66 and 270 were selected as saturation mutation sites. Finally, a single substitution variant of GsOYE-N270A with complete conversion (100%) and diastereoselectivity (de p >99%) for reduction of ( R )-carvone and a double substitution variant GsOYE-Y66P/N270H with improved stereoselectivity for reduction of ketoisophorone were obtained.
研究领域:
生物化学
·
蛋白质工程
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催化
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELL RESEARCH
·
2022
·Vol. 32
·期 7
·638-658
·
IF 46.297
Wang Xiaomin,Chen Ziqi,Xu Jun,Tang Shuai,An Nan,Jiang Lei,Zhang Yixiang,Zhang Shaoying,Zhang Qingli,Shen Yanyan,Chen Shijie,Lan Xiaojing,Wang Ting,Zhai Linhui,Cao Siyuwei,Guo Siqi,Liu Yingluo,Bi Aiwei,Chen Yuehong,Gai Xiameng,Duan Yichen,Zheng Ying,Fu Yixian,Li Yize,Yuan Liang,Tong Linjiang,Mo Kun,Wang Mingcheng,Lin Shu-Hai,Tan Minjia,Luo Cheng,Chen Yi,Liu Jia,Zhang Qiansen,Li Leping,Huang Min
Mutant isocitrate dehydrogenase 1 (mIDH1) drives tumorigenesis via producing oncometabolite R-2-hydroxyglutarate (R-2-HG) across various tumor types. However, mIDH1 inhibitors appear only effective in hematological tumors. The therapeutic benefit in solid tumors remains elusive, likely due to the complex tumor microenvironment. In this study, we discover that R-2-HG produced by IDH1-mutant tumor cells is preferentially imported into vascular endothelial cells and remodels mitochondrial respiration to promote tumor angiogenesis, conferring a therapeutic vulnerability in IDH1-mutant solid tumors. Mechanistically, SLC1A1, a Na + -dependent glutamate transporter that is preferentially expressed in endothelial cells, facilitates the influx of R-2-HG from the tumor microenvironment into the endothelial cells as well as the intracellular trafficking of R-2-HG from cytoplasm to mitochondria. R-2-HG hijacks SLC1A1 to promote mitochondrial Na + /Ca 2+ exchange, which activates the mitochondrial respiratory chain and fuels vascular endothelial cell migration in tumor angiogenesis. SLC1A1 deficiency in mice abolishes mIDH1-promoted tumor angiogenesis as well as the therapeutic benefit of mIDH1 inhibitor in solid tumors. Moreover, we report that HH2301, a newly discovered mIDH1 inhibitor, shows promising efficacy in treating IDH1-mutant cholangiocarcinoma in preclinical models. Together, we identify a new role of SLC1A1 as a gatekeeper of R-2-HG-mediated crosstalk between IDH1-mutant tumor cells and vascular endothelial cells, and demonstrate the therapeutic potential of mIDH1 inhibitors in treating IDH1-mutant solid tumors via disrupting R-2-HG-promoted tumor angiogenesis.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 13
·7062-7074
·
IF 6.317
Fengwei Ma,Qingfang Deng,Huayong Lou,Jinyu Li,Su Xu,Wenneng Wu,Qihua Wen,Lei Tang,Xin Wang,Weidong Pan
Self-heal (Prunella vulgaris L.) is a perennial edible plant that is widely distributed across the world and is traditionally consumed as a food additive in soft drink beverages. In this study, to explore the functional components of P. vulgaris, an investigation of its ethanol extracts has been conducted by our group. As a result, twelve (1–12) vulgarisin-type diterpenoids with a special 5/6/4/5-fused ring skeleton, including six new ones (1–6), were obtained. Their structures including the absolute configuration were elucidated based on comprehensive spectroscopic evidence, ECD calculations, as well as single-crystal X-ray diffraction analyses. All the isolates were tested for neuroprotective effects against ischemia/reperfusion (I/R) on primary neuron cells through the oxygen and glucose deprivation and reperfusion (OGD/R) induced injury model. The results showed that all twelve vulgarisin-type diterpenoids possess promising neuroprotective activity at a concentration of 10 μM. Among them, compound 3 can significantly suppress cell apoptosis by regulating Bax/Bcl-2 protein expression and inhibiting cleaved caspase-3 and caspase-9 expression with a western blotting assay. Further research revealed that compound 3 could improve mitochondrial function by inhibiting mitochondrial cytochrome c release, reducing ROS levels, and maintaining the membrane potential. This work firstly reports vulgarisin-type diterpenoids possessing neuroprotective activity. These findings also suggest that daily consumption of P. vulgaris might prevent cerebral disorders via a mitochondria-related pathway.
研究领域:
药理学
·
神经科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）